Correlation Studies in Segregating Population of Brinjal  (Solanum melongena L.) Developed Through Bi-Parental Mating Using Bacterial Wilt Resistant Varieties

Bacterial wilt caused by Ralstonia solanacearum is one of the major diseases in Solanum species including cultivated Eggplant (Solanum melongena L.). Bacterial wilt (BW) disease management in eggplant is difficult due to high survival rate of pathogen in soil and chemical application is not eco-friendly. The best way to avoid bacterial wilt in eggplant is using disease-resistant varieties. However, only a limited number of bacterial wilt resistant varieties are available and, there is a necessity to identify and/or develop new resistant varieties. In the current study, wild Solanum species, and eggplant cultivated varieties were evaluated against Ralstonia solanacearum, and disease incidence was recorded. The cultivated varieties IIHR-108, Pusa Purple Long and Rampur Local were identified as susceptible, whereas, IIHR-7 and CARI-1 were identified as resistant to bacterial wilt. These resistant wild and cultivated varieties can be used as a root-stock in bacterialwilt disease resistant breeding programmes.

Download Full-text

In silico mining of WRKY TFs through Solanum melongena L. and Solanum incanum L. transcriptomes and identification of SiWRKY53 as a source of resistance to bacterial wilt

Plant Gene ◽

10.1016/j.plgene.2021.100278 ◽

2021 ◽

Vol 26 ◽

pp. 100278

Author(s):

Pallavi Mishra ◽

A.N. Tripathi ◽

Sarvesh P. Kashyap ◽

Mohd Aamir ◽

Kavindra N. Tiwari ◽

...

Keyword(s):

Bacterial Wilt ◽

In Silico ◽

Solanum Melongena ◽

Solanum Incanum ◽

In Silico Mining

Download Full-text

RNA-Seq-derived identification of differential transcription in the eggplant ( Solanum melongena ) following inoculation with bacterial wilt

Gene ◽

10.1016/j.gene.2017.11.003 ◽

2018 ◽

Vol 644 ◽

pp. 137-147 ◽

Cited By ~ 1

Author(s):

Na Chen ◽

Bingwei Yu ◽

Riyue Dong ◽

Jianjun Lei ◽

Changming Chen ◽

...

Keyword(s):

Bacterial Wilt ◽

Solanum Melongena ◽

Rna Seq ◽

Differential Transcription

Download Full-text

Selection and Inheritance of Tomato Resistance against Ralstonia solanacearum

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.35464 ◽

2019 ◽

Vol 23 (1) ◽

pp. 61

Author(s):

Isna Maulida ◽

Rudi Hari Murti ◽

Triwidodo Arwiyanto

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Dominant Gene ◽

Tomato Plants ◽

Breeding Lines ◽

Back Cross ◽

Inheritance Patterns ◽

Randomized Design ◽

Resistant Varieties ◽

Resistant Lines

Ralstonia solanacearum is a plant pathogen causes wilting which is a major obstacle in the cultivation of tomato plants. In plant breeding, knowledge of the source of resistance genes and inheritance patterns is important in the development of bacterial wilt resistant varieties. This study aimed to obtain bacterial wilt resistant lines and to find out the inheritance pattern of tomato resistance to bacterial wilt. Selection of resistant plant involved the selected breeding lines from irradiation and crossing collections of the Genetic Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Introduced lines of H-7996 and F1 Permata and Timoti were used as a control. H-7996 as resistant parents and GM2 as susceptible parents, and their offspring include F1 GM2 x H-7996, F1 reciprocal, F2, Back Cross 1 (F1 x GM2), and Back Cross 2 (F1 x H-7996) used in testing inheritance patterns. Inoculation was carried out 1 week after planting by pouring 100 ml of water suspension of R. solanacarum (108 cfu/ml) on the roots. Completely Randomized Design (CRD) was used in this experiment. The scoring observation was carried out every week for one month. This study showed that Permata as a control was the most resistant, while Timoti and H-7996 were medium resistant. The CLN, G6, G8, and G7 lines were susceptible medium, yet only G8 and G7 with the smallest percentage of disease intensity and not significantly different than Timoti. The resistance gene to bacterial wilt on H-7996 was controlled by genes in the cell nucleus with additive-dominant gene action. Resistance to bacteria has a moderate level of heritability.

Download Full-text

Burkholderia solanacearum. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056401220 ◽

1994 ◽

Author(s):

G. S. Saddler

Keyword(s):

Geographical Distribution ◽

Bacterial Wilt ◽

Vascular System ◽

Solanum Melongena ◽

Zingiber Officinale ◽

Modes Of Transmission ◽

Planting Material ◽

Complete Listing ◽

Latently Infected ◽

Plant Families

Abstract A description is provided for Burkholderia solanacearum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: The host range is one of the widest of all the phytopathogenic bacteria. The most susceptible plant family, in terms of numbers of species affected is the Solanaceae; over fifty other plant families contain susceptible species. The most economically significant hosts are listed here. For a more complete listing see Kelman (1953), Bradbury (1986) and Hayward & Hartman (1994). Arachis hypogaea, Capsicum spp., Gossypium hirsutum, Ipomoea batatus, Lycopersicon esculentum, Manihot esculenta, Musa spp., Nicotiana spp., Solanum melongena, Solanum tuberosum & Zingiber officinale. DISEASE: Bacterial wilt. Infection is systemic, producing a wilt of parts or the whole plant. Vascular system may become discoloured, bacterial ooze can be produced and plants may be stunted and chlorotic. GEOGRAPHICAL DISTRIBUTION: Widespread in tropical, subtropical and warm temperate regions of the world (see data sheets on quarantine pests). TRANSMISSION: A variety of modes of transmission exist which are host dependant. Infected planting material and true seed is responsible for the spread of bacterial wilt of banana, ginger, groundnut, potato and tomato, whilst transport of latently infected in seedlings (stawberry) and the actions of insect (Moko disease of banana) and weather (tobacco) have all been implicated. For a review see Kelman et al. (1994).

Download Full-text

Screening Cultivated Eggplant and Wild Relatives for Resistance to Bacterial Wilt (Ralstonia solanacearum)

Agriculture ◽

10.3390/agriculture9070157 ◽

2019 ◽

Vol 9 (7) ◽

pp. 157 ◽

Cited By ~ 3

Author(s):

Namisy ◽

Chen ◽

Prohens ◽

Metwally ◽

Elmahrouk ◽

...

Keyword(s):

High Temperatures ◽

Resistance Genes ◽

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Solanum Melongena ◽

Wild Relatives ◽

Sources Of Resistance ◽

Race 1 ◽

Moderately Resistant ◽

Phylotype I

Bacterial wilt, caused by Ralstonia solanacearum, is highly diverse and the identification of new sources of resistance for the incorporation of multiple and complementary resistance genes in the same cultivar is the best strategy for durable and stable resistance. The objective of this study was to screen seven accessions of cultivated eggplant (Solanum melongena L.) and 40 accessions from 12 wild relatives for resistance to two virulent R. solanacearum strains (Pss97 and Pss2016; phylotype I, race 1, biovar 3). The resistant or moderately resistant accessions were further evaluated with Pss97 in a second trial under high temperatures (and also with Pss2016 for S. anguivi accession VI050346). The resistant control EG203 was resistant to Pss97, but only moderately resistant to Pss2016. One accession of S. sisymbriifolium (SIS1) and two accessions of S. torvum (TOR2 and TOR3) were resistant or moderately resistant to Pss97 in both trials. Solanum anguivi VI050346, S. incanum accession MM577, and S. sisymbriifolium (SIS1 and SIS2) were resistant to Pss2016 in the first trial. However, S. anguivi VI050346 was susceptible in the second trial. These results are important for breeding resistant rootstocks and cultivars that can be used to manage this endemic disease.

Download Full-text

Biovar Differentiation and Variation in Virulence of Ralstonia solanacearum Isolates Infecting Solanaceous Vegetables

Journal of Nepal Agricultural Research Council ◽

10.3126/jnarc.v2i0.16117 ◽

2016 ◽

Vol 2 ◽

pp. 22-26 ◽

Cited By ~ 1

Author(s):

Ram Devi Timila ◽

Shrinkhala Manandhar

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Negative Impact ◽

Virulent Strain ◽

Solanum Melongena ◽

Susceptible Variety ◽

Resistant Variety ◽

Slight Variation ◽

Solanaceae Family ◽

Moderately Resistant

Bacterial wilt caused by Ralstonia solanacearum E.F. Smith is one of the destructive diseases of solanaceous vegetables specially tomato (Lycopersicon esculentum L.) and eggplant (Solanum melongena L.). Experiments were conducted to determine biovar types existing among the strains or isolates of Nepal and variation in virulence in some vegetables belonging to solanaceae family. A total of 39 isolates infecting tomato, eggplant, chilli and potato collected from different parts of Nepal were analyzed for biovar types on the basis of 3 disaccharides and 3 hexose alcohols oxidation test. Experiments were conducted to determine variation in virulence or aggressiveness of some of the isolates under screen house conditions using three host differentials such as Pusa Ruby (susceptible), Bishesh (moderately resistant) and Srijana (resistant) tomato cultivars. Of the 39 isolates, 23 were biovar III, three biovar II, three biovar IV, and one was biovar I. Nine isolates could not be differentiated into any of the five biovars. For breeding and epidemiological purposes it is very important to analyze the variability of aggressiveness. A total of 5 isolates collected from different places were included in the test. Isolates from Bhaktapur was found the most virulent causing wilt in the variety Bishesh (moderately resistant). Other isolates had the negative impact with zero wilt on the differentials used. Isolates from Jungekhola of Dhading district did not induce wilt even on susceptible variety (Pusa Ruby), but exhibited only senescence reaction. The result indicated that there is some slight variation among the isolates tested. Some effective management tactics might be needed in those locations where highly aggressive or virulent strain of bacterial wilt is prevalent, because resistant variety may not be stable in such locations.

Download Full-text