scholarly journals Isolation and Identification of Phenol Degrading Halophilic Bacteria from Lonar Lake

2018 ◽  
Vol 7 (1) ◽  
pp. 2501-2508
Author(s):  
B.A. Mehere ◽  
Priyanka A. Gupta ◽  
Manashree D. Dongre
Keyword(s):  
Halophilic Bacteria ◽  
Isolation And Identification ◽  
Lonar Lake

scholarly journals Isolation and identification of halophilic bacteria producing halotolerant protease

2017 ◽  
Vol 1 (1) ◽  
pp. 7-9 ◽  
Author(s):  
Nadiah Syuhada Abd Samad ◽  
Azura Amid ◽  
Dzun Noraini Jimat ◽  
Nurul Aqilah Ab. Shukor
Keyword(s):  
Halophilic Bacteria ◽  
Isolation And Identification

scholarly journals Isolation and identification of halophilic and halotolerant bacteria from the sediments of the Qeshm Island mangrove forest

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Pegah Javid ◽  
Hassan Zadabbas Shahabadi ◽  
Homeyra Amirkhani ◽  
Narges Amrollahi ◽  
Mohammad Sharif Ranjbar
Keyword(s):  
Halophilic Bacteria ◽  
Mangrove Forests ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Qeshm Island ◽  
Gram Positive ◽  
Halotolerant Bacteria ◽  
Gram Positive Bacteria ◽  
Phylogenetic Taxonomy

Due to specific environmental and ecological conditions, mangrove forests are known as marine transitional zones between sea and land, and, as such, they host organisms with high ecological plasticity. The mangrove forests of Qeshm Island (Iran) are relatively pristine habitats and represent an ideal target for investigating patterns of either aquatic or benthic biodiversity. To provide insights on microbial diversity in this area, nineteen halophilic and halotolerant bacteria were isolated from the sediments in 2017 during low tide. The extracted bacterial strains were studied morphologically by streaking, initial observation of colonies and bacterial staining, and characterized using a battery of biochemical tests including KOH, MR, VP, urease, TSI, S/I/M, Mac, LIA, ODC, ADH, oxidase, catalase, and tryptophan deaminase. The optimum growth of halophilic bacteria was observed in salt concentrations from 5 to 20% NaCl, whereas the extreme halophilic Gram-positive strain grew in salt concentration of up to 30% NaCl. Molecular analyses were also carried out on four halophilic strains and one extreme halophilic gram-positive bacteria. Phylogenetic taxonomy analysis, after 16S rDNA gene Sanger sequencing, revealed that the halophilic bacteria were closely related to the strain types of the genus Bacillus including Bacillus licheniformis, Bacillus velezensis, Bacillus Paralicheniformis and Bacillus sp. with 99% bootstrap value. The extreme halophilic strain was associated to strains of Planococcus plakortidis with 100% bootstrap value.

scholarly journals Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran

2019 ◽  
Vol 25 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Babak Elyasifar ◽  
Sevda Jafari ◽  
Somayeh Hallaj-Nezhadi ◽  
Florence Chapeland-leclerc ◽  
Gwenaël Ruprich-Robert ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Halophilic Bacteria ◽  
Pathogenic Fungi ◽  
Antibacterial Activities ◽  
Biochemical Properties ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Isolation And Identification ◽  
Phylogenic Tree

Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered in drug innovation and control of plant pathogens. Salt deserts in Semnan province are of the most permanent hypersaline areas in the North of Iran. Despite the importance of these areas, there is no scientific report regarding the biodiversity and potency of their halophilic bacteria. Thus, aforementioned areas were selected to detect the halophilic bacteria. Methods: Here, seven strains were isolated and cultured on their molecular and biochemical properties were characterized. To determine the antibiotic potency of the isolates, agar well diffusion method was conducted. Phylogenetic analysis was done to reveal the isolates relationship with previously known strains. Results: As a result, growth of the strains in the medium containing 5 to 20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. Catalase activity of all strains was positive. The results represented that D6A, Dar and D8B have antimicrobial effects against different plant and human pathogens. Phylogenic tree analysis also showed that two strains of D6A and Dar are belonged to Bacillus subtilis and D8B is belonged to Virgibacillus olivae. The bacteria extracts were evaluated for their antifungal and antibacterial activities on human and Plant pathogenic strains. The MIC of the extract B. subtilis against was found active against human pathogenic fungi and Plant pathogenic bacteria and fungi, ranging from 12.5 to 25 µg/mL. Conclusion: This study highlights the therapeutic and prophylactic potential of B. subtilis extracts as antibacterial and antifungal agents.

Isolation and Identification of Halomonas sp. ZSCW-10: A Moderately Halophilic Bacteria Strain with Cellulase Activity

2013 ◽  
Vol 749 ◽  
pp. 236-241 ◽  
Author(s):  
Wei Qing Chen ◽  
Yan Yan Liu
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rdna ◽  
Halophilic Bacteria ◽  
Cellulase Activity ◽  
Evolutionary Genetics ◽  
Biochemical Properties ◽  
Isolation And Identification ◽  
Moderately Halophilic ◽  
Wide Range ◽  
Moderately Halophilic Bacteria

A moderately halophilic bacteria strain with cellulase (CMCase) activity, ZSCW-10, which can survive under wide range of NaCl concentration (1 %~15 %, w/v) and pH (4.5~9.0), was isolated from the sediment of intertidal zone located in the Xiangshan Island, Zhejiang Province. The strain was identified based on 16S rDNA sequence determination and phylogenetic analysis together with its morphological and physiological characterization. The results indicated that the 16S rDNA homologies were 97% between strain ZSCW-10 and some strains of Halomonas sp.. The phylogenetic tree was constructed with Molecular Evolutionary Genetics Analysis version 5 (MEGA5), showed the closest relationship between strain ZSCW-10 and Halomonas venusta. Based on its physiological and biochemical properties, homology and phylogenetic analysis, strain ZSCW-10 was identified as a subspecies of Halomonas sp.. The optimum culture conditions were: NaCl 6.0%, 32°C, pH 7.5.

scholarly journals F1FoATP synthase a-subunit of Stenotrophomonas sp. DL18 from Indian Soda Lake, Lonar: a brief report

F1000Research ◽  
2013 ◽  
Vol 1 ◽  
pp. 62
Author(s):  
Devendra Lingojwar ◽  
Ravikant Jadhav ◽  
Kachru Gawai
Keyword(s):  
Ribosomal Rna ◽  
Present Report ◽  
Soda Lake ◽  
Amino Acid Residues ◽  
Isolation And Identification ◽  
Specific Amino Acid ◽  
Molecular Studies ◽  
Alkaline Conditions ◽  
Lonar Lake ◽  
A Subunit

Lonar Lake, an Indian soda lake with high alkaline conditions of pH 10.5, is well known for its biodiversity of extremophiles including alkaliphiles. Most of the molecular studies on Lonar Lake alkaliphiles are based on identification by 16S ribosomal RNA (16S rRNA). Various studies have reported alkaliphiles from different alkaline habitats other than Lonar Lake with alkaliphile specific amino acid residues in the F1FoATP synthase a-subunit. As the data on the alkaliphilic nature of bacteria from Lonar Lake is incompletely understood, the present report comprised of isolation and identification of alkaliphiles from Lonar Lake. Further, we studied the F1FoATP synthase a- subunit, with reference to alkaliphile specific domains, of one of the facultative alkaliphiles, Stenotrophomonas sp. DL18.

Isolation and identification of moderately halophilic bacteria producing hydrolytic enzymes from the largest hypersaline playa in Iran

Microbiology ◽  
2013 ◽  
Vol 82 (4) ◽  
pp. 466-474 ◽  
Author(s):  
Hamid Babavalian ◽  
Mohammad Ali Amoozegar ◽  
Ahmad Ali Pourbabaee ◽  
Mehrdad Moosazadeh Moghaddam ◽  
Fatemeh Shakeri
Keyword(s):  
Halophilic Bacteria ◽  
Hydrolytic Enzymes ◽  
Isolation And Identification ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacteria

Isolation and Identification of Microbes in High-Salinity Environmental Microorganism in Disposal of Salt-Contained Wastewater

2014 ◽  
Vol 608-609 ◽  
pp. 1094-1097
Author(s):  
Chun Yan Zhang ◽  
Hui Liu ◽  
Hai Xing Hu
Keyword(s):  
Natural Environment ◽  
High Salinity ◽  
Salt Lake ◽  
Human Life ◽  
Rapid Development ◽  
Halophilic Bacteria ◽  
Strong Acid ◽  
Social Value ◽  
Toxic Substances ◽  
Isolation And Identification

Along with the rapid development of today's society, all sorts of wastes produced from human life and production are discharged into the natural environment, so that a certain impact has been exerted on air, soil, lakes, and oceans. The wastes discharged into the natural environment not only contain some toxic substances and various compounds difficult to degrade, but also are with the characteristics of strong acid, strong alkali, high temperature, and high salinity, so that the general microorganisms are hard to survive in this environment. However, there are some extreme microorganisms to gradually fit the environment in the process of evolution, and the microorganisms include alkalophilic bacteria, basophilic bacteria, halophilic bacteria, etc. Halophilic bacteria grow mainly in sea and salt lake, and also have unique physiological features, while the application of high-salinity microbes to the disposal of salt-contained wastewater is of a broad prospect and an important social value. In this paper, the isolation and identification of microorganism in a high-salinity environment and their application to the disposal of salt-contained wastewater are mainly analyzed.

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