scholarly journals Antibiotic Susceptibility Pattern and Multiple Antibiotic Resistance Index of Salmonella enterica Isolates from Horses in Bikaner

2021 ◽  
Vol 10 (2) ◽  
pp. 134-140
Author(s):  
T. C. Nayak A. Chahar ◽  
V. Singhal Savita ◽  
R. Yadav T. Maharshi
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Antibiotic Susceptibility ◽  
Resistance Index ◽  
Multiple Antibiotic Resistance ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern

scholarly journals Antibiotic susceptibility pattern of Escherichia coli isolated from food, cooking utensils and palms of food handlers in some restaurants in Zaria, Nigeria

2021 ◽  
Vol 1 (2) ◽  
pp. 28-34
Author(s):  
Victoria Etalong ◽  
Babajide A. Tytler ◽  
Igba Profit ◽  
Adeshina O. Gbonjubola
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Resistance Index ◽  
Major Public Health Problem ◽  
Multiple Antibiotic Resistance ◽  
Food Handlers ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
E Coli

Foodborne disease is a major public health problem causing considerable morbidity and mortality annually. In the present study, the antibiotic susceptibility patterns of Escherichia coli isolated from food, cooking utensils and palms of food handlers in some restaurants in Zaria, Nigeria were evaluated. A total of 250 samples (220 food samples, 7 hand samples of food handlers, 10 plate samples within restaurants and 13 spoon samples) were collected from five locations in Zaria, Nigeria and analysed for microbial contaminations using standard microbiological techniques. The antibiotic susceptibility pattern of the isolates was determined using Kirby-Bauer modified disc agar diffusion technique. Results revealed that out of 158 acclaimed Enterobacteriaceae isolates evaluated, 19 % (30) were confirmed to be E. coli, while 81 % were Klebsiella. spp, Citrobacter fruendii, Enterobacter spp, Shigella spp, Salmonella spp, Serratia spp, and Cronobacter sakazaki. The majority of the isolates were resistant to amoxiclav (26.08%), ampicillin (26.08%), tetracycline (26.08%) and metronidazole (13.04%). A 33.3% of the isolates were multidrug-resistant. The E. coli isolates were mostly multiple antibiotic resistance with 43.3% having multiple antibiotic resistance index (MARI) ≥ 0.2. In conclusion, E. coli evolved resistance to ampicillin, Amoxicillin Clavulanic acid, and Tetracycline and other tested antimicrobial drugs which would make the treatment of Escherichia coli infections difficult

scholarly journals ANTIBIOTIC RESISTANCE IN BACTERIAL PATHOGENS ISOLATED FROM CATTLE DUNG AND ITS CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 66-83
Author(s):  
I. S. Obuekwe ◽  
C. K. Offodile
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Contaminated Soil ◽  
Antibiotic Susceptibility ◽  
Bacterial Pathogens ◽  
Diffusion Method ◽  
Cattle Dung ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Bacillus Spp

  Cattle dung is used as organic fertilizer and alternative source of fuel or biogas but could also be a source of antibiotic resistance genes in the environment. This study isolated, identified and assessed antibiotic susceptibility pattern of bacteria from cattle dung and its contaminated soil. Bacteria isolation and identification were based on standard techniques while hemolytic activity was used to confirm potential pathogenic bacteria. Antibiotic susceptibility pattern of isolated pathogens were assayed by disk diffusion method. Among isolated bacteria, Staphylococcus spp had highest occurrence of 23.8 % while Micrococcus spp was the least at 1.3 %. Hemolytic bacteria isolates were Staphylococcus aureus (16.5 %), Bacillus spp (17.4 %), Nocardia spp (4.6 %), Escherichia coli (29.4 %), Pseudomonas spp (13.8 %), Serratia marcersens (2.8 %) and Salmonella spp (15.6 %). High resistance (100 %) against Ampiclox (30 µg) was observed in all Staphylococcus aureus and Bacillus spp isolates while Pseudomonas aeruginosa isolates showed 100 % resistance to Ofloxacin (30 µg). Most Gram-positive bacterial isolates were majorly resistant to Beta lactams while Gram negative bacteria were resistant to Fluoroquinolones antibiotics. Multiple antibiotics resistant index (MARI) was measured at greater than 0.2, and was observed in 71.5 % of the hemolytic pathogens. Antibiotics resistance in hemolytic bacterial pathogens from this study is indicative of environmental sources of antibiotic resistance and possible adverse effects on human health.

Prevalence and antibiotic susceptibility pattern of Escherichia coli in cattle on Bathan and intensive rearing system

2017 ◽  
Vol 6 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Mukta Das Gupta ◽  
Mazharul Islam ◽  
Arup Sen ◽  
Md Samun Sarker ◽  
Ashutosh Das
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
Antibiotic Susceptibility Pattern ◽  
E Coli ◽  
Intensive Farming

The aim of this longitudinal study was to verify the prevalence and antibiotic susceptibility pattern of Escherichia coli (E. coli) in cattle reared on Bathan and intensive farming system in Bangladesh. Fecal materials originated from recto anal junction (RAJ) of 100 cattle used for primary screening on MacConkey agar. The diversities among the pink color colony producing isolates on MacConkey agar were verified by conventional cultural methods and biochemical tests. Phenotypically positive E. coli isolates were further investigated for the variations in the antimicrobial resistance profiles to 10 selected antibiotics, by the disk-diffusion method. This study revealed that the overall prevalence of E. coli was 70% of in the rectal swab sample of cattle. However, the prevalence of E. coli was found significantly higher (p= 0.002) in cattle under intensive farming (84%) than cattle on Bathan (56%). Antibiotic susceptibility pattern shows that among the tested isolates 83%, 73%, 68% and 64% were sensitive to chloramphenicol, gentamicin, ciprofloxacin and ampicillin, respectively. On the other hand, all the 70 (100%) E. coli isolates were found resistant to tetracycline and sulfamethoxazole. A high antibiotic resistance profile was also found against amoxicillin (90%), ampicillin (87%), nalidixic acid (86%) and erythromycin (83%). In total, 24 (34%) isolates were resistant against ?2 antimicrobials. The result clearly shows that antibiotic resistant E. coli isolates are commonly present in cattle of different management systems (intensive and Bathan). Therefore, careful selection of appropriate antibiotics with optimal doses might be ensured to prevent the emergence of antibiotic resistance bacteria.Microbes and Health, June 2017, 6(1): 1-4

Prevalence and antibiotic susceptibility of bacteria isolated in patients affected with blepharitis in a tertiary eye centre in Spain

2019 ◽  
Vol 30 (5) ◽  
pp. 991-997
Author(s):  
Alessandro de Paula ◽  
Gloria Oliva ◽  
Rafael Ignacio Barraquer ◽  
Maria Fideliz de la Paz
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Blood Agar ◽  
Coagulase Negative Staphylococcus ◽  
Susceptibility Pattern ◽  
Erythromycin Resistance ◽  
Antibiotic Susceptibility Pattern ◽  
Resistance To Penicillin ◽  
And Staphylococcus Aureus

Purpose: To describe which bacteria can be found on lid margins in patients affected with blepharitis, to show their antibiotic susceptibility pattern, and to evaluate the antibiotic resistance trend of coagulase-negative Staphylococcus through time. Materials and methods: Consecutive cases of 198 eyes affected with blepharitis between 2012 and 2018 were reviewed. A sample was collected by rubbing a swab against the base of the eyelashes of both the eyes of all patients. The samples were inoculated in blood agar and chocolate agar. The susceptibility of the identified bacteria to common antibiotics was tested. In addition, the antibiotic susceptibility pattern of coagulase-negative Staphylococcus detected from year 2016 to 2018 was compared with that of 4 years before. Results: The most common isolated bacterium was coagulase-negative Staphylococcus (89%) and Staphylococcus aureus (28%). Coagulase-negative Staphylococcus showed highest susceptibility to vancomycin (100%), neomycin (94%) and chloramphenicol (91%). Coagulase-negative Staphylococcus and Staphylococcus aureus were the most resistant to penicillin and erythromycin (resistance in 92%, 91% for coagulase-negative Staphylococcus, 86% and 43% of eyes for Staphylococcus aureus). Corynebacterium was resistant to oxacillin and erythromycin. Streptococcus viridans showed resistance to gentamycin and tobramycin. Moraxella was susceptible to most antibiotics. Bacillus was resistant to oxacillin. The antibiotic resistance trend of coagulase-negative Staphylococcus showed that the resistance to rifampicin increased through the years 2012–2018. Conclusion: Coagulase-negative Staphylococcus and Staphylococcus aureus were the most isolated bacteria in patients affected by blepharitis in our tertiary eye centre. Both bacteria were resistant to erythromycin. Through the years, it seems that coagulase-negative Staphylococcus gained resistance to penicillin, erythromycin, ciprofloxacin and rifampicin.

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