scholarly journals Polycyclic Aromatic Hydrocarbons (PAH) in Fingerlings of Clarias gariepinus (Burchell, 1822) to Petroleum

2018 ◽  
Author(s):  
Christopher Onyemaechi Ezike ◽  
Felix Okaliwe Echor
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Oil Spills ◽  
Clarias Gariepinus ◽  
Diesel Oil ◽  
Exposed Fish ◽  
Significant Difference ◽  
Polycyclic Aromatic ◽  
Experimental Treatments

One hundred and twenty (120) fingerlings of Clarias gariepinus (mean weight: 0.96 ± 0.1g) were randomly exposed to 4 experimental treatments of petroleum, based on LC50 values (6.4mg/L of crude oil, 8.7mg/L of petrol, 8.0mg/L of kerosene and 7.8mg/L of diesel oil) and replicated thrice, to determine polycyclic aromatic hydrocarbons (PAH) in exposed fish for 96 h. There was no significant difference (P > 0.05) in total (PAHs) between crude oil (97.1 ng/uL) and diesel (97.2 ng/uL) exposed fish and also between petrol (53.2 ng/uL) and kerosene (49.6 ng/uL) exposed fish, but there was a significant difference (P < 0.05) in PAH levels of the crude oil/diesel exposed -groups of fish compared to petrol/kerosene exposed -groups of fish (97.1/97.2 and 53.2/49.6 ng/uL). Naphthalene correlated positively to benzo a anthracene (r=0.672, (P < 0.05), benzo b fluoranthene (r=0.681, P < 0.05) and chrysene (r=0.615, P < 0.05) but did not correlate to fluorene. Benzo a anthracene correlated positively to benzo a pyrene (r=0.578, P < 0.05), phenathrene (r=0.685, P < 0.05) but did not correlate to acenaphthene. Fluorene correlated positively to benzo a pyrene (r=0.695, P < 0.05) but did not correlate to chrysene. Chrysene correlated positively to dibenzo a,h, pyrene (r=0.658, P < 0.05) to phenathrene and benzo b fluoranthene (r=0.659, P< 0.05). Indeno 123 cd- pyrene and fluranthene however did not correlate to other PAHs except naphthanene, acenaphthene and acenaphthylene. The level of PAH in fish may translate to the toxicity effect since crude oil and diesel with lower LC50 (6.4 and 7.8 mg/L)   deposited greater PAH than kerosene and petrol with higher LC50 (8.7 and 8.0 mg/L) in fingerlings of C. gariepinus. High risk to cancer disorders may occur in exposed fish to petroleum with high incidence of fluorene , anthracene, pyrene and benz a anthracene which correlated positively to benzo a pyrene which provide some basis for predicting impact of oil spills on fingerling population.

scholarly journals Assessment of the biodegradation capacity of Azolla on polycyclic aromatic hydrocarbons in crude oil

2018 ◽  
Vol 20 (3) ◽  
pp. 465-470
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Oil Spills ◽  
Dependent Manner ◽  
Measured Intensity ◽  
Azolla Filiculoides ◽  
Concentration Dependent Manner ◽  
Plant Samples ◽  
Polycyclic Aromatic

<p>In this study, the potential use of Azolla filiculoides Lam. for the bioremediative solution to polycyclic aromatic hydrocarbon (PAH) pollution due to crude oil spills in freshwater was investigated. The plants were grown in nitrogen-free Hoagland nutrient solution media containing 0.05%, 0.1%, 0.2%, 0.3%, 0.4% and 0.5% crude oil by volume for 15 days under greenhouse conditions. Relative growth rates of A. filiculoides decreased in the presence of crude oil in a concentration-dependent manner. The probable influence of A. filiculoides on the biodegradation of polycyclic aromatic hydrocarbons was measured by using synchronous UV fluorescence spectroscopy. GC-MS analysis were also carried out to elucidate the behavior of the oil in experimental and control samples. Although 1-2 rings PAHs have not been encountered in control or plant samples, the measured intensity for 3-4 ring PAHs in plant samples was remarkably lower in comparison to the control. Furthermore, these results demonstrated that the predominant efficacy of the A. filiculoides was for 3-4 ring PAHs at the range 0.05 to 0.2% crude oil concentrations. It could be concluded that the bioremediative potential of A. filiculoides for the removal of polycyclic aromatic hydrocarbons strongly depends on the amount of oil in the contaminated water resource. In other words, A. filiculoides could be used more effectively after the removal of excess crude oil in the spilled freshwater areas.</p>

scholarly journals Effective Removal of Alkanes and Polycyclic Aromatic Hydrocarbons by Bacteria from Soil Chronically Exposed to Crude Petroleum Oil

2021 ◽  
Author(s):  
Eman Afkar ◽  
Aly M. Hafez ◽  
Rashid I.H. Ibrahim ◽  
Munirah Aldayel
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Oil Spills ◽  
Practical Significance ◽  
Gas Chromatography Mass Spectrometry ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Polycyclic Aromatic ◽  
Long Chain

Abstract In this study, two bacterial strains isolated from an oil-contaminated soil, designated as AramcoS2 and AramcoS4 were able to degrade crude oil, long-chain n-alkanes of C10 to C20; (n-decane, n-undecane, n-dodecane, n-tridecane, n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane n-nonadecane, and n-eicosane) and polycyclic aromatic hydrocarbons (PAHs) including biphenyl, naphthalene, and anthracene. Gas chromatography-mass spectrometry (GC-MS) technique was conducted to analyze and identify the crude oil residues after biodegradation. AramcoS2 and AramcoS4 were able to reduce the concentration of long-chain n-alkanes of C10-C20 efficiently on average by 77% of the original concentration. Both isolates could also degrade PAHs on average by 67% of the original concentration within 7 and 14 days of incubation at 30ºC, pH=6.8±0.2. The 16S rRNA gene sequences of AramcoS2 and S4 classified these isolates as Actinobacteria; well-known alkanes and PAHs degraders. The nucleotide sequences of AramcoS2 and AramcoS4 were submitted to the GenBank database under the accession numbers MN142506 and MN142551, respectively. Both isolates can be used to restore the environments contaminated with crude oil components. They should be of great practical significance both in bioremediation of soil contaminated with crude oil and bio-treatment of oil spills on surface water.

scholarly journals Characterization of Polycyclic Aromatic Hydrocarbons and Bioaugmentation Potential of Locally Isolated Beneficial Microorganisms Consortium for Treatment of Tar-Balls

2021 ◽  
Vol 19 (3) ◽  
pp. 246-254
Author(s):  
Nur Zaida Zahari ◽  
◽  
Erma Hani Baharudzaman ◽  
Piakong Mohd Tuah ◽  
Fera Nony Cleophas ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Microbial Population ◽  
Oil Spills ◽  
Beneficial Microorganisms ◽  
Tar Balls ◽  
Polycyclic Aromatic ◽  
Biodegradation Study ◽  
Bioreactor System

Oil spills are one of the environmental pollutions that commonly occur along coastal areas. Tar-balls are one of the products that come from the oil spill pollution. In this study, tar-ball pollution was monitored at 10 points along the coastline of Marintaman Beach in Sipitang, Sabah, Malaysia. This research determined the physical characteristics, composition, and concentration of Polycyclic Aromatic Hydrocarbons (PAHs) in tar-balls. The total number of tar-balls collected was 227 (n=227). The tar-balls were observed in various shapes and the sizes were recorded in the range of 0.1 cm to 6.9 cm. The composition and concentration of Polycyclic Aromatic Hydrocarbons (PAHs) in the outer and inner layer of tar-balls were determined. The results showed that the main Polycyclic Aromatic Hydrocarbons (PAHs) compounds were found in inner layers of the tar-balls with benzo (g,h,i) perylene (72.26 mg/kg), flourene (59.87 mg/kg), dibenzo (a,h) anthracene (44.48 mg/kg), indeno (1,2,3-c,d) pyrene (78.18 mg/kg), and benzo (e) fluoranthene (45.70 mg/kg), respectively. Further research was done with the bioaugmentation study of locally isolated beneficial microorganisms (LIBeM) consortium for treatment of tar-balls in an Aerated Static Pile (ASP) bioreactor system. The results showed that, after 84 days of treatment, this consortium, consisting of C. tropicalis-RETL-Cr1, C. violaceum-MAB-Cr1, and P. aeruginosa-BAS-Cr1, was able to degrade total petroleum hydrocarbon (TPH) by 84% as compared to natural attenuation (19%). The microbial population of this consortium during the biodegradation study is also discussed in this paper.

scholarly journals Stenotrophomonas sp. Pemsol isolated from crude oil contaminated soil in Mexico that can degrade polycyclic aromatic hydrocarbons and its whole genome sequence analyzed

2019 ◽  
Author(s):  
Temidayo O Elufisan ◽  
Isabel C Rodríguez-Luna ◽  
Omotayo O Oyedara ◽  
Alejandro Sánchez-Varela ◽  
Armando Hernandez Mendoza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Genome Analysis ◽  
Contaminated Soil ◽  
Antimicrobial Agents ◽  
Whole Genome ◽  
Chromatography Mass Spectrometry ◽  
Polycyclic Aromatic

Background: Stenotrophomonas are ubiquitous gram-negative bacteria which survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation and antimicrobial agents because of their recalcitrant nature to many toxic compounds. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAHs) (anthracene, anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine and xylene) was evaluated on Bush Nell Hass medium containing PAHs as the unique carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). Results. Complete degradation of naphthalene at a concentration of 1 mg/mL was obtained and a newly formed catechol peak obtained from the UPLC-MS and GC-MS confirmed the degradation. The strain Pemsol lacked the ability to produce biosurfactant so that it cannot bio-emulsify PAHs. The whole genome analysis of Stenotrophomonas sp. Pemsol revealed a wealth of genes for hydrocarbon utilization and interaction with the environment and the presence of 147 genes associated with the degradation of PAHs, some of which are strain-specific on the genomic islands. Few genes are associated with bio-emulsification indicated that Pemsol without biosurfactant production has a genetic basis. This is the first report of the complete genome analysis sequence of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that makes it a good bacterium for genetic engineering and will therefore be a good tool for the remediation of crude oil or PAH-contaminated soil.

Potential microbial drivers of biodegradation of polycyclic aromatic hydrocarbons in crude oil sludge using a composting technique

2020 ◽  
Vol 95 (5) ◽  
pp. 1569-1579 ◽  
Author(s):  
Linda Obi ◽  
Harrison Atagana ◽  
Rasheed Adeleke ◽  
Mphekgo Maila ◽  
Emomotimi Bamuza‐Pemu
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Oil Sludge ◽  
Polycyclic Aromatic ◽  
Crude Oil Sludge

scholarly journals Biomarker responses and accumulation of polycyclic aromatic hydrocarbons in Mytilus trossulus and Gammarus oceanicus during exposure to crude oil

2020 ◽  
Vol 27 (13) ◽  
pp. 15498-15514 ◽  
Author(s):  
Raisa Turja ◽  
Steinar Sanni ◽  
Milda Stankevičiūtė ◽  
Laura Butrimavičienė ◽  
Marie-Hélène Devier ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Mytilus Trossulus ◽  
Polycyclic Aromatic
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