scholarly journals Adipophilin and Perilipin 3 Correlate with Total Lipid Content in Human Breast Milk

2018 ◽  
Author(s):  
Tereza Pavlova ◽  
Zdenek Spacil ◽  
Veronika Vidova ◽  
Filip Zlamal ◽  
Eliska Cechova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Breast Milk ◽  
Total Lipid ◽  
Lipid Content ◽  
Gravimetric Method ◽  
Total Lipid Content ◽  
Milk Lipid ◽  
Human Breast Milk ◽  
Human Breast

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods & Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1–3, 12–14, 29–30, 88–90 and 178–180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

scholarly journals Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tereza Pavlova ◽  
Zdenek Spacil ◽  
Veronika Vidova ◽  
Filip Zlamal ◽  
Eliska Cechova ◽  
...  
Keyword(s):  
Breast Milk ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Human Breast Milk ◽  
Human Breast

scholarly journals Analysis of Non-Human Proteins/Peptides in Human Breast Milk by Mass Spectrometry

2020 ◽  
Vol 145 (2) ◽  
pp. AB163
Author(s):  
Kathleen Luskin ◽  
Sydney Leibel ◽  
Majid Ghassemian ◽  
Christina Chambers ◽  
Jessica Kitsen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Breast Milk ◽  
Human Breast Milk ◽  
Human Breast ◽  
Human Proteins

scholarly journals Quantification of non-persistent pesticides in small volumes of human breast milk with ultra-high performance liquid chromatography coupled to tandem mass-spectrometry

2020 ◽  
Author(s):  
Theresa L Pedersen ◽  
Jennifer T Smilowitz ◽  
Carl K Winter ◽  
Shiva Emami ◽  
Rebecca J Schmidt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Breast Milk ◽  
Tandem Mass Spectrometry ◽  
Solid Phase ◽  
Human Breast Milk ◽  
Tandem Mass ◽  
Human Breast ◽  
Low Volume ◽  
Volume Method

Existing methods for the analysis of pesticides in breast milk involves multiple extraction steps requiring large sample and solvent volumes, which can be a major obstacle in large epidemiologic studies. Here, we developed a simple, low-volume method for extracting organophosphates, pyrethroids, carbamates, atrazine and imidacloprid from 100-200 microliters of human breast milk. We tested microwave-assisted acid/base digestion and double solvent extraction with 2 or 20 mL of 2:1 (v/v) dichloromethane/hexane, with or without subsequent solid phase extraction (SPE) clean-up. Samples were analyzed by liquid chromatography tandem mass-spectrometry. Analyte recoveries and reproducibility were highest when 100-200 microliters milk were extracted with 2 mL of dichloromethane/hexane without subsequent SPE steps. Analysis of 79 breast milk samples using this method revealed the presence of carbamates, organophsphates, pyrethroids and imidacloprid at detection frequencies of 79-96%, 53-90%, 1-7% and 61%, respectively. This study provides a simple, low-volume method for measuring pesticides in human breast milk.

scholarly journals Analysis of Polybrominated Diphenyl Ethers and Lipid Composition in Human Breast Milk and Their Correlation with Infant Neurodevelopment

2021 ◽  
Vol 18 (21) ◽  
pp. 11501
Author(s):  
Ming-Hsien Tsai ◽  
How-Ran Chao ◽  
Wen-Li Hsu ◽  
Ching-Chung Tsai ◽  
Chu-Wen Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Breast Milk ◽  
Polybrominated Diphenyl Ethers ◽  
Food Additives ◽  
Public Health Issue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Diphenyl Ethers ◽  
Infant Neurodevelopment ◽  
Lipid Components

Breastfeeding is recommended over formula feeding, but human breast milk (HBM) composition varies and can be affected by food additives. Whether flame-retardant polybrominated diphenyl ethers (PBDEs) found in HBM interact with lipid components of HBM to impede infant neurodevelopment is a critical public health issue. Using lipidomic analysis, we examined the association of PBDEs in HBM and HBM lipid components with infant neurodevelopment. HBM samples (n = 100) were collected at the beginning stage of breastfeeding and analyzed for 30 PBDE congeners as well as a group of lipid components by using high-resolution gas chromatography, mass spectrometry, and liquid chromatography time-of-flight mass spectrometry. Infants were examined at 8 to 12 months of age by using the Bayley-III to assess neurodevelopment. A total of seven PBDEs, 35 lipids, and 27 fatty acids in HBM showed significant associations with Bayley-III scores. Multivariate analysis confirmed that these candidate PBDEs and lipid components were significant predictors of infant neurodevelopment. Eicosapentaenoic acid and docosapentaenoic acid in HBM showed no association with infant neurodevelopment in the general Taiwanese population. While certain PBDEs may play a role, our findings indicate that the lipid components of HBM are directly important for infant neurodevelopment.

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