scholarly journals Alternative Promoter use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain

2021 ◽  
Author(s):  
Toomas Jagomäe ◽  
Katyayani Singh ◽  
Mari-Anne Philips ◽  
Mohan Jayaram ◽  
Kadri Seppa ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Expression Patterns ◽  
Neuropsychiatric Disorders ◽  
Developing Brain ◽  
Alternative Promoter ◽  
Mrna Isoforms ◽  
Specific Expression ◽  
Embryonic Mouse

The members of the IgLON superfamily of cell adhesion molecules facilitate fundamental cellular communication during brain development, maintain functional brain circuitry, and are associated with several neuropsychiatric disorders. Usage of alternative promoter-specific 1a and 1b mRNA isoforms in Lsamp, Opcml, Ntm and the single promoter of Negr1 in the mouse and human brain has been previously described. To determine the precise spatiotemporal expression dynamics of Lsamp, Opcml, Ntm isoforms and Negr1, in the developing brain, we generated isoform-specific RNA probes and carried out in situ hybridization in the developing (embryonic, E10.5, 13.5, 17; post natal, P0) and adult mouse brains. We show that promoter-specific expression of IgLONs is established early during pallial development (at E10.5), where it remains throughout its differentiation through adulthood. In the diencephalon, midbrain and hindbrain, strong expression patterns are initiated a few days later and begin fading after birth, being only faintly expressed during adulthood. Thus, the expression of specific IgLONs in the developing brain may provide the means for regionally specific functionality as well as for specific regional vulnerabilities. The current study will therefore improve the understanding of how IgLON genes are implicated in the development of neuropsychiatric disorders.

scholarly journals Alternative Promoter Use Governs the Expression of IgLONCell Adhesion Moleculesin Histogenetic Fields of the Embryonic Mouse Brain

2021 ◽  
Vol 22 (13) ◽  
pp. 6955
Author(s):  
Toomas Jagomäe ◽  
Katyayani Singh ◽  
Mari-Anne Philips ◽  
Mohan Jayaram ◽  
Kadri Seppa ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Neuropsychiatric Disorders ◽  
Developing Brain ◽  
Alternative Promoter ◽  
Mrna Isoforms ◽  
Specific Expression ◽  
Embryonic Mouse ◽  
Spatiotemporal Expression ◽  
Rna Probes ◽  
Brain Circuitry

The members of the IgLON superfamily of cell adhesion molecules facilitate fundamental cellular communication during brain development, maintain functional brain circuitry, and are associated with several neuropsychiatric disorders such as depression, autism, schizophrenia, and intellectual disabilities. Usage of alternative promoter-specific 1a and 1b mRNA isoforms in Lsamp, Opcml, Ntm, and the single promoter of Negr1 in the mouse and human brain has been previously described. To determine the precise spatiotemporal expression dynamics of Lsamp, Opcml, Ntm isoforms, and Negr1, in the developing brain, we generated isoform-specific RNA probes and carried out in situ hybridization in the developing (embryonic, E10.5, E11.5, 13.5, 17; postnatal, P0) and adult mouse brains. We show that promoter-specific expression of IgLONs is established early during pallial development (at E10.5), where it remains throughout its differentiation through adulthood. In the diencephalon, midbrain, and hindbrain, strong expression patterns are initiated a few days later and begin fading after birth, being only faintly expressed during adulthood. Thus, the expression of specific IgLONs in the developing brain may provide the means for regionally specific functionality as well as for specific regional vulnerabilities. The current study will therefore improve the understanding of how IgLON genes are implicated in the development of neuropsychiatric disorders.

scholarly journals Stage- and segment-specific expression of cell-adhesion molecules N-CAM, A-CAM, and L-CAM in the kidney

1993 ◽  
Vol 44 (1) ◽  
pp. 147-158 ◽  
Author(s):  
Etienne J. Nouwen ◽  
Simonne Dauwe ◽  
Inge Van Der Biest ◽  
Marc E. De Broe
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Specific Expression

Adult and embryonic mouse neural cell adhesion molecules have different binding properties

Nature ◽  
1983 ◽  
Vol 304 (5924) ◽  
pp. 347-349 ◽  
Author(s):  
R. Sadoul ◽  
M. Hirn ◽  
H. Deagostini-Bazin ◽  
G. Rougon ◽  
C. Goridis
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Neural Cell ◽  
Binding Properties ◽  
Embryonic Mouse ◽  
Neural Cell Adhesion Molecules ◽  
Neural Cell Adhesion

scholarly journals Subtle Roles of Down Syndrome Cell Adhesion Molecules in Embryonic Forebrain Development and Neuronal Migration

2021 ◽  
Vol 8 ◽  
Author(s):  
Manuela D. Mitsogiannis ◽  
Anna Pancho ◽  
Tania Aerts ◽  
Sonja M. Sachse ◽  
Ria Vanlaer ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Neurodevelopmental Disorders ◽  
Neuronal Migration ◽  
Cell Adhesion Molecules ◽  
Cortical Development ◽  
Axon Growth ◽  
Embryonic Mouse ◽  
Forebrain Development

Down Syndrome (DS) Cell Adhesion Molecules (DSCAMs) are transmembrane proteins of the immunoglobulin superfamily. Human DSCAM is located within the DS critical region of chromosome 21 (duplicated in Down Syndrome patients), and mutations or copy-number variations of this gene have also been associated to Fragile X syndrome, intellectual disability, autism, and bipolar disorder. The DSCAM paralogue DSCAM-like 1 (DSCAML1) maps to chromosome 11q23, implicated in the development of Jacobsen and Tourette syndromes. Additionally, a spontaneous mouse DSCAM deletion leads to motor coordination defects and seizures. Previous research has revealed roles for DSCAMs in several neurodevelopmental processes, including synaptogenesis, dendritic self-avoidance, cell sorting, axon growth and branching. However, their functions in embryonic mammalian forebrain development have yet to be completely elucidated. In this study, we revealed highly dynamic spatiotemporal patterns of Dscam and Dscaml1 expression in definite cortical layers of the embryonic mouse brain, as well as in structures and ganglionic eminence-derived neural populations within the embryonic subpallium. However, an in-depth histological analysis of cortical development, ventral forebrain morphogenesis, cortical interneuron migration, and cortical-subcortical connectivity formation processes in Dscam and Dscaml1 knockout mice (Dscamdel17 and Dscaml1GT) at several embryonic stages indicated that constitutive loss of Dscam and Dscaml1 does not affect these developmental events in a significant manner. Given that several Dscam- and Dscaml1-linked neurodevelopmental disorders are associated to chromosomal region duplication events, we furthermore sought to examine the neurodevelopmental effects of Dscam and Dscaml1 gain of function (GOF). In vitro, ex vivo, and in vivo GOF negatively impacted neural migration processes important to cortical development, and affected the morphology of maturing neurons. Overall, these findings contribute to existing knowledge on the molecular etiology of human neurodevelopmental disorders by elucidating how dosage variations of genes encoding adhesive cues can disrupt cell-cell or cell-environment interactions crucial for neuronal migration.

scholarly journals MCAM: A Database to Accelerate the Identification of Functional Cell Adhesion Molecules

2008 ◽  
Vol 6 ◽  
pp. CIN.S341 ◽  
Author(s):  
Anguraj Sadanandam ◽  
Sudipendra Nath Pal ◽  
Joe Ziskovsky ◽  
Prathibha Hegde ◽  
Rakesh K. Singh
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Expression Patterns ◽  
Sequence Information ◽  
Web Based ◽  
Hematopoietic Stem ◽  
Tumor Tissues ◽  
Using Data ◽  
High Throughput Experiments

In the post-genomic era, computational identification of cell adhesion molecules (CAMs) becomes important in defining new targets for diagnosis and treatment of various diseases including cancer. Lack of a comprehensive CAM-specific database restricts our ability to identify and characterize novel CAMs. Therefore, we developed a comprehensive mammalian cell adhesion molecule (MCAM) database. The current version is an interactive Web-based database, which provides the resources needed to search mouse, human and rat-specific CAMs and their sequence information and characteristics such as gene functions and virtual gene expression patterns in normal and tumor tissues as well as cell lines. Moreover, the MCAM database can be used for various bioinformatics and biological analyses including identifying CAMs involved in cell-cell interactions and homing of lymphocytes, hematopoietic stem cells and malignant cells to specific organs using data from high-throughput experiments. Furthermore, the database can also be used for training and testing existing transmembrane (TM) topology prediction methods specifically for CAM sequences. The database is freely available online at http://www.app1.unmc.edu/mcam .

scholarly journals Single-cell RNAseq reveals cell adhesion molecule profiles in electrophysiologically defined neurons

2016 ◽  
Vol 113 (35) ◽  
pp. E5222-E5231 ◽  
Author(s):  
Csaba Földy ◽  
Spyros Darmanis ◽  
Jason Aoto ◽  
Robert C. Malenka ◽  
Stephen R. Quake ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Expression Profiles ◽  
Projection Neurons ◽  
Specific Expression ◽  
Synaptic Specificity ◽  
Genes Encoding ◽  
Cell Type Specific Expression

In brain, signaling mediated by cell adhesion molecules defines the identity and functional properties of synapses. The specificity of presynaptic and postsynaptic interactions that is presumably mediated by cell adhesion molecules suggests that there exists a logic that could explain neuronal connectivity at the molecular level. Despite its importance, however, the nature of such logic is poorly understood, and even basic parameters, such as the number, identity, and single-cell expression profiles of candidate synaptic cell adhesion molecules, are not known. Here, we devised a comprehensive list of genes involved in cell adhesion, and used single-cell RNA sequencing (RNAseq) to analyze their expression in electrophysiologically defined interneurons and projection neurons. We compared the cell type-specific expression of these genes with that of genes involved in transmembrane ion conductances (i.e., channels), exocytosis, and rho/rac signaling, which regulates the actin cytoskeleton. Using these data, we identified two independent, developmentally regulated networks of interacting genes encoding molecules involved in cell adhesion, exocytosis, and signal transduction. Our approach provides a framework for a presumed cell adhesion and signaling code in neurons, enables correlating electrophysiological with molecular properties of neurons, and suggests avenues toward understanding synaptic specificity.

Sign in / Sign up

Export Citation Format

Share Document