scholarly journals Analisis Fenotipik dan Genotipik pada Abnormalitas Klon-klon Kelapa Sawit (Elaeis guineensis Jacq.) Hasil Kultur Jaringan

2018 ◽  
Vol 22 (1) ◽  
pp. 1
Author(s):  
Endang Yuniastuti
Keyword(s):  
Molecular Weight ◽  
Tissue Culture ◽  
Genetic Distance ◽  
Oil Palm ◽  
Genetic Similarity ◽  
Elaeis Guineensis ◽  
Sds Page ◽  
Elaeis Guineensis Jacq ◽  
Molecular Weight Of Dna

<p>Research to analyze phenotype and genotype of oil palm clone developed from tissue culture which showed normal fruits and abnormal fruits through SDS-PAGE protein technique, and RAPD technique. Result indicated that protein with molecular weight of 100 kDa could differentiate genotype with normal fruit and abnormal fruit from all clones observed. Study on DNA through RAPD technique revealed primers which differentiated genotypes (male flowered genotype, normal fruited genotype, and abnormal fruited genotype) within clone and interclonal, i.e. OPC-07, OPN-16, and SC 10-56. Molecular weight of DNA on zymogram bands pattern were different on each clone.</p>Genetic similarity among clones and among genotypes were high, more than 85% indicated close genetic distance. Dendogram based on matrix of genetic similarity indicated grouping on each genotype.

scholarly journals GENETIC DISTANCE OF 47 ACCESSIONS OF OIL PALM (Elaeis guineensis Jacq.) GERMPLASM FROM CAMEROON BASED ON MORPHOLOGICAL CHARACTER

2019 ◽  
Vol 27 (1) ◽  
pp. 25-40
Author(s):  
Sujadi Sujadi ◽  
Tiara S Wandita ◽  
Nanang Supena ◽  
Yurna Yenni
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Distance ◽  
Palm Oil ◽  
Oil Palm ◽  
Alternative Energy ◽  
Elaeis Guineensis ◽  
Distance Analysis ◽  
Elaeis Guineensis Jacq ◽  
Genetic Distance Analysis

Oil palm (Elaeis guineensis Jacq.) is a plantation commodity that has an important role in various aspects of life in Indonesia, especially the domestic economy. This is supported by the increasing demand for world palm oil for food needs (edible oil), industrial (oleochemical), and alternative energy sources based on biodiesel. The development of the palm oil industry requires several efforts to achieve increased national productivity, one of which is the utilization of quality seeds supported by the availability of genetic resources (germplasm) that have a high level of genetic diversity. Efforts that can be made for the development of oil palm in Indonesia is through the characterization of oil palm intoduction from abroad. Considering this, further research on genetic distance analysis of palm oil accession introduced from Cameroon is based on morphological characters to produce parent elders that can produce heterosis properties. Research conducted at Seed Garden of Adolina PTPN IV and laboratory analysis of plant material of Palm Oil Research Center located on Jl. Brigjen Katamso No. 5, Medan, North Sumatera, which lasted from December 2017 to February 2018. Single observations were made on 47 accession palm oil from Cameroon planted on December 2010 and ten trees from PPKS 540 variety for compare. Data analysis used was a description of the plant to know the character of plant morphology as well as genetic distance analysis. Analysis of genetic distance using PCA analysis and cluster analysis. The results obtained are based on the results of PCA (Principal Component Analysis) reduce the observed character into six major components that have eigen value > 1 and able to explain the material diversity tested for 73.8%. Based on the cluster analysis obtained the genetic distance of 47 accessions of palm oil from Cameroon by 57%. It can be concluded that, when the genetic distance between accessions is further away, the larger the genetic variability between the observed characters. If the genetic diversity is wider the greater the chance for successful selection in increasing the desired gene frequency.

scholarly journals Deteksi Kestabilan Genetik Ramet Kelapa Sawit Hasil Kultur In Vitro Menggunakan SSR

2016 ◽  
Vol 44 (1) ◽  
pp. 83
Author(s):  
Yuni Fitri Cahyaningsih ◽  
Ni Made Armini Wiendi ◽  
Dan Nurita Toruan-Mathius
Keyword(s):  
Tissue Culture ◽  
Stability Analysis ◽  
Oil Palm ◽  
Genetic Stability ◽  
Genetic Similarity ◽  
Qualitative Data ◽  
Elaeis Guineensis ◽  
Group Method ◽  
Pair Group

ABSTRACT<br /><br />Commercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.<br /><br />Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture<br /><br />

Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

2012 ◽  
Vol 68 (2) ◽  
pp. 313-317 ◽  
Author(s):  
S. H. Habib ◽  
S.-E. Ooi ◽  
Ondřej Novák ◽  
Danuše Tarkowská ◽  
Jakub Rolčík ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Wild Type ◽  
Somaclonal Variant ◽  
Elaeis Guineensis Jacq

scholarly journals Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis

2008 ◽  
Vol 8 (1) ◽  
pp. 62 ◽  
Author(s):  
Eng-Ti L Low ◽  
Halimah Alias ◽  
Soo-Heong Boon ◽  
Elyana M Shariff ◽  
Chi-Yee A Tan ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

scholarly journals Effect of Plant Gowth Regulator on The Gowth of Zygotic Embryos in Three Types of Oil Palm Fruit (Elaeis guineensis Jacq.) in Tissue Culture

2020 ◽  
Vol 9 (2) ◽  
pp. 149-159
Author(s):  
Hadi Surahman ◽  
Gogoh Sulaksono ◽  
Zulhermana Sembiring ◽  
Astuti Kurnianingsih ◽  
Dwi Putro Priadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Embryo Culture ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryos ◽  
Palm Fruit ◽  
Randomized Design ◽  
Elaeis Guineensis Jacq ◽  
Positive Results ◽  
Germination Stage

Surahman H, Sulaksono G, Sembiring Z, Kurnianingsih A, Priadi DP, Asmono D. 2020. Effect of plant gowth regulator on the gowth of zygotic embryos in three types of oil palm fruit (Elaeis guineensis Jacq.) in tissue culture. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands 9(2): 149-159.Embryo culture is a widely used and effective way of overcoming the dormancy nature of seeds in oil palm.  At the germination stage, the influence of gowth regulators play an important role although in relatively lower concentrations in the germination media. This aim research determined the effect of adding various concentrations of gowth regulator on the three types of oil palm fruit based on the thickness of the shell. This research carried out at the Tissue Culture Laboratory of PT. Sampoerna Ago Tbk. from September 2018 to January 2019. The gowth regulator substances used in this study were NAA, BAP and GA3. The composition of the gowth regulators used were H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3, H2 = 0.05 mg/L NAA + 0.3 mg/L BAP + 0.1 mg/L GA3, H3 = 0.05 mg/L NAA + 0.5 mg/L BAP + 0.1 mg/L GA3 and H4 = No gowth regulator. While the types of oil palm fruit used were C1 = Dura, C2 = Tenera and C3 = Pisifera. This research used a factorial complete randomized design with three replications. The results showed that in general the use of gowth regulator substances H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3 and the type of dura fruit showed positive results based on viability, scoring, survival and height of plantlets when compared with other treatments.

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