Analisis Fenotipik dan Genotipik pada Abnormalitas Klon-klon Kelapa Sawit (Elaeis guineensis Jacq.) Hasil Kultur Jaringan

<p>Research to analyze phenotype and genotype of oil palm clone developed from tissue culture which showed normal fruits and abnormal fruits through SDS-PAGE protein technique, and RAPD technique. Result indicated that protein with molecular weight of 100 kDa could differentiate genotype with normal fruit and abnormal fruit from all clones observed. Study on DNA through RAPD technique revealed primers which differentiated genotypes (male flowered genotype, normal fruited genotype, and abnormal fruited genotype) within clone and interclonal, i.e. OPC-07, OPN-16, and SC 10-56. Molecular weight of DNA on zymogram bands pattern were different on each clone.</p>Genetic similarity among clones and among genotypes were high, more than 85% indicated close genetic distance. Dendogram based on matrix of genetic similarity indicated grouping on each genotype.

Key factors in FTIR spectroscopic analysis of DNA: the sampling technique, pretreatment temperature and sample concentration

Fourier transform infrared (FTIR) spectroscopy has been considered as a powerful tool for analysing the characteristics of deoxyribonucleic acid (DNA) regardless of physical states, sample amounts and the molecular weight of DNA.

Metals, Semiconductors, and Alloy Nanowires on DNA Scaffolds: Synthesis and Device Applications

In this presentation, we will demonstrate fast and electroless UV-photo activation techniques to synthesize electrically conductive metals (Pd), semiconductors (CdS), and alloy (Au-Ag) nanowires of diameter ∼10–180 nm on DNA. The electrical characterization showed that DNA nanowires are either exhibiting Ohmic or semiconducting behavior having low contact resistance with the electrodes. The nanowires are about 1–10 micrometers long and the length depends on the molecular weight of DNA. Highly selective deposition on DNA is obtained by specific complexation between the metal ions and DNA, followed by the growth of nanoclusters in DNA chain to form the corresponding nanowires. The eventual diameter of the nanowires obtained in our experiments is over 10 nm that is significantly larger than the ∼1–2 nm diameter of the double helix DNA. The DNA nanowires orchestrated with semiconducting Si nanowires, carbon nanotubes, or co-deposited with metal nanoparticles can potentially lead to avenues for making complex single electron devices and Schottky nanodevices.