Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

2010 ◽  
Vol 22 (3) ◽  
pp. 371-379 ◽  
Author(s):  
Yoko Yamanishi ◽  
◽  
Shinya Sakuma ◽  
Tomohiro Iyanagi ◽  
Fumihito Arai ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Zona Pellucida ◽  
Donor Cell ◽  
Embryonic Cell ◽  
Design Concept ◽  
Cell Manipulation ◽  
Electrical Field ◽  
Basic Functions ◽  
On Chip

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-in-one unified microfluidic chip was successfully designed and fabricated.

Maskless Gray Scale Lithography and its 3D Microfluidic Applications

2011 ◽  
Vol 23 (3) ◽  
pp. 426-433 ◽  
Author(s):  
Yoko Yamanishi ◽  
◽  
Takuma Nakano ◽  
Yu Sawada ◽  
Kazuyoshi Itoga ◽  
...  
Keyword(s):  
Cross Section ◽  
Mechanical Stimulation ◽  
Microfluidic Chip ◽  
Zona Pellucida ◽  
Three Dimensional ◽  
Cell Manipulation ◽  
The Novel ◽  
Gray Scale

This paper presents the novel three-dimensional fabrication using maskless exposure equipment and threedimensional (3D) microfluidic cell manipulation uses grayscale data to directly control the exposed photoresist height without using a mask. The 3D microchannel and microvalve were fabricated simply using lowcost exposure and height ranging from 0 to 200 µm. The 3D microvalve prevents liquid leakage when the membrane is closed – difficult to do using conventional 2D photolithography. We removed the oocyte zona pellucida passing through the 3D microchannel whose cross-section is gradually restricted along the path to provide mechanical stimulation omnidirectionally on the oocyte surface. The microfluidic chip may contribute to make high peeled-oocyte throughput effective without damaging the oocytes.

scholarly journals Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

2021 ◽  
Author(s):  
Yuha Koike ◽  
Shunnosuke Kodera ◽  
Yoshiyuki Yokoyama ◽  
Takeshi Hayakawa
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Light Source ◽  
Microfluidic Chip ◽  
Laser Light ◽  
Cell Manipulation ◽  
Potential Candidate ◽  
Response Characteristics ◽  
On Chip ◽  
Laser Light Source

Abstract A light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel actuators. However, the system used in the previous work did not allow the targeted cells to be manipulated in real time because the system used in the previous work could only irradiate preprogrammed patterned light. Moreover, when a large number of gel actuators are integrated into a chip, the Gaussian distribution of the laser light source results in the response characteristics of the gel actuators varying with the location of the actuator. In this work, we constructed a system that homogenized the intensity of the patterned light used for irradiation, allowing multiple gel actuators to be driven in parallel in real time. The intensity-homogenized patterned light improved the variations in the response characteristics of the gel actuators, and as a result, we succeeded in actuating gel actuators with various light patterns in real time.

scholarly journals 2A2-K08 All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation Based on Micro-Robotics

2009 ◽  
Vol 2009 (0) ◽  
pp. _2A2-K08_1-_2A2-K08_4
Author(s):  
Shinya Sakuma ◽  
Yoko Yamanishi ◽  
Fumihito Arai ◽  
Tatsuo Arai ◽  
Akiyuki Hasegawa ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Embryonic Cell ◽  
Cell Manipulation

Closed-loop feedback control of microfluidic cell manipulation via deep-learning integrated sensor networks

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Ningquan Wang ◽  
Ruxiu Liu ◽  
Norh Asmare ◽  
Chia-Heng Chu ◽  
Ozgun Civelekoglu ◽  
...  
Keyword(s):  
Deep Learning ◽  
Sensor Networks ◽  
Feedback Control ◽  
Closed Loop ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Integrated Sensor ◽  
Loop Feedback ◽  
Closed Loop Feedback ◽  
On Chip

An adaptive microfluidic system changing its operational state in real-time based on cell measurements through an on-chip electrical sensor network.

scholarly journals Temperature-controlled MPa-pressure ultrasonic cell manipulation in a microfluidic chip

Lab on a Chip ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 3341-3349 ◽  
Author(s):  
Mathias Ohlin ◽  
Ida Iranmanesh ◽  
Athanasia E. Christakou ◽  
Martin Wiklund
Keyword(s):  
Lung Cancer ◽  
High Pressure ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Acoustic Streaming ◽  
Cell Manipulation ◽  
Wave Trapping ◽  
Ultrasonic Standing Wave ◽  
Temperature Controlled

We study the effect of 1 MPa-pressure ultrasonic-standing-wave trapping of cells during one hour in a fully temperature- and acoustic streaming-controlled microfluidic chip, and conclude that the viability of lung cancer cells are not affected by this high-pressure, long-term acoustophoresis treatment.

On-chip cavity-enhanced absorption spectroscopy using a white light-emitting diode and polymer mirrors

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 711-717 ◽  
Author(s):  
Cathy M. Rushworth ◽  
Gareth Jones ◽  
Martin Fischlechner ◽  
Emma Walton ◽  
Hywel Morgan
Keyword(s):  
Absorption Spectroscopy ◽  
White Light ◽  
Microfluidic Chip ◽  
Path Length ◽  
Light Emitting Diode ◽  
Absorption Path Length ◽  
Light Emitting ◽  
Enhanced Absorption ◽  
Cavity Enhanced Absorption Spectroscopy ◽  
On Chip

We have integrated disposable polymer mirrors within a microfluidic chip to form a multi-pass cell, which increases the absorption path length by a maximum of 28 times, providing micromolar detection limits in a probed volume of 10 nL.

Multistage Isoelectric Focusing: A Novel On-Chip Bio-Separation Technique

2005 ◽  
Author(s):  
Prashanta Dutta ◽  
Keisuke Horiuchi ◽  
Huanchun Cui ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Fluorescent Protein ◽  
Ph Gradient ◽  
Resolving Power ◽  
Protein Species ◽  
Second Stage ◽  
On Chip ◽  
Bonding Technique

This experimental study reports a method to increase the resolving power of isoelectric focusing (IEF) on a polymeric microfluidic chip. Microfluidic chip is formed on poly-di-methyl siloxane (PDMS) using soft lithography and multilayer bonding technique. In this novel bioseparation technique, IEF is staged by first focusing protein species in a straight channel using broad-range ampholytes and then refocusing segments of that first channel into secondary channels that branch out from the first one. Experiments demonstrated that three fluorescent protein species within a segment of pH gradient in the first stage were refocused in the second stage with much higher resolution in a shallower pH gradient. A serially performed two-stage IEF was completed in less than 25 minutes under particularly small electric field strength up to 100 V/cm.

From CAD to microfluidic chip within one day: rapid prototyping of lab-on-chip cartridges using generic polymer parts

2020 ◽  
Vol 30 (11) ◽  
pp. 115012 ◽  
Author(s):  
Daniel Podbiel ◽  
Lorenz Boecking ◽  
Hannah Bott ◽  
Julian Kassel ◽  
Daniel Czurratis ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Microfluidic Chip ◽  
Lab On Chip ◽  
On Chip
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