scholarly journals Pengaruh Penggunaan Tepung Onggok Terfermentasi Dengan Trichoderma viride Sebagai Pakan Alternatif Terhadap Konsumsi Pakan dan Pertambahan Bobot Badan Ayam Kampung Super

2019 ◽  
Vol 7 (2) ◽  
pp. 128-134
Author(s):  
Anang Widigdyo ◽  
Agustina Widyaworo
Keyword(s):  
Trichoderma Viride

Penelitian ini bertujuan untuk mengetahui level penggunaan tepung onggok terfermentasi sebagai pakan alternative pengganti Bekatul dalam meningkatkan penampilan produksi ayam kampung super yang meliputi konsumsi pakan dan pertambahan bobot badan sehingga dapat meningkatkan pendapatan masyarakat dalam usaha peternakan ayam kampung super. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan teknik observasi. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dan 6 kali ulangan. Perlakuan yang digunakan pada penelitian ini P0 merupakan perlakuan tanpa penambahan Tepung Onggok Terfermentasi (TOT), P1 penambahan tepung onggok sebagai subtitusi bekatul 25 % Tepung Onggok Terfermentasi (TOT) dan 75 % Bekatul dalam ransum untuk mengganti penggunaan Bekatul, P2,penambahan TOT 50 % + 50 % Bekatul dalam ransum, P3, penambahan TOT 75% + 25% Bekatul dalam ransum. Dalam penelitian ini menggunakan ternak ayam kampung super umur 28 hari sebanyak 120 ekor dan terbagi 5 ekor di setiap ulangan. Kandang yang digunakan adalah kandang koloni dengan ukuran 100 x 70 x 80 cm di setiap perlakuan dan ulangan. Hasil data penelitian menunjukkan bahwa penggunaan TOT sebagai pengganti bekatul dalam ransum pakan ayam kampung super memberikan pengaruh yang nyata (P<0,05) terhadap nilai konsumsi pakan ayam kampung super. Hasil data penelitian penggunaan TOT sebagai pengganti bekatul dalam ransum pakan tidak memberikan pengaruh yang nyata terhadap Pertambahan Bobot Badan (PBB) ayam kampung super .

Fungicidal and Bactericidal Activity of the Alkyl-Substituted Guanidine-Containing Oligomers

2020 ◽  
Vol 82 (6) ◽  
pp. 54-63
Author(s):  
M.Ya. Vortman ◽  
◽  
Yu.B. Pysmenna ◽  
A.I. Chuenko ◽  
D.R. Abdulina ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Trichoderma Viride ◽  
Rhodococcus Erythropolis ◽  
Biological Action ◽  
Molar Ratio ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Alkyl Bromides ◽  
Base Form ◽  
Two Stages

Biocides are widely used in medicine and various industries to protect against a number of harmful microorganisms. Organic quaternary ammonium and guanidine-containing compounds, the biological action of which is based on membrane-toxic properties, are used as bactericidal preparations. The aim of this work was to study the bactericidal and fungicidal activities of the synthesized oligomeric alkylsubstituted guanidinium bromides with different radicals -C3H7, -C7H15, -C10H21, against different isolates of heterotrophic bacteria and microscopic fungi. Methods. The synthesis of alkyl-substituted guanidiniumcontaining oligomers was performed in two stages. In the first stage, alkyl-substituted guanidine was obtained by the reaction of guanidine, previously converted by alkali from the salt form to the base form by the base and alkyl bromides (Alk=-C3H7 (propyl), -C7H15 (heptyl), -C10H21 (decyl)) in methanol at a temperature of 50°C and a molar ratio of 1:1. The second carried out the reaction between aromatic oligoepoxide DER-331 and alkyl-substituted guanidine in methanol at a temperature of 50°C for 2–3 hours and a molar ratio of 1:2. Bacteria were grown on meat-peptone agar for 48 hours at a temperature of 28±2°С. Test cultures of micromycetes were cultured on agar beer wort (6°B), incubated for 14 days in a thermostat at a temperature of 28±2°C. Antimicrobial activity of newly synthesized alkyl-substituted guanidinium-containing oligomers was determined by standard disco-diffusion method (method of disks on agar) and fungicidal activity was determined by the method of holes in agar. Results. Oligomeric alkylsubstituted guanidinium bromides with different radicals composed -C3H7, -C7H15, -C10H21- synthesized by the reaction of guanidine alkyl bromides with aromatic oligoepoxydes. It was found that alkyl-substituted guanidinium-containing oligomers at a concentration of 1–3% inhibited the growth of Escherichia coli 475, Pseudomonas aeruginosa 465, Klebsiella pneumonia 479, Pseudomonas pseudoalcaligenes 109, Staphylococcus aureus 451, E. faecalis 422, Rhodococcus erythropolis 102, Bacillus subtilis 138 and most of the studied micromycetes – Aureobasidium pullulans F-41430, Paecilomyces variotii F-41432, Penicillium funiculosum F-41435, Penicillium ochrochloron F-41431, Scopulariopsis brevicaulis F-41434, Trichoderma viride F-41437, Candida albicans F-41441, Aspergillus flavus F-41442, Aspergillus niger F-41448, Penicillium sp. F-41447. Conclusions. Antimicrobial and fungicidal properties significantly depend on the length of the alkyl radical, with increasing of its length the diameter of the zone of bacterial and micromycetes growth retardation increases.10.15407/microbiolj82.06.054

scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

scholarly journals Structural basis of enzyme activity regulation by the propeptide of l-lysine α-oxidase precursor from Trichoderma viride

2021 ◽  
Vol 5 ◽  
pp. 100044
Author(s):  
Masaki Kitagawa ◽  
Nanako Ito ◽  
Yuya Matsumoto ◽  
Masaya Saito ◽  
Takashi Tamura ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Trichoderma Viride ◽  
Structural Basis ◽  
Activity Regulation
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