scholarly journals Penyebaran Polen Berdasarkan Analisis SSR Membuktikan Penyerbukan

Buletin Palma ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 77 ◽  
Author(s):  
SITI HALIMAH LAREKENG ◽  
ISMAIL MASKROMO ◽  
AGUS PURWITO ◽  
NURHAYATI ANSHORI MATTJIK ◽  
SUDARSONO SUDARSONO
Keyword(s):  
Ssr Marker ◽  
Cocos Nucifera ◽  
Pollen Dispersal ◽  
Paternity Analysis ◽  
Font Size ◽  
Cross Pollination ◽  
Ssr Analysis ◽  
Self Pollination ◽  
Marker Loci ◽  
Cocos Nucifera L

<p><span style="font-size: medium;">ABSTRAK </span></p><p>Analisis paternitas digunakan untuk mengetahui pola penyebaran serbuk sari pada kelapa (Cocos nucifera L.) tipe Dalam Kalianda. Tujuan penelitian ini adalah untuk (1) mengevaluasi pola penyebaran serbuk sari dan menentukan kisaran  jarak penyebaran serbuk sari pada kelapa tipe Dalam Kalianda, (2) menentukan persentase penyerbukan silang  (outcrossing) dan penyerbukan sendiri (selfing) yang terjadi pada kelapa tipe Dalam Kalianda, dan (3) menentukan  frekuensi pola penyerbukan silang antara kelapa tipe Dalam normal (N) dengan kelapa Dalam Kopyor (K), KxN dan KxK yang terjadi pada populasi campuran antara kelapa tipe Dalam Kopyor dan kelapa Dalam normal Kalianda.  Populasi yang digunakan terdiri atas 60 pohon kelapa tipe Dalam dewasa, 21 pohon merupakan kelapa tipe Dalam berbuah normal (homozigot KK) dan 39 merupakan pohon kelapa tipe Dalam Kopyor (heterosigot Kk). Empat belas  pohon (5 pohon KK dan 9 pohon Kk) digunakan sebagai tetua betina. Sebanyak 49 progeni dipanen dari 15 induk  terpilih dan dikecambahkan untuk sumber DNA dalam analisis paternitas. Enam lokus marka SSR polimorfik, yaitu  CnCir_B12,  CnCir_86,  CnCir_87,  CnCir_56,  CnZ_51,  CnZ_18  dan  empat  lokus  marka  SNAP  polimorfik,  yaitu  CnSUS1#14,CnSUS1#3, CnWRKY6#3 dan CnWRKY19#1 digunakan untuk menentukan genotipe seluruh progeni, seluruh kandidat tetua jantan, dan semua tetua betina yang digunakan. Hasil penelitian menunjukkan bahwa  serbuk sari kelapa tipe Dalam Kalianda menyebar dengan jarak terjauh 63 m. Jarak penyebaran serbuk sari terbanyak pada jarak 40-50 m, dengan frekuensi sebesar 13 kejadian polinasi (27%). Dari 47 progeni yang dievaluasi, hanya satu (2%) progeni yang berasal dari penyerbukan sendiri (self pollination) dan 48 (98%) berasal dari penyerbukan silang. Dari  progeni hasil penyerbukan silang, 24 (49,0%) progeni teridentifikasi sebagai hasil persilangan antara induk dan tetua  jantan kelapa tipe Dalam kopyor heterosigot Kk, 11 (22,4%) sebagai hasil persilangan antara induk kelapa tipe Dalam  Kopyor heterosigot Kk dan normal homosigot KK, 10 (20,5%) sebagai hasil persilangan antara induk kelapa tipe Dalam normal homosigot KK dan Kopyor heterosigot Kk, serta 3 (6,1%) sebagai hasil persilangan antara induk dan tetua jantan tipe Dalam normal homosigot KK.</p><p>Kata kunci : Kelapa Dalam Kopyor, kelapa Kopyor Kalianda, tingkat penyerbukan sendiri, tingkat penyerbukan silang.</p><p> </p><p><strong>Pollen Dispersal Based on SSR Analysis Proves Kalianda to Kopyor Coconut Pollinations</strong></p><p><span style="font-size: medium;">ABSTRACT </span></p><p>Paternity analysis was applied to determine the pattern of pollen spread among Kalianda Tall coconut (Cocos nucifera L.) in Kalianda, Lampung. The objectives of this research are to (1) evaluate patterns of pollen dispersal and ranges of pollen  spread, (2) determine percentage of outcrossing or selfing rates, and (3) determine the frequency of cross pollination  among normal (N) to kopyor (K), KxN and KxK in the mix population of Kalianda Tall coconut at Kalianda, Lampung. The population used in this study was 60 palms, consisted of 21 Kalianda Tall Normal coconuts (homozygous KK) and 39 Kalianda Tall Kopyor coconuts (Heterozygous Kk). Fourteen palms out of those were selected as female parents. Progeny arrays (49 nuts) were harvested from 15 female parents and they were germinated. The DNA was isolated from  young leaf of all adult palms and germinated coconut seedlings and they were used in paternity analysis. Six  polymorphic SSR marker loci used were CnCir_B12, CnCir_86, CnCir_87, CnCir_56, CnZ_51, CnZ_18 and the four  polymorphic SNAP markers used were CnSUS1#14, CnSUS1#3, CnWRKY6#1 and CnWRKY19#3. The markers were used to genotype all the progenies, the potential male and the female parents. Results of the experiment indicated pollen of Kalianda Tall Kopyor coconut farthest disperse was 63 m. Distance of the mostpollen dispersal was between 40-50  m,with the frequency of 13 pollination events (27%). Among the evaluated progenies, only one (2%) comes from self  pollination event and 48 (98%) comes from cross pollination. Results of the progeny evaluation also indicated 24  progenies (49.0%) are results of outcrossing among Kalianda Tall kopyor heterozygous Kk parents, 11 progenies (22.4%)  are outcrossing among kopyor heterozygous Kk female and normal homozygous KK male parents, 10 progenies (20.5%) are outcrossing among normal homozygous KK female and kopyor heterozygous Kk male parents, and 3 progenies (6.1%) are outcrossing among normal homozygous KK female and male parents.</p>Keywords : Tall kopyor coconut, Kalianda Kopyor coconut, self polination, cross pollination rate.

scholarly journals Pollen Dispersal and Pollination Patterns Studies in Pati Kopyor Coconut using Molecular Markers

CORD ◽  
2015 ◽  
Vol 31 (1) ◽  
pp. 15
Author(s):  
S. Sudarsono
Keyword(s):  
Cocos Nucifera ◽  
Pollen Dispersal ◽  
Female Parent ◽  
Parentage Analysis ◽  
Specific Direction ◽  
Pollen Donor ◽  
Marker Loci ◽  
Version 2.0 ◽  
Cocos Nucifera L ◽  
Fruit Harvest

Parentage analysis has been used to evaluate pollen dispersal in Kopyor coconut (Cocos nucifera L.). Investigations were undertaken to elucidate (i) the dispersal of pollen, (ii) the rate of self and out-crossing pollination, and (iii) the distance of pollen travel in Pati kopyor coconut population. The finding of this activities should be beneficial to kopyor coconut farmers to increase their kopyor fruit harvest and to support breeding of this unique coconut mutant. As many as 84 progenies were harvested from 15 female parents. As many as 95 adults coconut provenances surrounding the female parents were analyses as the potential male parents for the progenies. The adult coconut palms were mapped according to their GPS position. All samples were genotyped using six SSR and four SNAP marker loci. Parentage analysis was done using CERVUS version 2.0 software. Results of the analysis indicated that evaluated markers were effective for assigning candidate male parents to all evaluated seedlings. There is no specific direction of donated pollen movement from assigned donor parents to the female ones. The donated pollens could come from assigned male parents in any directions relative to the female parent positions. Cross pollination occured in as many as 82.1% of the progenies analyzed. Outcrossing among tall by tall (TxT), dwarf by dwarf (DxD), hybrid by hybrid (HxH), TxD, DxT, TxH, DxH, and HxD were observed. Self-pollination (TxT and DxD) occurred in as many as 17.9% of the progenies. The dwarf coconut was not always self pollinated. The presence of DxD, TxD, and HxD outcrossing was also observed. The donated pollens could come from pollen donor in a range of at least 0-58 m apart from the evaluated female recipients. Therefore, in addition to the wind, insect pollinators may have played an important role in Kopyor coconut pollination.

scholarly journals Doing the twist: a test of Darwin's cross-pollination hypothesis for pollinarium reconfiguration

2005 ◽  
Vol 2 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Craig I Peter ◽  
Steven D Johnson
Keyword(s):  
Inbreeding Depression ◽  
Mating Success ◽  
Positive Relationship ◽  
Pollen Dispersal ◽  
Strong Support ◽  
Cross Pollination ◽  
Self Pollination ◽  
Strong Positive Relationship ◽  
Mating Opportunities

Mating success in plants depends largely on the efficiency of pollen dispersal. For hermaphrodite plants, self-pollination, either within or among flowers, can reduce mating opportunities because of pollen and ovule discounting and inbreeding depression. Self-pollination may be particularly detrimental in plants such as orchids and asclepiads that package each flower's pollen into one or more pollinia which, together with accessory structures, comprise a pollinarium. Darwin proposed that physical reconfiguration of pollinaria serves as a mechanism for reducing the likelihood of self-pollination. To be effective, the time taken for pollinarium reconfiguration would need to exceed that spent by a pollinator on a plant. We investigated pollinarium reconfiguration (including pollinarium bending, pollinium shrinking and anther cap retention) in 19 species and found a strong positive relationship between reconfiguration time and the duration of pollinator visits. Reconfiguration times were also consistently longer than pollinator visit times. These results provide strong support for Darwin's idea that this mechanism promotes cross-pollination.

scholarly journals Development of Self-pollinating and Early-bearing Philippine Makapuno (Cocos nucifera L.) Hybrids

2012 ◽  
pp. 63-74
Author(s):  
Tessie Nuñez ◽  
Edwin Ocoy
Keyword(s):  
Lauric Acid ◽  
Cocos Nucifera ◽  
The Philippines ◽  
The Self ◽  
F1 Hybrids ◽  
Seed Industry ◽  
Self Pollination ◽  
F2 Hybrids ◽  
Cocos Nucifera L ◽  
High Degree

The makapuno-bearing character found only in the cross-pollinated Laguna Tall coconut variety and the self-pollinating character of dwarf coconuts were combined into new makapuno genotypes through hybridization to limit cross pollination which is a problem among tall makapuno palms. F1 hybrids between Coconiño (CÑO) and tall Makapuno (TMAC) and Tacunan (TAC) x TMAC were early-bearing with high intraspadix overlapping percentages of 85.7% and 70.90, respectively. Mean makapuno yield was 26.8% in CÑO x TMAC and 28.2% in TAC x TMAC indicating the presence of high degree of self-pollination and the dominance of the gene for the trait. ln vitro grown homozygous F2 makapuno palms started flowering at 24 months old. F2 CÑO x TMAC (VMAC 1) had a mean yield of 100% makapuno, 49 to 132 nuts/palm/year and nuts weighing 332 to 750g/dehusked nut. F2 MRD x TMAC (VMAC 2) yielded a mean of 97% makapuno, 63 to 163 nuts/palm/year and nuts weighing 288 to 1,180g each. F2 TAC x TMAC (VMAC5) had 95% makapuno, nuts weighing 736g to 1,975g/ dehusked nut. High makapuno yield of the F2 hybrids affirmed the dominance of the gene for self-pollination in coconut. Ten month-old nuts of the F2s had at least 18 mm thick meat and Lauric acid (C12) content ranging from 47.10% to 48.60%. The three F2 hybrids were registered with the National Seed Industry Council (NSIC) in 2008 as the first homozygous makapuno hybrids in the Philippines.

Construction of a linkage map of the Rennell Island Tall coconut type (Cocos nucifera L.) and QTL analysis for yield characters

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 962-970 ◽  
Author(s):  
P Lebrun ◽  
L Baudouin ◽  
R Bourdeix ◽  
J Louis Konan ◽  
J HA Barker ◽  
...  
Keyword(s):  
Linkage Map ◽  
Qtl Analysis ◽  
Dna Markers ◽  
Marker Assisted Selection ◽  
Ssr Marker ◽  
Cocos Nucifera ◽  
Genome Length ◽  
Linkage Groups ◽  
Consecutive Sampling ◽  
Cocos Nucifera L

AFLP and SSR DNA markers were used to construct a linkage map in the coconut (Cocos nucifera L.; 2n = 32) type Rennell Island Tall (RIT). A total of 227 markers were arranged into 16 linkage groups. The total genome length corresponded to 1971 cM for the RIT map, with 5–23 markers per linkage group. QTL analysis for yield characters in two consecutive sampling periods identified nine loci. Three and two QTLs were detected for number of bunches and one and three QTLs for number of nuts. The correlation of trait values between characters and evaluation periods is partially reflected in identical QTLs. The QTLs represent characters that are important in coconut breeding. The cosegregation of markers with these QTLs provides an opportunity for marker-assisted selection in coconut breeding programmes.Key words: coconut, QTL, AFLP, SSR, marker-assisted selection (MAS).

Growth of Dracaena marginata and Spathiphyllum ‘Petite’ in Sphagnum Peat- and Coconut Coir Dust-based Growing Media

1999 ◽  
Vol 17 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Robert H. Stamps ◽  
Michael R. Evans
Keyword(s):  
Growth Parameters ◽  
Cocos Nucifera ◽  
Greenhouse Production ◽  
Growing Media ◽  
Sphagnum Peat ◽  
Affected Plant ◽  
Coir Dust ◽  
Media Component ◽  
The Media ◽  
Cocos Nucifera L

Abstract A comparison was made of Canadian sphagnum peat (SP) and Philippine coconut (Cocos nucifera L.) coir dust (CD) as growing media components for greenhouse production of Dracaena marginata Bak. and Spathiphyllum Schott ‘Petite’. Three soilless foliage plant growing mixes (Cornell, Hybrid, University of Florida #2 [UF-2]) were prepared using either SP or CD and pine bark (PB), vermiculite (V), and/or perlite (P) in the following ratios (% by vol): Cornell = 50 CD or SP:25 V:25 P, Hybrid = 40 CD or SP:30 V:30 PB, UF-2 = 50 CD or SP: 50 PB. Dracaena root growth was not affected by treatments but there were significant mix × media component interactions that affected plant top growth parameters. In general, the growth and quality of D. marginata were reduced by using CD in Cornell, had no effect in Hybrid, and increased in UF-2. S. ‘Petite’ grew equally well in all growing mixes regardless of whether CD or SP was used; however, plants grew more in Cornell and Hybrid than in UF-2. S. ‘Petite’ roots, which were infested with Cylindrocladium spathiphylli, had higher grades when grown in CD than when the media contained SP.

Diversity in Mohachao Narel, a Sweet Endosperm Coconut (Cocos nucifera L.) Population from Maharashtra, India

2013 ◽  
Vol 36 (3) ◽  
pp. 319-330 ◽  
Author(s):  
K. Samsudeen ◽  
M. K. Rajesh ◽  
D. D. Nagwaker ◽  
Raghavan Reshmi ◽  
P. Ajith Kumar ◽  
...  
Keyword(s):  
Cocos Nucifera ◽  
Cocos Nucifera L

Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olugbemi T. Olaniyan ◽  
Olakunle A. Ojewale ◽  
Ayobami Dare ◽  
Olufemi Adebayo ◽  
Joseph E. Enyojo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Cocos Nucifera ◽  
Reproductive Toxicity ◽  
Positive Control ◽  
Negative Control ◽  
Male Wistar Rats ◽  
Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Sign in / Sign up

Export Citation Format

Share Document