Elevated Temperature Induced Adaptive Responses of Two Lupine Species at Early Seedling Phase

This study aimed to investigate the impact of climate warming on hormonal traits of invasive and non-invasive plants at the early developmental stage. Two different lupine species—invasive Lupinus polyphyllus Lindl. and non-invasive Lupinus luteus L.—were used in this study. Plants were grown in climate chambers under optimal (25 °C) and simulated climate warming conditions (30 °C). The content of phytohormone indole-3-acetic acid (IAA), ethylene production and the adaptive growth of both species were studied in four-day-old seedlings. A higher content of total IAA, especially of IAA-amides and transportable IAA, as well as higher ethylene emission, was determined to be characteristic for invasive lupine both under optimal and simulated warming conditions. It should be noted that IAA-L-alanine was detected entirely in the invasive plants under both growth temperatures. Further, the ethylene emission values increased significantly in invasive lupine hypocotyls under 30 °C. Invasive plants showed plasticity in their response by reducing growth in a timely manner and adapting to the rise in temperature. Based on the data of the current study, it can be suggested that the invasiveness of both species may be altered under climate warming conditions.

Foliar Application of Polyamines Modulates Winter Oilseed Rape Responses to Increasing Cold

Cold stress is one of the most common abiotic stresses experienced by plants and is caused by low temperature extremes and variations. Polyamines (PAs) have been reported to contribute in abiotic stress defense processes in plants. The present study investigates the survival and responses of PA-treated non-acclimated (N) and acclimated (A) winter oilseed rape to increasing cold conditions. The study was conducted under controlled conditions. Seedlings were foliarly sprayed with spermidine (Spd), spermine (Spm), and putrescine (Put) solutions (1 mM) and exposed to four days of cold acclimation (4 °C) and two days of increasing cold (from −1 to −3 °C). Two cultivars with different cold tolerance were used in this study. The recorded traits included the percentage of survival, H+-ATPase activity, proline accumulation, and ethylene emission. Exogenous PA application improved cold resistance, maintained the activity of plasma membrane H+-ATPase, increased content of free proline, and delayed stimulation of ethylene emission under increasing cold. The results of the current study on winter oilseed rape revealed that foliar application of PAs may activate a defensive response (act as elicitor to trigger physiological processes), which may compensate the negative impact of cold stress. Thus, cold tolerance of winter oilseed rape can be enhanced by PA treatment.

Sage Essential Oil Improves the Effectiveness of Aloe vera Gel on Postharvest Quality of Tomato Fruit

Edible coating is extensively applied for fresh produce preservation. In the present study, Aloe vera gel (AV) alone or combined with sage essential oil (EO; 0.1% or 0.5%) was used as fruit edible coating in tomatoes. Following treatments, tomatoes were stored at 11 °C and 90% relative humidity (RH) up to 14 days and analyzed for quality maintenance compared with uncoated fruits (control). Results showed that both AV and EO decreased decay symptoms with more pronounced effects on low EO concentration (i.e., 0.1%). The application of 10% of AV coating decreased fruit ethylene emission while EO (0.1% and 0.5%) application increased ethylene emission and respiration rates. AV coating decreased acidity, β-carotene, lycopene content and maintained fruit firmness. High levels of EO (0.5%) increased weight loss but decreased fruits’ redness, chroma, total soluble solids, acidity, β-carotene and lycopene content. Total phenolic content and antioxidants maintained or increased during storage when treated with AV and/or EO. Low levels of EO (0.1%) maintained or improved fruit quality characteristics during storage, and this was mirrored by the sensory evaluation preference (including the AV application) and quality related attributes.

Newly Established Method for Analyzing the Natural Ethylene Indicators Dissolved in Water Immersed Plant Using Acidified Potassium Manganate

Ethylene is a plant growth regulator (PGR) that mainly influences on growth and development of plant and their agricultural productivity. Ethylene producing from plant tissue is a physiological indicator which refers to the maturity, senescence, or stress of plants. Therefore, a monitoring level of ethylene in plant tissue is necessary for research and commercial processes. Current method measuring gaseous ethylene is costly and take a long time. This research established a new method to quantify ethylene gas in water immersed plant by using an oxidation reaction between oxidizing agent (KMnO4) and alkene functional group of ethylene under acidic solutions. Then, the leftover KMnO4 in solution was quantitatively evaluated by spectrophotometry method. An absorbance at the wavelength 525 nm were compared to the standard curve. The purple intensity of KMnO4 inversely relates to the concentration of ethylene. Water immersions of leaf and flower samples which have different in dissolved ethylene were analyzed under this method. The result showed the difference in ethylene emission between plant varieties (normal tobacco and less-ethylene emission tobacco), plant organs (leaf and flowers) and also organ senescent levels. The result indicates that the concentration of ethylene in water is concordant with the physiology of tobacco leaf and flower. Therefore, the ethylene level in water immersed plant sample can be estimated by this established method.