scholarly journals PENGARUH PENAMBAHAN STARTER MIKROBA SERTA PEMERASAN PULP TERHADAP KONDISI FERMENTASI DAN MUTU BIJI KAKAO (Theobroma cacao L.)

2018 ◽  
Vol 13 (3) ◽  
pp. 156
Author(s):  
S. Joni Munarso ◽  
Kun Tanti Dewandari ◽  
Zahra Haifa
Keyword(s):  
Acetic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Concentration ◽  
Theobroma Cacao ◽  
Starter Culture ◽  
Acetic Acid Concentration ◽  
Acetobacter Aceti ◽  
Cocoa Beans ◽  
Theobroma Cacao L

<p>Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh penambahan starter mikroba (<em>Acetobacter aceti, Lactobacillus plantarum dan Saccharomyces cereviceae</em>) serta pemerasan pulp terhadap fermentasi dan mutu biji kakao. Penelitian menggunakan metode Rancangan Acak Lengkap (RAL) pola faktorial 3x5 dengan dua kali ulangan. Faktor pertama adalah fermentasi yaitu fermentasi biji kakao secara spontan (F1), Fermentasi biji kakao dengan penambahan <em>A. aceti, L. plantarum dan S. cereviciae</em> (F2), Fermentasi biji kakao dengan perlakuan pemerasan pulp serta penambahan <em>A. aceti, L. plantarum dan S. cereviciae</em> (F3). Sedangkan faktor kedua adalah lama fermentasi (H1, H2, H3, H4 dan H5). Hasil penelitian menunjukkan bahwa penambahan starter meningkatkan konsentrasi etanol pada saat fermentasi dan meningkatkan kadar asam asetat, tetapi menurunkan konsentrasi asam oksalat pada biji kakao. Penambahan starter disertai pemerasan pulp menghasilkan biji kakao dengan kadar asam asetat sebesar 0,47%, sedangkan biji kakao tanpa pemerasan kadar asam asetat 0,49%. Penambahan starter disertai pemerasan pulp menghasilkan mutu biji kakao terbaik dengan karakteristik sebagai berikut: skor nilai uji belah tertinggi (379 dari 400), mutu fisik (Golongan mutu A) serta memenuhi persyaratan mutu SNI 2008 No. 2323 tentang biji kakao dengan rasio jumlah per berat biji sebanyak 88 biji/100g; nilai pH 4,93; kadar asam asetat 0,47%, kadar lemak 34,90%, kadar air 4,47%, kadar serat kasar 3,66% dan kadar abu 4,82% dengan waktu fermentasi selama 5 hari.</p><p align="center"><strong>English Version Abstract</strong></p><p align="center"><strong><strong>Effect of Starter Culture Addition and Depulping on The Fermentation and Quality of Cocoa Beans (Theobroma cacao L.)</strong></strong></p><p>S Joni Munarso, Kun Tanti Dewandari, and Zahra Haifa. 2016. Effect of Starter Culture Addition and Depulping on The Fermentation and Quality of Cocoa Beans (<em>Theobroma cacao L</em>.). The aimed of this study was to investigate the effect of starter culture addition (<em>Acetobacter aceti, Lactobacillus plantarum, and Saccharomyces cerevisiae</em>) with depulping on the fermentation and quality of cocoa beans. The experimental design of this study was conducted using a 3×5 factorial Completely Randomized Design (CRD) with duplicate replication. The first factor was fermentation condition included spontaneously fermented cocoa beans (F1), fermentation of cocoa beans with the addition of <em>A. aceti, L. plantarum and S. cereviciae </em>(F2), Fermented cocoa beans with depulping and addition of <em>A. aceti, L. plantarum and S. Cereviciae</em> (F3). The second factor was time of fermentation. The result revealed that starter addition increased ethanol concentration on the fermentation process, increased acetate acid, and citric acid concentratio, meanwhile oxalic acid decreased on cocoa beans during 5 days of fermentation. Depulping caused a slight decrease in acetic acid concentration at the end of fermentation with value of 0,47%, meanwhile the sample of cocoa beans without depulping treatment had acetic acid concentration of 0,49%. Starter culture addition and depulping treatment resulted the best characteristic of cocoa beans which visualized by the largest amounts of cut test score (379 of 400), physical quality (Grade A) and completed SNI No. 2323-2008 requirements with total beans/100 g ratio of 88 beans/100g; pH values of 4,93; acetic acid concentrations of 0,47%, content of fat 15,12%, moisture 4,47%, crudefiber 3,66% and total ash 4,82% after 5 days fermentation.</p>

Study of pH Effect on the Anaerobic-Aerobic Fermentation of Siwalan (Borassus flabellifer L.) Sap to Produce Acetic Acid

2019 ◽  
Vol 964 ◽  
pp. 209-214
Author(s):  
Elly Agustiani ◽  
Atiqa Rahmawati ◽  
Fibrillian Zata Lini ◽  
Dimas Luthfi Ramadhani
Keyword(s):  
Acetic Acid ◽  
Acid Concentration ◽  
Ethanol Fermentation ◽  
Anaerobic Fermentation ◽  
Sugar Content ◽  
Alcoholic Beverages ◽  
Acetic Acid Concentration ◽  
Acetobacter Aceti ◽  
Aerobic Fermentation ◽  
Acid Product

Siwalan (Borassus flabellifer L.) is a palm family that is widely planted in the Tuban area of ​​East Java. Siwalan sap has a relatively high sugar content of about 10-15 g / 100 ml. The sap is obtained by tapping the inflorescences. In general, siwalan sap is used for fresh drinks or alcoholic beverages with maximum storage in 3 days. Based on the sugar content in the sap of siwalan, acetic acid products can be made through fermentation of glucose to ethanol, then the ethanol is fermented into acetic acid. Acetic acid is widely used as a preservative of food and health drinks. The purpose of this research is to study the effect of ethanol fermentation aerobic pH on acetic acid product. Anaerobic fermentation uses saccharomyces cereviceae to produce ethanol, and aerobic fermentation uses acetobacter aceti for acetic acid production. In aerobic ethanol fermentation using pH 3; 3.5; 4 and 5. The concentration of ethanol was analyzed using GC ULTRA Scientific Gas Chromatography, DSQ II detector, and MS 220 column. Acetic acid produced from the aerobic fermentation process was analyzed using an alkalimetric method. Anaerobic fermentation uses Saccharomyces cereviceae with 1-day log phase, while aerobic fermentation uses acetobacter aceti with a 5 day log phase. Aerobic fermentation to produce acetic acid was observed in 5 days to obtained maximum acetic acid concentration, the highest acetic acid concetration is about 2.595 g/l and yield of acetic acid is obtained 0.519% (b/v) at pH 5. Low acetic acid concentration due to low intitial sugar content in siwalan sap.

scholarly journals Isolation and identification of microbial species found in cocoa fermentation as microbial starter culture candidates for cocoa bean fermentation in Colombia.

2020 ◽  
Vol 36 (3) ◽  
pp. 236-248
Author(s):  
Maria Denis Lozano Tovar ◽  
Geraldine Tibasosa ◽  
Carlos Mario González ◽  
Karen Ballestas Alvarez ◽  
Martha Del Pilar Lopez Hernandez ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Starter Culture ◽  
Cocoa Bean ◽  
Rrna Gene ◽  
Cocoa Beans ◽  
Isolation And Identification ◽  
Pichia Kudriavzevii ◽  
Cocoa Bean Fermentation ◽  
Cocoa Fermentation

Microbial activity involved in the cocoa beans fermentation process is essential to maintain and improve the organoleptic and nutritional qualities of chocolate; therefore, the aim of this investigation was to search and select microbial isolates with the potential to improve the quality of cocoa beans. Fermentation experimentswere conducted on farms located in Maceo (Antioquia), San Vicente de Chucurí (Santander), and Rivera and Algeciras (Huila), Colombia. Yeast, lactic acid bacteria (LAB), acetic acid bacteria (AAB), and mesophilic aerobic microorganisms were obtained from different fermentation batches. The growth of these microorganismswas tested in six treatments as follows: 50% cocoa pulp agar (CPA), high concentrations of glucose (10%), ethanol (5%), and acetic acid (7%), an acidic pH of 3.0, and a high temperature of 50oC for 24 h. The isolates with the highest growth were identified by 18S and 16S rRNA gene analysis, revealing a high diversity ofspecies associated with cocoa fermentation, including eight species of yeasts (Debaryomyces hansenii, Meyerozyma guillermondii, Wickerhanomyces anomalus, Pichia guillermondii, Pichia kudriavzevii, Trichosporon asahii, Candida parapsilosis, and Pichia manshurica), six species of LAB (Pediococcus acidilactici, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus farraginis, Lactobacillus rhamnosus, and Leuconostoc mesenteroides), four species of AAB (Gluconobacter japonicus, Acetobacter tropicalis, Acetobacter pasteurianus, and Acetobacter malorum/tropicalis), and three species of Bacillus spp. (Bacillusaryabhattai /megaterium, Bacillus subtilis, and Bacillus coagulans). In general, microbial populations increased in cocoa batches after 12 h of fermentation and decreased after 84-96 h. All the yeast isolates grew in 10% glucose and CPA, 85.7% in 5% ethanol, and 95% at a pH of 3.0. All the yeast isolates were affectedby 7% acetic acid and incubation at 50oC for 24 h. Eighty-five percent of the LAB grew in 10% glucose, 100% in 5% ethanol, 42.8% in CPA, 64% at a pH of 3.0, and 35.7% grew after being exposed to 50oC for 24 h; all were affected by 7% acetic acid. As for the AAB, 100% grew in 10% glucose, 71% in 7% ethanol, 100% grew in CPA, in 7% acetic acid, and at a pH of 3.0, while 100% were affected by incubation at 50oC. Three yeast isolates, W. anomalus, D. hansenii and M. guillermondii, three LAB isolates, P. acidilactici, L. brevis, and L. plantarum, and three AAB isolates, A. tropicalis, A. pasteurianus and G. japonicus, were selected as promising strains to be used in a microbial starter culture for cocoa bean fermentation to improve the organoleptic quality of cocoa.

scholarly journals Sistem Pendukung Keputusan Menentukan Kualitas Pada Biji Kakao Dengan Menggunakan Metode Weighted Product (Wp) (Studi kasus : CV. Bulok Kakao Sentosa Kecamatan Bulok)

Respati ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Elisabet Yunaeti Anggraeni ◽  
Sri Hartati ◽  
Icha Mufadila
Keyword(s):  
Decision Support ◽  
Decision Support System ◽  
Support System ◽  
Theobroma Cacao ◽  
Cocoa Beans ◽  
Dry Land ◽  
Cocoa Production ◽  
Cocoa Farmers ◽  
Theobroma Cacao L

INTISARITanaman kakao (Theobroma cacao, L.) atau yang biasa disebut coklat merupakan tanaman perkebunan yang tumbuh di lahan kering. Tanaman kakao merupakan salah satu komoditas unggulan nasional yang berperan penting bagi pertumbuhan perekonomian Indonesia. Namun, dalam  budidayanya petani kakao seringkali menghadapi masalah yang dapat menyebabkan penurunan kualitas hasil produksi kakao. Karena permasalahan inilah yang membuat peneliti ingin membuat suatu sistem pendukung keputusan yang dapat membantu petani dan suplayer terutama CV. Bulok Kakao Sentosa untuk menentukan kualitas biji kakao. Sistem yang dirancang menggunakan metode Weighted Product (WP) merupakan metode penyelesaian dengan menggunakan perkalian untuk menghubungkan nilai atribut, dimana nilai harus dipangkatkan terlebih dahulu dengan bobot atribut yang bersangkutan. Dengan adanya sistem  penunjang keputusan ini mampu membantu masyarakat untuk menentukan  kualitas biji kakao dan di harapkan sistem pendukung keputusan menggunakan metode Weighted Product dalam proses menentukan kualitas pada biji kakao tersebut dapat menyelesaikan masalah pada CV. Bulok Kakao Sentosa, petani dan pengepul kakao dalam menentukan kualitas biji kakao yang baik Kata Kunci : Sistem Penunjang Keputusan, Weighted Product (WP), Biji Kakao ABSTRACTCacao (Theobroma cacao, L.) or commonly called cocoa is an estate crop that grows on dry land. Cacao is one of the leading national commodities that plays an important role in Indonesia's economic growth. However, in the cultivation of cocoa farmers often face problems that can cause a decrease in the quality of cocoa production. Because of this problem that makes researchers want to create a decision support system that can help farmers and suppliers, especially CV. Bulok Kakao Sentosa to determine the quality of cocoa beans. The system is designed using the Weighted Product (WP) method is a method of settlement using multiplication to connect the attribute value, where the value must be raised first with the weight of the attribute in question. With this decision support system able to help the community to determine the quality of good cocoa beans and expected with the decision support system using the Weighted Product method in the process of determining the quality of the cocoa beans can solve the problem in the CV. Bulok Kakao Sentosa, cocoa farmers and collectors in determining the quality of good cocoa beans. Keywords : Decision Support System, Weighted Product (WP), Cacao Beans

scholarly journals Characterization of Polyphenols from Various Cocoa (Theobroma cacao L.) Clones During Fermentation

2018 ◽  
Vol 34 (2) ◽  
pp. 104-112
Author(s):  
Muhammad Isa Dwijatmoko ◽  
Budi Nurtama ◽  
Nancy Dewi Yuliana ◽  
Misnawi Misnawi
Keyword(s):  
Theobroma Cacao ◽  
Antioxidant Property ◽  
Cocoa Bean ◽  
Flavor Quality ◽  
Cocoa Beans ◽  
Total Polyphenols ◽  
One Step ◽  
Theobroma Cacao L

Cocoa bean is a rich source of polyphenols, which are the largest group secondary metabolite with natural antioxidant property. Polyphenols from cocoabeans was reported to possess health benefits. Fermentation, one step in cocoa processing is needed to improve the quality of cocoa in which the concentration of cocoa bean polyphenols might decrease significantly through oxidation and exudation. Cocoa polyphenols content among different cocoa clones mightalso vary. The aims of this study were to determine total polyphenols, total flavanoid, epicatechin, and catechin content in several cocoa clones, those wereSulawesi 1, Sulawesi 2, ICCRI 03, and KW 617. Until now, characterization of polyphenols from those clones has not been reported. The effect of five daysfermentation to those parameters was also studied. The results of the study showed that fermentation and type of clones significantly affected total of polyphenols, total of flavanoids, epicatechin, and catechin content of the cocoa, there is also an interaction between fermentation and type of clones. Unfermented of Sulawesi 1 had the highest total polyphenols of 96.94±5.83 mg/g, total flavanoids of 90.92±1.89 mg/g, epicatechin of 52.50±0.46 mg/g, and catechin of 1.99±0.02 mg/g content compared to other clones. Among five days fermented cocoa beans, Sulawesi 2 showed the highest total polyphenols and total flavanoids content, while ICCRI 03 had the highest epicatechin and catechin content than other clones. Thus, in can be concluded that although fermentation is required to improve the flavor quality of cocoa, it significantly reduced the content of bioactive compounds. This effect varied amongdifferent cocoa clones.

scholarly journals Kajian Beberapa Cara Fermentasi yang Dilakukan oleh Petani terhadap Mutu Biji Kakao (Theobroma cacao L.)

2019 ◽  
Vol 8 (2) ◽  
pp. 189
Author(s):  
NI LUH MADE PRADNYAWATHI ◽  
I KETUT ARSA WIJAYA ◽  
I NYOMAN SUTEDJA ◽  
ANAK AGUNG MADE ASTININGSIH
Keyword(s):  
Theobroma Cacao ◽  
Field Survey ◽  
Literature Study ◽  
Cocoa Beans ◽  
Interview Method ◽  
Fermentation Method ◽  
Banana Leaves ◽  
Theobroma Cacao L ◽  
Local Farmers

Study of Assorted Fermentation Methods Conducted by Local Farmers on the Quality of VCocoa Beans (Theobroma Cacao L.). Various fermentation methods have been done by local farmers, however there has been no report on the quality of cocoa beans from those methods. This study aims were to identify various ways of cocoa beans fermentation conducted by local farmers; to compare between quality of fermented beans produced by local farmers and suggested quality of fermented beans; and o obtain a simple fermentation method which resulted in good quality beans and suitable practice for local farmers. The research wasconducted in Tabanan Regency, Province of Bali through field survey with observation and interview method, and supported with literature study. According to field observation and analysis of fermented cocoa beans taken from the local farmers, it can be concluded that most of local farmers did not fermented their kakao seeds. Thus, the fermentation done by utilizing a plastic sack, a bamboo basket or a wooden box lined and covered with banana leaves. The research also found that implementing similar fermentation methods to the seed did not always resulting similar quality of cocoa beans. Finally, the simplest method for local farmers to ferment the cocoa seed was the fermentation by utilizing basket lined and covered with banana leaves with at least 90kg wet beans.

scholarly journals ISOLASI DAN KARAKTERISASI GELATIN DARI TULANG AYAM DENGAN METODE ASAM

ALCHEMY ◽  
2013 ◽  
Author(s):  
Akyunul Jannah ◽  
Anik Maunatin ◽  
Arin Windayanti ◽  
Yuana Findianti ◽  
Zulfiatul Mufidah
Keyword(s):  
Acetic Acid ◽  
Acid Concentration ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Raw Materials ◽  
Extraction Temperature ◽  
Acetic Acid Concentration ◽  
Chicken Bone

<p>The purpose of this study was to determine the quality of gelatin by using raw materials bone chicken (Gallus gallus domesticus) and the Broiler chickens for differences in the concentration of acetic acid immersion process ( curing ).The method used in this study is the preparation and broiler chicken bones , isolation of gelatin with various concentration of acetic acid in the curing process , the process of hydrolysis , extraction temperature rise and gelatin with gelatin obtained include the characterization of protein , water , ash and metal.</p>The results showed the highest yield of gelatin produced from Broiler chicken bone types with acetic acid concentration of 1 % , ie 3.25%. Gelatin highest protein content of 86.27 % of the types of Broiler chicken bone with acetic acid concentration of 1.5 %. Lowest ash content of 15.7 % gelatin from bone types Broiler chicken with 1 % acetic acid concentration , and from analysis using AAS contained Cu at 0.6 % (of the type of chicken bones, acetic acid concentration of 0.5 %) and Cu metal content of 1.1 % (of the type of Broiler chicken bones, acetic acid concentration of 1 %. highest moisture content of 0.17 % gelatin from bone types Kampung chicken with 1 % acetic acid concentration

scholarly journals Fermented Cocoa Beans (Theobroma cacao L) in Southeast Aceh District

2021 ◽  
Vol 2 (1) ◽  
pp. 13
Author(s):  
Syariani Tambunan ◽  
Nico Syahputra Sebayang ◽  
Desi Sri Pasca Sari
Keyword(s):  
Theobroma Cacao ◽  
International Markets ◽  
Cocoa Beans ◽  
Agricultural Industry ◽  
Exchange Value ◽  
Cocoa Products ◽  
Cocoa Production ◽  
Cocoa Farmers ◽  
Theobroma Cacao L

One of the productions of the agricultural industry that has a fairly high foreign exchange value for our country is cocoa, but our cocoa production is still of low quality. To get good quality cocoa, good processing must be done. Fermentation of cocoa beans is one way to improve the quality of cocoa so that it can be sold in national and international markets. The purpose of this activity is to help cocoa farmers, especially in Southeast Aceh district, in improving the quality of cocoa production, so that the cocoa products of Southeast Aceh farmers can compete in trade both locally and abroad. The implementation of this activity is carried out through training to improve the quality of cocoa. There are several activities, namely: Sorting Cocoa, peeling cocoa pods, fermenting cocoa pods for 5 days and the last step is drying until the moisture content of cocoa pods reaches 7%. The result of this activity is to increase the knowledge of cocoa farmers in Southeast Aceh Regency in order to improve the quality of cocoa beans, so that cocoa sales can be accepted and compete in the market.

scholarly journals Pengaruh Kadar Air dan Persamaan Model Bet untuk Prediksi Masa Simpan Kakao (Theobroma cacao L.)

2017 ◽  
Vol 9 (1) ◽  
pp. 17-22
Author(s):  
Rita Hayati
Keyword(s):  
Shelf Life ◽  
Water Content ◽  
Theobroma Cacao ◽  
Cocoa Beans ◽  
Drying Temperature ◽  
Drying Up ◽  
Storage Area ◽  
Theobroma Cacao L ◽  
Bet Equation

Effect of moisture content and BET equation to the shelf life of cocoa (Theobroma cacao L.) has been conducted. Increasing the quality of cocoa beans is with proper post-harvest handling, including how the harvest, the level of ripeness, curing, fermenting, drying up storage. The purpose of this study was to determine the water content and the appropriate levels of critical in determining the shelf life of cocoa. The benefits of this research are as information for farmers in determining the shelf life of cocoa in order to design a storage area corresponding to the respiratory system owned by cocoa. The results showed that the effect of fermentation facilitator (Staphilococcus cerevisiae) and drying temperature on water content showed that the fermentation facilitator (Staphilococcus cerevisiae) highly significant effect on water content. The drying temperature is also highly significant effect on water content. BET equation obtained was Y = 2,912x + 1.238 (R2 = 0.965), Y = 2,897x + 1.353 (R2 = 0.968) and Y = 2,806x + 1.89 (R2 = 0.954).

scholarly journals PERUBAHAN pH , KEASAMAN DAN INDEKS FERMENTASI BIJI KAKAO SELAMA FERMENTASI HASIL BIJI KAKAO (THEOBROMA CACAO)

2020 ◽  
Author(s):  
mulono apriyanto bin sugeng rijanto
Keyword(s):  
Acetic Acid ◽  
Theobroma Cacao ◽  
Fermentation Process ◽  
Acetic Acid Bacteria ◽  
The Sun ◽  
Dry Beans ◽  
Cocoa Beans ◽  
Fermentation Method

Most cocoa beans are produced by farmers Indonesia is dry cocoa drying whosequality can be improved by the fermentation method, but it takes more optimizationfor fermentation can run well. This study aimed to determine changes in the chemicalproperties of fermented dry cocoa beans drying. Dry beans drying is obtained bydrying the wet cocoa beans (fresh) in a cabinet dryer with a previously conditionedat a temperature such as drying in the sun, and each determined the reduction ofsugar levels. Experiments fermented dry cocoa beans are fermented in thefermentation container with the amount of 100 grams of seed per container. Beforethe first fermented dry cocoa beans drying on rehydration to obtain a moisturecontent approaching fresh beans, dry beans drying then incubated for six days andwithout inverted during fermentation. Each treatment was repeated three times andobserved every 24 hours to 120 hours. Reduction sugar, acid levels tertitrasi, thepopulation of yeasts and acetic acid bacteria in fermented pulp was observed duringthe fermentation process. To determine the quality of dry beans drying measurementof pH and the fermentation index during fermentation.

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