INCREASING PRODUCTION WEIGHT OF MODIFIED-BIOSILICA FROM RICE HUSK AND ITS APPLICATIONS AS REINFORCING FILLER IN RUBBER FINISHED GOODS

Biosilica from rice husks has a potential that can be used as a reinforcing filler in the production of rubber finished goods displace mine silica. But it’s difficult to disperse homogenetically into a rubber finished goods, so it needs to modify the surface using a coupling agent sign. The capacity for the production of modified-biosilica would need to be at a great level to meet the needs of the rubber industry. The purpose of the research is to identify the characteristics of the modified-biosilica by silane TESPT (bis-(3-triethoxysilylpropyl) tetrasulfane) that resulted from increasing the weight of biosilica in the production process and the application as reinforcing filler on the rubber. Increasing of weight in the production process was done gradually on a scale of 0.5; 1; 2; and 5 kg of biosilica per process using a mixer-propeller. The technique on surface modifications was using a one-step modification (OSM) and two-step modification (TSM). Surface modification treatment using TESPT has increased the uniformity of the particle size distribution of the biosilica. Unmodified-biosilica has an average particle size of 717.1 nm (PDI 0.600), while modified-biosilica has an average particle size of 574.6 nm (PDI 0.585). Applications for reinforcing filler in the soles rubber industry are performed in PT Triangkasa Lestari Utama. Research indicates that increasing the weight of biosilica on the surface modifications did not significantly affect the density, lightness, crystallinity, and purity. The applications as reinforcing fillers have increased the quality of rubber finished goods compared with unmodified-biosilica. The best rubber finished goods quality approaching shoes-sol standards is a product that used modified-biosilica by OSM technique. This rubber finished goods has a tensile strength of 5.80 MPa, elongation at break of 425%, tear strength of 23.25% and abrasion resistance of 251.5 mm3 .

ANTIOXIDANT ACTIVITY, DEXTROSE EQUIVALENT, TOTAL DISSOLVED SOLIDS, AND VISCOSITY OF MALTED RED RICE MILK AT DIFFERENT ENZYME CONCENTRATIONS

Rice milk is a plant-based milk-like product that can be an alternative for people who have an allergy to bovine milk and lactose intolerance. Malting and enzymatic hydrolysis can bring out the natural sweetness of the rice milk product and thus minimize the use of sweeteners. The current research aimed to determine the total value of dissolved solids, viscosity, dextrose equivalent, and antioxidant activity in malted red rice milk with the addition of glucoamylase at different concentrations. The red rice was malted for 48 hours before drying and milling. The resulted malt powder was then diluted and added with glucoamylase enzyme at the concentration of 0%, 1%, 2%, and 3%. Enzymatic hydrolysis occurred at 60°C for 3 hours. Completely Randomized Design was used for the parameters of total soluble solids, viscosity, and dextrose equivalent while the antioxidant activity parameter was analyzed descriptively. The result showed that the addition of glucoamylase significantly (p<0.05) increased the total soluble solids, viscosity, and dextrose equivalent. The highest values were 9.960 °Brix, 1620 cP, and 1.872 for total dissolved solids, viscosity, and dextrose equivalent, respectively. Increasing antioxidant activity was also observed from 6.094% at 0% glucoamylase to 10.762% at 3% glucoamylase addition.

ANTIBACTERIAL ACTIVITY OF TEA, BREADFRUIT, AND PIPER BETLE LEAVES EXTRACTS AGAINST Propionibacterium acnes AND ITS APPLICATION AS ANTI-ACNE MASK

Tea, breadfruit, and piper betle leaves contain bioactive components that have a function as an antibacterial. The purpose of this study was to determine the type of leaf extract that has the highest inhibition power against Proponibacterium acnes and determine the concentration of the selected extract for mask applications that have good physical characteristics and acceptable to panelists. Extraction of tea, breadfruit, and piper betel leaves were carried out with 96% ethanol solvent using maceration method for 24 hours and concentrated with a rotary evaporator. Thick extracts contribute to antibacterial activity by disk diffusion method and total phenolic content. The selected extract was formulated into a clay mask with concentration of 0.5%, 1%, and 1.5%. The result showed that breadfruit leaf extract had the highest inhibition power against Propionibacterium acnes which was 10.844 ± 0.327 mm (strong inhibition category) so that extract can be applied to clay mask. Breadfruit leaf extract concentration of 1.5% was the chosen formulation because it meets the standards, approved by the panelists and the most effective antibacterial for acne problems.

EFFECT OF COATING ON THE PHYSICAL AND MORPHOLOGICAL QUALITY OF FRESH HEN EGG DURING STORAGE

<p>Hen egg is the most consumed animal food due to its high nutritional content, affordable and easy to obtain. However, eggs have the disadvantage for their short shelf life of about 7 days at room temperature. An effort to extend the shelf life of eggs including storing at low temperatures and/or coating. The research objective was to determine changes in the physical quality and morphology of chicken eggs coated with two material coating stored at room (25–29 °C) and AC/low (18–22 °C) temperature. The treatments tested were two kinds of coatings, namely gelatin and lime solution, and two storage temperature conditions, namely room and low temperature for 30 days. The study was conducted with three replications, each replication was observed on 5 eggs. The parameters observed were weight loss, Egg white index, Egg yolk index, air cavity, Haugh Unit and surface morphology. The results showed that at the end of storage for 30 days the eggs quality still met the SNI standards I to III. AC storage temperature was better than room temperature. The egg treated with coating produced better quality than without coating. The longer the storage, the lower the weight loss, HU, IPT and IKT. The best treatment was gelatin coating stored at AC temperature.</p>

THE EFFECT OF CHICKEN FEET GELATIN TO CHARACTERISTIC OF JELLY CANDY

Jelly candy is a soft textured candy processed with hydrocolloid components such as gum, pectin, agar, starch, carrageenan, gelatin. These are used to modify the chewy texture. This study aimed was to determine the effect of the combination of chicken feet gelatin and agar on the quality of jelly candy. Processing of chicken feet gelatin jelly candy based on Caeccar et.al (2018) method by modification. The study used a completely randomized design (CRD) with one factor, five levels and three replications. The factors studied were the ratio of chicken feet gelatin and agar 80:20; 60:40; 40:60; 20:80; 0:100. Product quality characteristics observed in this study were water content, ash content, reducing sugar content, sucrose content, and organoleptic quality. The results showed that the combination of chicken feet gelatine and agar were significantly affected water, ash, reducing sucrose content. Jelly candy with a treatment combination of 80% chicken feet gelatine and 20% agar was the best treatment. The results of the analysis of the candy were water content of 11.02± 0.04%, ash content of 0.89± 0.00%, reducing sugar content of 10.83± 0.01%, sucrose content of 50.68± 0.28%. The organoleptic test results showed that chicken feet gelatine improved the texture and taste of jelly candy.

INHIBITING ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME-1 BY BEAN PROTEIN HYDROLYSATE GENUS PHASEOLUS

Kidney bean (Phaseolus.vulgaris L. Chitra) and lima bean (Phaseolus lunatus L. Sweet) hydrolysates were obtained by alcalase and flavourzyme hydrolysis of the bean seed protein. Peptide in the bean hydrolysates, with hydrophobic amino acids had been studied for their inhibitory ACE-1 activity preventing transformation into ACE-2 that prevention hypertension. This study aimed to measure inhibitory ACE-1 activity of protein hydrolyzates from the bean Phaseolus genus spp. grown in Jember, and its solubility. The bean protein (19.8-20,2%) was extracted using isoelectric precipitation at pH 4-4,6. The extract were hydrolyzed at pH 8-9 for alcalase and pH 7 for flavourzyme, followed with inactivation at 80-85 o C. ACE-1 inhibitory activity was measured based on the amount of hippuric acid (HA) formed by the hydrolysis of Hippuryl-His-Leu (HHL) in spectrophotometry detection method (228 nm). The ultra chromatography evaluation showed that the protein hydrolysates of kidney bean contained higher hydrophobic amino acids (455.5 mg/g protein) compare to those of lima bean (350 mg/g protein). Protein hydrolysates of both beans from alcalase hydrolysis have higher ACE-1 inhibitory rather than those from flavourzyme. Protein hydrolysate from Phaseolus spp bean protein hydrolysis by alcalase, contain small molecular weight peptides (3.9-22.6 kDa) high ACE-1 inhibition ability (83 -88%), and therefore suggested as antihypertensive nutraceuticals. Highest solubility of protein hydrolysate resulted from alcalase hydrolysis of both beans were observed at pH 8-9, while those resulted from flavorzyme hydrolysis were at pH 7.

CHARACTERIZATION OF SORGUM MUFFIN (Sorghum bicolor) OF KD 4 VARIETY WITH SOAKING GRAIN AND SORGUM FLOUR CONCENTRATION TREATMENT

<p class="Abstrak"><span lang="EN-GB">By processing sorghum grains into flour, the use of sorghum grains to support food diversification can be made more varied. The research objective was to determine the physical and chemical properties of sorghum muffins and changes of physical properties of sorghum muffins during storage. The research uses a completely randomized design with two factor, grain soaking duration (hours) and ratio of concentration of whole sorghum flours with gluten flour (%) and consists of nine treatments combination. The result shows that treatment has a significant effect on the power expansion, degree of brightness, fat, and preference for organoleptic properties. The best treatment was 24-hours of soaking, 25% whole sorghum flour, 75% gluten flour that produces expanded power at 138,83%, degree of brightness (L) at 67.03, redness (a) at +4.33, yellowness (b) at +27.37, water content of 12.59%, protein content of 6.95%, fat content of 2.46%, score of color at 3.20, texture score at 3.13, aroma score at 3.16, taste score at 3.24, and for overall acceptance at 3.53 (like). Changes of physical properties of sorghum muffins during storage for 3 days can be explained with the linear equation. For all parameter (weight loss, volume loss, brightness loss), the value of constant a in the equation of sorghum muffin was lower than control muffin that indicated quality loss of sorghum muffins is higher than control muffin. Increasing the concentration of sorghum flour resulted a decrease in the quality of the muffins. The soaking treatment of the seeds in making sorghum flour can maintain the quality of the muffin.</span></p>

CHARACTERISTIC OF PHYSICOCHEMICAL AND SENSORY CASSAVA STICK WITH THE SUBSTITUTION OF FERMENTED CASSAVA BAGASSE FLOUR

<div class="WordSection1"><p class="Abstrak"><span lang="EN-GB">Part of the cassava that can be used as a food product is usually starch and and the waste is cassava bagasse. Fermented cassava bagasse flour is a modified semi-solid fermentation product using yeast <em>Saccharomyces</em> <em>cerevisiae </em>which has almost the same quality as modified cassava flour (mocaf), but fermented cassava bagasse flour has advantages compared to mocaf flour, namely high protein content and cheaper production costs. Fermented cassava bagasse flour has various functions which can be used as raw material for the manufacture of various processed products, one of which is cassava stick. The research objective was to see the formulation of fermented cassava bagasse flour to the sensory characteristics of the resulting cassava stick. The study was conducted using a completely randomized design (CRD) including 5 treatment methods, namely cassava starch substituted by fermented cassava bagasse flour 5% (B1), 10% (B2), 15% (B3), 20% (B4) and 25% (B5), each treatment was repeated 3 times. Based on the sensory results, it was obtained that 15% (B3) fermented cassava bagasse flour substitution treatment was the best choice of cassava stick product by panelists. The results of the analysis of cassava stick products were hardness test 3,77 µ (kg / cm²) ; swelling ratio 18,33% ; moisture content 2,20% ; ash content 0,14% ; protein content 10,00% ; fat content 21,00% ; fiber content 0,17%; carbohydrate content 66,49% and total dietary fiber 21,24%.</span></p></div>

OPTIMIZATION OF RESISTANT STARCH FROM BANANA FLOUR CV. MAS KIRANA OFF GRADE TO PRODUCE YOGURT PREBIOTIC

<p class="Abstrak"><span lang="EN-GB">The banana of CV. Mas Kirana off-grade can be processed into banana flour with high resistant starch content which functions as a prebiotic. This study aims to optimize the content of resistant starch (RS) type 3 in the produce of banana flour. Modified banana flour cv. Mas Kirana with highest RS content will then be used to substitute skim milk in making prebiotic banana yogurt and are analyzed for its sensory properties. Data were processed using design expert 11.0 with three factors, namely X1 (Lactic acid bacterial concentration (LAB), X2 (fermentation time), X3 (retrogradation time). The optimization result was 10⁹ CFU/mL of lactic acid bacteria concentration, 24 hours of fermentation time, and 37,60 hours of retrogradation time. The resulting banana flour cv Mas Kirana has 83,95% of resistant starch, 29,24% of yield, 3,60 of logarithmic lactic acid bacteria, and 4,49 of pH value. Its application to the production of banana yoghurt prebiotic replacing 80% skim milk, was acceptable to panelists in terms of color, aroma, viscosity, taste, and overall acceptance.</span></p>

EXTRACTION, CHARACTERIZATION AND APPLICATION OF CHITOSAN DERIVED FROM MAGGOT (BLACK SOLDIER FLY) WASTE AS EDIBLE COATING FOR RED GRAPES (Vitis vinifera)

<p class="Abstrak"><span class="tlid-translation"><span lang="EN-GB">Black Soldier Fly (BSF) farming showed an increasing trend in the past 5 years. The production process generates a leftover, that is the exuviae which are still untapped. BSF exuviae contain chitin and chitosan which can be utilized in various fields, one of which is fruit preservatives. Grapes (<em>Vitis vinifera</em>) is known to have many benefits and has a content of vitamins A, B, C, and E but it has a short shelf life. The purpose of this study was to characterize chitosan from BSF exuviae and choose the best edible coating method to be applied to grapes to prolong the grape’s storage time. Characterization includes physical condition, percent solubility, degree of deacetylation, and yield of the chitosan. Then, edible coating tests were carried out with 2% acetic acid solvent followed by two different methods, namely dyeing method with 5, 10 and, 15 minutes immersion time and spray method with a chitosan solution concentration of 0,5%, 1%, 1,5%, 2 %, and 2,5%. The results showed that chitosan extracted from BSF exuviae produced a physical appearance in the form of white brown powder with 55% solubility and a degree of deacetylation of 91,88%. The chitin yield is 16,2%, followed by the chitosan yield of 4,8%. The best edible coating method which can maintain the physical appearance of grapes and inhibiting fruit decayed was obtained by dipping method at a concentration of 2,5% for 10 minutes. Based on the overall observations, however, the results obtained from the dip and spray method were not significantly different.</span></span></p>