scholarly journals Optimum Combination of Reversed-Phase Column Type and Mobile-Phase Composition for Gradient Elution Ion Pair Chromatography of Amino Acids.

1997 ◽  
Vol 13 (6) ◽  
pp. 963-967 ◽  
Author(s):  
Yukio YOKOYAMA ◽  
Tomoo AMAKI ◽  
Sohei HORIKOSHI ◽  
Hisakuni SATO
Keyword(s):  
Mobile Phase ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Ion Pair ◽  
Optimum Combination ◽  
Mobile Phase Composition ◽  
Ion Pair Chromatography ◽  
Column Type ◽  
Phase Column ◽  
Reversed Phase Column

Improved separation of C12-C22 fatty acid phenacyl esters by reversed phase column liquid chromatography

1986 ◽  
Vol 51 (12) ◽  
pp. 2722-2726 ◽  
Author(s):  
Tomáš Haniš ◽  
Miroslav Smrž ◽  
Pavel Klír ◽  
Karel Macek ◽  
Zdeněk Deyl
Keyword(s):  
Volume Concentration ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Isocratic Elution ◽  
Water Gradient ◽  
Phenacyl Esters ◽  
Acetonitrile Concentration ◽  
Phase Column ◽  
Reversed Phase Column ◽  
Critical Pairs

Phenacyl esters of C12-C22 fatty acids were separated on Separon SGX C18 column, using a gradient elution with methanol-acetonitrile-water. The proposed gradient showed better resolution of the critical pairs C18:3-C14:0, C16:1-C20:4, and C16:0-C18:1 than the gradient elution with methanol-water or acetonitrile-water, or than the isocratic elution with methanol-acetonitrile-water. The optimum volume concentration (83%) of the sum of both methanol and acetonitrile was maintained constant for 35 min; in this period the acetonitrile concentration decreased linearly from the initial 42-60% to 0% while the methanol concentration increased from the initial 41-23% to 83% at the same rate. After 35 min the elution was completed with a methanol-water gradient. The whole analysis can be performed within 63 min at a flow rate 1 ml/min.

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

scholarly journals HPLC FINGERPRINT OF ORGANIC ACIDS IN FRUIT JUICES

1970 ◽  
Vol 62 ◽  
Author(s):  
Loredana Leopold ◽  
Diehl Horst ◽  
Carmen Socaciu
Keyword(s):  
Organic Acids ◽  
Stationary Phase ◽  
Organic Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Fruit Juices ◽  
Hplc Fingerprint ◽  
Organic Acid Profile ◽  
Phase Column ◽  
Reversed Phase Column

Organic acids give fruit products their characteristic tartness and vary in combination and in concentrations among different juices. The organic acid profile can be used to identify a juice or verify its purity. Typically, organic acids in fruit juices are identified and quantified by using methods such as HPLC. In this procedure, reversed phase column is used to separate and identificate six organic acids. Because several of the analytes are extremely difficult to resolve, a aqueous mobile phase is needed to enhance interaction between the acids and the C18 stationary phase.

Reversed-phase ion-pair chromatography with UV-absorbing ions in the mobile phase

1981 ◽  
Vol 218 ◽  
pp. 31-43 ◽  
Author(s):  
M. Denkert ◽  
L. Hackzell ◽  
G. Schill ◽  
E. Sjögren
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Ion Pair ◽  
Ion Pair Chromatography

scholarly journals Observations on Ion Interaction Chromatographic System: Reversed Phase Column—H3BO3/TBAOH Mobile Phase and the Effect of Temperature

2020 ◽  
Vol 83 (12) ◽  
pp. 1553-1560
Author(s):  
Rajmund S. Dybczyński ◽  
Krzysztof Kulisa
Keyword(s):  
Ion Exchange ◽  
Boric Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Effect Of Temperature ◽  
Chromatographic System ◽  
Retention Loss ◽  
Hydrophobic Adsorption ◽  
Phase Column ◽  
Reversed Phase Column

Abstract New ion interaction chromatographic (IIC) system with RP column and boric acid plus tetra-n-butylammonium hydroxide (TBAOH) mobile phase was investigated. In the system: CPhenylHexyl—10 mM H3BO3/4 mM TBAOH, a large group of anions viz. F−,IO3−, Cl−, ClO2−, BrO3−, NO2−, Br−, NO3−, ClO3−, I−, HPO42−, SO42−, CrO42−, S2O32−, benzoate−, SCN−, ClO4− and phthalate2−, could be isocratically resolved. A study on the effect of temperature revealed that some ions added to ion exchange are also held in the stationary phase by the hydrophobic adsorption. The retention loss for all anions with time was observed. This effect however, was quite slow and good separations could be obtained even after the column stayed a few hundred hours in the mobile phase.

Liquid-chromatographic quantification of plasma phenylalanine, tyrosine, and tryptophan.

1981 ◽  
Vol 27 (1) ◽  
pp. 146-148 ◽  
Author(s):  
L M Neckers ◽  
L E Delisi ◽  
R J Wyatt
Keyword(s):  
Amino Acids ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Plasma Phenylalanine ◽  
Liquid Chromatographic ◽  
Phase Column ◽  
Reversed Phase Column

Abstract Phenylalanine, tyrosine, and tryptophan are isolated and quantified by "high-pressure" liquid chromatography, with fluorescence detection. An isocratic mobile phase and reversed-phase column are used to provide rapid and reproducible measurement of these amino acids in as little as 1 to 2 microL of human plasma.

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