scholarly journals HPLC FINGERPRINT OF ORGANIC ACIDS IN FRUIT JUICES

1970 ◽  
Vol 62 ◽  
Author(s):  
Loredana Leopold ◽  
Diehl Horst ◽  
Carmen Socaciu
Keyword(s):  
Organic Acids ◽  
Stationary Phase ◽  
Organic Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Fruit Juices ◽  
Hplc Fingerprint ◽  
Organic Acid Profile ◽  
Phase Column ◽  
Reversed Phase Column

Organic acids give fruit products their characteristic tartness and vary in combination and in concentrations among different juices. The organic acid profile can be used to identify a juice or verify its purity. Typically, organic acids in fruit juices are identified and quantified by using methods such as HPLC. In this procedure, reversed phase column is used to separate and identificate six organic acids. Because several of the analytes are extremely difficult to resolve, a aqueous mobile phase is needed to enhance interaction between the acids and the C18 stationary phase.

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

scholarly journals Observations on Ion Interaction Chromatographic System: Reversed Phase Column—H3BO3/TBAOH Mobile Phase and the Effect of Temperature

2020 ◽  
Vol 83 (12) ◽  
pp. 1553-1560
Author(s):  
Rajmund S. Dybczyński ◽  
Krzysztof Kulisa
Keyword(s):  
Ion Exchange ◽  
Boric Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Effect Of Temperature ◽  
Chromatographic System ◽  
Retention Loss ◽  
Hydrophobic Adsorption ◽  
Phase Column ◽  
Reversed Phase Column

Abstract New ion interaction chromatographic (IIC) system with RP column and boric acid plus tetra-n-butylammonium hydroxide (TBAOH) mobile phase was investigated. In the system: CPhenylHexyl—10 mM H3BO3/4 mM TBAOH, a large group of anions viz. F−,IO3−, Cl−, ClO2−, BrO3−, NO2−, Br−, NO3−, ClO3−, I−, HPO42−, SO42−, CrO42−, S2O32−, benzoate−, SCN−, ClO4− and phthalate2−, could be isocratically resolved. A study on the effect of temperature revealed that some ions added to ion exchange are also held in the stationary phase by the hydrophobic adsorption. The retention loss for all anions with time was observed. This effect however, was quite slow and good separations could be obtained even after the column stayed a few hundred hours in the mobile phase.

Liquid-chromatographic quantification of plasma phenylalanine, tyrosine, and tryptophan.

1981 ◽  
Vol 27 (1) ◽  
pp. 146-148 ◽  
Author(s):  
L M Neckers ◽  
L E Delisi ◽  
R J Wyatt
Keyword(s):  
Amino Acids ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Plasma Phenylalanine ◽  
Liquid Chromatographic ◽  
Phase Column ◽  
Reversed Phase Column

Abstract Phenylalanine, tyrosine, and tryptophan are isolated and quantified by "high-pressure" liquid chromatography, with fluorescence detection. An isocratic mobile phase and reversed-phase column are used to provide rapid and reproducible measurement of these amino acids in as little as 1 to 2 microL of human plasma.

scholarly journals Determination of Organic Acids in Fruit juices by UPLC

2015 ◽  
Vol 9 (5) ◽  
pp. 41-44 ◽  
Author(s):  
Fereshteh Khosravi ◽  
Nahid Rastakhiz ◽  
Behzad Iranmanesh ◽  
Seyyed Sina Seyyed Jafari Olia
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Sweet Orange ◽  
Electron System ◽  
Reversed Phase ◽  
Lemon Juice ◽  
Fruit Juices ◽  
Grape Fruit ◽  
Array Detection

A reversed phase UPLC method for separation and quantification of organic acids (oxalic, citric, tartaric, malic and ascorbic and lactic acids) in fruit juices was developed. The chromatographic separation was performed with a Surveyor Thermo Electron system at 10°C by using a potassium dihydrogen orthophosphate buffer (pH3.1) as mobile phase, an Hypersil Gold a Q Analytical Column and diode array detection at λ=254 nm for ascorbic acid and λ=214 nm for the other organic acids. Organic acid profiles of seven species of fruits: sweet orange, white and red apple, lemon, lime, white and pink grape fruit were established. Species significantly affect the organic acid distribution of fruit juices. In all juices, the most abundant organic acid was citric acid, ranging from 5.22 to 62.42g/l. Fruit juices are good sources of ascorbic acid (0.137-0.625g/l). The average ascorbic acid was the highest in lemon juice followed by sweet orange juice, sweetie and white grapefruit.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12690

scholarly journals Organic Acid Profile of “Batuan” (Garcinia binucao [Blco] Choisy) Fruit

2017 ◽  
pp. 25-33 ◽  
Author(s):  
Elizabeth Quevedo ◽  
Erlinda Dizon ◽  
Florinia Merca
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Oxalic Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Fumaric Acid ◽  
Mobile Phase ◽  
Fruit Samples ◽  
Organic Acid Profile

“Batuan” fruit (Garcinia binucao [Blco.] Choisy), an indigenous acidulant grown in the Visayas State University, Baybay City, Leyte was analyzed for its organic acid profile at different stages of maturity for the development of potential food and non-food products. The analysis of organic acid content was done using Reverse Phase-High Performance Liquid Chromatography. Organic acids in the dried, powdered “batuan” fruit samples were extracted with the mobile phase (50mM KH2PO4/ H3PO4, pH2.8). The sample extracts and organic acid standards (oxalic acid, tartaric acid, malic acid, citric acid, fumaric acid, lactic acid, acetic acid, and succinic acid) were injected to RP-HPLC under isocratic elution with the mobile phase at a flow rate of 1.0mL min-1 and using UV-vis detection at 210nm. “Batuan” fruit samples contain oxalic acid, tartaric acid, malic acid, citric acid, fumaric acid, succinic acid, acetic acid, lactic acid, and a few unidentified organic acids. Among the organic acids present, citric acid accumulated the highest in the ripe “batuan” fruit; fumaric acid, the least. Results of this study show that “batuan” fruit could be a good natural source of acidulant for food and non-food applications.

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