scholarly journals Comprehensive investigation of the prevalence and risk factors of viral hepatitis B and C in PERSIAN Guilan Cohort Study

2019 ◽  
Author(s):  
Farahnaz Joukar ◽  
Mohammadreza Naghipour ◽  
Soheil Hassanipour ◽  
Sara Yeganeh ◽  
Soheil Soltanipour ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Urine Analysis ◽  
Health Impact ◽  
Pcr Assay ◽  
Serum Samples ◽  
Hcv Prevalence ◽  
Positive Serum ◽  
Viral Hepatitis B ◽  
Hcv Genotyping

Abstract Background Hepatitis B (HBV) and C (HCV) viruses are two serious infectious diseases with high global health impact. The aim of this study was to evaluate the prevalence of HBV and HCV in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study through immunological and molecular methods.Methods The blood samples were obtained from 10520 enrolled participant. Complete biochemical and hematological assessments plus urine analysis were done. The presence of HBsAg, anti-HBs, anti-HBc and anti-HCV antibodies for all participants and HBeAg and anti-HBe antibody for HBV positive patients were evaluated. HBV genomic DNA and HCV genomic RNA were extracted from positive serum samples. Real time PCR assay was done to quantify HBV and HCV genomes. HCV genotyping was also performed.Results Most of our participants were female (53.5%), rural (56.1%), married (97.2%) with primary education (72.1%) without smoking (75.2%) or alcohol consumption (85.3%). The HBV and HCV prevalence was 0.24% (95% CI, 0.16% to 0.35%) and 0.11% (95% CI, 0.06% to 0.19%), respectively. Rural participants were significantly more HBV positive than urban peoples (P=0.045) while male individuals were significantly more HCV positive than female participants (P=0.013).Conclusion Our detected HBV and HCV prevalence were lower than other cities/provinces of Iran, which may be due to the lifestyle or other unknown reasons.

scholarly journals Epidemiologic profile of viral hepatitis B and C in North of Iran: Results from PERSIAN Guilan Cohort Study (PGCS)

2020 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Mohammadreza Naghipour ◽  
Soheil Hassanipour ◽  
Sara Yeganeh ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Urine Analysis ◽  
Health Impact ◽  
Serum Samples ◽  
Hcv Prevalence ◽  
Viral Hepatitis B ◽  
Epidemiologic Profile ◽  
North Of Iran ◽  
Hcv Genotyping

Abstract Objective: Hepatitis B (HBV) and C (HCV) viruses are two severe infectious diseases with a high global health impact. This study aimed to evaluate the prevalence of HBV and HCV in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study through immunological and molecular methods. Results: The blood samples were obtained from 10520 enrolled participants. Complete biochemical and hematological assessments plus urine analysis were done. The presence of HBsAg, anti-HBs, anti-HBc, and anti-HCV antibodies for all participants and HBeAg and anti-HBe antibody for HBV positive patients were evaluated. HBV genomic DNA and HCV genomic RNA were extracted from positive serum samples. The real-time PCR assay was done to quantify HBV and HCV genomes. HCV genotyping was also performed. The HBV and HCV prevalence was 0.24% (95% CI, 0.16 % to 0.35%) and 0.11% (95% CI, 0.06 % to 0.19%), respectively. Rural participants were significantly more HBV positive than urban peoples (P=0.045) while male individuals were significantly more HCV positive than female participants (P=0.013). Our detected HBV and HCV prevalence were lower than other cities/provinces of Iran, which may be due to lifestyle or other unknown reasons.

scholarly journals Epidemiologic profile of viral hepatitis B and C in North of Iran: Results from PERSIAN Guilan Cohort Study (PGCS)

2020 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Mohammadreza Naghipour ◽  
Soheil Hassanipour ◽  
Sara Yeganeh ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Urine Analysis ◽  
Health Impact ◽  
Serum Samples ◽  
Hcv Prevalence ◽  
Viral Hepatitis B ◽  
Epidemiologic Profile ◽  
North Of Iran ◽  
Hcv Genotyping

Abstract Objective Hepatitis B (HBV) and C (HCV) viruses are two severe infectious diseases with a high global health impact. This study aimed to evaluate the prevalence of HBV and HCV in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study through immunological and molecular methods. Results The blood samples were obtained from 10520 enrolled participants. Complete biochemical and hematological assessments plus urine analysis were done. The presence of HBsAg, anti-HBs, anti-HBc, and anti-HCV antibodies for all participants and HBeAg and anti-HBe antibody for HBV positive patients were evaluated. HBV genomic DNA and HCV genomic RNA were extracted from positive serum samples. The real-time PCR assay was done to quantify HBV and HCV genomes. HCV genotyping was also performed. The HBV and HCV prevalence was 0.24% (95% CI, 0.16% to 0.35%) and 0.11% (95% CI, 0.06% to 0.19%), respectively. Rural participants were significantly more HBV positive than urban peoples (P = 0.045) while male individuals were significantly more HCV positive than female participants (P = 0.013). Our detected HBV and HCV prevalence were lower than other cities/provinces of Iran, which may be due to lifestyle or other unknown reasons.

scholarly journals Epidemiologic profile of viral hepatitis B and C in North of Iran: Results from PERSIAN Guilan Cohort Study (PGCS)

2021 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Mohammadreza Naghipour ◽  
Soheil Hassanipour ◽  
Sara Yeganeh ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Urine Analysis ◽  
Health Impact ◽  
Serum Samples ◽  
Viral Hepatitis B ◽  
Epidemiologic Profile ◽  
North Of Iran ◽  
Urban People ◽  
Geographical Locations

Abstract Objective: Hepatitis B (HB) and C (HC) are two severe infectious diseases with a high global health impact. This study aimed to evaluate the prevalence of HB and HC in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study through immunological and molecular methods. Results: The blood samples were obtained from 10520 enrolled participants. Complete biochemical and hematological assessments plus urine analysis were done. The presence of HBsAg, anti-HBs, anti-HBc, and anti-HCV antibodies for all participants, and HBeAg and anti-HBe antibodies for HB positive patients were evaluated. HB genomic DNA and HC genomic RNA were extracted from positive serum samples. The real-time PCR assay was done to quantify HB and HC genomes. HC genotyping was also performed. The HB and HC prevalence was 0.24% (95% CI, 0.16 % to 0.35%) and 0.11% (95% CI, 0.06 % to 0.19%), respectively. Rural participants were significantly more HB positive than urban people (P=0.045), while male individuals were significantly more HC positive than female participants (P=0.013). The obtained showed the prevalence of HB and HC in this area were lower than other geographical locations of Iran, which may be due to different lifestyles or other unknown reasons.

scholarly journals Epidemiologic profile of viral hepatitis B and C in North of Iran: results from PERSIAN Guilan Cohort Study (PGCS)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Mohammadreza Naghipour ◽  
Soheil Hassanipour ◽  
Sara Yeganeh ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Urine Analysis ◽  
Serum Samples ◽  
Gene Copies ◽  
Epidemiologic Profile ◽  
North Of Iran ◽  
Urban People ◽  
Geographical Locations ◽  
Hematological Tests

Abstract Objective Hepatitis B (HB) and C (HC) are two severe viral infectious diseases with a deleterious impact on global health. This study aimed to evaluate the prevalence of HB and HC in the Prospective Epidemiological Research Studies of the Iranian Adults (PERSIAN) Guilan Cohort Study using immunological and molecular methods. Results The blood samples were obtained from 10,520 enrolled participants. Complete biochemical and hematological tests, as well as urine analysis, were assessed. The presence of HBsAg, anti-HBs, anti-HBc, and anti-HCV antibodies for all participant and HBeAg and anti-HBe antibodies for HB-positive patients were evaluated. Moreover, HB genomic DNA and HC genomic RNA were extracted from serum samples of HB-positive patients. The real-time PCR assay was employed to quantify the gene copies of hepatitis B and C viruses. HC genotyping was also performed. The prevalence of HB and HC was 0.24% (95% CI 0.16–0.35) and 0.11% (95% CI 0.06–0.19), respectively. Rural participants were significantly more HB-positive than the urban people (P = 0.045), while males were significantly more HC-positive than the females (P = 0.013). The prevalence of HB and HC in this area were lower than those of other geographical locations of Iran, which may be due to different lifestyles or other unknown reasons.

scholarly journals Sensitivity and specificity of commercially available rapid diagnostic tests for viral hepatitis B and C screening in serum samples

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0235036
Author(s):  
Ganbolor Jargalsaikhan ◽  
Miriam Eichner ◽  
Delgerbat Boldbaatar ◽  
Purevjargal Bat-Ulzii ◽  
Oyungerel Lkhagva-Ochir ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Rapid Diagnostic Tests ◽  
Serum Samples ◽  
Viral Hepatitis B

scholarly journals Clinical epidemiology of chronic viral hepatitis B: A Tuscany real-word large-scale cohort study

2018 ◽  
Vol 10 (5) ◽  
pp. 409-416 ◽  
Author(s):  
Cristina Stasi ◽  
Caterina Silvestri ◽  
Roberto Berni ◽  
Maurizia Rossana Brunetto ◽  
Anna Linda Zignego ◽  
...  
Keyword(s):  
Cohort Study ◽  
Hepatitis B ◽  
Viral Hepatitis ◽  
Large Scale ◽  
Clinical Epidemiology ◽  
Chronic Viral Hepatitis ◽  
Real Word ◽  
Viral Hepatitis B

scholarly journals Prevalence of viral hepatitis B and C in Sierra Leone—current knowledge and knowledge gaps: a narrative review

2021 ◽  
Author(s):  
Sulaiman Lakoh ◽  
Noemí García-Tardón ◽  
Olukemi Adekanmbi ◽  
Marc van der Valk ◽  
Samuel J Smith ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Sierra Leone ◽  
Blood Donors ◽  
Current Knowledge ◽  
School Age Children ◽  
Knowledge Gaps ◽  
Hcv Prevalence ◽  
Viral Hepatitis B ◽  
Control And Prevention

Abstract There are no comprehensive data on viral hepatitis from Sierra Leone; however, a huge disease burden has been observed in different subpopulations. This review summarizes available data on hepatitis B and C virus (HBV and HCV) prevalence in Sierra Leone and identifies knowledge gaps. Despite the non-uniformity of the studies and the lack of systematic case recording, different reports published in recent decades yielded a hepatitis B prevalence of 8.7% among healthcare workers, 11.3% among pregnant women, 15.2% among blood donors and 16.7% in school-age children. The actual HBV prevalence in the general population was reported as 21.7%; similar to what was reported for people living with human immunodeficiency virus (PLHIV). HCV prevalence is 8% and 7% in male and female blood donors, respectively, 4.1% in PLHIV and 2.0% in school children. There are significant knowledge gaps regarding the prevalence of viral hepatitis B and C in Sierra Leone, despite the high burden reported in a few studies. There are limited programmatic interventions on the control and prevention of viral hepatitis in the country. Therefore, well-structured representative studies should provide a solid understanding of the true prevalence of hepatitis B and C to inform best possible public health measures in Sierra Leone.

scholarly journals Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay

2016 ◽  
Vol 54 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Hui Wang ◽  
Xiaohui Bi ◽  
Lei Xu ◽  
Yirong Li
Keyword(s):  
Hepatitis B ◽  
Rheumatoid Factor ◽  
Hepatitis B Surface Antigen ◽  
Alpha Fetoprotein ◽  
Serum Samples ◽  
Negative Interference ◽  
Positive Serum ◽  
Serum Alpha Fetoprotein ◽  
Chemiluminescent Microparticle Immunoassay ◽  
Paramagnetic Microparticles

Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen–anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime, laboratorians must remain alert to the negative interference by rheumatoid factor, and in some cases, pretreat rheumatoid factor-positive samples with blocking or absorbing reagents.

scholarly journals Clinical Evaluation of a New PCR Assay for Detection of Coxiella burnetii in Human Serum Samples

1998 ◽  
Vol 36 (1) ◽  
pp. 77-80 ◽  
Author(s):  
G. Q. Zhang ◽  
Sa V. Nguyen ◽  
H. To ◽  
M. Ogawa ◽  
A. Hotta ◽  
...  
Keyword(s):  
Human Serum ◽  
Coxiella Burnetii ◽  
Nested Pcr ◽  
Restriction Enzymes ◽  
Pcr Assay ◽  
Serum Samples ◽  
Gene Encoding ◽  
Human Serum Samples ◽  
Positive Serum ◽  
Pcr Method

A nested PCR method was developed for the detection ofCoxiella burnetii in human serum samples. Two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kDa outer membrane protein ofC. burnetii. The primers amplified the predicted fragments of 21 various strains of C. burnetii but did not react with DNA samples from other microorganisms. The 438-bp amplification products could be digested with restriction enzymes SspI and SalI. The utility of the nested PCR was evaluated by testing human serum samples. The com1 gene fragment was amplified from 135 (87%) of 155 indirect immunofluorescence test (IF)-positive serum samples and from 11 (11%) of 100 IF-negative serum samples. The nested PCR with primers targeted to the com1gene appeared to be a sensitive, specific, and useful method for the detection of C. burnetii in serum samples.

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