Calcium phosphate influences erosivity of non alcoholic beverages in vitro

Abstract Background: The effect of added calcium phosphate to non alcoholic beverages to reduce erosivity on bovine enamel and dentine was analyzed. Methods: 90 enamel and 90 dentine specimens from freshly extracted bovine incisors were randomly attributed to 9 enamel and 9 dentine groups (n=10 each). Initially, all specimens were weighted using a precision balance. They were suspended in plastic bottles containing 150ml of the following beverages: Cola, orange juice, Red Bull, Bonaqua Fruits. To all beverages 100mg (c1) or 200mg (c2) calcium hydrogen phosphate dihydrate was added. Tap water served as control. After 7 days, median mass loss was calculated in mg. Kruskal-Wallis and Mann-Whitney u-test served for comparison between groups. Results: Cola and Bonaqua Fruits lost erosivity already after addition of 100mg calcium phosphate. Red Bull after 100mg on enamel and 200mg on dentine. Only orange juice remained erosive on dentine and enamel even after the addition of 200mg calcium phosphate. Conclusion: The addition of calcium phosphate may reduce or even prevent the erosivity of some highly erosive non alcoholic beverages on bovine enamel and dentine.

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Effect of CPP-ACP Application on Flexural Strength of Bleached Enamel and Dentin Complex

Operative Dentistry ◽

10.2341/10-280-l ◽

2011 ◽

Vol 36 (4) ◽

pp. 372-379 ◽

Cited By ~ 14

Author(s):

M Khoroushi ◽

H Mazaheri ◽

AE Manoochehri

Keyword(s):

Calcium Phosphate ◽

Flexural Strength ◽

Amorphous Calcium Phosphate ◽

Clinical Relevance ◽

In Vitro Simulation ◽

Casein Phosphopeptide ◽

Bovine Enamel ◽

At Home

Clinical Relevance In the absence of saliva, flexural strength of the bovine enamel-dentin complex decreases after in vitro simulation of both at-home and in-office bleaching procedures. Application of Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) subsequent to both bleaching regimens could compensate for this decrease.

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Preparation and evaluation of an ispaghula based directly compressible matrixing agent for controlled release

Acta Pharmaceutica ◽

10.2478/v10007-008-0015-2 ◽

2008 ◽

Vol 58 (3) ◽

pp. 309-316 ◽

Cited By ~ 1

Author(s):

Anita Lalwani ◽

Jolly Parikh

Keyword(s):

Controlled Release ◽

Hydroxypropyl Methylcellulose ◽

Hydrogen Phosphate ◽

Lattice Design ◽

Phosphate Dihydrate ◽

Simplex Lattice Design ◽

Dependent Variables ◽

Simplex Lattice ◽

Preparation And Evaluation

Preparation and evaluation of an ispaghula based directly compressible matrixing agent for controlled releaseThe objective of the present investigation was to prepare and evaluate an ispaghula husk based directly compressible (DC) adjuvant that can be used as matrixing agent using an agglomeration technique. Addition of hydroxypropyl methylcellulose was found necessary to improve cohesion. Lactose (X1), calcium hydrogen phosphate dihydrate (X2) and Avicel PH101 (X3), used along with ispaghula in preparation of agglomerates, were selected as three independent variables in a simplex lattice design affecting compressional and dissolution characteristics of the drug from the DC adjuvant. The agglomerates were evaluated for their flow properties. Tablets were prepared using 70% agglomerates and 30% acetaminophen, a poorly compressible drug, and were subjected toin vitrodrug release study. Amounts of the drug released at the end of 60 min (Y60), 300 min (Y300) and 480 min (Y480) were selected as dependent variables in a simplex lattice design. Batch IH05 that contained lactose and calcium hydrogen phosphate dihydrate in a 1:2 ratio could control the release for 12 hours and thus form the basis for twice a-day-dosing.

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In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins

Dental Materials ◽

10.1016/j.dental.2020.07.007 ◽

2020 ◽

Vol 36 (10) ◽

pp. 1343-1355 ◽

Cited By ~ 3

Author(s):

Wen Zhou ◽

Xian Peng ◽

Xuedong Zhou ◽

Michael D. Weir ◽

Mary Anne S. Melo ◽

...

Keyword(s):

Calcium Phosphate ◽

In Vitro Evaluation ◽

Calcium Phosphate Nanoparticles ◽

Bovine Enamel

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Study on Hydroxyapatite Fibers with Strontium Reinforced Calcium Phosphate Cement

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.788.119 ◽

2013 ◽

Vol 788 ◽

pp. 119-126 ◽

Cited By ~ 1

Author(s):

Bin Chu ◽

Jian Xiong ◽

Ming Bo Wang ◽

Xiao Li Li ◽

Zhen Ding She

Keyword(s):

Mechanical Property ◽

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Solidification Time ◽

Cytotoxicity Test ◽

Dicalcium Phosphate Dihydrate ◽

Phosphate Dihydrate ◽

Main Components ◽

Sem Morphology

The solidification time of injectable bone cement should be fit for the clinical application. This research find out the effect of the amount of water-absorbent agent and water-retaining agent. The optimal ratio was be determined. The hydroxyapatite fibers with strontium were added into the CPC. The mechanical property, cytotoxicity test, SEM morphology, XRD and degradation performance in vitro were characterized, respectively. The results show the CPC had the solidification time of 12min when the ratio as below: β-TCP 55.5%, Ca (H2PO4)2H2O(MCPM)36%, MgHPO43H2O 5%, MgSO41%, Sodium pyrophosphate2.5%. The main components of solidify CPC were hydroxyapatite (HA) and dicalcium phosphate dihydrate (DCPD). The materials had a good Anti-collapsing performance and the degradation rate up to 16.72% after 9 weeks. The mechanical property of composite materials which combined with hydroxyapatite fibers with strontium has been improved, and the cell proliferation rate is also higher than common CPC. This study shows a potentially effective method to improve the mechanical property and the biological activity of calcium phosphate cement.

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Phase transition of amorphous calcium phosphate to calcium hydrogen phosphate dihydrate in simulated body fluid

Journal of Crystal Growth ◽

10.1016/j.jcrysgro.2020.125937 ◽

2021 ◽

Vol 553 ◽

pp. 125937

Author(s):

Hiroki Saito ◽

Yuki Araki ◽

Hiroyasu Katsuno ◽

Toshitaka Nakada

Keyword(s):

Phase Transition ◽

Calcium Phosphate ◽

Simulated Body Fluid ◽

Amorphous Calcium Phosphate ◽

Body Fluid ◽

Hydrogen Phosphate ◽

Phosphate Dihydrate

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Impact of Experimental Nano-HAP Pastes on Bovine Enamel and Dentin Submitted to a pH Cycling Model

Brazilian Dental Journal ◽

10.1590/0103-6440201302175 ◽

2013 ◽

Vol 24 (3) ◽

pp. 273-278 ◽

Cited By ~ 13

Author(s):

Livia P. Comar ◽

Beatriz M. Souza ◽

Luiz Fernando Gracindo ◽

Marilia A. R. Buzalaf ◽

Ana Carolina Magalhaes

Keyword(s):

Calcium Phosphate ◽

Repeated Measures ◽

Surface Hardness ◽

In Vitro Study ◽

Cross Sectional ◽

Before And After ◽

Mi Paste ◽

Ph Cycling ◽

Bovine Enamel

This in vitro study evaluated the preventive potential of experimental pastes containing 10% and 20% hydroxyapatite nanoparticles (Nano-HAP), with or without fluoride, on dental demineralization. Bovine enamel (n=15) and root dentin (n=15) specimens were divided into 9 groups according to their surface hardness: control (without treatment), 20 Nanop paste (20% HAP), 20 Nanop paste plus (20% HAP + 0.2% NaF), 10 Nanop paste (10% HAP), 10 Nanop paste plus (10% HAP + 0.2% NaF), placebo paste (without fluoride and HAP), fluoride paste (0.2% NaF), MI paste (CPP-ACP, casein phosphopeptide-amorphous calcium phosphate), and MI paste plus (CPP-ACP + 0.2% NaF). Both MI pastes were included as commercial control products containing calcium phosphate. The specimens were treated with the pastes twice a day (1 min), before and after demineralization. The specimens were subjected to a pH-cycling model (demineralization–6-8 h/ remineralization-16-18 h a day) for 7 days. The dental subsurface demineralization was analyzed using cross-sectional hardness (kgf/mm 2 , depth 10-220 µm). Data were tested using repeated-measures two-way ANOVA and Bonferroni's test (p<0.05). The only treatment able to reduce the loss of enamel and dentin subsurface hardness was fluoride paste (0.2% NaF), which differed significantly from the control at 30- and 50-µm depth (p<0.0001). The other treatments were not different from each other or compared with the control. The experimental Nanop pastes, regardless of the addition of fluoride, were unable to reduce dental demineralization in vitro.

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An Aqueous Extract of the Leaves of Ficus religiosa Inhibits the Growth of Urinary Calcium Hydrogen Phosphate Dihydrate Crystals-An In-Vitro Study

Alternative Complementary & Integrative Medicine ◽

10.24966/acim-7562/100041 ◽

2017 ◽

Vol 3 (4) ◽

pp. 1-6

Author(s):

Jayakumar T ◽

Keyword(s):

Aqueous Extract ◽

In Vitro Study ◽

Urinary Calcium ◽

Vitro Study ◽

Hydrogen Phosphate ◽

Ficus Religiosa ◽

Phosphate Dihydrate

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IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

Journal of industrial technology ◽

10.21908/jit.2015.8 ◽

2015 ◽

Vol 23 (1) ◽

pp. 1-14

Author(s):

Sudirman Sahid ◽

◽

Nor Shahida Kader Bashah ◽

Salina Sabudin ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Biphasic Calcium Phosphate ◽

Silica Content

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Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

BMC Oral Health ◽

10.1186/s12903-021-01548-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Philipp Körner ◽

Luca Georgis ◽

Daniel B. Wiedemeier ◽

Thomas Attin ◽

Florian J. Wegehaupt

Keyword(s):

Gastroesophageal Reflux ◽

Artificial Saliva ◽

In Vitro Study ◽

Tooth Wear ◽

Control Group ◽

Erosive Tooth Wear ◽

Custom Made ◽

Post Hoc ◽

Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

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