scholarly journals Early-life Psychological Stress Leads to Impaired Spatial Learning and Memory and Alters Hippocampal Proteome in Adult Rats

2021 ◽  
Author(s):  
Lin Han ◽  
Xiaofan Xiong ◽  
Meiyang Fan ◽  
Lingyu Zhang ◽  
Liying Liu ◽  
...  
Keyword(s):  
Protein Expression ◽  
Learning And Memory ◽  
Psychological Stress ◽  
Spatial Learning ◽  
Early Life ◽  
Adult Stage ◽  
Early Stage ◽  
The Other ◽  
Spatial Learning And Memory ◽  
Adult Rats

Abstract Early-life psychological stress (ELPS) can cause anxiety, pessimism, and a decrease of cognitive ability in adult individuals. In this study, a psychological stress model (a terrified sound stress) was applied to new-born Sprague-Dawley rats for 21 days. And then, we separately evaluated the impact of ELPS on their spatial learning and memory abilities and hippocampal proteome from early-stage to the adult-stage. The Morris Water Maze (MWM) test was employed to evaluate their spatial learning and memory abilities after ELPS till to the adult-stage. Two-dimensional gel electrophoresis (2DE) as well as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to uncover the protein expression profile of the hippocampus from both ELPS-young and ELPS-adult as well as their control groups. We found that the rats had a dysfunction of spatial learning and memory after the ELPS till to the adult-stage. The proteomic analysis revealed that 51 proteins were significant differentially expression, and 25 of them were down-regulated, while the other 26 proteins were up-regulated in the hippocampus of the ELPS-young rats compared with the controls. In the ELPS-adult rats, there were 56 significant differentially expression proteins, and 42 of them were down-regulated, the other 14 proteins were up-regulated in the hippocampus compared with their controls. Thirteen of the most significant differentially expressed proteins in ELPS-adult hippocampus were identified as SPTAN1, MYH4, HSPA8, HS90A, DYN1, DLDH, ARP3, GLNA, SAHH, HBB1, ACLY, TBB2A and GBB1, that demonstrated the greatest stress-induced changes. Furthermore, western blotting analyses consistently showed that the reduced expression of SPTAN1 and MYH4 whereas the expression of HSPA8 was up-regulated in the hippocampus after ELPS till to the adult-stage. The current study showed the impaired spatial learning and memory and changed hippocampal gene expressions induced by ELPS from early-stage to adult-stage in rats. This study shows that ELPS plays an important role in behavioral cognition and hippocampal protein expression in adult rats.

Sex- and age-dependent effects of early postnatal sibling deprivation on spatial learning and memory in adult rats

2008 ◽  
Vol 186 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Yan-Qin Li ◽  
Xiao-yi Wang ◽  
Hai-feng Zhai ◽  
Xiang Yang Zhang ◽  
Therese Kosten ◽  
...  
Keyword(s):  
Learning And Memory ◽  
Spatial Learning ◽  
Spatial Learning And Memory ◽  
Adult Rats ◽  
Age Dependent

scholarly journals Effect of Short- and Long-Term Administration of Baclofen on Spatial Learning and Memory in Rats

2018 ◽  
pp. 133-141 ◽  
Author(s):  
M. HOLAJOVA ◽  
M. FRANEK
Keyword(s):  
Learning And Memory ◽  
Spatial Learning ◽  
Receptor Agonist ◽  
Gabab Receptor ◽  
Spatial Learning And Memory ◽  
Adult Rats ◽  
Treatment Groups ◽  
Term Administration ◽  
Short And Long Term

Baclofen is the only clinically available metabotropic GABAB receptor agonist. In our experiment, we tested the hypothesis that long-term baclofen administration can impair learning and memory in rats. The experiment consisted of three parts. In the first part of the study the drug was administered simultaneously with the beginning of the behavioral tests. In the second and third part of the experiment baclofen was administered daily for 14 days and for one month before the tests. In each part of the experiment, adult rats were randomly divided into four treatment groups. Three groups were given an injection of baclofen at doses of 1 mg/kg, 5 mg/kg, 10 mg/kg, while the fourth group was injected with saline. The injections were given after each session. Spatial learning and memory were tested using the Morris water maze, involving three types of tests: Acquisition, Probe, and Re-acquisition. This work reveals that baclofen did not affect spatial learning at any of the tested doses and regardless of the length of administration. Memory was observed to be affected, but only at the highest dose of baclofen and only temporarily. This conclusion is in line with previously published clinical cases.

Ameliorating Ribosylation-Induced Amyloid-β Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling

2020 ◽  
pp. 1-12
Author(s):  
Yang-Yang Wang ◽  
Qian Yan ◽  
Zhen-Ting Huang ◽  
Qian Zou ◽  
Jing Li ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Learning And Memory ◽  
Spatial Learning ◽  
Early Stage ◽  
Amyloid Β ◽  
Mtor Signaling ◽  
Immunofluorescence Staining ◽  
Morris Water Maze Test ◽  
Spatial Learning And Memory ◽  
Aβ Production

Background: Berberine (BBR) plays a neuroprotective role in the pathogenesis of Alzheimer’s disease (AD), inhibiting amyloid-β (Aβ) production and promoting Aβ clearance. Advanced glycation end products (AGEs) promote Aβ aggregation and tau hyperphosphorylation. The activation of mTOR signaling occurring at the early stage of AD has a prominent impact on the Aβ production. This work focused on whether BBR regulates the production and clearance of ribosylation-induced Aβ pathology via inhibiting mTOR signaling. Objective: To explore whether BBR ameliorates ribosylation-induced Aβ pathology in APP/PS1 mice. Methods: Western blot and immunofluorescence staining were used to detect the related proteins of the mammalian target of Rapamycin (mTOR) signaling pathway and autophagy, as well as the related kinases of Aβ generation and clearance. Tissue sections and Immunofluorescence staining were used to observe Aβ42 in APP/PS1 mice hippocampal. Morris water maze test was used to measure the spatial learning and memory of APP/PS1 mice. Results: BBR improves spatial learning and memory of APP/PS1 mice. BBR limits the activation of mTOR/p70S6K signaling pathway and enhances autophagy process. BBR reduces the activity of BACE1 and γ-secretase induced by D-ribose, and enhances Aβ-degrading enzymes and Neprilysin, and inhibits the expression of Aβ in APP/PS1 mice. Conclusion: BBR ameliorates ribosylation-induced Aβ pathology via inhibiting mTOR/p70S6K signaling and improves spatial learning and memory of the APP/PS1 mice.

scholarly journals Ameliorating Ribosylation-induced Aβ Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling

2020 ◽  
Author(s):  
Yang-Yang Wang ◽  
Qian Yan ◽  
Zhen-Ting Huang ◽  
Qian Zou ◽  
Jing Li ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Learning And Memory ◽  
Spatial Learning ◽  
Early Stage ◽  
Mammalian Target Of Rapamycin ◽  
Mtor Signaling ◽  
Immunofluorescence Staining ◽  
Spatial Learning And Memory ◽  
Aβ Generation ◽  
Aβ Production

Abstract Background: Berberine (BBR) plays a neuroprotective role in the pathogenesis of Alzheimer’s disease (AD), inhibiting β-amyloid (Aβ) production and promoting Aβ clearance. Advanced glycation end products (AGEs) promote Aβ aggregation and tau hyperphosphorylation. The activation of mTOR signaling occurring at the early stage of AD has a prominent impact on the Aβ production. This work focused on whether BBR regulates the production and clearance of ribosylation-induced Aβ pathology via inhibiting mTOR signaling.Methods: Western Blot and Immunofluorescence staining were used to detect the related proteins of the mammalian target of Rapamycin(mTOR) signaling pathway and autophagy, as well as the related kinases of Aβ generation and clearance. Tissue sections and Immunofluorescence staining were used to observe Aβ1-42 in APP/PS1 mice hippocampal. Morris water maze (MWM) test was used to measure the spatial learning and memory of APP/PS1 mice.Results: BBR improves spatial learning and memory of APP/PS1 mice. BBR limits the activation of mTOR/p70S6K signaling pathway and enhances autophagy process. BBR reduces the activity of BACE1and γ-secretase induced by D-ribose, and enhances Aβ-degrading enzymes and Neprilysin, and inhibits the expression of Aβin APP/PS1 mice.Conclusions: BBR ameliorates ribosylation-induced Aβ pathology via inhibiting mTOR/p70S6K signaling, and improves spatial learning and memory of the APP/PS1 mice.

scholarly journals Early-life stress leads to impaired spatial learning and memory in middle-aged ApoE4-TR mice

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Lan-yan Lin ◽  
Jing Zhang ◽  
Xiao-man Dai ◽  
Nai-an Xiao ◽  
Xi-lin Wu ◽  
...  
Keyword(s):  
Learning And Memory ◽  
Spatial Learning ◽  
Life Stress ◽  
Early Life ◽  
Early Life Stress ◽  
Spatial Learning And Memory ◽  
Middle Aged
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