scholarly journals Abortion storm of Yili Horses is Associated with Equus Caballus Papillomavirus 2 Variant Infection

2021 ◽  
Author(s):  
Panpan Tong ◽  
Ruli Duan ◽  
Xiaozhen Song ◽  
Nuerlan Palidan ◽  
Haifeng Deng ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Epidemiological Study ◽  
Causative Agent ◽  
Equus Caballus ◽  
Equine Arteritis Virus ◽  
Bovine Papillomavirus ◽  
Sequence Analyses ◽  
Equid Herpesvirus ◽  
Thoroughbred Horses ◽  
Nasal Swabs

Abstract Background: Nine different species of Equus caballus papillomavirus (EcPV) and three bovine papillomavirus (BPV) have been reported to infect horses, however, there are so far no describing such infections in China. In January 2021, an abortion storm occurred in Yili horses, as a result of which 50 out of 93 aborted fetus samples were found to be negative for equid herpesvirus (EHV) and equine arteritis virus (EAV).Results: In our pioneer study with Chinese horses, we first found EcPV-2 in the nasal swabs (4/230, 1.7%) of Yili horses, and semen (3/18, 16.7%) of the Thoroughbred horses. This indicated that EcPV can be indeed hosted by horses in China, and that EcPV-2 might be transmitted though breeding. Further detection of EcPVs in the lung tissues of aborted fetus in Yili horses, which were originally negative for equid herpesviruses, established that EcPV-2 was positive in 19 of 50 samples, thereby indicating that EcPV-2 might be a new pathogen causing of abortions. Thereafter, the sequence analyses for L1 genes sequences of 26 China’s EcPV-2 were performed which indicated that EcPV-2, that primarily infected the horses in China, shared 98.3%-99.9% nt identity with the already published sequences for EcPV-2. These observations indicated that EcPV-2 identified in the current study were highly similar variants of the previously identified strains of EcPV-2. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-2, found in the Chinese horses, was closely related to and clustered together with an already known EcPV-2a lineage. Conclusion: Our study provides the first evidence related to EcPV-2 infection in the Chinese horses, which can serve as a causative agent for Yili horse abortions, and thus can possibly lay the foundation for a systematic and detailed epidemiological study of this infection in the Chinese horses.

First Detection and Genetic Characterization of Equus Caballus Papillomavirus 1, 2 and 7 in China

2021 ◽  
Author(s):  
Panpan Tong ◽  
Xiaozhen Song ◽  
Meiling Ren ◽  
Erken Jia ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Epidemiological Study ◽  
Genetic Characterization ◽  
Equus Caballus ◽  
Genomic Sequences ◽  
Sequence Analyses ◽  
Pioneer Study ◽  
Nasal Swabs ◽  
Complete Genomic

Abstract Background: Nine species of Equus caballus papillomavirus (EcPV) have been reported to infect horses, however, there are so far no reports of such infections in China. Results: In our pioneer study with Chinese horses, we found EcPV-1 in intranasal papilloma and nasal swabs, EcPV-2 in nasal swabs and semen, and EcPV-7 primarily in semen. This indicates that EcPVs are indeed hosted by horses in China, and that EcPV-2 and 7 may be getting transmitted though breeding. Sequence analyses for complete genomic sequences of EcPV-1 (G2), EcPV-2 (XJ-KS1391) and EcPV-7 (XJ-zs1) were performed which indicated that EcPV-1, 2 and 7, that infect horses in China, share 99.3% nt identity with the already published sequences for EcPV-1, 2 and 7. These observations indicate that three types of EcPVs identified in the current study are highly similar variants of previously known types of EcPV-1, 2 and 7. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-1, 2 and 7, found in Chinese horses, are closely related to and clustered together with already known EcPV-1, 2 and 7, respectively. Conclusion: Our study provides a novel evidence for EcPVs infection and circulation in Chinese horses and thus lays the foundation for a systematic and detailed epidemiological study of these infections in Chinese horses.

Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland

2011 ◽  
Vol 14 (4) ◽  
Author(s):  
A. Szczerba-Turek ◽  
J. Siemionek ◽  
A. Bancerz-Kisiel ◽  
A. Raś ◽  
W. Szweda
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Papillomavirus

scholarly journals Vanderbylia cinnamomea sp. nov. from southwestern China

Mycotaxon ◽  
2020 ◽  
Vol 135 (2) ◽  
pp. 371-382
Author(s):  
Jun-Zhu Chen ◽  
Xiong Yang ◽  
Chang-Lin Zhao
Keyword(s):  
Phylogenetic Analysis ◽  
Morphological Characters ◽  
Southwestern China ◽  
Pore Surface ◽  
Sequence Analyses ◽  
Hyphal System ◽  
High Support

Vanderbylia cinnamomea is proposed as a new wood-inhabiting polypore species based on morphological characters supported by phylogenetic analysis. The species is characterized by an annual, pileate basidiocarp, a brown to black pileal surface, an olivaceous buff to smoke grey pore surface, a dimitic hyphal system with clamped generative hyphae, and subglobose to amygdaliform, hyaline, thick-walled, smooth, dextrinoid, and cyanophilous basidiospores. ITS+nLSU sequence analyses place V. cinnamomea in Vanderbylia within a subclade with a high support (100% BS, 100% BP, 1.00 BPP) sister to a subclade comprising V. fraxinea and V. robiniophila.

Phylogenetic placement of Bahusandhika, Cancellidium and Pseudoepicoccum (asexual Ascomycota)

Phytotaxa ◽  
2014 ◽  
Vol 176 (1) ◽  
pp. 68 ◽  
Author(s):  
J. PRATIBHA ◽  
A. PRABHUGAONKAR ◽  
K.D. HYDE ◽  
D.J. Bhat
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequence ◽  
Type Species ◽  
Reference Data ◽  
Sequence Data ◽  
Sequence Analyses ◽  
Natural Classification ◽  
Phylogenetic Placement ◽  
Close Relationship ◽  
Distinct Lineage

Most hyphomycetous conidial fungi cannot be presently placed in a natural classification. They need recollecting and sequencing so that phylogenetic analysis can resolve their taxonomic affinities. The type species of the asexual genera, Bahusandhika, Cancellidium and Pseudoepicoccum were recollected, isolated in culture, and the ITS and LSU gene regions sequenced. The sequence data were analysed with reference data obtained through GenBank. The DNA sequence analyses shows that Bahusandhika indica has a close relationship with Berkleasmium in the order Pleosporales and Pseudoepicoccum cocos with Piedraia in Capnodiales; both are members of Dothideomycetes. Cancellidium applanatum forms a distinct lineage in the Sordariomycetes.

scholarly journals Phylogenetic analysis of a partial L1 gene from bovine papillomavirus type 1 isolated from naturally occurring papilloma cases in the northwestern region of Turkey

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
M. Ozkan Timurkan ◽  
M. Eray Alcigir
Keyword(s):  
Phylogenetic Analysis ◽  
Histopathological Examination ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Sequencing Analysis ◽  
Molecular Characterisation ◽  
Northwestern Turkey ◽  
Northwestern Region

This study was aimed at the molecular characterisation of bovine papillomavirus type 1 (BPV-1) isolated from papilloma cases in the northwestern region of Turkey. BPV-1 is a widely occurring oncogenic virus in cattle and is associated with benign epithelial neoplasia which causes significant economic losses in dairy and beef cattle because of treatment costs. In this study, 29 suspected papilloma specimens were collected from cattle in northwestern Turkey. These samples underwent molecular characterisation via the polymerase chain reaction (PCR) and sequencing analysis as well as macroscopic and histopathological examination. The histopathological examinations confirmed papilloma as the main lesion type in the specimens. Of the 29 papilloma-like tissue samples that were collected, 11 (i.e. 37.93%) were detected as positive and determined as containing BPV-1 (11 of 11, 100%). Using a partial sequence for the L1 gene acquired from GenBank, phylogenetic analysis confirmed the presence of BPV-1 and revealed that the infection might have originated in cross bred domestic and imported cattle. This study provides potentially useful information on the origin and spread of this disease. Its results can potentially aid in the development of appropriate control measures and therapeutic or vaccination strategies against the BPV-1 strain of bovine papillomatosis.

scholarly journals Molecular characterization of the viral structural protein genes in the first outbreak of dengue virus type 2 in Hunan Province, inland China in 2018

2021 ◽  
Author(s):  
Jiaoqiong Guan ◽  
Zhanlong He ◽  
Meng Qin ◽  
Xialin Deng ◽  
Jun Chen ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Protein Binding ◽  
Causative Agent ◽  
Binding Sites ◽  
Selection Pressure ◽  
Hunan Province ◽  
Genetic Characteristics ◽  
Protein Binding Sites ◽  
Dengue Outbreak

Abstract Background: An unexpected dengue outbreak occurred in Hunan Province in 2018. This was the first dengue outbreak in this area of inland China, and 172 cases were reported.Methods: To verify the causative agent of this outbreak and characterise the viral genes, the genes encoding the structural proteins C/prM/E of viruses isolated from local residents were sequenced followed by mutation and phylogenetic analysis. Recombination, selection pressure, potential secondary structure and three-dimensional structure analyses were also performed. Results: Phylogenetic analysis revealed that all epidemic strains were of the cosmopolitan DENV-2 genotype and were most closely related to the Zhejiang strain (MH010629, 2017) and then the Malaysia strain (KJ806803, 2013). Compared with the sequence of DENV-2SS, 151 base substitutions were found in the sequences of 89 isolates; these substitutions resulted in 20 non-synonymous mutations, of which 17 mutations existed in all samples (two in the capsid protein, six in the prM/M proteins, and nine in the envelope proteins). Moreover, amino acid substitutions at the 602nd (E322:Q→H) and 670th (E390: N→S) amino acids may have enhanced the virulence of the epidemic strains. One new DNA binding site and five new protein binding sites were observed. Two polynucleotide binding sites and seven protein binding sites were lost in the epidemic strains compared with DENV-2SS. Meanwhile, five changes were found in helical regions. Minor changes were observed in helical transmembrane and disordered regions. The 429th amino acid of the E protein switched from a histamine (positively charged) to an asparagine (neutral) in all 89 isolated strains. No recombination events or positive selection pressure sites were observed. To our knowledge, this study is the first to analyse the genetic characteristics of epidemic strains in the first dengue outbreak in Hunan Province in inland China.Conclusions: The causative agent is likely to come from Zhejiang Province, a neighbouring province where dengue fever broke out in 2017. This study may help clarify the intrinsic geographical relatedness of DENV-2 and contribute to further research on pathogenicity and vaccine development.

scholarly journals Serological investigation of equine viral arteritis in donkeys in eastern and south-eastern Anatolia regions of Turkey

2019 ◽  
Vol 88 (4) ◽  
pp. 385-391 ◽  
Author(s):  
Sibel Gür ◽  
Bünyamin İrehan ◽  
Metin Gürçay ◽  
Turhan Turan
Keyword(s):  
Study Data ◽  
Equine Arteritis Virus ◽  
Family Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Serum Samples ◽  
Equine Viral Arteritis ◽  
South Eastern ◽  
Thoroughbred Horses ◽  
First Time

Equine arteritis virus is classified in the Arteriviridae family and causes reproductive and respiratory disorders. The host spectrum includes many species of the Equidae family. Horses, donkeys and mules are the most sensitive species. The infection was serologically investigated in adult donkeys on small private family type enterprises in eastern and south-eastern Anatolia in this study. A total of 1,532 samples were collected from 28 different locations in 6 different provinces in these two regions. The number of donkeys sampled from each farm was between 1 and 3. Serum samples were tested by indirect enzyme-linked immunosorbent assay (ELISA). As a result of sero-controls, 53 animals were positive (3.45%). The presence of infection was determined in all the provinces; Elazığ (7%, 17/241), Tunceli (2.4%, 3/122), Van (2.9%, 10/342), Bitlis (4.6%, 5/107), Şırnak (2.7%, 12/440) and Siirt (2.1%, 6/280). Seropositivity was detected in 22 of the 28 locations. In this study, data were obtained from a significant number of animals for the first time in these regions. Although the values were not high, the findings revealed the presence of infection in the majority of the investigated sites. Despite the fact that the incidence was not high in donkeys probably due to restricted management conditions, the incidence may increase over time and may pose a risk for thoroughbred horses unless necessary measures are taken.

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