scholarly journals Comparison of Telomere Length Between Buccal Cells and Blood Cells

2021 ◽  
Author(s):  
Lu Xu ◽  
Zhuoziqi Qiu ◽  
Yu-Sheng Cong
Keyword(s):  
Telomere Length ◽  
Blood Cells ◽  
Buccal Cell ◽  
Strong Positive Correlation ◽  
Buccal Cells ◽  
Qpcr Method ◽  
Adequate Quality ◽  
Polymerase Chain ◽  
Length Analysis ◽  
Biomarker Of Aging

Abstract Background Telomere length (TL) in blood has been extensively studied as a biomarker of aging and aging-associated disease. TL in blood cells is commonly used as a proxy for TL in other tissue types. The source of DNA of adequate quality and quantity is an important consideration in telomere length analysis. Compared to blood cells, buccal cells easy for genomic DNA preparation would facilitate the rapid and reliable telomere length analysis. However, the feasibility of buccal cells for TL analysis remains yet unestablished. Methods A total of 52 participants ranged in age from 18 to 80 years including 24 males and 28 females were included in this study. Both buccal and blood samples were taken at the same time by using buccal cell swabs and fingertip stick from each participant. Relative telomere length (RTL) was analyzed using the quantitative real-time polymerase chain reaction (qPCR) method. Results The results indicate that there is a strong positive correlation between buccal RTL and blood RTL and negative correlation between both buccal RTL and blood RTL with age. Conclusion The validity of sampling using buccal cell swabs provides simple operation and good reproducibility for telomere length analysis, which overcomes the discomfort and risk of infection caused by blood sampling.

scholarly journals Validation of quantitative polymerase chain reaction with Southern blot method for telomere length analysis

2018 ◽  
Vol 4 (4) ◽  
pp. FSO282 ◽  
Author(s):  
Mohamad Tarik ◽  
Lakshmy Ramakrishnan ◽  
Harshpal S Sachdev ◽  
Nikhil Tandon ◽  
Ambuj Roy ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Telomere Length ◽  
Southern Blot ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Length Analysis

Telomere length analysis on cord blood cells by the flow-FISH method

Haematologia ◽  
2002 ◽  
Vol 32 (3) ◽  
pp. 265-269 ◽  
Author(s):  
Nóra Regéczy ◽  
Sándor Valent ◽  
Luca Kormos ◽  
Katalin Pálóczi ◽  
Melinda Hajdu ◽  
...  
Keyword(s):  
Cord Blood ◽  
Telomere Length ◽  
Blood Cells ◽  
Fish Method ◽  
Cord Blood Cells ◽  
Length Analysis

scholarly journals The Association between 25-Hydroxyvitamin D Concentration and Telomere Length in the Very-Old: The Newcastle 85+ Study

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4341
Author(s):  
Sarah Hakeem ◽  
Nuno Mendonça ◽  
Terry Aspray ◽  
Andrew Kingston ◽  
Carmen Martin-Ruiz ◽  
...  
Keyword(s):  
Telomere Length ◽  
Blood Cells ◽  
Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Cross Sectional ◽  
Very Old ◽  
Old Adults ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Polymerase Chain

(1) Introduction: vitamin D may maintain the telomere length, either directly or via the inflammation effect and/or modulating the rate of cell proliferation. Whilst results from cross-sectional studies investigating the association between 25(OH)D concentration and telomere length have been mixed, there is a dearth of data from prospective studies which have assessed these associations. This study aimed to examine the association between 25(OH)D concentration in plasma and telomere length in blood cells in very-old adults (≥85 years old) at baseline, 18 months and 36 months by controlling for related lifestyle factors. (2) Methodology: our prospective cohort study comprised 775 participants from the Newcastle 85+ Study who had 25(OH)D measurements at baseline. Plasma 25(OH)D was stratified as <25 nmol/L (low), 25–50 nmol/L (moderate) and >50 nmol/L (high). Peripheral blood mononuclear cell telomere length was measured by quantitative real-time polymerase chain reaction at baseline, 18 and 36 months from baseline. (3) Results: a positive significant association was found between 25(OH)D concentration and telomere length amongst very-old participants at baseline (95% CI = 12.0–110.3, B = 61.2 ± 5.0, p = 0.015). This association was negative at 18 months (95% CI = −59.9–−7.5, B = −33.7 ± 13.3, p = 0.012) but was non-significant at 36 months. (4) Conclusion: Circulating 25(OH)D concentration shows inconsistent relationships with telomere length over time in very-old (85+ year old) adults.

Comparison of Telomere Length in Young and Master Endurance Runners and Sprinters

2021 ◽  
pp. 1-7
Author(s):  
Matt Nickels ◽  
Sarabjit Mastana ◽  
Veryan Codd ◽  
Matthew Denniff ◽  
Elizabeth Akam
Keyword(s):  
Body Mass Index ◽  
Telomere Length ◽  
Quantitative Polymerase Chain Reaction ◽  
Buccal Cell ◽  
Lower Body ◽  
Master Athletes ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Endurance Runners ◽  
Single Laboratory

It is unclear how running modality influences telomere length (TL). This single laboratory visit study compared the TL of master sprinters and endurance runners with their young counterparts. The correlation between leukocyte and buccal cell TL in athletes was also explored. Participants consisted of 11 young controls, 11 young sprinters, 12 young endurance runners, 12 middle-aged controls, 11 master sprinters, and 12 master endurance runners. Blood and buccal samples were collected and randomized for analysis of TL by quantitative polymerase chain reaction. Young endurance runners displayed longer telomeres than master athletes (p < .05); however, these differences were not significant when controlled for covariates (p > .05). A positive correlation existed between leukocyte and buccal cell TL in athletes (r = .567, p < .001). In conclusion, young endurance runners possess longer telomeres than master endurance runners and sprinters, a consequence of lower body mass index and visceral fat.

scholarly journals Cytokinesis-block micronucleus cytome (CBMN-CYT) assay biomarkers and telomere length analysis in relation to inorganic elements in individuals exposed to welding fumes

2021 ◽  
Vol 212 ◽  
pp. 111935
Author(s):  
Milton Quintana-Sosa ◽  
Grethel León-Mejía ◽  
Jaime Luna-Carrascal ◽  
Yurina Sh De moya ◽  
Ibeth Luna Rodríguez ◽  
...  
Keyword(s):  
Telomere Length ◽  
Welding Fumes ◽  
Inorganic Elements ◽  
Length Analysis
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