Immobilization of Glucanobacter Xylinum Onto Natural Polymers to Enhance the Bacterial Cellulose Productivity

Abstract Bacterial cellulose (BC) has profound applications in different sectors of biotechnology due to its unique properties preferring it about plant cellulose. Although this polymer is extremely important in various applications, many problems still hinder the sustainable production in terms of increasing productivity and low-cost production. In order to overcome these problems, this study will focuses on the continuous production of cellulose using immobilized Glucanobacter xylinum cells onto Sugar Cane Bagasse (SCB) and Ca-alginate beads. Comparatively, adsorption of Glucanobacter xylinum cells to the cavum of stalk cells of SCB could be efficiently stable while, entrapment of cells onto Ca-alginate has drawback observed by the rapid disruption and instability of the beads in the Potato Peel Waste (PPW) culture medium. Our findings demonstrate that a combination between alternative low-cost medium with continuous production mode by immobilization onto inexpensive natural polymer can promote a sustainable bioprocess and reduction the production cost.

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Kluyveromyces lactis cells entrapped in Ca-alginate beads for the continuous production of a heterologous glucoamylase

Journal of Biotechnology ◽

10.1016/j.jbiotec.2003.10.028 ◽

2004 ◽

Vol 109 (1-2) ◽

pp. 83-92 ◽

Cited By ~ 14

Author(s):

Elisabetta de Alteriis ◽

Giovanni Silvestro ◽

Massimo Poletto ◽

Vittorio Romano ◽

Daniele Capitanio ◽

...

Keyword(s):

Kluyveromyces Lactis ◽

Continuous Production ◽

Alginate Beads ◽

Ca Alginate

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Novel synthesis strategies for natural polymer and composite biomaterials as potential scaffolds for tissue engineering

Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rsta.2010.0009 ◽

2010 ◽

Vol 368 (1917) ◽

pp. 1981-1997 ◽

Cited By ~ 66

Author(s):

Hsu-Feng Ko ◽

Charles Sfeir ◽

Prashant N. Kumta

Keyword(s):

Tissue Engineering ◽

Low Cost ◽

Three Dimensional ◽

Natural Polymer ◽

Natural Polymers ◽

Porous Network ◽

Tissue Engineering Scaffolds ◽

Composite Scaffolds ◽

Polymeric Scaffold ◽

Recent Developments

Recent developments in tissue engineering approaches frequently revolve around the use of three-dimensional scaffolds to function as the template for cellular activities to repair, rebuild and regenerate damaged or lost tissues. While there are several biomaterials to select as three-dimensional scaffolds, it is generally agreed that a biomaterial to be used in tissue engineering needs to possess certain material characteristics such as biocompatibility, suitable surface chemistry, interconnected porosity, desired mechanical properties and biodegradability. The use of naturally derived polymers as three-dimensional scaffolds has been gaining widespread attention owing to their favourable attributes of biocompatibility, low cost and ease of processing. This paper discusses the synthesis of various polysaccharide-based, naturally derived polymers, and the potential of using these biomaterials to serve as tissue engineering three-dimensional scaffolds is also evaluated. In this study, naturally derived polymers, specifically cellulose, chitosan, alginate and agarose, and their composites, are examined. Single-component scaffolds of plain cellulose, plain chitosan and plain alginate as well as composite scaffolds of cellulose–alginate, cellulose–agarose, cellulose–chitosan, chitosan–alginate and chitosan–agarose are synthesized, and their suitability as tissue engineering scaffolds is assessed. It is shown that naturally derived polymers in the form of hydrogels can be synthesized, and the lyophilization technique is used to synthesize various composites comprising these natural polymers. The composite scaffolds appear to be sponge-like after lyophilization. Scanning electron microscopy is used to demonstrate the formation of an interconnected porous network within the polymeric scaffold following lyophilization. It is also established that HeLa cells attach and proliferate well on scaffolds of cellulose, chitosan or alginate. The synthesis protocols reported in this study can therefore be used to manufacture naturally derived polymer-based scaffolds as potential biomaterials for various tissue engineering applications.

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A review on introduction and applications of starch and its biodegradable polymers

International Journal of Environment ◽

10.3126/ije.v4i4.14108 ◽

2015 ◽

Vol 4 (4) ◽

pp. 114-125 ◽

Cited By ~ 2

Author(s):

Shanta Pokhrel

Keyword(s):

Structural Properties ◽

Biodegradable Polymers ◽

Low Cost ◽

Synthetic Polymers ◽

High Availability ◽

Natural Polymer ◽

Natural Polymers ◽

Potential Applications

Biodegradable polymers play a very important role in plastic engineering by replacing non biodegradable, non renewable petrol based polymers. Starch is a renewable, biodegradable, low cost natural polymer with high availability. Natural polymers can be blended with synthetic polymers to improve their properties significantly. This article reviews advance in starch and starch based blends and presents their numerous potential applications. Therefore, this review helps to understand the importance and characteristics of starch and its biodegradable polymers (blends) by its various aspects such as structural properties and wide applications.International Journal of Environment Vol.4(4) 2015: 114-125

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Incorporation of micro/nanoparticles of PCL with essential oil of Cymbopogon nardus in bacterial cellulose

International Journal of Advances in Medical Biotechnology - IJAMB ◽

10.25061/2595-3931/ijamb/2018.v1i2.18 ◽

2018 ◽

Vol 1 (2) ◽

pp. 37

Author(s):

Aline Krindges ◽

Vanusca Dalosto Jahno ◽

Fernando Morisso

Keyword(s):

Particle Size ◽

Particulate Matter ◽

Essential Oil ◽

Zeta Potential ◽

Bacterial Cellulose ◽

Culture Medium ◽

Static Culture ◽

Cymbopogon Nardus ◽

Cellulose Membranes

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.

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Characterization and Screening of a Novel Multiparticulate Pulsatile Delivery of Aceclofenac

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2016.9.5.7 ◽

2016 ◽

Vol 9 (5) ◽

pp. 3494-3501

Author(s):

Bipul Nath ◽

Santimoni Saikia

Keyword(s):

Drug Release ◽

Sodium Alginate ◽

Alginate Beads ◽

In Vitro Drug Release ◽

Dsc Studies ◽

Ft Ir ◽

Lag Period ◽

Pulsatile Delivery ◽

Ca Alginate

In the present investigation, sodium alginate based multiparticulate system overcoated with time and pH dependent polymer was studied in the form of oral pulsatile system to achieve pulsatile with sustained release of aceclofenac for chronotherapy of rheumatoid arthritis seven batches of micro beads with varying concentration of sodium alginate (2-5 %) were prepared by ionotropic-gelation method using CaCl2 as cross-linking agent. The prepared Ca-alginate beads were coated with 5% Eudragit L100 and filled into pulsatile capsule with varying proportion of plugging materials. Drug loaded microbeads were investigated for physicochemical properties and drug release characteristics. The mean particle sizes of drug-loaded microbeads were found to be in the range 596±1.1 to 860 ± 1.2 micron and %DEE in the range of 65-85%. FT-IR and DSC studies revealed the absence of drug polymer interactions. The release of aceclofenac from formulations F1 to F7 in buffer media (pH 6.8) at the end of 5h was 65.6, 60.7, 55.7, 41.2, 39.2, 27 and 25% respectively. Pulsatile system filled with eudragit coated Ca-alginate microbeads (F2) showed better drug content, particle size, surface topography, in-vitro drug release in a controlled manner. Different plugging materials like Sterculia gum, HPMC K4M and Carbopol were used in the design of pulsatile capsule. The pulsatile system remained intact in buffer pH 1.2 for 2 hours due to enteric coat of the system with HPMCP. The enteric coat dissolved when the pH of medium was changed to 7.4. The pulsatile system developed with Sterculia gum as plugging material showed satisfactory lag period when compared to HPMC and Carbopol.

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Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization

Biochemical Engineering Journal ◽

10.1016/j.bej.2021.108058 ◽

2021 ◽

pp. 108058

Author(s):

Talita Corrêa Nazareth ◽

Conrado Planas Zanutto ◽

Danielle Maass ◽

Antônio Augusto Ulson de Souza ◽

Selene Maria de Arruda Guelli Ulson de Souza

Keyword(s):

Culture Medium ◽

Medium Optimization ◽

Low Cost ◽

Culture Medium Optimization ◽

Surfactin Production

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Preparation and Antimicrobial Activity of Chitosan and Its Derivatives: A Concise Review

Molecules ◽

10.3390/molecules26123694 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3694

Author(s):

Luminita Georgeta Confederat ◽

Cristina Gabriela Tuchilus ◽

Maria Dragan ◽

Mousa Sha’at ◽

Oana Maria Dragostin

Keyword(s):

Antimicrobial Activity ◽

Low Cost ◽

Hydroxyl Groups ◽

Pharmacological Properties ◽

Natural Polymers ◽

Economic Sectors ◽

Physico Chemical ◽

Long Time ◽

Resistant Strains ◽

Strength And Durability

Despite the advantages presented by synthetic polymers such as strength and durability, the lack of biodegradability associated with the persistence in the environment for a long time turned the attention of researchers to natural polymers. Being biodegradable, biopolymers proved to be extremely beneficial to the environment. At present, they represent an important class of materials with applications in all economic sectors, but also in medicine. They find applications as absorbers, cosmetics, controlled drug delivery, tissue engineering, etc. Chitosan is one of the natural polymers which raised a strong interest for researchers due to some exceptional properties such as biodegradability, biocompatibility, nontoxicity, non-antigenicity, low-cost and numerous pharmacological properties as antimicrobial, antitumor, antioxidant, antidiabetic, immunoenhancing. In addition to this, the free amino and hydroxyl groups make it susceptible to a series of structural modulations, obtaining some derivatives with different biomedical applications. This review approaches the physico-chemical and pharmacological properties of chitosan and its derivatives, focusing on the antimicrobial potential including mechanism of action, factors that influence the antimicrobial activity and the activity against resistant strains, topics of great interest in the context of the concern raised by the available therapeutic options for infections, especially with resistant strains.

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Biosynthesis of Carboxymethylated Bacterial Cellulose Composite for Wound Dressing

Materials Science Forum ◽

10.4028/www.scientific.net/msf.685.322 ◽

2011 ◽

Vol 685 ◽

pp. 322-326 ◽

Cited By ~ 5

Author(s):

Jun Wei Yu ◽

Xiao Li Liu ◽

Chang Sheng Liu ◽

Dong Ping Sun

Keyword(s):

Bacterial Cellulose ◽

Wound Dressing ◽

Culture Medium ◽

Acetobacter Xylinum ◽

Water Holding Capacity ◽

Water Soluble ◽

Transmission Rates ◽

Measurement Results ◽

Water Holding ◽

Cellulose Composite

A novel bacterial cellulose (BC) composite (carboxymethylated-bacterial cellulose, CM-BC) was synthesized by Acetobacter xylinum by adding water-soluble carboxymethylated cellulose (CMC) in the culture medium. FTIR results showed that CM-BC is obtained by the incorporation of CMC in the network of BC. Water-holding capacity and water vapor transmission rates (WVTR) of CM-BC and BC are determined. The WVTR of CM-BC is comparable to that of BC, but the water-holding capacity of CM-BC is improved compared with BC. Tensile strengths measurement results showed that the fracture stress of CM-BC is higher than that of BC, indicating that CM-BC have more potential wound dressing applications than BC.

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