Study on the Mechanism of Gastrodia Rhizoma "FEATURE Identification based Quality Assessmen" based on Neuroprotection
Abstract Background: The theory of "Feature Identification based Quality Assessmen" is the essence of traditional Chinese medicine experience identification, and the theory is to identify the quality of Chinese Materia Medica by its properties. The mechanism of evaluating quality through "Feature " has not been clarified. Methods: We used artificial intelligence sensory technology electronic nose, electronic tongue and other instruments to quantitatively determine the “feature” include "Shape, Color, Qi, taste" of Gastrodia Rhizoma. The relationship between fingerprint of chemical constituents and protective effects on OGD/R injury of SH-SY5Y cells of 30 batches of medicinal materials was analyzed, and the Pharmacodynamic Components Group (six compounds) of Gastrodia Rhizoma were determined. In vitro and in vivo pharmacodynamic experiments confirmed that the Pharmacodynamic Components Group had good protective effects on OGD/R injury of SH-SY5Y cells, spinal injury of zebrafish and cerebral ischemia of rats, and could be widely distributed in rats.Results: The Pharmacodynamic Components Group could represent more than 90% of the whole pharmacodynamic effect of Gastrodia Rhizoma, which showed that the Pharmacodynamic Components Group was basically equivalent to the crude drug. Through the correlation analysis of “feature” and Pharmacodynamic Components Group, we revealed that the mechanism of “Feature Identification based Quality Assessmen” of Gastrodia Rhizoma was related to the six components of the Pharmacodynamic Components Group. Conclusions: The mechanism of "Feature Identification based Quality Assessmen" of Gastrodia Rhizoma based on "Feature" is that "Shape", "Color", "Qi" and "Taste" of Gastrodia Rhizoma have significant correlation with the content of Pharmacodynamic Components Group. This study provides a new way of thinking for the interpretation of the scientific connotation of " Feature Identification based Quality Assessmen" and the quality evaluation of traditional Chinese medicine