scholarly journals HPLC and high-throughput sequencing revealed differences of tea-leaves quality components and soil microbial community structure between modern and ancient tea plantations in Lincang Region

2022 ◽  
Author(s):  
Guangrong Yang ◽  
Dapeng Zhou ◽  
Renyuan Wan ◽  
Conglian Wang ◽  
Jin Xie ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Soil Fertility ◽  
High Throughput Sequencing ◽  
Chemical Components ◽  
Tea Leaves ◽  
Tea Plantation ◽  
Soil Microbial ◽  
Tea Plantations ◽  
Abundance And Diversity

Abstract BackgroundAncient tea plantations with an age over 100 years still reserved at Mengku Town in Lincang Region of Yunan Province, China. However, the characteristic of soil chemicophysical properties and microbial ecosystem in the ancient tea plantations and their impact on tea-leaves chemical components remained unclear. Tea-leaves chemical components including amino acids, phenolic compounds and purine alkaloids, and soil chemicophysical properties including pH, cation exchange capacity (CEC), soil organic matter (SOM), soil organic carbon (SOC), total total nitrogen (TN), total phosphorus (TP), total potassium (TK), alkali-hydrolyzable nitrogen (AN), available phosphorous (AP) and available potassium (AK), and microbial community structure of modern and ancient tea plantations in five geographic sites (i.e. Bingdao, Baqishan, Banuo, Dongguo and Jiulong) were determined by high performance liquid chromatography (HPLC) and high-throughput sequencing, respectively. ResultsTea-leaves chemical components, soil chemicophysical properties and microbial community structures including bacterial and fungal community abundance and diversity evaluated by Chao 1 and Shannon varied with geographic location and tea plantation type. The ancient tea plantations possessed significantly (P<0.05) higher free amino acids, gallic acid, caffeine and EGC in tea-leaves, as well as soil fertility. The bacterial community structure kept stable, while fungal community abundance and diversity significantly (P<0.05) increased in ancient tea plantation because of higher soil fertility and lower pH. The long-term plantation in natural cultivation way significantly (P<0.05) improved the abundances of Nitrospirota, Methylomirabilota, Ascomycota and Mortierellomycota phyla. ConclusionsDue to the natural cultivation way, the ancient tea plantations still maintained relatively higher soil fertility and complete soil microbial ecosystem, which contributed to the sustainable development with higher quality in tea-leaves.

scholarly journals Soil Microbial Community Variation With Time and Soil Depth in Eurasian Steppe (Inner Mongolia, China)

2021 ◽  
Author(s):  
Hongbin Zhao ◽  
Wenling Zheng ◽  
Shengwei Zhang ◽  
Wenlong Gao ◽  
Yueyue Fan
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Environmental Factors ◽  
Soil Microbial Community ◽  
Soil Depth ◽  
Microbial Community Composition ◽  
Spatial Transformation ◽  
Soil Microbial ◽  
Abundance And Diversity ◽  
Bacteria And Fungi

Abstract BackgroundSoil microorganisms play an indispensable role in the material and energy cycle of grassland ecosystem, and were affected by many environmental factors, such as time and space changes. However, there are few studies on the temporal and spatial transformation of soil microbial community in typical degraded steppe. We analyzed the community structure and diversity of soil bacteria and fungi and the effects of environmental factors on the community structure in Xilingol degraded steppe. ResultsThe abundance and diversity of bacteria and fungi were significantly affected by depth. Bacteria and fungi diversity of 10 cm was higher than that of 20 cm and 30 cm. The abundance of Acidobacteria, Proteobacteria, Actinomycetes, Ascomycetes and Basidiomycetes varies significantly with depth. What’s more, soil pH increased significantly with depth increasing, while SOM, AN, VWC and ST decreased significantly with increasing depth. In addition, Depth, TOC and AN had significant impact on the bacterial and fungi communities (p < 0.05). ConclusionsSpatial heterogeneity (depth) is more important than temporal (month) in predicting changes in microbial community composition and soil properties. And the abundance of Acidobacteria, Proteobacteria, Actinomycetes, Ascomycetes and Basidiomycetes varies significantly with depth. We speculate that SOM and VWC account for the abundance variations of Acidobacteria and Proteobacteria, and pH cause the abundance changes of Actinomycetes, Ascomycetes and Basidiomycota.

scholarly journals Effects of Freezing-Thawing on Microbial Community Structure in the Soil of Old Apple Orchards

HortScience ◽  
2021 ◽  
pp. 1-7
Author(s):  
Haiyan Wang ◽  
Rong Zhang ◽  
Weitao Jiang ◽  
Yunfei Mao ◽  
Xuesen Chen ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Alpha Diversity ◽  
Apple Orchards ◽  
Soil Layers ◽  
Soil Microbial ◽  
Average Temperature ◽  
Abundance And Diversity ◽  
The Relationship

The study here aimed to investigate the effects of pre-winter ditching and freezing-thawing on soil microbial structure in different soil layers of old apple orchards. A total of 30 samples were obtained from 3 Nov. 2016 to 9 Mar. 2017. The relative abundance, alpha diversity, community structure of fungi, and the relationship between environmental factors and microbial community structure were analyzed, and the greenhouse experiments were used for further verification. Results showed that the number of actinomycete and total bacterial colonies decreased, whereas the number of fungi sustained decreased, resulting in a higher bacteria/fungi ratio. The percentage of Fusarium initially decreased, then later increased by 11.38%, 3.469%, 2.35%, 2.29%, and 3.09%. However, Fusarium levels were still 9% lower on 9 Mar. 2017 that on 3 Nov. 2016. Both the abundance and diversity of the community were higher in the upper soil than in the lower. The main environmental factor contributing to the percentage of Fusarium change was average temperature (AT), although highest temperature (HT) and water content (WC) also had an impact. The Malus hupehensis Rehd. seedlings growing in lower soil were more vigorous than that in upper soil. In sum, pre-winter ditching and freezing-thawing in old apple orchards can reduce the abundance percentage of harmful Fusarium and promote the growth of M. hupehensis Rehd. seedlings.

scholarly journals Disentangling Responses of the Subsurface Microbiome to Wetland Status and Implications for Indicating Ecosystem Functions

2021 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Jie Gao ◽  
Miao Liu ◽  
Sixue Shi ◽  
Ying Liu ◽  
Yu Duan ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Carbon Fixation ◽  
Microbial Community Composition ◽  
Ecosystem Functions ◽  
Microbial Composition ◽  
Wetland Ecosystems ◽  
Soil Microbial ◽  
Geochemical Properties ◽  
Microbial Groups

In this study, we analyzed microbial community composition and the functional capacities of degraded sites and restored/natural sites in two typical wetlands of Northeast China—the Phragmites marsh and the Carex marsh, respectively. The degradation of these wetlands, caused by grazing or land drainage for irrigation, alters microbial community components and functional structures, in addition to changing the aboveground vegetation and soil geochemical properties. Bacterial and fungal diversity at the degraded sites were significantly lower than those at restored/natural sites, indicating that soil microbial groups were sensitive to disturbances in wetland ecosystems. Further, a combined analysis using high-throughput sequencing and GeoChip arrays showed that the abundance of carbon fixation and degradation, and ~95% genes involved in nitrogen cycling were increased in abundance at grazed Phragmites sites, likely due to the stimulating impact of urine and dung deposition. In contrast, the abundance of genes involved in methane cycling was significantly increased in restored wetlands. Particularly, we found that microbial composition and activity gradually shifts according to the hierarchical marsh sites. Altogether, this study demonstrated that microbial communities as a whole could respond to wetland changes and revealed the functional potential of microbes in regulating biogeochemical cycles.

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