scholarly journals Histopathological Features and Immunophenotype of Primary Gastric Invasive Fibromatosis

2020 ◽  
Author(s):  
Yangkun Wang ◽  
Zhishang Zhang ◽  
Bo Jiang ◽  
Chaoya Zhu ◽  
Sunan Wang ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Tumor Tissue ◽  
Gastric Wall ◽  
Clinical Manifestations ◽  
Nerve Tissue ◽  
Proliferation Index ◽  
Fatty Tissue ◽  
Ki 67 ◽  
Positive Expression ◽  
S 100

Abstract Background To investigate the histopathological characteristics, immunophenotype and differential diagnosis of primary gastric invasive fibromatosis.Methods The clinical manifestations, histological morphology and immunophenotype of 4 cases of primary gastric invasive fibromatosis were observed and related literatures were reviewed.Results Among the 4 patients, 2 were males and 2 were females, aged 28 and 47 years, respectively. The lesions were located in the stomach in 2 cases, the gastric antrum in 1 case and cardia in 1 case. Under the microscope, the tumor was located in the submucosa, growing infiltratingly, and infiltrating into the gastric wall muscularis, serous membrane and extraserous fatty tissue. Tumor cells were rich in cytoplasm, with unclear cell boundaries, long rod-shaped nuclei, deep chromatin, no atypia, and rare mitotic figures. The tumor tissue was composed of proliferating spindle-shaped fibroblasts and collagen fibers, and the cell morphology was relatively uniform, arranged in parallel bundles or staggered weaves. Tumor tissue invaded and destroyed smooth muscle, blood vessels, nerve tissue and adipose tissue of the gastric wall. Immunophenotype: positive expression of vimentin, β-catenin, SMA positive expression; CKpan, EMA, S-100 protein, desmin, CD99, Bcl-2, ALK, CD34, CD117, DOG1, hormone receptors were all negative; The cell proliferation index ki-67 positive cells were 3–5%.Conclusion Primary invasive fibromatosis of the stomach is a relatively rare spindle cell tumor, which needs to be differentiated from tumors and pathological lesions such as inflammatory myofibroblastic tumor, plexiform mucinous fibroma, gastrointestinal stromal tumor, etc.

scholarly journals A Rare Case of Aggressive Systemic Mastocytosis Involving the Gastrointestinal System and Review of Literature

2021 ◽  
Author(s):  
Jianjun Wang ◽  
Zhong Zheng ◽  
Feng-nan Niu ◽  
Ting Wang ◽  
Biao Zhang ◽  
...  
Keyword(s):  
Mast Cells ◽  
Tumor Cells ◽  
Systemic Mastocytosis ◽  
Clinical Manifestations ◽  
Skin Lesions ◽  
Proliferation Index ◽  
Pelvic Cavity ◽  
Ki 67 ◽  
S 100 ◽  
Hepatic Portal

Abstract Background Systemic mastocytosis is a rare disease and most patients have pigmented urticarial skin lesions. It can be easily missed and misdiagnosed in small biopsies, especially in those patients with nonspecific clinical complaints or untypical skin lesions. Case presentation We report a case of 69-year-old man who have presented with 2-year of diarrhea, progressive weight loss of 20kg and abdominal distention for 3 months. Ultrasound and abdominal CT scan showed massive effusions in abdominal and pelvic cavity. Colonoscopy was performed and showed intensive mucosal proliferations forming polypoid appearances. Microscopically, monotonously uniform, small, round tumor cells with slightly rich cytoplasm were concentrated between the residual glands in the colonoscopic biopsy. The tumor cells showed positive expression of CD117 and S-100, and low Ki-67 proliferation index of 2%, while Trypsin, CK, CD68, CD1a, Langerin, Syn, CgA, CD56, SATB2, CD20, CD3, α-inhibin and SMA were all negative. KIT D816V mutation was detected as well. Liver biopsy showed that CD117 positive cells were more than 15 cells in aggregates around the hepatic portal area and less than 15% mast cells were found in bone marrow smears. No multiple or diffuse pattern of mast cells infiltration was seen by repeated skin biopsies of skin lesions. With all these considered, the diagnosis of aggressive systemic mastocytosis was made. Conclusions The diagnosis of aggressive systemic mastocytosis is challenging for untypical clinical manifestations and subtle or inconspicuous lesions, especially in endoscopic biopsies, which requires awareness and a close teamwork of pathologists and clinicians.

NUCLEAR LOCALIZATION OF CRABP1 IN NEUROENDOCRINE LUNG TUMORS IS ASSOCIATED WITH THE DEGREE OF TUMOR MALIGNANCY

2017 ◽  
Vol 63 (6) ◽  
pp. 886-893
Author(s):  
Vera Delektorskaya ◽  
Andrey Komelkov ◽  
Irina Zborovskaya ◽  
Yelena Chevkina ◽  
A. Yenikeev ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Clinical Manifestations ◽  
Tumor Grade ◽  
Large Cell ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Ki 67 ◽  
Disease Prognosis ◽  
Neuroendocrine Lung Tumors ◽  
Relationship Of

Bronchopulmonary neuroendocrine tumors (NETs) refer to malignant epithelial neoplasms of neuroendocrine origin, which form highly heterogeneous group with respect to biological behavior and clinical manifestations. Three main categories of different grades of malignancy are distinguished in the diagnosis of lung NETs: typical carcinoids (TK), atypical carcinoids (AK) and the most aggressive low-differentiated tumors including small-cell and large-cell neuroendocrine lung carcinomas. These groups differ in terms of disease prognosis and therapeutic approaches, but the criteria currently used do not always allow clear boundaries between different histological variants. The search for additional diagnostic parameters and individual prognosis markers is currently actual for the grading and optimal classification of NETs. For the first time we studied the expression of Retinoic Acid Binding Protein-1 (CRABP1) in different variants of lung NETs. IHC analysis of 43 samples of lung NETs with various degrees of differentiation and grades revealed the statistically significant correlation between nuclear localization of CRABP1 and proliferation index «Ki-67» and tumor grade. The results pointed on the involvement of CRABP1 in the pathogenesis of lung NETs and indicated the need for further investigation of the relationship of the nuclear CRABP1 with clinical parameters and patient survival to determine whether this protein can be used as a marker for differential diagnosis and/or disease prognosis.

Predictors of invasive breast cancer or DCIS recurrence in estrogen receptor positive (ER+) and estrogen receptor negative (ER-) ductal carcinoma in situ (DCIS) patients with and without associated invasive carcinoma (IC)

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e11523-e11523 ◽  
Author(s):  
J. Picarsic ◽  
A. Brufsky ◽  
A. Onisko ◽  
M. Chivukula
Keyword(s):  
Estrogen Receptor ◽  
Biological Markers ◽  
Hormone Receptors ◽  
Invasive Carcinoma ◽  
Ductal Carcinoma ◽  
Response To Therapy ◽  
Proliferation Index ◽  
Low Grade ◽  
Ki 67 ◽  
Mib 1

e11523 Background: DCIS is a heterogeneous pre-invasive carcinoma with a spectrum of clinical behavior. Patients with ER+ IC have better outcomes compared to ER- patients. FOXA1 and GATA 3 family of transcription factors have been shown to be associated with hormone receptors (ER and PR) and other variables of good prognosis with better overall and relapse-free survival rate. The specific aim of this study is to analyze the expression of these novel biological markers: FOXA1, GATA-3, with recognized markers: MIB-1(Ki-67) and HER2 /neu in DCIS patients with/without associated IC. Methods: Sixty-nine (69) cases of DCIS [(fifty two (52) cases in ER+; seventeen (17) in ER-] were retrieved from our Pathology database. The expressions of the biological markers are analyzed by using a panel of immunohistochemical stains. FOXA1, GATA 3, ER, PR are nuclear stains, a cumulative “H score” is derived based on proportionality (PS) and intensity scores (IS). A proliferation index (PI) is calculated for MIB-1 (Ki67) nuclear stain (low <10%, moderate 11–25%, high 26–50%, very high>50%). Her2/neu is scored as per guidelines for HercepTest (0, or 1+ =negative, 2+ =weakly positive, 3+ =strongly positive). Results: DCIS is categorized into low grade (LG) (nuclear grade 1 and 2), high grade (HG) (grade 3). In the HGDCIS (n=48), four (4) cases had IC after a mean of 7.75 years; three cases of recurrent DCIS after a mean 6 years. No recurrent IC or DCIS is seen in the LGDCIS (n=21) group. The results are shown in the Table . Conclusions: (1) Decreased expression of GATA 3 is observed in HGDCIS ER- group may be a contributor to higher recurrence observed in this group (14%) versus (0%) in ER+ group. (2) A strong expression of FOXA, GATA3, low Ki-67 index, absent Her 2 expression are characteristically seen in our ER+ DCIS group, as previously described in IC. 3. Comparing the response to therapy and outcome in the ER+ and ER- groups is on going. [Table: see text] No significant financial relationships to disclose.

scholarly journals Gastric GIST with progressive mitotic index

2021 ◽  
Vol 17 (1) ◽  
pp. 46-51
Author(s):  
Costel Bradea ◽  
Cristian Lupascu ◽  
Valentina Munteanu ◽  
Delia Ciobanu ◽  
Catalina Cucu ◽  
...  
Keyword(s):  
Mitotic Index ◽  
Surgical Resection ◽  
Abdominal Cavity ◽  
Gastric Wall ◽  
Clinical Manifestations ◽  
Gastric Gist ◽  
Imatinib Treatment ◽  
Ki 67 ◽  
Chemical Examination ◽  
Average Survival

Gastrointestinal stromal tumors (GISTs) account for less than 1% of gastrointestinal tumors;they are the most common mesenchymal neoplasms of the gastrointestinal(GI) tract. GISTs are usually located in the stomach,but can occur anywhere along the gastrointestinal tract.GIST ranks third as histology after adenocarcinomas and lymphomas among the gastrointestinal tract.Case presentation.The patient, aged 58 years, is transferred from the Gastroenterology Clinic with symptoms of stenosis and hemorrhage from a endophytic submucosal tumor located on the posterior gastric wall, under the eso-gastric junction, objectified endoscopically and CT scan. Laparoscopic wedge resection was performed with three linear Endo-GIA staplers. The evolution was favorable. After 7 months (without chemotherapy because pTNM was T1NoMo), the patient is sent back by the gastroenterologist for „CT scan:perigastric lymphadenopathies”. The patient was operated laparoscopically converted.We found 10 tumors with typical malignant GISTs 3-10 cm diameter,on the peritoneal serosa:in the right subhepatic space, perigastric, left interhepato-phrenic, hepato-gastric;these were excised R0; with simple evolution. The immuno-histo-chemical examination specifies the diagnosis of GIST at the first and the second operation (DOG1,CD117,CD34-positively in tumor).Ki 67 was 15% in the tumor and the mitotic index <5/5 square mm at the moment of the first operation but in the metastatic tumor at the reintervention Ki 67 was 80% and mitotic index >5/5 square mm.Discutions.GIST tumours can be classified into low-risk and high-risk categories of recurrence depending on size, location, capsule rupture and mitotic activity. Disseminated metastases in the abdominal cavity are the most common clinical manifestations of malignancy. Complete surgical resection is recommended if bleeding or other symptoms are present. Tumour perforation, spontaneous or produced at the time of surgical resection, should be recorded because it has a high negative prognostic value due to peritoneal contamination. The average survival rate in localized disease is 5 years but in metastatic or recurrent disease is about 10-20 months. Conclusion. Complete excision of residual metastatic lesions has been shown to be associated with a favourable prognosis, provided that the patient responds to imatinib treatment; resection of tumour recurrence is accompanied by an average survival of 15 months. Gastric GIST recurrence risk depends of localisation, tumor size, mitotic index and capsular rupture.

scholarly journals Nfatc1 and Tumor Associated Macrophages Affect Progression of Certain Subsets of Diffuse Large B Cell Lymphoma Non-Gcb Subtypes

2021 ◽  
Vol 5 (2) ◽  
pp. 345-353
Author(s):  
Krisna Murti ◽  
Muslina Muslina ◽  
Ika Kartika ◽  
Rachmat Hidayat ◽  
Ella Amalia
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Therapeutic Strategies ◽  
Proliferation Index ◽  
Ki 67 ◽  
Large B Cell Lymphoma ◽  
Positive Expression ◽  
Activated T Cell ◽  
Large B Cell

A B S T R A C TIntroduction Diffuse large B cell lymphoma (DLBCL) is the most common type ofnon-Hodgkin lymphoma among B cell lymphomas. The interaction of tumor cellswith their microenvironment (tumor microenvironment, TME) leads to progressivityof malignancy. CD163 + macrophages known as components of TME. Nuclear factorof activated T cell (NFATc1) and MYC are important transcription factors inmalignant transformation and progression. Therapeutic strategies were fastdeveloped, nevertheless, efforts to decrease DLBCL morbidity and mortality areunsatisfied, therefore,new markers for prognosis and or therapeutic options of thepatients are necessary. This study was aimed to investigate NFATc1 expression inDLBCL and its TME. Methods: Thirty-two paraffin blocks were selected thenimmunostained for expression of NFATc1, MYC, and CD163. Clinopathologic datai.e. ages, gender, and proliferation index Ki-67 were obtained. Data was analyzedby statistics Result: Positive expression of CD163 and NFATc1 was among 55%and 45% of cases respectively. All DLBCL cases in this study were non-GCBsubtype and more patients were under 60 years (66%). Positive expression ofCD163 was higher in males (69%) and in patients under 60 years (63%). Tissuespositive for both NFATc1 and CD163 was observed higher among males andpatients under 60 years. Conclusion: NFATc1 may affect development and orprogression of certain subsets of DLBCL non-GCB subtype.

scholarly journals The clinico-pathologic profile of primary and recurrent orbital/periorbital plexiform neurofibromas (OPPN)

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258802
Author(s):  
Mohammad Alabduljabbar ◽  
Diego Strianese ◽  
Osama Al-Sheikh ◽  
Hind M. Alkatan ◽  
Hailah Al-Hussain ◽  
...  
Keyword(s):  
Alcian Blue ◽  
Smooth Muscle Actin ◽  
Neurofibromatosis Type ◽  
Proliferation Index ◽  
Diffuse Type ◽  
Ki 67 ◽  
Primary Tumors ◽  
Plexiform Neurofibromas ◽  
Recurrent Tumors ◽  
S 100

To evaluate and compare the clinical and histopathological profile of primary and recurrent orbital-periorbital plexiform neurofibromas (OPPN) in patients with neurofibromatosis type 1. We retrospectively evaluated 43 primary or recurrent neurofibroma (NF) specimens from 26 patients (2002 to 2018) at the King Khaled Eye Specialist Hospital, Saudi Arabia. Demographics, clinical presentation, and surgical intervention data were collected. Histopathological specimens were studied with hematoxylin-eosin, Alcian blue, and immunohistochemical markers; S-100, CD44, CD117, smooth muscle actin (SMA), neurofilament, and Ki-67. Of the 43 NFs specimens, 20 were primary and 23 recurrent tumors. For primary NF, the ratio of plexiform to the diffuse type was 13:7, however in recurrent tumors was 3:8 after the first recurrence, and 1:5 after multiple recurrences. Of the 17 patients with primary tumors that had paired recurrent tumors, 12/17 (70.6%) primary NFs were plexiform and 5/17 (29.4%) were diffuse. However, when tumors recurred, 13/17 tumors (76.5%) were diffuse and only 4/17 tumors (23.5%) had a plexiform pattern. The odds of a tumor having a diffuse pattern in recurrent NF was significantly higher than the plexiform pattern [OR = 7.8 (95% confidence interval 1.69:36.1) P = 0.008]. Primary plexiform NFs underwent an excision at a significantly younger age than the diffuse type. Recurrent NFs had significantly higher CD44, CD117, and neurofilament labeling (P = 0.02, P = 0.01 and P<0.001 respectively) but had significantly decreased Alcian blue, and S-100 labeling (P = 0.03, and P = 0.02 respectively) compared to primary tumors. SMA and Ki-67 proliferation index were not different between primary and recurrent NFs (P = 0.86, and P = 0.3 respectively). There appears to be a high risk for primary plexiform NFs to develop a diffuse histologic pattern when they recur. Immunohistochemical staining suggests a role of mast cells (CD117) and expression of infiltration makers (CD44) in the transformation of plexiform tumors to the diffuse phenotype.

scholarly journals HAEMANGIOPERICYTOMA

2015 ◽  
Vol 22 (03) ◽  
pp. 312-316
Author(s):  
Xiang Longquan ◽  
Henry Mwakyoma
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tumor Cells ◽  
Clinical Manifestations ◽  
Proliferation Index ◽  
High Recurrence Rate ◽  
Imaging Features ◽  
Rare Tumor ◽  
Ki 67 ◽  
Fibrous Tumor

Hemangiopericytoma (HPC) in central nervous system is a rare tumor, his tumorhas a high recurrence rate and the characteristics of extracranial metastases. Objectives:To investigate the clinicopathological features, imaging features, immunohistochemicalphenotype of haemangiopericytoma (HPC) of central nervous system. Design: Hospital basedcrossectional prospective study. Period: From 24th October to 26th October 2012. Setting: FirstPeople’s Hospital of Jining City, China. Methods: The clinical manifestations, imaging features,histopathological and immunohistochemical features were analyzed combining the review of theliterature in one case of central HPC. Results: The Gross examination revealed the size of thetumor was 5cm × 4cm× 1.5cm; the section is gray, medium soft texture, and part of the area hadcapsule. The microscopic examination showed that the tumor cells were abundant and the samesize, showing round, oval or short spindle shape. The cytoplasm was eosinophilic, and part ofit was slightly translucent. The nuclei were ovoid, and the nucleoli were inconspicuous.A lot ofcapillaries lined by endothelial cells were seen in the tumor tissue, and the blood vessels weredilated like “staghorn” in some areas. Immunohistochemistry showed that tumor cells expressedVimentin, CD34, CD99, Bcl-2, PR protein. They didn’t express EMA, SMA, and S-100 protein.The proliferation index of ki-67 is about 4%. Conclusions: The central haemangiopericytomais a rare tumor, having no specific clinical manifestations and imaging features. The finaldiagnosis requires a combination of histopathological and immunohistochemical examination,and it should be differentiated from meningioma, solitary fibrous tumor, hemangioblastoma andmesenchymal chondrosarcoma, etc.

scholarly journals Markers of Lymphocytic-Macrophagal Infiltration and Their Association With the Receptor Phenotype and Proliferative Activity of Tumor Tissue in Various Molecular-Biological Types of Endometrial Cancer

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A1026-A1026
Author(s):  
Lev M Berstein ◽  
Alexander O Ivantsov ◽  
Aglaya Iyevleva
Keyword(s):  
Endometrial Cancer ◽  
Proliferative Activity ◽  
Tumor Tissue ◽  
Practical Importance ◽  
Rank Correlation ◽  
Macrophage Infiltration ◽  
Proliferation Index ◽  
Molecular Profile ◽  
Ki 67 ◽  
Receptor Phenotype

Abstract Background and Aims: The last years were characterized by a shift from the former subdivision of endometrial cancer (EC) into two main types [1, 2] to modern molecular biological classifications of this disease [3-5]. The purpose of this investigation was an attempt to compare such prognostic indicators for EC as features of lymphocytic [6] and macrophage infiltration of tumor tissue with markers of its hormonal sensitivity (receptor phenotype) and the proliferation index Ki-67 [7], taking into account the molecular biological type of the disease. Materials and Methods: The study involved material from untreated patients with endometrial cancer (a total of 219 people). The average age of patients was close to 55-60 years. Using classification of Talhouk et al. [5] allowed to perform a search for POLE mutations, evaluate by IHC the expression of the oncoprotein p53 and MMR (mismatch-repair) proteins /MLH1, MSH2, MSH6 and PMS2/, and also identify the type of disease without a characteristic molecular profile (WCMP). The IHC method was also used to study the rate of estrogen (ER) and progesterone (PR) receptors, Ki-67 proliferative activity index, as well as the severity of macrophage-lymphocytic tissue infiltration of EC based on the analysis of the macrophage (CD68) and lymphocytic cells (cytotoxic CD8 and regulatory FoxP3) markers using reagents from Ventana and Dako. Statistical assessment of the relationships of the studied indicators was carried out by the Spearman rank correlation coefficient. Results: FoxP3 (in contrast to CD8 and CD68) positively and significantly correlates (ρ varies from 0.2895 to 0.3477) more often with ER, but not with PR. Ki-67 index in EC tissue positively and reliably correlates with FoxP3 both in the MMR-D and WCMP groups and in the combined cohort of EC patients. In the latter case, a similar relationship with Ki-67 extends to other studied markers of lymphocytic-macrophage infiltration, namely CD8 and CD68 (ρ 0,1746-0,3294). Only in the entire group of EC patients there is a positive rank correlation (0.4119!) between ER and PR expression. Conclusions: In patients with certain types of EC the connection between the estrogenic signal and PR induction is lost; it is especially noticeable in the MMR-D group, as exemplified by the negative correlation (-0.2951) of FoxP3 and PR expression. Taken together with existing data this indicates an important role of the endocrine component for differentiating separate groups of patients with EC, that may also be of practical importance. References: 1. Bokhman JV. Gynecol Oncol 1983; 15: 10-17. 2. Suarez AA et al. Gynecol Oncol 2017;144(2):243-249. 3. Murali R et al. Lancet Oncol 2014; 15: e268-278. 4.Berstein LM et al. Future Oncol. 2017 13(28):2593-2605. 5. Talhouk A. et al. Cancer. 2017;123(5):802-813. 6. Gargiulo P. et al. Cancer Treat Rev. 2016;48:61-8. 7. Kitson S. et al. Mod Pathol. 2017; 30(3): 459-468.

scholarly journals NFATc1 and Tumor Associated Macrophages Affect Progression of Certain Subsets of Diffuse Large B Cell Lymphoma Non-GCB Subtypes

2021 ◽  
Vol 5 (3) ◽  
pp. 320-328
Author(s):  
Krisna Murti ◽  
Muslina Muslina ◽  
Ika Kartika ◽  
Rachmat Hidayat ◽  
Ella Amalia
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Proliferation Index ◽  
Ki 67 ◽  
Non Hodgkin Lymphoma ◽  
Large B Cell Lymphoma ◽  
Positive Expression ◽  
Activated T Cell ◽  
Large B Cell

Introduction: Diffuse large B cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma among B cell lymphomas. The interaction of tumor cells with their microenvironment (tumor microenvironment, TME) leads to progressivity of malignancy. CD163 + macrophages known as components of TME. Nuclear factor of activated T cell (NFATc1) and MYC are important transcription factors in malignant transformation and progression. Therapeutic strategies were fast developed, nevertheless, efforts to decrease DLBCL morbidity and mortality are unsatisfied, therefore,new markers for prognosis and or therapeutic options of the patients are necessary. This study was aimed to investigate NFATc1 expression in DLBCL and its TME. Methods: Thirty-two paraffin blocks were selected then immunostained for expression of NFATc1, MYC, and CD163. Clinopathologic data i.e. ages, gender, and proliferation index Ki-67 were obtained. Data was analyzed by statistics Result: Positive expression of CD163 and NFATc1 was among 55% and 45% of cases respectively. All DLBCL cases in this study were non-GCB subtype and more patients were under 60 years (66%). Positive expression of CD163 was higher in males (69%) and in patients under 60 years (63%). Tissues positive for both NFATc1 and CD163 was observed higher among males and patients under 60 years. Conclusion: NFATc1 may affect development and or progression of certain subsets of DLBCL non-GCB subtype.

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