NUCLEAR LOCALIZATION OF CRABP1 IN NEUROENDOCRINE LUNG TUMORS IS ASSOCIATED WITH THE DEGREE OF TUMOR MALIGNANCY

2017 ◽  
Vol 63 (6) ◽  
pp. 886-893
Author(s):  
Vera Delektorskaya ◽  
Andrey Komelkov ◽  
Irina Zborovskaya ◽  
Yelena Chevkina ◽  
A. Yenikeev ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Clinical Manifestations ◽  
Tumor Grade ◽  
Large Cell ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Ki 67 ◽  
Disease Prognosis ◽  
Neuroendocrine Lung Tumors ◽  
Relationship Of

Bronchopulmonary neuroendocrine tumors (NETs) refer to malignant epithelial neoplasms of neuroendocrine origin, which form highly heterogeneous group with respect to biological behavior and clinical manifestations. Three main categories of different grades of malignancy are distinguished in the diagnosis of lung NETs: typical carcinoids (TK), atypical carcinoids (AK) and the most aggressive low-differentiated tumors including small-cell and large-cell neuroendocrine lung carcinomas. These groups differ in terms of disease prognosis and therapeutic approaches, but the criteria currently used do not always allow clear boundaries between different histological variants. The search for additional diagnostic parameters and individual prognosis markers is currently actual for the grading and optimal classification of NETs. For the first time we studied the expression of Retinoic Acid Binding Protein-1 (CRABP1) in different variants of lung NETs. IHC analysis of 43 samples of lung NETs with various degrees of differentiation and grades revealed the statistically significant correlation between nuclear localization of CRABP1 and proliferation index «Ki-67» and tumor grade. The results pointed on the involvement of CRABP1 in the pathogenesis of lung NETs and indicated the need for further investigation of the relationship of the nuclear CRABP1 with clinical parameters and patient survival to determine whether this protein can be used as a marker for differential diagnosis and/or disease prognosis.

scholarly journals Histomorphological and Immunophenotypic Characterization of Feline Injection Site-Associated Sarcoma

2021 ◽  
Vol 49 ◽  
Author(s):  
Sílvia Teixeira Pereira ◽  
Conrado Oliveira Gamba ◽  
Rodrigo Santos Horta ◽  
Rúbia Monteiro de Castro Cunha ◽  
Gleidice Eunice Lavalle ◽  
...  
Keyword(s):  
Overall Survival ◽  
Tumor Grade ◽  
Histologic Grade ◽  
Biological Behavior ◽  
Histological Subtypes ◽  
Recurrence Rates ◽  
Ki 67 ◽  
Nerve Sheath ◽  
Cox 2 ◽  
Grade Iii

Background: Feline Injection Site-Associated Sarcoma (FISS) is a mesenchymal neoplasia of aggressive behavior that develops in sites where vaccine or drugs were administered. FISS is clinically characterized by the appearance of a solitary firm nodule or a diffuse mass, adhered to tissues, in regions associated to vaccine or drug applications. Despite low prevalence, tumor recurrence rates can reach 80%. FISS present more aggressive histological characteristics when compared to sarcomas not associated to injection sites. The aim of this paper is to contribute towards the understanding of the biological behavior of FISS.Materials, Methods & Results: Sixteen samples of FISS were analyzed. Fibrosarcomas were the most frequent histological subtype (62.5%). Malignant peripheral nerve sheath tumor was diagnosed in 18.75% cases. Ten (62.5%) FISS were classified as grade II; 4/16 (25%) grade I, and 2/16 (12.5%) grade III. Cox-2 overexpression occurred in 3/16 (18.75%) samples, with positive correlation between Cox-2 expression and cellularity (r = 0.696, P = 0.003). Mitotic index lower than 9 events was found in 11/16 (68.7%) samples and between 10 and 19 mitotic events in 5/16 (31.3%) cases. Mean Ki-67 expression was 2.39 ± 2.48%. FISS characterized as fibrosarcomas presented longer overall survival (median 545 days) than other histological subtypes (median 130.5 days) [P = 0.01].Discussion: Patients with FISS generally present with larger nodules than those with sarcomas not associated to injections, suggesting a challenge for pet owners to note subcutaneous tumors in the interscapular region, in addition to the aggressive biological behavior of FISS. The influence of size on prognosis remains controversial. An association between histologic grade and the development of metastasis has been observed, with patients with grade III FISS associated with an increase in the metastatic rate. The present study did not find a correlation between overall survival and histologic grade. A positive correlation between the presence of giant multinucleated cells and tumor grade has been observed.Despite the absence of such correlation in the present study, possibly due to a small sample, a trend for higher frequency of giant cells in advanced histologic grade was observed. Cox-2 expression in 81.75% and overexpression in 18.75% of our samples contrasts with the 64% Cox-2 expression and the absence of Cox-2 expression found by other authors. A positive moderate correlation between cellularity and Cox-2 expression was also observed, while another study did not find a correlation of Cox-2 expression with tumor grade, recurrence rates or overall survival. Cox metabolites such as prostaglandins can enhance cellular proliferation, inhibit apoptosis, induce angiogenesis, alter cellular adherence to facilitate metastatic development and inhibit immune surveillance. In the present study, no correlation was found between Cox-2 and angiogenesis in FISS. Our findings demonstrated low immunolabeling for Ki-67. A previous study analyzed 52 samples of FISS, 51% of them considered grade III, with a mean Ki-67 labeling of 14%. The lower Ki-67 staining in the samples of the present study may be related to the lower number of samples of grade III FISS or to a difference in the studied population. Fibrosarcomas are associated with better prognosis than other histological subtypes. Furthermore, malignant peripheral nerve sheath tumors were diagnosed as a possible histological subtype of FISS.

scholarly journals Large Cell Acanthoma of the Conjunctiva: Clinicopathologic and Immunohistochemical Features

2019 ◽  
Vol 5 (5) ◽  
pp. 312-318
Author(s):  
Frederick A. Jakobiec ◽  
Paula Cortes Barrantes ◽  
Lina Ma ◽  
John Mandeville
Keyword(s):  
Large Cell ◽  
Accurate Diagnosis ◽  
Proliferation Index ◽  
Real Potential ◽  
Ki 67 ◽  
The Third ◽  
Exposed Skin ◽  
Eyelid Skin ◽  
Initial Surgery ◽  
Clinical Photograph

Large cell acanthoma (LCA) was first described as a lesion on sun-exposed skin. All LCAs feature keratinocytes twice the size of normal cells (cytomegaly). Although infrequently diagnosed in the skin, it has been even more rarely described by ophthalmic pathologists in the eyelid skin and the conjunctiva. This report describes the third case of a conjunctival epithelial LCA, with the first published clinical photograph highlighting its leukoplakic and well-circumscribed character, as well as the most thorough analysis of the immunohistochemical features of this lesion. It is contrasted with squamous dysplasias and papillomas of the conjunctiva. A review of previous conjunctival LCA lesions discloses frequent recurrences after initial surgery and the remote but real potential for squamous dysplastic transformation. Immunohistochemical stains for certain cytokeratins, p53, and Ki-67 (proliferation index) will in the future be particularly helpful in establishing an early and accurate diagnosis of conjunctival LCA.

Canine Neuroendocrine Tumors of the Pancreas: A Study Using Image Analysis Techniques for the Discrimination of Metastatic Versus Nonmetastatic Tumors

1997 ◽  
Vol 34 (2) ◽  
pp. 138-145 ◽  
Author(s):  
G. Minkus ◽  
U. Jütting ◽  
M. Aubele ◽  
K. Rodenacker ◽  
P. Gais ◽  
...  
Keyword(s):  
Image Analysis ◽  
Neuroendocrine Tumors ◽  
Nucleolar Organizer ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Nucleolar Organizer Regions ◽  
Ki 67 ◽  
Analysis Techniques ◽  
Significant Difference ◽  
Image Analysis Techniques

Canine pancreatic neuroendocrine tumors were studied using different image analysis techniques (nuclear image histometry, analysis of argyrophilic proteins of nucleolar organizer regions, determination of the mouse anti-Ki 67 antigen proliferation index, and DNA densitometry) to correlate their biological behavior with objective phenotypic markers. The methods were compared to determine the best method for distinguishing between metastatic and nonmetastatic tumors. Discrimination between the two types of tumor was possible using nuclear image histometry in combination with morphometric analysis of argyrophilic proteins of nucleolar organizer regions. In contrast, the mouse anti-Ki 67 antigen proliferation index, DNA measurement, and immunohistochemical parameters revealed no significant difference between the two types of tumors.

A retrospective study of immunohistochemical detection of Ki67 and correlation with ER, PR, and HER2-neu status in invasive breast cancer.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e21132-e21132
Author(s):  
Deepa Meda Prakash ◽  
Swarna Shivakumar ◽  
P P Bapsy
Keyword(s):  
Breast Cancer ◽  
Prognostic Factor ◽  
Tumor Size ◽  
Histological Grade ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Poor Correlation ◽  
Nuclear Staining ◽  
Ki 67 ◽  
Therapeutic Decisions

e21132 Background: Carcinoma breast is a heterogeneous group of disease. Several prognostic factors are known to predict the biological behavior and clinical outcome of breast cancer, including proliferation index, tumor size, age, histological grade, hormone receptors and Her2Neu status. Of theses proliferative index is the most important parameter in predicting tumor aggressiveness and prognosis. Ki67 is a nuclear protein, tightly linked to the cell cycle and is a marker of cell proliferation. High Ki67 usually correlates with a poor prognosis in breast cancer. Methods: This exploratory study is initiated whether Ki67 will be an additional prognostic factor. Immunohistochemical staining of Ki 67 was performed on formalin fixed paraffin embedded blocks of 50 cases of histologically confirmed breast cancer. Ki 67 expression based on distinct nuclear staining of tumor cells was assessed semiquantitatively as, nil (no immunostaining), low (≤10% immunopositivity), high (>10% immunopositivity) by light microscope. ER, PR, Her 2neu status and clinico-pathological parameters were analyzed and correlated with Ki67 immunostaining using the x2 test. Results: Our study showed high Ki 67 staining in 24 cases(48%), low immunostaining in 18 cases(36%) and was nil in 5 cases (10%) and was inconclusive in 3 cases(6%) (core biopsies). There was an inverse correlation of Ki 67 with ER, PR scores in 32 cases (64%) and positive correlation was seen in 28 cases (56%) with Her2 neu status. A good correlation was also observed with mitotic index in 37cases (74%) and histological grade in 29 cases (58%). Good correlation was seen with large tumor size in 24 cases(48%) and metastatic disease 9 cases (64%). Poor correlation was observed with node positive 22 cases (44%). High Ki 67 expression with good treatment response was seen in 13 cases (26%). Conclusions: Based on our study, Ki 67 score may prove to be an important prognostic factor in breast cancer and thereby, useful in making therapeutic decisions. But this is being explored in a large prospective study.

scholarly journals A Rare Case of Aggressive Systemic Mastocytosis Involving the Gastrointestinal System and Review of Literature

2021 ◽  
Author(s):  
Jianjun Wang ◽  
Zhong Zheng ◽  
Feng-nan Niu ◽  
Ting Wang ◽  
Biao Zhang ◽  
...  
Keyword(s):  
Mast Cells ◽  
Tumor Cells ◽  
Systemic Mastocytosis ◽  
Clinical Manifestations ◽  
Skin Lesions ◽  
Proliferation Index ◽  
Pelvic Cavity ◽  
Ki 67 ◽  
S 100 ◽  
Hepatic Portal

Abstract Background Systemic mastocytosis is a rare disease and most patients have pigmented urticarial skin lesions. It can be easily missed and misdiagnosed in small biopsies, especially in those patients with nonspecific clinical complaints or untypical skin lesions. Case presentation We report a case of 69-year-old man who have presented with 2-year of diarrhea, progressive weight loss of 20kg and abdominal distention for 3 months. Ultrasound and abdominal CT scan showed massive effusions in abdominal and pelvic cavity. Colonoscopy was performed and showed intensive mucosal proliferations forming polypoid appearances. Microscopically, monotonously uniform, small, round tumor cells with slightly rich cytoplasm were concentrated between the residual glands in the colonoscopic biopsy. The tumor cells showed positive expression of CD117 and S-100, and low Ki-67 proliferation index of 2%, while Trypsin, CK, CD68, CD1a, Langerin, Syn, CgA, CD56, SATB2, CD20, CD3, α-inhibin and SMA were all negative. KIT D816V mutation was detected as well. Liver biopsy showed that CD117 positive cells were more than 15 cells in aggregates around the hepatic portal area and less than 15% mast cells were found in bone marrow smears. No multiple or diffuse pattern of mast cells infiltration was seen by repeated skin biopsies of skin lesions. With all these considered, the diagnosis of aggressive systemic mastocytosis was made. Conclusions The diagnosis of aggressive systemic mastocytosis is challenging for untypical clinical manifestations and subtle or inconspicuous lesions, especially in endoscopic biopsies, which requires awareness and a close teamwork of pathologists and clinicians.

scholarly journals Value of Apparent Diffusion Coefficient for Assessing Preoperative T Staging of Low Rectal Cancer and Whether This Is Correlated With Ki-67 Expression

2020 ◽  
Vol 71 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Weiqun Ao ◽  
Xiangdong Bao ◽  
Guoqun Mao ◽  
Guangzhao Yang ◽  
Jian Wang ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Diffusion Coefficient ◽  
Apparent Diffusion Coefficient ◽  
Low Rectal Cancer ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Ki 67 ◽  
Adc Value ◽  
Apparent Diffusion ◽  
Adc Values

Purpose: To explore the value of the apparent diffusion coefficient (ADC) in assessing preoperative T staging of low rectal cancer and the correlation between ADC value and Ki-67 expression. Methods: Data on 77 patients with a proven pathology of low rectal cancer were retrospectively analyzed. All patients underwent a magnetic resonance imaging scan 1 week prior to operation, and the mean ADC value was measured. All tumors were fully removed, and pathologic staging was determined. The Ki-67 expression was determined using immunohistochemical methods in all patients. The correlation between Ki-67 expression and ADC features was studied. Results: A total of 77 patients with low rectal cancer were included in the study. The pathology type was adenocarcinoma. The numbers of patients with pathological stages T1, T2, T3, and T4 were 9, 23, 32, and 13, respectively. The ADC value of all tumors ranged from 0.60 to 1.20 mm2/s. The average Ki-67 proliferation index was 55.3% ± 20.2%. A significant difference was observed between the preoperative ADC value and pathological T staging of low rectal cancer ( P < .01). The more advanced the T stage, the lower the detected ADC values were. A negative correlation was noted between the preoperative ADC value and Ki-67 proliferation index of rectal cancer ( r = −0.71, P < .01). When the Ki-67 proliferation index increased, lower ADC values were detected. Conclusion: The ADC values can provide useful information on preoperative tumor staging and may facilitate evaluation of the biological behavior of low rectal cancer. The ADC values should be considered a sensitive image biomarker of rectal cancer.

Immunohistochemical Characterization and Evaluation of Prognostic Factors in Canine Oral Melanomas with Osteocartilaginous Differentiation

2007 ◽  
Vol 44 (5) ◽  
pp. 676-682 ◽  
Author(s):  
J. Sánchez ◽  
G. A. Ramirez ◽  
A. J. Buendia ◽  
M. Vilafranca ◽  
C. M. Martinez ◽  
...  
Keyword(s):  
Mitotic Index ◽  
Clinical Characteristics ◽  
Clinical Course ◽  
Immunohistochemical Analysis ◽  
Human Medicine ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Proliferation Marker ◽  
Prognosis Factors ◽  
Ki 67

Melanomas are the most common malignant oral neoplasm in dogs. Osteocartilaginous differentiation in oral melanomas is a rare feature described both in veterinary and human medicine. Here, 10 cases of this type of neoplasm were used to study their immunohistochemical, biological, and clinical characteristics. Reactivity for S100 and melan A antigen was evaluated, and 4 prognosis factors (mitotic index, invasiveness of epithelium, nuclear atypia, and proliferation index) were analyzed and correlated with the clinical course of the neoplasms after diagnosis. Immunohistochemical analysis of the studied neoplasms, including the osteocartilaginous areas, showed positive immunoreaction for S100 and melan A, except in one dog, which was negative for melan A. Analysis of the results showed that oral melamonas with osteocartilaginous differentiation have a clinical course similar to that of other melanomas in the oral cavity. Analysis of the mitotic index and the expression of proliferation marker Ki-67 could be useful tools for predicting the biological behavior of these neoplasms.

Relationship of intraoperative ultrasound characteristics with pathological grades and Ki-67 proliferation index in intracranial gliomas

2014 ◽  
Vol 42 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Ozdil Baskan ◽  
Gokalp Silav ◽  
Ramazan Sari ◽  
Ozlem Canoz ◽  
Ilhan Elmaci
Keyword(s):  
Intraoperative Ultrasound ◽  
Proliferation Index ◽  
Ki 67 ◽  
Relationship Of

scholarly journals Histopathological Features and Immunophenotype of Primary Gastric Invasive Fibromatosis

2020 ◽  
Author(s):  
Yangkun Wang ◽  
Zhishang Zhang ◽  
Bo Jiang ◽  
Chaoya Zhu ◽  
Sunan Wang ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Tumor Tissue ◽  
Gastric Wall ◽  
Clinical Manifestations ◽  
Nerve Tissue ◽  
Proliferation Index ◽  
Fatty Tissue ◽  
Ki 67 ◽  
Positive Expression ◽  
S 100

Abstract Background To investigate the histopathological characteristics, immunophenotype and differential diagnosis of primary gastric invasive fibromatosis.Methods The clinical manifestations, histological morphology and immunophenotype of 4 cases of primary gastric invasive fibromatosis were observed and related literatures were reviewed.Results Among the 4 patients, 2 were males and 2 were females, aged 28 and 47 years, respectively. The lesions were located in the stomach in 2 cases, the gastric antrum in 1 case and cardia in 1 case. Under the microscope, the tumor was located in the submucosa, growing infiltratingly, and infiltrating into the gastric wall muscularis, serous membrane and extraserous fatty tissue. Tumor cells were rich in cytoplasm, with unclear cell boundaries, long rod-shaped nuclei, deep chromatin, no atypia, and rare mitotic figures. The tumor tissue was composed of proliferating spindle-shaped fibroblasts and collagen fibers, and the cell morphology was relatively uniform, arranged in parallel bundles or staggered weaves. Tumor tissue invaded and destroyed smooth muscle, blood vessels, nerve tissue and adipose tissue of the gastric wall. Immunophenotype: positive expression of vimentin, β-catenin, SMA positive expression; CKpan, EMA, S-100 protein, desmin, CD99, Bcl-2, ALK, CD34, CD117, DOG1, hormone receptors were all negative; The cell proliferation index ki-67 positive cells were 3–5%.Conclusion Primary invasive fibromatosis of the stomach is a relatively rare spindle cell tumor, which needs to be differentiated from tumors and pathological lesions such as inflammatory myofibroblastic tumor, plexiform mucinous fibroma, gastrointestinal stromal tumor, etc.

scholarly journals HAEMANGIOPERICYTOMA

2015 ◽  
Vol 22 (03) ◽  
pp. 312-316
Author(s):  
Xiang Longquan ◽  
Henry Mwakyoma
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tumor Cells ◽  
Clinical Manifestations ◽  
Proliferation Index ◽  
High Recurrence Rate ◽  
Imaging Features ◽  
Rare Tumor ◽  
Ki 67 ◽  
Fibrous Tumor

Hemangiopericytoma (HPC) in central nervous system is a rare tumor, his tumorhas a high recurrence rate and the characteristics of extracranial metastases. Objectives:To investigate the clinicopathological features, imaging features, immunohistochemicalphenotype of haemangiopericytoma (HPC) of central nervous system. Design: Hospital basedcrossectional prospective study. Period: From 24th October to 26th October 2012. Setting: FirstPeople’s Hospital of Jining City, China. Methods: The clinical manifestations, imaging features,histopathological and immunohistochemical features were analyzed combining the review of theliterature in one case of central HPC. Results: The Gross examination revealed the size of thetumor was 5cm × 4cm× 1.5cm; the section is gray, medium soft texture, and part of the area hadcapsule. The microscopic examination showed that the tumor cells were abundant and the samesize, showing round, oval or short spindle shape. The cytoplasm was eosinophilic, and part ofit was slightly translucent. The nuclei were ovoid, and the nucleoli were inconspicuous.A lot ofcapillaries lined by endothelial cells were seen in the tumor tissue, and the blood vessels weredilated like “staghorn” in some areas. Immunohistochemistry showed that tumor cells expressedVimentin, CD34, CD99, Bcl-2, PR protein. They didn’t express EMA, SMA, and S-100 protein.The proliferation index of ki-67 is about 4%. Conclusions: The central haemangiopericytomais a rare tumor, having no specific clinical manifestations and imaging features. The finaldiagnosis requires a combination of histopathological and immunohistochemical examination,and it should be differentiated from meningioma, solitary fibrous tumor, hemangioblastoma andmesenchymal chondrosarcoma, etc.

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