scholarly journals Similarity Assessment by Multivariate Statistics Method Based on Distance Between Biosimilar and Originator

2020 ◽  
Author(s):  
Jian Xu ◽  
Zhihui Shao ◽  
Xiaoxiong Han ◽  
Yingfeng Huang ◽  
Xun Zou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Data Analysis ◽  
Design Of Experiments ◽  
Multivariate Data Analysis ◽  
Culture Conditions ◽  
Analysis Method ◽  
Glycosylation Pattern ◽  
Pattern Similarity ◽  
And Function ◽  
Specific Distance

Abstract The development of biosimi lar products or follow on biologics has been flourishing in recent years because of lower price than originators. In th is study, a multivariate data analysis method based on JMP software was proposed to assess the glycosylation pattern similarity of antibo dy candidates from different conditions in optimization experiment with reference. A specific distance was generated by t specific distance was generated by this method and indicated the glycoform similarity his method and indicated the glycoform similarity between biosimilar and reference. This method can be applied to analyze the similaritbetween biosimilar and reference. This method can be applied to analyze the similarity of y of other physicochemical and function characters between followother physicochemical and function characters between follow--on biologics and originators. on biologics and originators. Then the design of experiThen the design of experiments method can be realized to optimize culture conditions of cellments method can be realized to optimize culture conditions of cell culture to attain similar antibody candidatesculture to attain similar antibody candidates..

scholarly journals Similarity assessment by multivariate statistics method based on the distance between biosimilar and originator

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jian Xu ◽  
Zhihui Shao ◽  
Xiaoxiong Han ◽  
Yingfeng Huang ◽  
Xun Zou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Data Analysis ◽  
Multivariate Statistics ◽  
Multivariate Data Analysis ◽  
Experimental Methods ◽  
Analysis Method ◽  
Glycosylation Pattern ◽  
Pattern Similarity ◽  
Basal Media ◽  
Specific Distance

AbstractThe development of biosimilar products or follow-on biologics has been flourishing in recent years because of their lower price than the originators. In this study, a multivariate data analysis method based on JMP software was proposed to assess the glycosylation pattern similarity of antibody candidates from different conditions in optimization experiments with a reference. A specific distance was generated by this method and indicated the glycoform similarity between the biosimilar and the reference. This method can be applied to analyze the similarity of other physicochemical and functional characteristics between follow-on biologics and originators. Then, the design of experimental methods can be realized to optimize the conditions of cell culture to attain similar antibody candidates. A higher concentration of GlcNAc added to the basal media made the glycan of the antibody more similar to the glycan of the reference in this study.

scholarly journals Vessel-on-a-chip models for studying microvascular physiology, transport, and function in vitro

2020 ◽  
Author(s):  
Savannah R. Moses ◽  
Jonathan J. Adorno ◽  
Andre F. Palmer ◽  
Jonathan W. Song
Keyword(s):  
Cell Culture ◽  
Tissue Level ◽  
Culture Conditions ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Microscale Flow ◽  
Flow Phenomena ◽  
Defined Culture ◽  
And Function

To understand how the microvasculature grows and remodels, researchers require reproducible systems that emulate the function of living tissue. Innovative contributions toward fulfilling this important need have been made by engineered microvessels assembled in vitro using microfabrication techniques. Microfabricated vessels, commonly referred to as "vessels on a chip," are from a class of cell culture technologies that uniquely integrate microscale flow phenomena, tissue-level biomolecular transport, cell-cell interactions, and proper 3-D extracellular matrix environments under well-defined culture conditions. Here, we discuss the enabling attributes of microfabricated vessels that make these models more physiological compared to established cell culture techniques, and the potential of these models for advancing microvascular research. This review highlights the key features of microvascular transport and physiology, critically discusses the strengths and limitations of different microfabrication strategies for studying the microvasculature, and provides a perspective on current challenges and future opportunities for vessel on a chip models.

scholarly journals The Realizations and Functions of Figurative Speeches in Novels Written by Prasetyo Utomo

2020 ◽  
Vol 9 (2) ◽  
pp. 184-191
Author(s):  
Fajar Arifiyanto ◽  
Agus Nuryatin ◽  
Mukh Doyin
Keyword(s):  
Data Analysis ◽  
Data Collection ◽  
Figurative Language ◽  
Analysis Method ◽  
Aesthetic Value ◽  
Form And Function ◽  
Language Style ◽  
Language Styles ◽  
Almost All ◽  
And Function

Utomo’s novels are very related to the theme of religion and culture. The language used tends to be simple but still interesting and beautiful so research on the form and function of language style is very interesting. The purpose of this study is to describe the form and function of language style in the three Utomo novels. Data collection is done by heuristic techniques or read by repeatedly. Data analysis method used is hermeneutics. Almost all types of figurative language styles are found, but for the sake of effective exposure, only three data would be presented as examples of the form of language style and the function of language style. The three most found figurative languages are metaphor, simile, hyperbole, personification, and sinekdoke. The function of language also varies, starting from clarifying the picture, increasing the impression, shortening the narrative, and describing the character's feelings. Fulfillment of these functions with simple but beautiful forms of language style makes Utomo's novels have high aesthetic value and are easily understood by readers.

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