scholarly journals SFRP2 Promotes Peritoneal Metastasis of Ovarian Cancer within Phosphorylation of GSK3β

2021 ◽  
Author(s):  
Tengfei Zhang ◽  
Shimin Chen Qin ◽  
Jianrong Yuan ◽  
Jianlong Zhao ◽  
Yutong Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Peritoneal Metastasis ◽  
Biological Effects ◽  
High Expression ◽  
Ovarian Cancer Cells ◽  
Sequencing Data ◽  
Data Set ◽  
Invasion And Migration ◽  
And Migration

Abstract BackgroundThe aim was to study further the molecular mechanism of ovarian cancer peritoneal metastasis and the corresponding prognostic markers.MaterialsWe analysed the sequencing data of 10 pairs of primary ovarian cancer and peritoneal metastasis from GSE98281 to determine SFRP2 through GO, KEGG and GSEA analysis. Western blot, invasion and migration experiments were used to detect the biological effects of SFRP2 on ovarian cancer cells. Immunohistochemistry was used to detect ovarian cancer in tissue samples from a TCGA-Ovarian Cancer cohort, and the survival analysis of SFRP2 was then performed.ResultsSFRP2 affected the phosphorylation level of GSK3β and upregulated the expression level of β-catenin. The high expression of SFRP2 in ovarian cancer cells improves cell invasion and migration capabilities. In histology studies, high expression of SFRP2 has increased the positive expression of β-catenin in the nucleus, and patients with high expression of SFRP2 had a worse prognosis. Similar results also appeared in ovarian cancer cases with high expression of SFRP2 mRNA in the TCGA data set.ConclusionSFRP2 activates the β-Catenin/Wnt signalling pathway through phosphorylation of GSK3β, which promotes the metastasis of ovarian cancer that leads to poor prognosis.

scholarly journals Long non-coding RNA XIST regulates ovarian cancer progression via modulating miR-335/BCL2L2 axis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Qingjuan Meng ◽  
Ningning Wang ◽  
Guanglan Duan
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Invasion And Migration ◽  
Non Coding Rna ◽  
And Migration ◽  
Long Non Coding Rna

Abstract Background X inactivation-specific transcript (XIST) is the long non-coding RNA (lncRNA) related to cancer, which is involved in the development and progression of various types of tumor. However, up to now, the exact role and molecular mechanism of XIST in the progression of ovarian cancer are not clear. We studied the function of XIST in ovarian cancer cells and clinical tumor specimens. Methods RT-qPCR was performed to detect the expression levels of miR-335 and BCL2L2 in ovarian cancer cells and tissues. MTT and transwell assays were carried out to detect cell proliferation, migration, and invasion abilities. Western blot was performed to analyze the expression level of BCL2L2. The interaction between miR-335 and XIST/BCL2L2 was confirmed using a luciferase reporter assay. Results The inhibition of XIST can inhibit the proliferation invasion and migration of human ovarian cancer cells. In addition, the miR-335/BCL2L2 axis was involved in the functions of XIST in ovarian cancer cells. These results suggested that XIST could regulate tumor proliferation and invasion and migration via modulating miR-335/BCL2L2. Conclusion XIST might be a carcinogenic lncRNA in ovarian cancer by regulating miR-335, and it can serve as a therapeutic target in human ovarian cancer.

scholarly journals MiRNA-802 suppresses proliferation and migration of epithelial ovarian cancer cells by targeting YWHAZ

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Bo Yang ◽  
Li Sun ◽  
Lei Liang
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Target Genes ◽  
Gene Prediction ◽  
Biological Effects ◽  
Expression Level ◽  
Ovarian Cancer Cells ◽  
And Migration

Abstract Background The imbalance of expression of microRNA-802 may have a significant place in tumor progression. However, the bio-function of epithelial ovarian cancer cells remains unclear. Therefore, we setup this study to explore the pathogenesis of epithelial ovarian cancer based on microRNA-802. Methods RT-qPCR analysis was used to measure the expression level of microRNA802 and YWHAZ in epithelial ovarian cancer. CCK-8, colony formation, flow cytometry and transwell assay were used to detect the effects of microRNA-802 on cell proliferation, apoptosis, invasion and migration. Target gene prediction and screening, luciferase reporting experiments were applied to validate the downstream target genes of microRNA-802. The effects of microRNA-802 on the expression of YWHAZ and its biological effects were measured by Western blotting and RT-qPCR. Results Compared with normal cell lines and tissues, the expression level of microRNA-802 was obviously down-regulated in cancer related cell lines and tissues. Overexpression of microRNA-802 could obviously inhibit the invasion and proliferation and induce apoptosis. In addition, YWHAZ was the binding target protein of miR-802 for epithelial ovarian cancer cells. YWHAZ was obviously up-regulated in human epithelial ovarian cancer cells, and YWHAZ was negatively correlated with the expression of miR-802. YWHAZ can partly eliminate the inhibitory effect caused by overexpression of miR-802 on growth and metastasis of epithelial ovarian cancer cells. Conclusion miR-802 can regulate the occurrence and development of epithelial ovarian cancer by targeting YWHAZ.

scholarly journals circ-CSPP1 promotes proliferation, invasion and migration of ovarian cancer cells by acting as a miR-1236-3p sponge

2019 ◽  
Vol 114 ◽  
pp. 108832 ◽  
Author(s):  
Qian-hui Li ◽  
Yao Liu ◽  
Shuo Chen ◽  
Zhi-hong Zong ◽  
Yu-ping Du ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Invasion And Migration ◽  
And Migration

scholarly journals Functional implications of PABPC1 in the development of ovarian cancer

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 805-815
Author(s):  
Cong Feng ◽  
Yan-Hua Han ◽  
Na Qi ◽  
Jia Li ◽  
Qing-Hua Sheng ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Mesenchymal Transition ◽  
Underlying Mechanism ◽  
Gene Expression Omnibus ◽  
The Cancer Genome Atlas ◽  
Sequencing Data ◽  
Data Set ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
And Migration

Abstract This research aimed to probe the expression characteristics of poly(A)-binding protein cytoplasmic 1 (PABPC1) and its role on the phenotype of ovarian cancer (OC) cells and to further investigate the possible underlying mechanism. The expression of PABPC1 was analyzed according to the data from gene expression omnibus, The Cancer Genome Atlas (TCGA) and Oncomine databases and the RNA sequencing data set from TCGA were downloaded for evaluating the prognostic values. We revealed that compared with the healthy samples, PABPC1 was upregulated in OC samples. High expression of PABPC1 had a connection with a shorter survival for patients with OC. Loss and gain of function assays revealed that silencing PABPC1 significantly suppressed the viability, invasion and migration of SK-OV-3 cells, while PABPC1 overexpression in A2780 cells showed the reverse outcomes. Moreover, Western blot demonstrated that silencing PABPC1 notably inactivated the epithelial–mesenchymal transition (EMT) process, while upregulation of PABPC1 promoted the mitigation of epithelial phenotype and the acquisition of mesenchymal phenotype. Taken together, PABPC1 was upregulated in OC cells and served as a carcinogene to promote the OC cell growth and invasion partly by modulating the EMT process, which implied that PABPC1 might be considered as a useful biomarker for OC therapeutics.

LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN

2018 ◽  
Vol 67 (11-12) ◽  
pp. 927-936 ◽  
Author(s):  
Juelan Wang ◽  
Wenqian Xu ◽  
Yangke He ◽  
Qi Xia ◽  
Siwei Liu
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Invasion And Migration ◽  
And Migration

scholarly journals High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Vanessa A. Enriquez ◽  
Ellane R. Cleys ◽  
Juliano C. Da Silveira ◽  
Monique A. Spillman ◽  
Quinton A. Winger ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Cells ◽  
Rna Binding ◽  
Tumor Development ◽  
Hek293 Cells ◽  
Ovarian Cancer Cells ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
And Migration

Epithelial ovarian cancer is the most aggressive and deadly form of ovarian cancer and is the most lethal gynecological malignancy worldwide; therefore, efforts to elucidate the molecular factors that lead to epithelial ovarian cancer are essential to better understand this disease. Recent studies reveal that tumor cells release cell-secreted vesicles called exosomes and these exosomes can transfer RNAs and miRNAs to distant sites, leading to cell transformation and tumor development. The RNA-binding protein LIN28 is a known marker of stem cells and when expressed in cancer, it is associated with poor tumor outcome. We hypothesized that high LIN28 expressing ovarian cancer cells secrete exosomes that can be taken up by nontumor cells and cause changes in gene expression and cell behavior associated with tumor development. IGROV1 cells were found to contain high LIN28A and secrete exosomes that were taken up by HEK293 cells. Moreover, exposure to these IGROV1 secreted exosomes led to significant increases in genes involved in Epithelial-to-Mesenchymal Transition (EMT), induced HEK293 cell invasion and migration. These changes were not observed with exosomes secreted by OV420 cells, which contain no detectable amounts of LIN28A or LIN28B. No evidence was found of LIN28A transfer from IGROV1 exosomes to HEK293 cells.

Sign in / Sign up

Export Citation Format

Share Document