scholarly journals Simultaneous Measurement of Tadehaginoside and its Principal Metabolite in Rats by HPLC–MS/MS and its Application in Pharmacokinetics and Tissue Distribution Study

2021 ◽  
Author(s):  
Cai-Yun Zhang ◽  
Ya-Ting Lu ◽  
Yin-Feng Tan ◽  
Lin Dong ◽  
Zhi-Heng Su ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
High Performance ◽  
Biological Activities ◽  
Intragastric Administration ◽  
Distribution Study ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Tissue Distribution Study

Abstract Background Tadehaginoside, an active ingredient isolated from Tadehagi triquetrum L., exhibited various biological activities. However, the pharmacokinetics and tissue-distribution which affects tadehaginoside’s therapeutic actions and application remain elusive.MethodsTo clarify the metabolism of tadehaginoside in vivo, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to detect the level of tadehaginoside in plasma and eleven tat tissues (brain, heart, liver, spleen, lungs, kidneys, stomach, small intestine, skeletal muscle, body fat, and testes). Besides, this validated method was also successfully applied to the quantitative determination of its metabolite, p-hydroxycinnamic acid (HYD) in plasma. The pharmacokinetic and tissue-distribution of tadehaginoside were investigated by this developed method. ResultsThe pharmacokinetic study indicated that tadehaginoside in plasma of rats with intragastric administration showed relatively low concentration may be due to the formation of its metabolite, and the quick absorption of tadehaginoside was detected following intravenous administration. Tissue-distribution study indicated that kidney and spleen were the major distribution organs for tadehaginoside in rats. ConclusionsThese results could provide clues for exploring the bioactivity of tadehaginoside based on its pharmacokinetic characteristics.

Determination of bosutinib in mice plasma and tissue by UPLC-MS/MS and its application to the pharmacokinetic and tissue distribution study

2015 ◽  
Vol 7 (21) ◽  
pp. 9184-9189 ◽  
Author(s):  
Linxia Wang ◽  
Luming Tang ◽  
Yi Zheng ◽  
Guoquan Pan ◽  
Wei Zhu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Internal Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Distribution Study ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Tissue Distribution Study

A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine bosutinib in mice plasma and tissue using diazepam as the internal standard (IS).

scholarly journals A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in Croton Crassifolius

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 694
Author(s):  
Jin-Long Tian ◽  
Chi Shu ◽  
Ye Zhang ◽  
Hui-Jun Cui ◽  
Xu Xie ◽  
...  
Keyword(s):  
High Performance ◽  
Rapid Determination ◽  
Biological Activities ◽  
Bioactive Constituents ◽  
Complex Species ◽  
Chromatography Tandem Mass Spectrometry ◽  
Homogenate Extraction ◽  
Liquid Chromatography Tandem Mass ◽  
Clerodane Diterpenoids

Clerodane diterpenoids are the main bioactive constituents of Croton crassifolius and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS) technique together for the rapid extraction and determination of clerodane diterpenoids in C. crassifolius. All calibration curves showed good linearity (r > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE–UHPLC–MS method only took 5 min to extract nine clerodane diterpenoids in C. crassifolius and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC–MS was a feasible method for QC of clerodane diterpenoids in C. crassifolius, and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.

scholarly journals Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MSALL-Based Approach

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5644
Author(s):  
Yixuan Feng ◽  
Lele Li ◽  
Yuxuan Li ◽  
Xinxin Zhou ◽  
Xiaoying Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Tissue Distribution ◽  
High Performance ◽  
Time Of Flight ◽  
Stomach Muscle ◽  
Distribution Study ◽  
Flight Mass Spectrometry ◽  
Tissue Distribution Study

Poloxamer188 (PL188), as one of the most commonly used pharmaceutical excipients, has unique physicochemical properties and good biocompatibility, and so is playing an increasingly extensive role in the field of medicine. Currently, there are few studies on the tissue distribution of PL188 in vivo. In this study, the LC-MS method based on MSALL technique of quadrupole time of flight mass spectrometry for absolute quantitative analysis of poloxamer 188 in biological substrates was established for the first time. The tissue distribution of poloxamer188 in SD rats were studied using the established quantitative analysis method. To explore the distribution of PL188 in organs and tissues, PL188 was administered via rat tail vein at a dose of 5 mg/kg. Eight kinds of tissues including heart, liver, spleen, lung, kidney, stomach, muscle and brain of rats were collected at 0.25 h, 1 h and 4 h after administration. Tissue distributions showed the highest level was observed in kidney, then in stomach, which indicated PL188 mainly bioaccumulated in the kidney. This study can provide references for the further study of PL188.

A sensitive UPLC-APCI-MS/MS method for the determination of dexamethasone and its application in an ocular tissue distribution study in rabbits following topical administration

2018 ◽  
Vol 10 (20) ◽  
pp. 2307-2316 ◽  
Author(s):  
Murali K. Matta ◽  
Suresh Narayanasamy ◽  
Christopher D. Thomas ◽  
Lin Xu ◽  
Sharron Stewart ◽  
...  
Keyword(s):  
Topical Administration ◽  
Ultra Performance Liquid Chromatography ◽  
Ocular Tissue ◽  
Spectrometry Method ◽  
Ocular Tissues ◽  
Distribution Study ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Tissue Distribution Study

A sensitive, specific and rapid ultra-performance liquid chromatography tandem mass spectrometry method has been developed and validated for the determination of dexamethasone (DEX) in ocular tissues of rabbits.

scholarly journals Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yan Huo ◽  
Yu-Qiang Liu ◽  
Zhong-Xu Bai ◽  
Qian Cai
Keyword(s):  
Tissue Distribution ◽  
Mobile Phase ◽  
High Performance ◽  
Rat Plasma ◽  
Processing Method ◽  
Distribution Study ◽  
Accuracy And Precision ◽  
Digestive Disorders ◽  
Tissue Distribution Study

In ChinaAtractylodis Rhizomais widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties ofAtractylodis Rhizoma, an investigation was carried out to compare the pharmacokinetics and tissue distribution of typical constituent after oral administration of rawAtractylodis Rhizomaand processed ones. A simple, rapid, and sensitive high performance liquid chromatography with UV detection was developed and validated for the determination of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma. A chromatography was carried out on Diamonsil C18(250 × 4.6 mm; 5 μm) analytical column, using a mobile phase which consisted of acetonitrile and 0.1% phosphoric acid water (60 : 40, v/v) at a flow rate of 1.0 mL·min−1. The wavelength was set at 336 nm. The LLOQ of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate was 0.00143 μg·mL−1. Both accuracy and precision were satisfactory. The pharmacokinetic results showed that theTmax⁡was 1 hour in advance and theCmax⁡was increased after processing. Tissue distribution showed that the highest level was in spleen. And the concentrations in the spleen were increased after stir-frying with bran.

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