scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Cigarette Smoke Condensate ◽  
Human Seminal Plasma ◽  
High Level ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 hours, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 hours, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.

scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Cigarette Smoke Condensate ◽  
Human Seminal Plasma ◽  
High Level ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level) were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.

scholarly journals Cigarette smoking is associated with abnormal ferroptosis in human sperm

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Human Sperm ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Human Semen ◽  
Cigarette Smoke Condensate ◽  
Progressive Motility ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of ferroptosis glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level) were observed in human semen and in GC-2 Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with abnormalities of ferroptosis in human sperm.

scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Semen Quality ◽  
High Level

Alpaca semen quality in relation to different diets

2013 ◽  
Vol 25 (4) ◽  
pp. 683 ◽  
Author(s):  
N. S. Juyena ◽  
J. Vencato ◽  
G. Pasini ◽  
I. Vazzana ◽  
C. Stelletta
Keyword(s):  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Seminal Plasma ◽  
Total Protein ◽  
Semen Quality ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Volume Viscosity ◽  
Glutamyl Transferase

The aim of the present study was to evaluate the biochemical composition of seminal plasma, along with semen quality, of alpacas maintained on different diets (hay; hay + pasture grazing; pasture grazing + sheep concentrate; pasture grazing + horse concentrate; Periods 1–4, respectively). Alpacas (n = 5) were fed the four different diets for a period of 6 weeks each. During the period of feeding of each diet, semen was collected using an artificial vagina to determine its volume, viscosity, sperm concentration and sperm motility. Moreover, testicular volume and body condition score were evaluated. Seminal plasma was analysed biochemically to measure total protein, triglyceride, cholesterol, γ-glutamyl transferase, alanine aminotransferase (ALT) and alkaline phosphatase levels. Protein profiles were investigated using one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis. There was high variability in semen parameters between different males maintained on the same diet. Semen volume increased significantly (P < 0.05) when alpacas were fed diets containing commercial sheep and horse concentrates. In contrast, sperm concentration and motility decreased significantly (P < 0.05) from Period 1 to Period 4. Dietary changes had no effect on viscosity. Significant reductions were seen in triglyceride and cholesterol content, as well as γ-glutamyl transferase, ALT and alkaline phosphatase concentrations, from Period 1 to Period 4. Regardless of experimental period, a wide variation was seen in seminal plasma enzyme concentrations between alpacas, whereas diet had no effect on glucose and total protein concentrations in the seminal plasma. Eight protein bands, with molecular weights ranging from 200 to 14 kDa, were considered in electrophoresis gel after image analysis. Proteins fractions of the 14-kDa (total protein express in md dL–1 with a molecular weight of 14-kDa, TP8) and 21-kDa (total protein express in md dL–1 with a molecular weight of 21-kDa, TP7) bands were not present in all samples of alpaca seminal plasma. There were no significant changes in the concentration of any protein fractions during the four periods. Moreover, the protein fraction of the 60-kDa (total protein express in md dL–1 with a molecular weight of 60-kDa, TP3) band was the most prevalent in all periods. These results demonstrate that there are marked changes in semen quality, as well as some parameters related to the composition of alpaca seminal plasma, that are dependent on diet, which may indicate the need for specific diet formulation to improve reproductive performance. We hypothesise that, in alpacas, the mechanisms underlying the changes in some reproductive traits in response to feeding regimens could be related to changes in the endocrine–gonadal system.

High level of ?1-acid glycoprotein in human seminal plasma is associated with high branching and expression of Lewisa groups on its glycans: Supporting evidence for a prostatic origin

The Prostate ◽  
2002 ◽  
Vol 52 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Dennis C.W. Poland ◽  
Ewa Kratz ◽  
Jan P.W. Vermeiden ◽  
Steffen M. De Groot ◽  
Ben Bruyneel ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Supporting Evidence ◽  
Human Seminal Plasma ◽  
Acid Glycoprotein ◽  
High Level

Characterization of Nuclease Activity in Human Seminal Plasma and its Relationship to Semen Parameters, Sperm DNA Fragmentation and Male Infertility

2016 ◽  
Vol 195 (1) ◽  
pp. 213-219 ◽  
Author(s):  
Alba Fernandez-Encinas ◽  
Agustí García-Peiró ◽  
Jordi Ribas-Maynou ◽  
Carlos Abad ◽  
María José Amengual ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Nuclease Activity ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Human Seminal Plasma ◽  
Sperm Dna

scholarly journals Decreased piRNAs in Infertile Semen Are Related to Downregulation of Sperm MitoPLD Expression

2021 ◽  
Vol 12 ◽  
Author(s):  
Yeting Hong ◽  
Yanqian Wu ◽  
Jianbin Zhang ◽  
Chong Yu ◽  
Lu Shen ◽  
...  
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Molecular Biomarkers ◽  
Loss Of Function ◽  
Human Seminal Plasma ◽  
High Concentrations ◽  
Tight Correlation ◽  
Infertile Patients

Currently, the molecular mechanisms underlining male infertility are still poorly understood. Our previous study has demonstrated that PIWI-interacting RNAs (piRNAs) are downregulated in seminal plasma of infertile patients and can serve as molecular biomarkers for male infertility. However, the source and mechanism for the dysregulation of piRNAs remain obscure. In this study, we found that exosomes are present in high concentrations in human seminal plasma and confirmed that piRNAs are predominantly present in the exosomal fraction of seminal plasma. Moreover, we showed that piRNAs were significantly decreased in exosomes of asthenozoospermia patients compared with normozoospermic men. By systematically screening piRNA profiles in sperms of normozoospermic men and asthenozoospermia patients, we found that piRNAs were parallelly reduced during infertility. At last, we investigated the expression of some proteins that are essential for piRNAs biogenesis in sperms and therefore identified a tight correlation between the levels of spermatozoa piRNA and MitoPLD protein, suggesting that the loss-of-function of MitoPLD could cause a severe defect of piRNA accumulation in sperms. In summary, this study identified a parallel reduction of piRNAs and MitoPLD protein in sperms of asthenozoospermia patients, which may provide pathophysiological clues about sperm motility.

scholarly journals Reactive oxygen species-induced alterations in H19-Igf2 methylation patterns, seminal plasma metabolites, and semen quality

2018 ◽  
Vol 36 (2) ◽  
pp. 241-253 ◽  
Author(s):  
Mahsa Darbandi ◽  
Sara Darbandi ◽  
Ashok Agarwal ◽  
Saradha Baskaran ◽  
Sulagna Dutta ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Reactive Oxygen ◽  
Plasma Metabolites ◽  
Oxygen Species ◽  
Methylation Patterns

scholarly journals Effect of Ivermectin on semen parameters and levels of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) enzymes in seminal plasma of Iraqi Awassi rams

2014 ◽  
Vol 13 (1) ◽  
pp. 115
Author(s):  
Ali. J. Ali
Keyword(s):  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Semen Quality ◽  
Harmful Effect ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Control Group ◽  
Volume Percentage ◽  
Semen Collection

The objective of this study was to evaluate the effect of Ivermectin on semen quality and estimation of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in seminal plasma. Six mature Awassi rams aged 2-3 years and weighing 38-42 Kg were used in this study. Semen collection was done weekly for five weeks from the same ram. The first two collection considered as training for the animal and discarded, the second two collection were considers for the control group. After administration of the drug (Ivermectin), semen samples were collected from the Awassi ram 1, 7,14 days. The parameter studies were included semen volume, percentage motility of sperm, sperm concentration, live sperm, and morphology percentage. Samples of seminal plasma were analyzed for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results of this study indicate that Ivermectin leads to significant (P<0.05) decreased in semen parameters after the first semen collection. Reaction time significantly higher (P<0.05) in the treated group after 1 day of injection. Alanine aminotransferase ALT, Aspartate aminotransferase AST show no significant differences in activities in seminal plasma during the study. In conclusion: it is preferable to use the animals for the purpose of reproduction only after at least 7 days after Ivermectin injection due to it harmful effect on semen quality.

scholarly journals Correlations between Different Heavy Metals in Diverse Body Fluids: Studies of Human Semen Quality

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Lidia Mínguez-Alarcón ◽  
Jaime Mendiola ◽  
Manuela Roca ◽  
José J. López-Espín ◽  
José J. Guillén ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Biological Fluids ◽  
Body Fluids ◽  
Male Reproduction ◽  
World Health ◽  
Semen Parameters ◽  
Human Semen ◽  
Health Organization

It has been hypothesized that exposure to heavy metals may impair male reproduction. To measure the effect produced by low doses of heavy metals on semen parameters, it is necessary to clarify in which body fluids those measurements must be performed. Sixty-one men attending infertility clinics participated in our study. Concentrations of lead, cadmium, and mercury were measured in whole blood, blood plasma, and seminal plasma using spectroanalytical and electrochemical methods. Semen analyses were performed according to World Health Organization criteria. For statistical analysis, Spearman's rank correlations, mean comparison tests, and discriminant analysis were calculated. Significant correlations between the measured concentrations of the three heavy metals in the same biological fluids were observed. However, no similar relationship was seen when comparing the concentrations in different body fluids of the same metal. According to our results and previous publications, seminal plasma might be the best body fluid for assessing impairment of human semen parameters.

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