scholarly journals Decreased piRNAs in Infertile Semen Are Related to Downregulation of Sperm MitoPLD Expression

2021 ◽  
Vol 12 ◽  
Author(s):  
Yeting Hong ◽  
Yanqian Wu ◽  
Jianbin Zhang ◽  
Chong Yu ◽  
Lu Shen ◽  
...  
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Molecular Biomarkers ◽  
Loss Of Function ◽  
Human Seminal Plasma ◽  
High Concentrations ◽  
Tight Correlation ◽  
Infertile Patients

Currently, the molecular mechanisms underlining male infertility are still poorly understood. Our previous study has demonstrated that PIWI-interacting RNAs (piRNAs) are downregulated in seminal plasma of infertile patients and can serve as molecular biomarkers for male infertility. However, the source and mechanism for the dysregulation of piRNAs remain obscure. In this study, we found that exosomes are present in high concentrations in human seminal plasma and confirmed that piRNAs are predominantly present in the exosomal fraction of seminal plasma. Moreover, we showed that piRNAs were significantly decreased in exosomes of asthenozoospermia patients compared with normozoospermic men. By systematically screening piRNA profiles in sperms of normozoospermic men and asthenozoospermia patients, we found that piRNAs were parallelly reduced during infertility. At last, we investigated the expression of some proteins that are essential for piRNAs biogenesis in sperms and therefore identified a tight correlation between the levels of spermatozoa piRNA and MitoPLD protein, suggesting that the loss-of-function of MitoPLD could cause a severe defect of piRNA accumulation in sperms. In summary, this study identified a parallel reduction of piRNAs and MitoPLD protein in sperms of asthenozoospermia patients, which may provide pathophysiological clues about sperm motility.

Measurement of transferrin in human seminal plasma by a chemiluminescent method.

1986 ◽  
Vol 32 (1) ◽  
pp. 153-156 ◽  
Author(s):  
A L Caldini ◽  
C Orlando ◽  
T Barni ◽  
G Messeri ◽  
M Pazzagli ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dicarboxylic Acid ◽  
Seminal Plasma ◽  
Cell Function ◽  
Acid Hydrazide ◽  
Immunological Reaction ◽  
Human Seminal Plasma ◽  
One Year ◽  
Chemiluminescent Method ◽  
Infertile Patients

Abstract We describe a luminescence immunoassay for measuring transferrin in human seminal plasma. Human transferrin conjugated with 7-[(N-4-aminobutyl)-N-ethylamino]naphthalene-1,2-dicarboxylic acid hydrazide was used to monitor the immunological reaction. The conjugate was stable for at least one year. The sensitivity (2 ng per tube) of the assay allows measurement of this protein in diluted seminal plasma. Results by this method (y) correlated well (r = .9681) with those by a conventional RIA method (x): y = 1.000x + 1.646 micrograms per ejaculate. Seminal transferrin concentrations are reported for normal control subjects, vasectomized subjects, and infertile patients. The method described appears suitable for measurement of seminal transferrin as an index of Sertoli cell function in male infertility.

Simple, rapid determination of zinc and acid phosphatase in seminal plasma with an ABA-100 bichromatic analyzer.

1988 ◽  
Vol 34 (8) ◽  
pp. 1605-1607 ◽  
Author(s):  
M Gavella
Keyword(s):  
Acid Phosphatase ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Rapid Determination ◽  
Assay System ◽  
Human Seminal Plasma ◽  
Automated Assay ◽  
Sensitivity Specificity

Abstract I describe an automated assay for zinc and acid phosphatase in seminal plasma. These, which are markers of the function of the prostate, were assayed bichromatically with an Abbott ABA-100 analyzer. As many as 25 samples of human seminal plasma can be analyzed sequentially with CVs of 3.1% for zinc and 1.5% for acid phosphatase. The sensitivity, specificity, and speed of this assay system make it practicable for use in investigation of male infertility.

Characterization of Nuclease Activity in Human Seminal Plasma and its Relationship to Semen Parameters, Sperm DNA Fragmentation and Male Infertility

2016 ◽  
Vol 195 (1) ◽  
pp. 213-219 ◽  
Author(s):  
Alba Fernandez-Encinas ◽  
Agustí García-Peiró ◽  
Jordi Ribas-Maynou ◽  
Carlos Abad ◽  
María José Amengual ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Nuclease Activity ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Human Seminal Plasma ◽  
Sperm Dna

scholarly journals Isolation and Identification of Concanavalin A Binding Glycoproteins from Human Seminal Plasma: A Step Towards Identification of Male Infertility Marker Proteins

2011 ◽  
Vol 31 (6) ◽  
pp. 379-386 ◽  
Author(s):  
Anil Kumar Tomar ◽  
Balwinder Singh Sooch ◽  
Isha Raj ◽  
Sarman Singh ◽  
Tej P. Singh ◽  
...  
Keyword(s):  
Male Infertility ◽  
Seminal Plasma ◽  
Concanavalin A ◽  
Specific Antigen ◽  
Clinical Importance ◽  
Prostatic Acid Phosphatase ◽  
Human Seminal Plasma ◽  
Isolation And Identification ◽  
Altered Expression ◽  
Marker Proteins

Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma.

scholarly journals Human seminal plasma contains somatostatin-64

1994 ◽  
Vol 303 (1) ◽  
pp. 263-268 ◽  
Author(s):  
L Odum ◽  
A H Johnsen
Keyword(s):  
Seminal Plasma ◽  
Biologically Active ◽  
Gel Chromatography ◽  
Human Seminal Plasma ◽  
Active Peptides ◽  
Accessory Sex Glands ◽  
Biologically Active Peptides ◽  
Terminal Amino ◽  
High Concentrations ◽  
Normal Men

Prosomatostatin is processed into two biologically active peptides, somatostatin (SS)-14 and SS-28. SS-14 is found in many tissues, whereas SS-28 is most prominent in the intestine. Human seminal plasma contained high concentrations (median = 3 nmol/l, range 0.6-76 nmol/l, n = 17) of SS with a higher M(r) than that of SS-28 as determined by gel chromatography. After isolation the SS immunoreactivity was identified as SS-64 based on the N-terminal amino acid sequence Leu-Ala-Glu-Leu-Leu-Ser-Glu-Pro-Asn-Gln-, and M(r) of 7242 determined by m.s. and on reactivity with two antibodies raised against SS-14. SS-64 displaced the binding of [125I-Tyr1]SS-14 to rat cerebrocortical membranes as effectively as SS-14 (IC50 = 1.6 mol/l). The concentration of SS-64 in seminal plasma from vasectomized men was significant higher (P < 0.005) than in normal men (median = 25.4 nmol/l, range 1.5-156 nmol/l, n = 21), suggesting that the synthesis of SS-64 takes place in the male accessory sex glands.

scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Cigarette Smoke Condensate ◽  
Human Seminal Plasma ◽  
High Level ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level) were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.

scholarly journals Seminal Plasma Proteomic Biomarkers of Oxidative Stress

2020 ◽  
Vol 21 (23) ◽  
pp. 9113
Author(s):  
Rossella Cannarella ◽  
Andrea Crafa ◽  
Federica Barbagallo ◽  
Laura M. Mongioì ◽  
Rosita A. Condorelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Seminal Fluid ◽  
Accessory Gland ◽  
Male Accessory Gland ◽  
Abnormal Sperm ◽  
Clinical Conditions ◽  
Biomarkers Of Oxidative Stress

The prevalence of idiopathic male infertility is high, up to 75% of patients with abnormal sperm parameters. Hence, the research of its causes is mandatory. Oxidative stress (OS) can be responsible for male infertility in 30–80% of cases. In recent years, seminal plasma (SP) proteomics has developed as a useful tool to provide biomarkers of specific diseases. This systematic review aims to collect the available evidence on the changes of SP proteome in patients exposed to OS to provide possible SP biomarkers of sperm OS. To accomplish this, the following keyterms “seminal fluid proteome”, “seminal plasma proteome”, “oxidative stress”, and “sperm oxidative stress” were used and 137 records were found. Among these, 17 were finally included. Nine proteins involved with OS were found overexpressed in patients with OS. Twenty-three proteins were found differentially expressed in patients with clinical conditions associated with OS, such as varicocele, male accessory gland infection/inflammation, cigarette smoke, and obesity. These proteins do not seem to overlap among the clinical conditions taken into account. We speculate that specific SP proteins may mediate OS in different clinical conditions. Altogether, these results suggest that proteomics could help to better understand some of the molecular mechanisms involved in the pathogenesis of infertility. However, further studies are needed to identify potential biomarkers of male infertility with valuable clinical significance.

Spermatic multinucleation in infertile agriculture workers exposed to carbofuranyl pesticide

1996 ◽  
Vol 54 ◽  
pp. 776-777
Author(s):  
G. Gallegos de L. ◽  
M.M. Arizpe S. ◽  
L.E. Alvarado C. ◽  
E. Ramirez B.
Keyword(s):  
Male Infertility ◽  
Causal Relationship ◽  
Sperm Motility ◽  
Detrimental Effect ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Chemical Agents ◽  
And Function ◽  
Infertile Patients

It is known that the exposure to gonadotoxic agents cause male infertility in agricutural workers. Pesticides provoke a detrimental effect on the spermatogenesis characterized by low count of sprematozoa, as well as sperm motility and morphology alterations. Simultaneosly an increase in FSH seric levels is present.The semen analysis was proposed in 1975 to detect sperm quality and function alteration induced by chemical agents. There are many morphologic anomalies related to the noxious action of some gonadotoxic agents, ie. head deformities, microcephalia, tail defects, etc.We detected in our laboratory a pair of cases of infertile patients, that presented high levels of teratozoospermia with a high percentage of multinucleated spermatozoa and spermatids, and referred exposure to Carbofuranyl (Furadan 300). Since this agent has not been classified as gonadotoxic, we tried to show it's posible causal relationship with spermatic multinucleation phenomenon observed and the fertility of these patients.

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