DNA Metabarcoding of the Leachate Microbiota from Sanitary Landfill: potential for bioremediation process

Abstract Microbial profile knowledge is essential to news alternatives and improvements in current treatments and destinations of landfill leachate that contains a variety of toxic compounds produced by municipal solid waste (MSW) disposal. Environmental DNA metabarcoding is an efficient, quick, and low-cost methodology for whole communities’ characterization. In this respect, the leachate from the Sanitary Landfill of Foz do Iguaçu City showed mixed characteristics from both acid and methanogenic phases, and 16S rDNA metabarcoding suggested the dominance of fermenting bacteria belonging to Firmicutes phylum, followed by Proteobacteria, Bacteroidetes and Synergistetes. The leachate acidogenic phase ended up being masked in the chemical and physical analyzes, however was evidenced in the metabarcoding methodology. On the other hand, no specifically methanogenic group was detected in significant abundance. To future application using culturomics approaches to bioremediation the leachate sample presented groups extensively studied, especially the Pseudomonas genus to heavy metals treatments, such as cadmium.

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COMPARATIVE STUDY BETWEEN FISH COLLECTION BY NATIONAL CENSUS ON RIVER ENVIRONMENT AND ENVIRONMENTAL DNA METABARCODING

Journal of Japan Society of Civil Engineers Ser B1 (Hydraulic Engineering) ◽

10.2208/jscejhe.74.5_i_415 ◽

2018 ◽

Vol 74 (5) ◽

pp. I_415-I_420 ◽

Cited By ~ 1

Author(s):

Yoshihisa AKAMATSU ◽

Takayoshi TSUZUKI ◽

Ryota YOKOYAMA ◽

Yayoi FUNAHASHI ◽

Munehiro OHTA ◽

...

Keyword(s):

Comparative Study ◽

Environmental Dna ◽

Dna Metabarcoding ◽

Fish Collection

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Environmental DNA for functional diversity

10.1093/oso/9780198767220.003.0010 ◽

2018 ◽

Cited By ~ 1

Author(s):

Pierre Taberlet ◽

Aurélie Bonin ◽

Lucie Zinger ◽

Eric Coissac

Keyword(s):

Functional Groups ◽

Functional Diversity ◽

Environmental Dna ◽

Soil Organisms ◽

Meaningful Information ◽

Dna Metabarcoding ◽

Pros And Cons ◽

Target Community

Chapter 10 “Environmental DNA for functional diversity” discusses the potential of environmental DNA to assess functional diversity. It first focuses on DNA metabarcoding and discusses the extent to which this approach can be used and/or optimized to retrieve meaningful information on the functions of the target community. This knowledge usually involves coarsely defined functional groups (e.g., woody, leguminous, graminoid plants; shredders or decomposer soil organisms; pathogenicity or decomposition role of certain microorganisms). Chapter 10 then introduces metagenomics and metatranscriptomics approaches, their advantages, but also the challenges and solutions to appropriately sampling, sequencing these complex DNA/RNA populations. Chapter 10 finally presents several strategies and software to analyze metagenomes/metatranscriptomes, and discusses their pros and cons.

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Environmental DNA

10.1093/oso/9780198767220.001.0001 ◽

2018 ◽

Cited By ~ 137

Author(s):

Pierre Taberlet ◽

Aurélie Bonin ◽

Lucie Zinger ◽

Eric Coissac

Keyword(s):

Graduate Students ◽

Feeding Habits ◽

Environmental Dna ◽

Research Field ◽

Background Information ◽

Ecological Processes ◽

Biodiversity Research ◽

Dna Metabarcoding ◽

Standard Information

Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a particular focus on “eDNA metabarcoding”. Intended for scientists and managers, it provides the background information to allow the design of sound experiments. It revisits all steps necessary to produce high-quality metabarcoding data such as sampling, metabarcode design, optimization of PCR and sequencing protocols, as well as analysis of large sequencing datasets. All these different steps are presented by discussing the potential and current challenges of eDNA-based approaches to infer parameters on biodiversity or ecological processes. The last chapters of this book review how DNA metabarcoding has been used so far to unravel novel patterns of diversity in space and time, to detect particular species, and to answer new ecological questions in various ecosystems and for various organisms. Environmental DNA for biodiversity research and monitoring constitutes an essential reading for all graduate students, researchers and practitioners who do not have a strong background in molecular genetics and who are willing to use eDNA approaches in ecology and biomonitoring.

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Estimating aquatic plant diversity and distribution in rivers from Jingjinji Region, China, using environmental DNA metabarcoding and a traditional survey method

Environmental Research ◽

10.1016/j.envres.2021.111348 ◽

2021 ◽

pp. 111348

Author(s):

Fenfen Ji ◽

Liang Yan ◽

Saihong Yan ◽

Tianlong Qin ◽

Jianzhong Shen ◽

...

Keyword(s):

Plant Diversity ◽

Aquatic Plant ◽

Environmental Dna ◽

Survey Method ◽

Dna Metabarcoding

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Environmental DNA metabarcoding as a useful tool for evaluating terrestrial mammal diversity in tropical forests

Ecological Applications ◽

10.1002/eap.2335 ◽

2021 ◽

Author(s):

José Luis Mena ◽

Hiromi Yagui ◽

Vania Tejeda ◽

Emilio Bonifaz ◽

Eva Bellemain ◽

...

Keyword(s):

Tropical Forests ◽

Environmental Dna ◽

Terrestrial Mammal ◽

Dna Metabarcoding ◽

Mammal Diversity

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Sifting environmental DNA metabarcoding data sets for rapid reconstruction of marine food webs

Fish and Fisheries ◽

10.1111/faf.12553 ◽

2021 ◽

Author(s):

Simone D’Alessandro ◽

Stefano Mariani

Keyword(s):

Food Webs ◽

Environmental Dna ◽

Data Sets ◽

Marine Food Webs ◽

Dna Metabarcoding ◽

Marine Food

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Corrigendum to: PEMA: a flexible pipeline for environmental DNA metabarcoding analysis of the 16S/18S ribosomal RNA, ITS, and COI marker genes

GigaScience ◽

10.1093/gigascience/giaa150 ◽

2020 ◽

Vol 9 (12) ◽

Author(s):

Haris Zafeiropoulos ◽

Ha Quoc Viet ◽

Katerina Vasileiadou ◽

Antonis Potirakis ◽

Christos Arvanitidis ◽

...

Keyword(s):

Ribosomal Rna ◽

Environmental Dna ◽

Marker Genes ◽

18S Ribosomal Rna ◽

Dna Metabarcoding

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Chitosan-Based Nanocomposite Polymeric Membranes for Water Purification—A Review

Materials ◽

10.3390/ma14092091 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2091

Author(s):

Angela Spoială ◽

Cornelia-Ioana Ilie ◽

Denisa Ficai ◽

Anton Ficai ◽

Ecaterina Andronescu

Keyword(s):

Water Purification ◽

Antioxidant Activities ◽

Low Cost ◽

Synthetic Polymers ◽

Water Systems ◽

Polymeric Membranes ◽

Wastewater Purification ◽

Toxic Compounds ◽

The Past ◽

Materials Used

During the past few years, researchers have focused their attention on developing innovative nanocomposite polymeric membranes with applications in water purification. Natural and synthetic polymers were considered, and it was proven that chitosan-based materials presented important features. This review presents an overview regarding diverse materials used in developing innovative chitosan-based nanocomposite polymeric membranes for water purification. The first part of the review presents a detailed introduction about chitosan, highlighting the fact that is a biocompatible, biodegradable, low-cost, nontoxic biopolymer, having unique structure and interesting properties, and also antibacterial and antioxidant activities, reasons for using it in water treatment applications. To use chitosan-based materials for developing nanocomposite polymeric membranes for wastewater purification applications must enhance their performance by using different materials. In the second part of the review, the performance’s features will be presented as a consequence of adding different nanoparticles, also showing the effect that those nanoparticles could bring on other polymeric membranes. Among these features, pollutant’s retention and enhancing thermo-mechanical properties will be mentioned. The focus of the third section of the review will illustrate chitosan-based nanocomposite as polymeric membranes for water purification. Over the last few years, researchers have demonstrated that adsorbent nanocomposite polymeric membranes are powerful, important, and potential instruments in separation or removal of pollutants, such as heavy metals, dyes, and other toxic compounds presented in water systems. Lastly, we conclude this review with a summary of the most important applications of chitosan-based nanocomposite polymeric membranes and their perspectives in water purification.

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