scholarly journals HTLV screening of blood donors using chemiluminescence immunoassay in three major provincial blood centers of China

2020 ◽  
Author(s):  
Junpeng Zhao ◽  
Feixue Zhao ◽  
Wei Han ◽  
Xiaoxuan Xu ◽  
Lilin Wang ◽  
...  
Keyword(s):  
Blood Donors ◽  
Safety Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chemiluminescence Immunoassay ◽  
Blood Donations ◽  
High School Degree ◽  
Blood Center ◽  
Human T Cell ◽  
Confirmatory Tests ◽  
Blood Screening

Abstract Background: Human T-cell lymphotropic virus (HTLV) remains a major safety concern for blood supplies. Despite many HTLV positive cases being reported in southeastern China, the detection of HTLV has not been prioritized in routine blood screening. Additionally, data on the prevalence of HTLV infection among blood donors is also limited. The objective of this study was to investigate the prevalence of HTLV among blood donors in three Chinese provinces through their representative blood centers, to evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening.Methods: From November 2018 to March 2019, blood plasma samples were collected from Hebei, Changsha, and Shenzhen blood centers and were screened for the HTLV-1/2 antibody using a CLIA and enzyme-linked immunosorbent assay (ELISA). This was followed by confirmatory tests using INNO-LIA HTLV I/II.Results: A total of 59,929 blood donations were collected and screened for HTLV-1/2. The reactive rate of CLIA and ELISA among donations in the Shenzhen blood center (0.0943%, 27/28, 621) was higher than Hebei (0.0248%, 4/16,144), and Changsha (0.0198%, 3/15,164) (p < 0.05). After confirmation, 3 samples were confirmed as indeterminate for HTLV antibodies, and only one sample from the Shenzhen blood center was confirmed as HTLV-1. The overall prevalence of HTLV-1/2 was 1.67 per 100 000 (1/59,929). The HTLV-infected blood came from a 32-year-old first-time female donor with a high school degree, who belonged to the SHE ethnic minority and was born in the Fujian province.Conclusions: In summary, the overall prevalence of HTLV-1/2 among blood donors in the three blood centers in China remains relatively low. However, blood donations with positive or indeterminate results for HTLV antibodies reminded us of the importance of HTLV screening among blood donors in China.

scholarly journals HTLV screening of blood donors using chemiluminescence immunoassay in three major provincial blood centers of China

2020 ◽  
Author(s):  
Junpeng Zhao ◽  
Feixue Zhao ◽  
Wei Han ◽  
Xiaoxuan Xu ◽  
Lilin Wang ◽  
...  
Keyword(s):  
Blood Donors ◽  
Safety Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chemiluminescence Immunoassay ◽  
Blood Donations ◽  
High School Degree ◽  
Blood Center ◽  
Human T Cell ◽  
Confirmatory Tests ◽  
Blood Screening

Abstract Background: Human T-cell lymphotropic virus (HTLV) remains a major safety concern for blood supplies. Despite many HTLV positive cases being reported in southeastern China, the detection of HTLV has not been prioritized in routine blood screening. Additionally, data on the prevalence of HTLV infection among blood donors is also limited.Objective: The objective of this study was to investigate the prevalence of HTLV among blood donors in three Chinese provinces through their representative blood centers, to evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening.Methods: From November 2018 to March 2019, blood plasma samples were collected from Hebei, Changsha, and Shenzhen blood centers and were screened for the HTLV-1/2 antibody using a CLIA and enzyme-linked immunosorbent assay (ELISA). This was followed by confirmatory tests using INNO-LIA HTLV I/II.Results: A total of 59,929 blood donations were collected and screened for HTLV-1/2. The reactive rate of CLIA and ELISA among donations in the Shenzhen blood center (0.0943%, 27/28, 621) was higher than Hebei (0.0248%, 4/16,144), and Changsha (0.0198%, 3/15,164) (p < 0.05). After confirmation, 3 samples were confirmed as indeterminate for HTLV antibodies, and only one sample from the Shenzhen blood center was confirmed as HTLV-1. The overall prevalence of HTLV-1/2 was 1.67 per 100 000 (1/59 929). The HTLV-infected blood came from a 32-year-old first-time female donor with a high school degree, who belonged to the SHE ethnic minority and was born in the Fujian province.Conclusion: In summary, the overall prevalence of HTLV-1/2 among blood donors in the three blood centers in China remains relatively low. However, blood donations with positive or indeterminate results for HTLV antibodies reminded us of the importance of HTLV screening among blood donors in China.

scholarly journals HTLV screening of blood donors using chemiluminescence immunoassay in three major provincial blood centers of China

2020 ◽  
Author(s):  
Junpeng Zhao ◽  
Feixue Zhao ◽  
Wei Han ◽  
Xiaoxuan Xu ◽  
Lilin Wang ◽  
...  
Keyword(s):  
Blood Donors ◽  
Safety Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Chemiluminescence Immunoassay ◽  
Blood Donations ◽  
High School Degree ◽  
Blood Center ◽  
Human T Cell ◽  
Blood Screening

Abstract Background Human T-cell lymphotropic virus (HTLV) remains a major safety concern for blood supplies. Despite many HTLV positive cases being reported in southeastern China, the detection of HTLV has not been prioritized in routine blood screening. Additionally, data on the prevalence of HTLV infection among blood donors is also limited. Objective: The objective of this study was to investigate the prevalence of HTLV among blood donors in three Chinese provinces through their representative blood centers, to evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening. Methods: From November 2018 to March 2019, blood plasma samples were collected from Hebei, Changsha, and Shenzhen blood centers and were screened for the HTLV-1/2 antibody using a CLIA and enzyme-linked immunosorbent assay (ELISA). This was followed by confirmatory tests using INNO-LIA HTLV I/II. Results: A total of 59,929 blood donations were collected and screened for HTLV-1/2. The reactive rate of CLIA and ELISA among donations in the Shenzhen blood center (0.0943%, 27/28, 621) was higher than Hebei (0.0248%, 4/16,144), and Changsha (0.0198%, 3/15,164) ( p < 0.05). After confirmation, 3 samples were confirmed as indeterminate for HTLV antibodies, and only one sample from the Shenzhen blood center was confirmed as HTLV-1. The overall prevalence of HTLV-1/2 was 1.67 per 100 000 (1/59 929). The HTLV-infected blood came from a 32-year-old first-time female donor with a high school degree, who belonged to the SHE ethnic minority and was born in the Fujian province. Conclusion: In summary, the overall prevalence of HTLV-1/2 among blood donors in the three blood centers in China remains relatively low. However, blood donations with positive or indeterminate results for HTLV antibodies reminded us of the importance of HTLV screening among blood donors in China.

scholarly journals HTLV screening using chemiluminescence immunoassay among blood donors in three blood centers in China

2020 ◽  
Author(s):  
Junpeng Zhao ◽  
Feixue Zhao ◽  
Wei Han ◽  
Xiaoxuan Xu ◽  
Lilin Wang ◽  
...  
Keyword(s):  
Blood Donors ◽  
Confirmatory Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Chemiluminescence Immunoassay ◽  
Blood Donations ◽  
High School Degree ◽  
Blood Center ◽  
Human T Cell ◽  
Blood Screening

Abstract Background Human T-cell lymphotropic virus (HTLV) remains a concern for blood safety. The detection of HTLV has not yet been applied for routine blood screening in China, even though lots of HTLV positive cases have been reported in southeastern China. However, data on the prevalence of HTLV infection among blood donors is limited. Objective The objective was to investigate the prevalence of HTLV among blood donors in three representative blood centers in China and evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening. Methods From November 2018 to March 2019, blood plasma samples collected from Hebei, Changsha and Shenzhen blood centers were screening for HTLV-1/2 antibody using Lumipulse G HTLV-I/II Immunoreaction Cartridges set (CLIA) and enzyme-linked immunosorbent assay (ELISA), followed with confirmatory test using INNO-LIA HTLV I/II. Results A total of 59929 blood donations were collected and screened for HTLV-1/2. The reactive rate of CLIA and ELISA among donations in Shenzhen blood center (0.0943%, 27/28621) was higher than Hebei (0.0248%, 4/16144) and Changsha (0.0198%, 3/15164) ( P <0.05). After confirmation, 3 samples were confirmed as indeterminate for HTLV antibodies, only 1 sample from Shenzhen blood center was confirmed and typed as HTLV-1. The overall prevalence of HTLV-1/2 was 1.67 per 100,000 (1/59929). The HTLV-infected blood donor was a first female donor at age 32 with high school degree, born in Fujian province, and SHE minority. Conclusion In summary, the overall prevalence of HTLV-1/2 among blood donors in the tree blood centers in China remains relatively low. However, blood donations with positive or indeterminate results for HTLV antibodies found in the study reminded us the importance of HTLV screening among blood donors in China. In the view of HTLV prevalence and cost, all the first-blood donors should undergo HTLV screening as a systematic strategy to reduce the risk of transmission of HTLV through blood transfusion.

scholarly journals Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Patience Uchenna Tweteise ◽  
Bernard Natukunda ◽  
Joel Bazira
Keyword(s):  
T Cell ◽  
Blood Donors ◽  
Spastic Paraparesis ◽  
Cross Sectional Study ◽  
Blood Bank ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Adult T Cell Leukemia ◽  
Human T Cell ◽  
Western Uganda

Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2) antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA). Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2%) males and 139 (37.8%) females), only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

Human T-Cell Lymphotropic Virus Antibody Screening: Data Survey on 33,603 German Blood Donors Correlated to Confirmatory Tests

Vox Sanguinis ◽  
1985 ◽  
Vol 49 (5) ◽  
pp. 327-330 ◽  
Author(s):  
P. Kühnl ◽  
S. Seidl ◽  
R. Kurth ◽  
J. Lower ◽  
H.-D. Brede ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Donors ◽  
Virus Antibody ◽  
Antibody Screening ◽  
Human T Cell ◽  
Confirmatory Tests ◽  
Data Survey

scholarly journals Seroprevalence of human T-cell lymphotropic virus I and II (HTLV I/II) among blood donors in a public blood center of Sergipe State, northeastern Brazil

2019 ◽  
Vol 31 (3) ◽  
pp. 96-101
Author(s):  
Cibele Macedo Santos ◽  
◽  
Rafael de Souza Aguiar ◽  
Ester Bencz ◽  
Vanessa Oliveira Amorim ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Donors ◽  
Northeastern Brazil ◽  
Blood Center ◽  
Human T Cell

scholarly journals BRIEF OVERVIEW ON HEPATITIS C VIRUS IMMUNOASSAYS

2016 ◽  
Vol 4 (1) ◽  
pp. 178-184
Author(s):  
Rajesh Sharma ◽  
Pankaj Sharma ◽  
Gaush Talat ◽  
Praveen Gautam ◽  
Reba Chhabra ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Donor Blood ◽  
Chemiluminescence Immunoassay ◽  
Window Period ◽  
Sensitivity Specificity ◽  
Blood Screening

The publication deals with a brief overview of Hepatitis C Virus (HCV) and donor blood screening for HCV by using conventional Rapid, Enzyme Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) also. The advantages of various generation of HCV tests in terms of sensitivity, specificity and reduction in window period are discussed.

Human T-Cell Lymphotropic Virus Antibody Screening: Data Survey on 33,603 German Blood Donors Correlated to Confirmatory Tests

Vox Sanguinis ◽  
1985 ◽  
Vol 49 (5) ◽  
pp. 327-330
Author(s):  
P. Kühnl ◽  
S. Seidl ◽  
R. Kurth ◽  
J. Löwer ◽  
H.-D. Brede ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Donors ◽  
Virus Antibody ◽  
Antibody Screening ◽  
Human T Cell ◽  
Confirmatory Tests ◽  
Data Survey

scholarly journals Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Le Chang ◽  
Junpeng Zhao ◽  
Fei Guo ◽  
Huimin Ji ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Blood Donors ◽  
Screening Strategy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hiv Positive ◽  
Plasma Samples ◽  
Serological Method ◽  
Clinical Laboratories ◽  
Electrochemiluminescence Immunoassay ◽  
Blood Screening

Background. Enzyme-linked immunosorbent assay (ELISA) is the only serological method approved for blood screening in China. Automated chemiluminescence immunoassay (CLIA) and electrochemiluminescence immunoassay (ECLIA) had been used in clinical laboratories but not applied to screen HIV among blood donors. This study aimed to evaluate the performance of ELISA, CLIA, and ECLIA, focusing on the feasibility of CLIA/ECLIA for blood screening. Method. 1029 blood donations from 14 blood centers screened by ELISA were enrolled in the study. All plasma samples were tested by eight ELISA assays in 16 blood centers, followed by the detection of CLIA and ECLIA methods in the National Center for Clinical Laboratories (NCCL), further confirmed by nucleic acid testing (NAT) and Western blot (WB). Results. Of 1029 samples, 136 were confirmed as HIV positive. CLIA and ECLIA assay had similar sensitivities with ELISAs but showed higher specificity (CLIA: 99.1%, 885/893; ECLIA: 99.0%, 884/893), concordance rate (CLIA: 99.2%, 1021/1029; ECLIA: 99.1%, 1020/1029), and positive predictive value (PPV) (CLIA: 94.4%, 136/144; ECLIA: 93.8%, 136/145) than most of ELISA kits (>5 ELISAs) (P<0.05). Kappa values of CLIA (0.967) and ECLIA (0.963) were the highest among all the serologic assays. Among 451 samples with initial ELISA reactivity, 315 were negatives, of which 307 (97.5%) and 306 (97.1%) were detected as nonreactive by CLIA (8 nonspecific reactions) and ECLIA (9 nonspecific reactions), respectively. Conclusion. Compared with ELISA, CLIA and ECLIA are more specific and accurate in detecting HIV antibody/antigen and can keep more nonspecifically reactive donors detected by ELISA. CLIA and ECLIA can be used for the improvement of serological blood screening strategy to avoid the unnecessary loss of blood donors.

scholarly journals Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors

2010 ◽  
Vol 43 (2) ◽  
pp. 111-115 ◽  
Author(s):  
Rafaela Gomes Andrade ◽  
Maísa Aparecida Ribeiro ◽  
Maria Sueli Silva Namen-Lopes ◽  
Sônia Mara Nunes Silva ◽  
Fernando Valadares Basques ◽  
...  
Keyword(s):  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Blood Donors ◽  
High Rate ◽  
Confirmatory Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human T Cell ◽  
Blood Banks ◽  
Pcr Assays

INTRODUCTION: HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. METHODS: Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals). RESULTS: The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases. CONCLUSIONS: Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB.

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