scholarly journals Current standart protocols of RT-PCR of nasopharyngeal swab in neonatal patient is not sensitive enough for determination of vertical transmission

2021 ◽  
Author(s):  
Maria Alice Fusco ◽  
Vinícius Mantini ◽  
Jarba S. S. Júnior ◽  
Raphael G. C. Gomes ◽  
Camilla G. Salmont ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Vertical Transmission ◽  
Umbilical Cord Blood ◽  
Nasopharyngeal Swab ◽  
Transplacental Transmission ◽  
Double Positive ◽  
Neonatal Patient ◽  
Naval Hospital

Abstract The pandemic experienced in recent months has raised questions that should be investigate in the clinical practice. Transplacental transmission of SARS-CoV-2 and the consequences to the fetus and newborn have called attention due to the increasing number of infections, contradicting previous evidences that there was no possibility of coronavirus transmission from the mother to the fetus. In this work we showed three cases of pregnant women with positive SARS-CoV-2 antibodies serology on admission in Naval Hospital Marcílio Dias (HNMD), Rio de Janeiro, Brazil. Samples of umbilical cord blood was double positive (IgM and IgG) for one patient, double negative for one patient and positive for IgG and negative for IgM for third patient. Maternal and neonatal nasopharyngeal swab samples analyzed by PCR for SARS-CoV-2 was positive for two maternal patients and negative for all newborns tested. It was possible to detect the SARS-CoV-2 in amniotic fluid and umbilical cord blood using the nested-PCR technics, thus being successfully evidenced transplacental transmission. We suggested that nasopharyngeal swab PCR test of neonates does not have a correlation with vertical transmission and thus, this molecular test is not useful for investigation of transplacental infection.

scholarly journals Current standard protocols of RT-PCR of nasopharyngeal swab in neonatal patient is not sensitive enough for determination of vertical transmission

2021 ◽  
Author(s):  
Maria Alice Fusco ◽  
Vinícius Mantini ◽  
Jarba S. S. Júnior ◽  
Raphael G. C. Gomes ◽  
Camilla G. Salmont ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Vertical Transmission ◽  
Umbilical Cord Blood ◽  
Nasopharyngeal Swab ◽  
Transplacental Transmission ◽  
Current Standard ◽  
Double Positive ◽  
Neonatal Patient ◽  
Naval Hospital

Abstract The pandemic experienced in recent months has raised questions that should be investigate in the clinical practice. Transplacental transmission of SARS-CoV-2 and the consequences to the fetus and newborn have called attention due to the increasing number of infections, contradicting previous evidences that there was no possibility of coronavirus transmission from the mother to the fetus. We presented three cases of pregnant women with positive SARS-CoV-2 antibodies serology on admission in Naval Hospital Marcílio Dias (HNMD), Rio de Janeiro, Brazil. Samples of umbilical cord blood was double positive (IgM and IgG) for one patient, double negative for one patient and positive for IgG and negative for IgM for third patient. Maternal and neonatal nasopharyngeal swab samples analyzed by PCR for SARS-CoV-2 was positive for two maternal patients and negative for all newborns tested. It was possible to detect the SARS-CoV-2 in amniotic fluid and umbilical cord blood using the nested-PCR technics, thus being successfully evidenced transplacental transmission. We suggested that nasopharyngeal swab PCR test of neonates does not have a correlation with vertical transmission and thus, this molecular test is not useful for investigation of transplacental infection.

scholarly journals Relationship between expression of HBV-infection markers in placenta and clinical data of mothers and their newborns

2021 ◽  
Vol 13 (2) ◽  
pp. 95-101
Author(s):  
M. A. Belopolskaya ◽  
V. E. Karev ◽  
V. Yu. Avrutin ◽  
A. V. Dmitriev ◽  
D. A. Gusev
Keyword(s):  
Viral Load ◽  
Hepatitis B ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Vertical Transmission ◽  
Umbilical Cord Blood ◽  
Structural Changes ◽  
Antiviral Treatment ◽  
Undetectable Viral Load ◽  
Hbv Infection

Vertical transmission of viral hepatitis B (HBV) is one of the main transmission ways in endemic regions. There is a relationship between structural changes in the placenta and the clinical course of HBV infection. The risk of vertical transmission of hepatitis B depends on the condition of the placenta.The goal of this work was to study the relationship between HBsAg and / or HBcorAg expression in placenta on the one hand, and clinical parameters of mothers and newborns on the other hand.The study included 19 patients with CHB who gave birth at the Botkins infectious disease hospital in 2019. The first group included patients with markers of HBV infection (HBsAg and / or HBcorAg) detectable via immunohistochemical examination in at least one of the layers of the placenta. In patients from the second group, no markers of HBV infection were detected in any layer of the placenta. Inflammatory changes in placenta were detected in all women from the first group and in 9 (64%) women from the second group. HBsAg in umbilical cord blood was detected in 3 (16%) newborns. In two patients, whose children had HBsAg in the umbilical cord blood, no markers of HBV infection were detected in placenta. In the first group, only one newborn had HBsAg in umbilical cord blood. In two patients receiving an antiviral treatment during pregnancy, markers of HBV infection were identified in the placenta. Markers of HBV infection in placenta were also detected in women with undetectable viral load.ConclusionsMarkers of HBV infection can be found in placenta at low maternal viral load.HBsAg in umbilical cord blood can be detected even in patients with no markers of HBV infection in placenta.Antiviral treatment during pregnancy does not prevent the possibility of placenta’s infection with HBV.

scholarly journals Determination of lead levels in maternal and umbilical cord blood at birth at the Lagos University Teaching Hospital, Lagos

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0211535 ◽  
Author(s):  
Jejelola I. Ladele ◽  
Iretiola Bamikeolu Fajolu ◽  
Veronica Chinyere Ezeaka
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Teaching Hospital ◽  
Umbilical Cord Blood ◽  
University Teaching ◽  
Lead Levels

scholarly journals Analysis of SARS-CoV-2 vertical transmission during pregnancy

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Claudio Fenizia ◽  
Mara Biasin ◽  
Irene Cetin ◽  
Patrizia Vergani ◽  
Davide Mileto ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Vertical Transmission ◽  
Umbilical Cord Blood ◽  
Inflammatory Responses ◽  
Vaginal Mucosa ◽  
Cord Plasma ◽  
Expression Of Genes ◽  
Obstetric Management ◽  
The Impact

Abstract The impact of SARS-CoV-2 infection during gestation remains unclear. Here, we analyse the viral genome on maternal and newborns nasopharyngeal swabs, vaginal swabs, maternal and umbilical cord plasma, placenta and umbilical cord biopsies, amniotic fluids and milk from 31 mothers with SARS-CoV-2 infection. In addition, we also test specific anti-SARS-CoV-2 antibodies and expression of genes involved in inflammatory responses in placentas, and in maternal and umbilical cord plasma. We detect SARS-CoV-2 genome in one umbilical cord blood and in two at-term placentas, in one vaginal mucosa and in one milk specimen. Furthermore, we report the presence of specific anti-SARS-CoV-2 IgM and IgG antibodies in one umbilical cord blood and in one milk specimen. Finally, in the three documented cases of vertical transmission, SARS-CoV-2 infection was accompanied by a strong inflammatory response. Together, these data support the hypothesis that in utero SARS-CoV-2 vertical transmission, while low, is possible. These results might help defining proper obstetric management of COVID-19 pregnant women, or putative indications for mode and timing of delivery.

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