Dendrimer-Modified Gold Nanorods As A Platform For Combinational Gene Therapy And Photothermal Therapy of Tumors

Abstract Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. Methods: We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are performed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer.Results: The results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection. Conclusion: Our findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor.

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Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors

Journal of Experimental & Clinical Cancer Research ◽

10.1186/s13046-021-02105-3 ◽

2021 ◽

Vol 40 (1) ◽

Author(s):

Lili Ye ◽

Yaoming Chen ◽

Jizong Mao ◽

Xiaotian Lei ◽

Qian Yang ◽

...

Keyword(s):

Gene Therapy ◽

Colon Cancer ◽

Cancer Cells ◽

Gold Nanorods ◽

Photothermal Therapy ◽

Transfection Efficiency ◽

Single Mode ◽

Ct Imaging ◽

Colon Cancer Cells ◽

Proliferation And Apoptosis

Abstract Background The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. Methods We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer. Results The results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection. Conclusion Our findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor.

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Dendrimer-modified Gold Nanorods as a Platform for Combinational Gene Therapy and Photothermal Therapy of Tumors

10.21203/rs.3.rs-263824/v1 ◽

2021 ◽

Author(s):

Lili Ye ◽

Yaoming Chen ◽

Jizong Mao ◽

Xiaotian Lei ◽

Qian Yang ◽

...

Keyword(s):

Gene Therapy ◽

Cancer Cells ◽

Gold Nanorods ◽

Thermal Imaging ◽

Photothermal Therapy ◽

Tumor Imaging ◽

Transfection Efficiency ◽

Single Mode ◽

Delivery Vector ◽

Tail Vein Injection

Abstract Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au NR@PAMAM-GX1) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.Conclusion: The synthesized Au NR@PAMAM-GX1 is a potential nanoplatform for tumor imaging and treatment.

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Enzyme-conjugated gold nanoparticles for combined enzyme and photothermal therapy of colon cancer cells

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2019.04.019 ◽

2019 ◽

Vol 572 ◽

pp. 333-344 ◽

Cited By ~ 17

Author(s):

Mostafa Akbarzadeh Khiavi ◽

Azam Safary ◽

Ayuob Aghanejad ◽

Jaleh Barar ◽

Seyed Hossein Rasta ◽

...

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Cancer Cells ◽

Photothermal Therapy ◽

Colon Cancer Cells

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Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells

Oncology Letters ◽

10.3892/ol.2018.9857 ◽

2018 ◽

Cited By ~ 5

Author(s):

Gang Zhou ◽

Jing Yang ◽

Peng Song

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

Mapk Signaling ◽

Mapk Signaling Pathway ◽

Colon Cancer Cells ◽

Erk Mapk ◽

Proliferation And Apoptosis

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Effects of cyclin-dependent kinase 8 specific siRNA on the proliferation and apoptosis of colon cancer cells

Journal of Experimental & Clinical Cancer Research ◽

10.1186/1756-9966-30-109 ◽

2011 ◽

Vol 30 (1) ◽

pp. 109 ◽

Cited By ~ 18

Author(s):

Song-Bing He ◽

Yin Yuan ◽

Lei Wang ◽

Min-Jing Yu ◽

Yi-Bei Zhu ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Cyclin Dependent Kinase ◽

Colon Cancer Cells ◽

Proliferation And Apoptosis

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MiR-125b Inhibits Cell Proliferation and Induces Apoptosis in Human Colon Cancer SW480 Cells via Targeting STAT3

Recent Patents on Anti-Cancer Drug Discovery ◽

10.2174/1574892816666210708165037 ◽

2021 ◽

Vol 16 ◽

Author(s):

Junhe Zhang ◽

Wenwen Yang ◽

Yunxi Xiao ◽

Linlin Shan

Keyword(s):

Colon Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Luciferase Reporter ◽

Colon Cancer Cells ◽

Luciferase Reporter Gene ◽

Luciferase Reporter Gene Assay ◽

Gene Assay ◽

Proliferation And Apoptosis ◽

Sw480 Cells

Background: Colon cancer is one of the most common types of cancer worldwide. Multiple studies have unveiled the key role of microRNAs (miRNAs) in the development of various types of cancer. However, the mechanism of action of miR-125b in the development and progression of colon cancer remains unknown. Objective: In this study, we explored the association of miR-125b and signal transducer and activator of transcription 3 (STAT3) and its role in the proliferation and apoptosis of SW480 colon cancer cells. Methods: The miR-125b expression in NCM460, SW480, HT29, and HCT8 cells was detected using quantitative real-time polymerase chain reaction (qRT-PCR). SW480 cells were transfected with lentiviruses of GFP–miR–125b and GFP–NC to establish a stable miR-125b overexpression colon cancer cell model and a control model. The targeting relationship between miR-125b and STAT3 was analyzed using bioinformatics and verified by the dual-luciferase reporter gene assay. Cell proliferation and apoptosis were assessed using the Cell Counting Kit-8 assay and TUNEL staining. The expression levels of STAT3, Bcl-2, and Bax were analyzed using Western blot analysis. Results: It was found that the relative mRNA expression of miR-125b was decreased in SW480, HT29, and HCT8 cells compared with that in NCM460 cells (P<0.05). The luciferase reporter gene assay confirmed that miR-125b downregulated the STAT3 gene expression (P<0.05). Overexpression of miR-125b inhibited proliferation and promoted apoptosis in SW480 colon cancer cells and was accompanied by upregulated Bax expression and downregulated Bcl-2 expression (P<0.05). Re-expression of STAT3 promoted cell proliferation and inhibited cell apoptosis, whereas Bcl-2 expression increased, and Bax expression decreased (P<0.05). Conclusion: The miR-125b regulates the expression of Bax and Bcl-2 by downregulating the expression of STAT3, thereby inhibiting proliferation and inducing apoptosis of SW480 colon cancer cells.

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The Role of Interleukin-22 Receptor1 in the Proliferation and Apoptosis of Colonrectal Cancer

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2655 ◽

2021 ◽

Vol 11 (1) ◽

pp. 22-27

Author(s):

Xiaoning Qin ◽

Hongxun Ruan ◽

Yinghao Hao ◽

Weiqi Kong ◽

Jing Zhao ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Interleukin 22 ◽

Nuclear Antigens ◽

Proliferation And Apoptosis ◽

Colorectal Cancer Cells ◽

Before And After ◽

And Control

Background: To study the relationship between interleukin-22 receptor1 (IL-22R1) and the proliferation and apoptosis of colon cancer cells. Methods: SW480, SW620 (Human Colorectal Cancer Cell Lines) that express positive to IL-22R1 were exposed in the environment of IL-22. The proliferation trial included 5 groups: IL-22, 5-FU, 5-FU + IL-22, medium and control. The apoptosis trial included 4 groups: IL-22, 5-FU, 5-FU + IL-22 and control. The result of apoptosis was detected by Apoptosis Kit (AnnxinVPE and 7-AAD), and proliferation was detected by Ki67 antibody (Cell proliferation-associated nuclear antigens). The rates of proliferation and apoptosis were detected by flow cytometry. Changes of the rate of proliferation and apoptosis before and after silencing were compared and analyzed statistically after silencing the gene of IL-22R1. Result: The combination of IL-22R1 and IL-22 could significantly inhibit the apoptosis of colon cancer cells and promote the proliferation of colon cancer cells (P < 0.05). The effect was significantly weakened when IL-22R1 was silenced (P < 0.05). Conclusion: IL-22R1 combined with IL-22 could promote the proliferation and inhibit apoptosis of colorectal cancer cells. In addition, blocking IL-22R1 could eliminate the influence of IL-22 on the proliferation and apoptosis of colorectal cancer cells.

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