LC-ESI-MS/GC-MS Based Metabolite Profiling of Chlorophytum comosum (Thunb.) Jaques and evaluation of its antioxidant and antiproliferative effects on lung and breast cancer cell lines

Abstract Background Chlorophytum comosum popularly known as Spider Ivy is an important medicinal plant in traditional Chinese medicine utilized in the treatment of many ailments, however its detailed chemical composition and biological activity is not much explored. The present study aims to identify different chemical constituents present in roots and leaves of Chlorophytum comosum and investigates its antioxidant, antiproliferative and haemolytic effects on breast (MCF-7) and lung cancer cell lines (A549, H1299) as compared to normal lung (L-132) cell lines. Methods Chemical constituents from aqueous roots and leaves extracts were identified using LC-ESI-MS/GC-MS. The identified compounds were annotated based on match of mass spectral database with the literature using NIST 14 and METLIN databases. Antioxidant activity was checked using DPPH, FRAP and TPC assays. The antiproliferative effects of ethanolic roots and leaf extracts of Chlorophytum comosum were measured by MTT assay on breast (MCF-7), lung cancer (A549 & H1299) and normal lung (L-132) cell lines. The toxicity studies of the extracts were carried out using Haemolytic assay. Results GC-MS analysis identified 34 new metabolites in roots and 17 from leaves, while as 17 compounds from roots and 7 from leaves were detected by LC-ESI-MS. Significant antiproliferative effects were observed on MCF-7 & A549 cell lines with IC50 values ranging from 31.94 µg/ml to 77.84 µg/ml while no marked response was observed against normal cell line. Haemolysis studies revealed no significant toxicity of the extracts towards the biological system. Conclusion Our study is the first preliminary report on the detailed chemical composition and antiproliferative potential of Chlorophytum comosum, indicating significant specific antiproliferative activities against lung (A549) and breast (MCF-7) cancer cell lines. However, further studies are required to understand the mechanism involved in cytotoxic properties of Chlorophytum comosum.

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Chemical Profiling of Chlorophytum comosum (Thunb.) Jaques by GCMS/LC-ESI-MS and its Antiproliferative Effects on Human Carcinoma Cell Lines

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666201123085300 ◽

2020 ◽

Vol 20 ◽

Author(s):

Shehla Adhami ◽

Humaira Farooqi ◽

Malik Z. Abdin ◽

Ram Prasad ◽

Asrar A. Malik

Keyword(s):

Breast Cancer ◽

Lung Cancer ◽

Chemical Composition ◽

Cell Lines ◽

Normal Lung ◽

Antiproliferative Effects ◽

Esi Ms ◽

Chlorophytum Comosum ◽

Detailed Chemical ◽

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Background: Chlorophytum comosum popularly known as Spider Ivy is used as medicinal plant in traditional Chinese medicine, however its detailed chemical composition and biological activity is yet unexplored. Objective: To carry out phytochemical investigation on different parts of Chlorophytum comosum using GC-MS/LC-ESI-MS and evaluation of its antioxidant, haemolytic and antiproliferative potential on breast cancer (MCF-7), lung cancer (A549, H1299) and normal lung (L-132) cell lines. Methods: Chemical constituents from aqueous roots and leaves extracts were identified using LC-ESI-MS/GC-MS. The identified compounds were annotated based on match of mass spectra with the literature using NIST 14 and METLIN databases. Antioxidant activity was checked using DPPH, FRAP and TPC assays. The antiproliferative effects of ethanolic roots and leaves extracts of Chlorophytum comosum were measured by MTT assay on breast cancer (MCF-7), lung cancer (A549 & H1299) and normal lung (L-132) cell lines. The toxicity studies of the extracts were carried out using Haemolysis assay. Results: GC-MS analysis identified 34 metabolites in roots and 17 from leaves, while as 17 compounds from roots and 7 from leaves were detected by LC-ESI-MS. Significant antiproliferative effects were observed on the A549 and MCF-7 cancer cell lines with IC50 values ranging from 56.86 µg/ml to 68.68 µg/ml while no marked response was observed against normal cell line L-132. Conclusion: Our study represents the first report on the detailed chemical composition and antiproliferative potential of Chlorophytum comosum against lung and breast cancer cell lines.

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Chemical Constituents from Walsura pinnata (Meliaceae)

Natural Product Communications ◽

10.1177/1934578x1701200905 ◽

2017 ◽

Vol 12 (9) ◽

pp. 1934578X1701200

Author(s):

Mohamad A. Mahdzir ◽

Jamil A. Shilpi ◽

Norfaizah Mahmud ◽

Sujatha Ramasamy ◽

Khalijah Awang

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Chemical Constituents ◽

Cancer Cell Lines ◽

Phytochemical Study ◽

Triterpene Acid ◽

Human Cancer Cell Lines ◽

Esi Ms

A phytochemical study on the bark of Walsura pinnata has led to the isolation of a new oleanane triterpene acid, 3-oxo-olean-9(11),12-dien-28-oic acid (1), together with nine known compounds (2–10). Their structures were established on the basis of the detailed spectroscopic analysis, including one- and two-dimensional NMR, ESI-MS and HR-ESI-MS techniques. Compounds 2, 3, 5, 6 and 8 were isolated from W. pinnata for the first time. Compounds 3 and 4 showed in vitro growth inhibitory activity against two human cancer cell lines MCF-7 and SK-OV-3 with IC50 values within the range of 8.85 - 18.28 μg/mL. To the best of our knowledge, this is the first report on the cytotoxic activity of compound 3 towards both cancer cell lines.

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Synthesis, Anticancer Activity, and Molecular Modeling of New Halogenated Spiro[pyrrolidine-thiazolo-oxindoles] Derivatives

Applied Sciences ◽

10.3390/app10062170 ◽

2020 ◽

Vol 10 (6) ◽

pp. 2170 ◽

Cited By ~ 3

Author(s):

Mohammad Shahidul Islam ◽

Abdullah Mohammed Al-Majid ◽

Fardous F. El-Senduny ◽

Farid A. Badria ◽

A. F. M. Motiur Rahman ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Single Step ◽

One Pot ◽

Antiproliferative Effects ◽

Physiochemical Parameters ◽

Hct 116 ◽

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A one-pot, single-step, and an atom-economical process towards the synthesis of highly functionalized spirooxindoles analogues was efficiently conducted to produce a satisfactory chemical yields (70–93%) with excellent relative diastereo-, and regio-selectivity. An in vitro antiproliferative assay was carried out on different cancer cell lines to evaluate the biological activity of the synthesized tetrahydro-1’H-spiro[indoline-3,5’-pyrrolo[1,2-c]thiazol]-2-one 5a–n. The prepared hybrids were then tested in vitro for their antiproliferative effects against three cancer cell lines, namely, HepG2 (liver cancer), MCF-7 (breast cancer), and HCT-116 (colon cancer). The spirooxindole analogue 5g exhibited a broad activity against HepG2, MCF-7, and HCT-116 cell lines of liver, breast, and colorectal cancers when compared to cisplatin. Modeling studies including shape similarity, lipophilicity scores, and physicochemical parameters were calculated. The results of this study indicated that spirooxindole analogue 5g retained a good physiochemical parameters with acceptable lipophilicity scores.

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Cytotoxic Constituents of Pachyrhizus Tuberosus from Peruvian Amazon

Natural Product Communications ◽

10.1177/1934578x1300801022 ◽

2013 ◽

Vol 8 (10) ◽

pp. 1934578X1300801 ◽

Cited By ~ 1

Author(s):

Olga Leuner ◽

Jaroslav Havlik ◽

Milos Budesinsky ◽

Vladimir Vrkoslav ◽

Jessica Chu ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Mtt Assay ◽

Human Cancer ◽

Chemical Constituents ◽

Human Fibroblasts ◽

Cancer Cell Lines ◽

Cytotoxic Effects ◽

Hct 116 ◽

Mcf 7

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 μg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 μg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 μg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 μg/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

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Metamizole (dipyrone) – cytotoxic and antiproliferative effects on HeLa, HT-29 and MCF-7 cancer cell lines

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2018.1511382 ◽

2018 ◽

Vol 32 (5) ◽

pp. 1327-1337

Author(s):

Irina Nikolova ◽

Lyubomir Marinov ◽

Ani Georgieva ◽

Reneta Toshkova ◽

Martin Malchev ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Antiproliferative Effects ◽

Ht 29 ◽

Mcf 7

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Study on the chemical constituents of Dacrydium elatum and their cytotoxic activity

Zeitschrift für Naturforschung B ◽

10.1515/znb-2018-0214 ◽

2019 ◽

Vol 74 (2) ◽

pp. 197-201

Author(s):

Tran Thi Phuong Thao ◽

Nguyen Thi Lieu ◽

Pham Thi Ninh ◽

Tran Van Chien ◽

Dinh Thi Phong ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

Cancer Cell Lines ◽

Etoac Extract ◽

Extensive Analysis ◽

Medium Activity ◽

Ft Ir ◽

Mcf 7

AbstractFrom an ethyl acetate extract of twigs and barks of Dacrydium elatum a new diterpenoid named dacrydianone (1), together with lambertic acid (2), three ecdysteroids: 20-hydroxyecdysone (3), ajugasterone C (4), ponasterone A (5), and daucosterol (6), has been isolated. Their structures were elucidated by an extensive analysis of the UV/Vis, FT-IR, MS and NMR spectra as well as comparison with those in published literature. The EtOAc extract and the isolated compounds 1–5 were evaluated for their cytotoxicity on four cancer cell lines: breast cancer (MCF-7), lung cancer (Lu-1), liver cancer (HepG2) and human oral cancer (KB). The EtOAc extract and compound 2 showed a medium activity on four tested cancer cell lines, while compounds 3 and 5 exhibited week activity on these cell lines. Compounds 1 and 4 did not show activity on the tested cell lines.

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Synthesis, Anti-Cancer and Anti-Migratory Evaluation of 3,6-Dibromocarbazole and 5-Bromoindole Derivatives

Molecules ◽

10.3390/molecules24152686 ◽

2019 ◽

Vol 24 (15) ◽

pp. 2686 ◽

Cited By ~ 1

Author(s):

Krystal M. Butler-Fernández ◽

Zulma Ramos ◽

Adela M. Francis-Malavé ◽

Joseph Bloom ◽

Suranganie Dharmawardhane ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Migration Activity ◽

Antiproliferative Effects ◽

Metastatic Cell Line ◽

Anti Cancer ◽

Mcf 7

In this study, a new series of N-alkyl-3,6-dibromocarbazole and N-alkyl-5-bromoindole derivatives have been synthesized and evaluated in vitro as anti-cancer and anti-migration agents. Cytotoxic and anti-migratory effects of these compounds were evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines and an insight on the structure-activity relationship was developed. Preliminary investigations of their anti-cancer activity demonstrated that several compounds have moderate antiproliferative effects on cancer cell lines with GI50 values in the range of 4.7–32.2 µM. Moreover, carbazole derivatives 10, 14, 15, 23, and 24 inhibit migration activity of metastatic cell line MDA-MB-231 in the range of 18–20%. The effect of compounds 10, 14, and 15 in extension of invadopodia and filopodia was evaluated by fluorescence microscopy and results demonstrated a reduction in actin-based cell extensions by compounds 10 and 15.

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Bis-sesquiterpene from the Marine Sponge Dysidea fragilis

Natural Product Communications ◽

10.1177/1934578x1601100403 ◽

2016 ◽

Vol 11 (4) ◽

pp. 1934578X1601100

Author(s):

Phan Van Kiem ◽

Nguyen Xuan Nhiem ◽

Bui Huu Tai ◽

Hoang Le Tuan Anh ◽

Dan Thi Thuy Hang ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Marine Sponge ◽

Human Cancer ◽

2D Nmr ◽

Cancer Cell Lines ◽

Human Cancer Cell ◽

Human Cancer Cell Lines ◽

Esi Ms ◽

Mcf 7

Two new sesquiterpenes and one new bis-sesquiterpene, named dysinidins C-E (1–3) along with three known sterols, dysideasterol F, 9α,11α-epoxycholest-7-en-3β,5α,6α-triol, and 9α,11α-epoxycholest-7-en-3β,5α,6α,19-tetrol 6-acetate (4–6) were isolated from the Vietnamese marine sponge Dysidea fragilis (Montagu, 1814). Their structures were determined by 1D- and 2D-NMR spectroscopies and HR-ESI-MS, as well as by comparison with reported literature data. Compounds 4–6 were found to inhibit eight human cancer cell lines (KB, LU-1, HL-60, LNCaP, SK-Mel-2, HepG-2, MCF-7, and PC-3), with IC50 values ranging from 7.3 to 31.5 μM.

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Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

Functional Foods in Health and Disease ◽

10.31989/ffhd.v8i3.408 ◽

2018 ◽

Vol 8 (3) ◽

pp. 159 ◽

Cited By ~ 1

Author(s):

Meghan Fragis ◽

Abdulmonem I. Murayyan ◽

Suresh Neethirajan

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Cytotoxic Activities ◽

Cancer Line ◽

Mcf 7

Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

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