scholarly journals BMSCs Seeding in Different Scaffold Incorporation with Hyperbaric Oxygen Treat Seawater Immersed Bony Defect

2020 ◽  
Author(s):  
gan zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Xiuwu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Bony Defect ◽  
New Bone Formation ◽  
X Ray ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction: The experiment was undertaken to estimate the effect of BMSCs seeding in different scaffold incorporation with HBO on the repair of seawater immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as scaffold in treatment effect of seawater immersed bone defect.Methods: 60 New Zealand White rabbits with standard seawater defect in radius were randomly divided to group A (implant with nothing), group B (implanted with atuogenous bone), group C (implanted with n-HA/PLGA/BMSCs, and Group D ( implanted with β-TCP/PLGA/BMSCs). After implant, each rabbit receive HBO treatment at 2.4 ATA 100% oxygen for 120 minutes per day for 2 weeeks. Radiograph, histological and biomechanical examination were used to analyze osteogenesis.Result: X-ray analysis show that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was lager than in group D or group A, and close to group B (P<0.05). After 12 weeks, in group A, defect without scaffold show a loose connect tissue filled in the areas. The medullary canal in group B was recanalizated. Defect in group C and D show a larger number of wove bone formation. The new wove bone formation in defect areas in group C was lager than D. The mechanical examination revealed ultimate strength at 12 weeks were group D>group C>group B>group A(P<0.05).Conclusion: Scaffold of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

scholarly journals BMSCs Seeding in Different Scaffold Incorporation with Hyperbaric Oxygen Treat Seawater Immersed Bony Defect

2021 ◽  
Author(s):  
gan zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Xiuwu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Bony Defect ◽  
New Bone Formation ◽  
X Ray ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction: The experiment was undertaken to estimate the effect of BMSCs seeding in different scaffold incorporation with HBO on the repair of seawater immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as scaffold in treatment effect of seawater immersed bone defect.Methods: 60 New Zealand White rabbits with standard seawater defect in radius were randomly divided to group A (implant with nothing), group B (implanted with atuogenous bone), group C (implanted with n-HA/PLGA/BMSCs, and Group D ( implanted with β-TCP/PLGA/BMSCs). After implant, each rabbit receive HBO treatment at 2.4 ATA 100% oxygen for 120 minutes per day for 2 weeeks. Radiograph, histological and biomechanical examination were used to analyze osteogenesis.Result: X-ray analysis show that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was lager than in group D or group A, and close to group B (P<0.05). After 12 weeks, in group A, defect without scaffold show a loose connect tissue filled in the areas. The medullary canal in group B was recanalizated. Defect in group C and D show a larger number of wove bone formation. The new wove bone formation in defect areas in group C was lager than D. The mechanical examination revealed ultimate strength at 12 weeks were group D>group C>group B>group A(P<0.05).Conclusion: Scaffold of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

scholarly journals BMSCs Seeding in Different Scaffold Incorporation with Hyperbaric Oxygen Treat Seawater Immersed Bony Defect

2020 ◽  
Author(s):  
Gan Zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Xiuwu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Bony Defect ◽  
New Bone Formation ◽  
X Ray ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction: The experiment was undertaken to estimate the effect of BMSCs seeding in different scaffold incorporation with HBO on the repair of seawater immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as scaffold in treatment effect of seawater immersed bone defect.Methods: 60 New Zealand White rabbits with standard seawater defect in radius were randomly divided to group A (implant with nothing), group B (implanted with atuogenous bone), group C (implanted with n-HA/PLGA/BMSCs, and Group D ( implanted with β-TCP/PLGA/BMSCs). After implant, each rabbit receive HBO treatment at 2.4 ATA 100% oxygen for 120 minutes per day for 2 weeeks. Radiograph, histological and biomechanical examination were used to analyze osteogenesis.Result: X-ray analysis show that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was lager than in group D or group A, and close to group B (P < 0.05). After 12 weeks, in group A, defect without scaffold show a loose connect tissue filled in the areas. The medullary canal in group B was recanalizated. Defect in group C and D show a larger number of wove bone formation. The new wove bone formation in defect areas in group C was lager than D. The mechanical examination revealed ultimate strength at 12 weeks were group D > group C > group B > group A(P < 0.05).Conclusion: Scaffold of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

scholarly journals BMSC seeding in different scaffold incorporation with hyperbaric oxygen treats seawater-immersed bony defect

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Gan Zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Wuxiu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Autogenous Bone ◽  
Bony Defect ◽  
New Bone Formation ◽  
Woven Bone ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction The experiment was undertaken to estimate the effect of BMSC seeding in different scaffold incorporation with HBO on the repair of a seawater-immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as a scaffold in treatment effect of the seawater-immersed bone defect. Methods Sixty New Zealand White rabbits with standard seawater defect in radius were randomly divided into group A (implant with nothing), group B (implanted with autogenous bone), group C (implanted with n-HA/PLGA/BMSCs), and group D (implanted with β-TCP/PLGA/BMSCs). After the implant, each rabbit receives HBO treatment at 2.4 ATA 100% oxygen for 120 min/day for 2 weeks. Radiograph, histological, and biomechanical examinations were used to analyze osteogenesis. Result X-ray analysis shows that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was larger than that in group D or group A and close to group B (P < 0.05). After 12 weeks, in group A, the defect without scaffold shows a loose connect tissue filled in the areas. The medullary canal in group B was recanalized. Defects in groups C and D show a larger number of woven bone formation. The new woven bone formation in defect areas in group C was larger than that in group D. The mechanical examination revealed ultimate strength at 12 weeks was group D > group C > group B > group A (P < 0.05). Conclusion Scaffolds of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater-immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

Repairing Rabbit Radius Bone Defects with Simvastatin Compound Biological Bone

2021 ◽  
Vol 11 (7) ◽  
pp. 1263-1270
Author(s):  
Zhong-Yu Liu ◽  
Jin-Li Zhang ◽  
Yang Zhang ◽  
Shi-Lian Kan ◽  
Jun Liang ◽  
...  
Keyword(s):  
Bone Defect ◽  
Maximum Stress ◽  
Biomechanical Testing ◽  
Bone Defects ◽  
Bending Test ◽  
Bone Material ◽  
X Ray ◽  
Group A ◽  
Group B ◽  
Group D

Objective: This study aimed to investigate the feasibility of repairing rabbit radius bone defects with simvastatin compound biological bone. Methods: Simvastatin biological bone material was prepared, and osteoblasts were cultured. A total of 42 New Zealand white rabbits were randomly divided into four groups, and a bone defect with a length of 15 mm was created at the middle part of the radial shaft of both limbs in each rabbit, thereby establishing a bone defect model. The grafts in group A were biological bones of osteoblasts combined with simvastatin; the grafts in group B were biological bones of simvastatin; the grafts in group C were biological compound bones of osteoblasts; and the grafts in group D were simple biological bones. In each group, four animals were randomly sacrificed at the sixth and twelfth week after surgery, and specimens were collected for gross observation, X-ray examination, histological observation, and biomechanical testing. In each group, two animals were randomly sacrificed at the twelfth week after surgery; a three-point bending test was performed using a biomechanical testing machine, and the results were compared with those of a normal radius. Results: The X-ray and histological examinations at 6 and 12 weeks after surgery revealed that the osteogenesis ability of the simvastatin biological bone and osteoblast-simvastatin biological bone was better than that of the osteoblast biological bone and simple biological bone, which was superior in group A and group B to group C and group D. The results of the biomechanical examination revealed that the maximum stress of the normal radius was significantly higher than that of the experimental groups. Among the experimental groups, the difference between group A and group B was not statistically significant, and the maximum stress was higher in groups A and B than in groups C and D. Conclusion: Simvastatin biological bone material can promote the repair of rabbit radius defects and increase the quality of bone healing.

scholarly journals Effect of Low-Pressurized Perfusion with Different Concentration of Elastase on the Aneurysm Formation Rate in the Abdominal Aortic Aneurysm Model in Rabbits

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Maoxiao Nie ◽  
Yunfeng Yan ◽  
Xinhe Li ◽  
Tingting Feng ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Abdominal Aorta ◽  
Formation Rate ◽  
Aneurysm Formation ◽  
Abdominal Aortic ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Establishing an animal model of abdominal aortic aneurysm (AAA) is the key to study the pathogenesis and the pathophysiological features of AAAs. We investigated the effects of low-pressurized perfusion with different concentrations of elastase on aneurysm formation rate in the AAA model. Fifty male New Zealand white rabbits were randomly divided into A, B, C, D, and E groups. 10 μL of normal saline was perfused into the abdominal aorta in group A and 1 U/mL, 10 U/mL, 100 U/mL, or 200 U/mL of elastase was, respectively, perfused for the other four groups. All the animals were perfused for 7 min. Doppler ultrasound examinations of the abdominal aorta were performed before surgery and on day 14 after surgery. The rabbits were sacrificed and the perfused segment of the abdominal aorta was observed visually and after staining. The aneurysm formation rate of group A, group B, group C, group D, and group E was, respectively, 0%, 0%, 33.3%, 102.5–146.8%, and 241.5–255.2%. The survival rate of five groups was 90%, 90%, 90%, 90%, and 40%, respectively. So, we concluded that low-pressurized perfusion with 100 U/mL of elastase can effectively establish AAAs in rabbits with a high aneurysm formation rate.

scholarly journals Results of the Study of Bone Tissue Density in Patients of the Study Groups at the Restoration of Bone Defects by Various Osteoplastic Materials

2021 ◽  
Vol 6 (2) ◽  
pp. 158-162
Author(s):  
A. V. Bambuliak ◽  
◽  
N. B. Kuzniak ◽  
R. R. Dmitrenko ◽  
S. V. Tkachik ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Density ◽  
Bone Tissue ◽  
Bone Defect ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
X Ray ◽  
Group A ◽  
X Ray Computed ◽  
Group B

The restoration processes of damaged or lost bone tissue are an important and topical issue of surgical dentistry and maxillofacial surgery today. According to statistics, diseases that are accompaning by destructive changes in bone tissue occupy one of the dominant places among the nosologies of MFA. Along with the use of updated methods of surgical technique, both before and now the problem of choice of bone-plastic material is relevant, because it is known that osteoplasty opens up great opportunities for complete rehabilitation of patients. The purpose of the study was to determine the effectiveness of our developed osteoplastic composition for the restoration of bone defects in dentistry. Materials and methods. X-ray computed tomography to determine bone density was performed in 26 patients with bone defect replacement "Kolapan-L" (group A), 28 people during augmentation of our proposed osteoplastic composition "Kolapan-L" + multipotent mesenchymal stromal cells + platelet-rich plasma) (group B), and 25 patients where the healing of the bone defect occurred spontaneously (group B). X-ray computed tomography was performed on a 16-slice spiral computed tomography Siemens Somatom Emotionc. Image processing was performed using the program "Dicom". Statistical processing of research results was carried out using conventional methods of variation statistics. Results and discussion. After 1 year of research, the average value of the bone density in patients of group B was probably higher: 1.5 times and 1.8 times relative to the corresponding data in groups A and B. At the same time, the minimum value of the bone density in patients of subgroups A and B was 1.5 times and 1.8 times lower than in persons of group B (p <0.01, p1 <0.01). At the same time, the maximum values of the bone density in patients of group B, where the augmentation of bone defects was performed using our proposed composition, were 1.4 times (p <0.01) and 1.8 times (p <0.05, p1 <0.01) higher than in group A, in the replacement of bone defects "Kolapan-L" and in group B, where the healing of the bone defect was spontaneous, respectively. It was found that in patients of group B after 12 months of studies, the average density of osteoregeneration was 1036.69±55.53 (HU), which was 1.5 times and 1.8 times more than in group A (p <0.01) and in group B patients (p <0.05, p1 <0.01) respectively. Conclusion. The use of tissue equivalent of bone tissue, proposed by us to replace a bone defect based on multipotent mesenchymal adipose tissue cells, contributed to the maximum increase in bone density, with a slightly lower effect of increasing bone density in the augmentation of bone defects

Simple Curettage without Bone Grafting for Enchondromata of the Hand: With Special Reference to Replacement of the Cortical Window

2002 ◽  
Vol 27 (5) ◽  
pp. 446-451 ◽  
Author(s):  
T. GOTO ◽  
S. YOKOKURA ◽  
H. KAWANO ◽  
A. YAMAMOTO ◽  
K. MATSUDA ◽  
...  
Keyword(s):  
Local Recurrence ◽  
Bone Formation ◽  
Mechanical Strength ◽  
Special Reference ◽  
Bone Grafting ◽  
Functional Results ◽  
New Bone Formation ◽  
Group A ◽  
Group B

We studied 23 patients (25 bones) with enchondromata of the hand which were treated with simple curettage without bone grafting. The cortical window was replaced in 18 bones (group A), whereas it was not replaced in six bones (group B). In one bone, only half of the cortical window was replaced. Local recurrence was not seen in any patient. Although bone grafting was not performed, new bone formation was observed in all the patients. Radiographic and functional results were excellent in most bones. Restoration of the continuity of the cortex was seen at 3 (range, 1.5–4) months in group A and 8 (range, 6–12) month s in group B. This restoration is important for the recovery of mechanical strength and we therefore consider that the cortical window should be replaced, unless this is impractical.

Cervical motion preservation using mesenchymal progenitor cells and pentosan polysulfate, a novel chondrogenic agent: preliminary study in an ovine model

2010 ◽  
Vol 28 (6) ◽  
pp. E4 ◽  
Author(s):  
Tony Goldschlager ◽  
Peter Ghosh ◽  
Andrew Zannettino ◽  
Stan Gronthos ◽  
Jeffrey V. Rosenfeld ◽  
...  
Keyword(s):  
Bone Formation ◽  
Progenitor Cells ◽  
Control Group ◽  
Cartilaginous Tissue ◽  
Mesenchymal Progenitor Cells ◽  
Pentosan Polysulfate ◽  
New Bone Formation ◽  
Significant Difference ◽  
Group A ◽  
Group B

Object There is an unmet need for a procedure that could generate a biological disc substitute while at the same time preserving the normal surgical practice of achieving anterior cervical decompression. The objective of the present study was to test the hypothesis that adult allogeneic mesenchymal progenitor cells (MPCs) formulated with a chondrogenic agent could synthesize a cartilaginous matrix when implanted into a biodegradable carrier and cage, and over time, might serve as a dynamic interbody spacer following anterior cervical discectomy (ACD). Methods Eighteen ewes were divided randomly into 3 groups of 6 animals. Each animal was subjected to C3–4 and C4–5 ACD followed by implantation of bioresorbable interbody cages and graft containment plates. The cage was packed with 1 of 3 implants. In Group A, the implant was Gelfoam sponge only. In Group B, the implant consisted of Gelfoam sponge with 1 million MPCs only. In Group C, the implant was Gelfoam sponge with 1 million MPCs formulated with the chondrogenic agent pentosan polysulfate (PPS). In each animal the cartilaginous endplates were retained intact at 1 level, and perforated in a standardized manner at the other level. Allogeneic ovine MPCs were derived from a single batch of immunoselected and culture-expanded MPCs isolated from bone marrow of outbred sheep (mixed stock). Radiological and histological measures were used to assess cartilage formation and the presence or absence of new bone formation. Results The MPCs with or without PPS were safe and well-tolerated in the ovine cervical spine. There was no significant difference between groups in the radiographic or histological outcome measures, regardless of whether endplates were perforated or retained intact. According to CT scans obtained at 3 months after the operation, new bone formation within the interbody space was observed in the Gelfoam only group (Group A) in 9 (75%) of 12 interbody spaces, and 11 (92%) of 12 animals in the MPC cohort (Group B) had new bone formation within the interbody space. Significantly, in the MPC & PPS group (Group C), there were only 1 (8%) of 12 levels with new bone formation (p = 0.0009 vs Group A; p = 0.0001 vs Group B). According to histological results, there was significantly more cartilaginous tissue within the interbody cages of Group C (MPC & PPS) compared with both the control group (Group A; p = 0.003) and the MPC Group (p = 0.017). Conclusions This study demonstrated the feasibility of using MPCs in combination with PPS to produce cartilaginous tissue to replace the intervertebral disc following ACD. This biological approach may offer a means preserving spinal motion and offers an alternative to fusion to artificial prostheses.

scholarly journals A Study of the Relationship between the Polymorphism and Mutation of rs682429 and rs3781590 in the LRP5 Gene and Bone Metabolism in Postmenopausal Type 2 Diabetic Women in Xinjiang

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Jun Li ◽  
SiYuan Li ◽  
HuiRong Zhao ◽  
JiaJia Li ◽  
Shuang Wang ◽  
...  
Keyword(s):  
Bone Mass ◽  
Bone Metabolism ◽  
Postmenopausal Women ◽  
X Ray ◽  
Group A ◽  
Mass Group ◽  
Group B ◽  
The Relationship ◽  
Group D

Objective. To explore the expression of the polymorphism and mutation of rs682429 and rs3781590 in the low-density lipoprotein receptor-related protein 5 (LRP5) genotype and to analyse the relationship of bone mineral density (BMD) and bone metabolism markers in postmenopausal women with type-2 diabetes mellitus (T2DM) in Xinjiang, China, to provide a basis for prevention and treatment of the disease. Methods. A total of 136 postmenopausal women were included in the study. According to the results of an oral glucose tolerance test (OGTT) and dual-energy X-ray (DEXA) determination of BMD, the study subjects were divided into 4 groups: group A: normal OGTT+normal bone mass group; group B: normal OGTT+osteoporotic (OP) group; group C: T2DM+normal bone mass group; group D: T2DM+osteoporotic (OP) group. Calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), and clinical biochemical data were determined; haemoglobin A1c (HbA1c) was measured by HPLC; BMD of the femoral neck, hip, and lumbar spine (L1-4) was measured by dual-energy X-ray (DEXA); and the rs682429 and rs3781590 polymorphisms of the LRP5 gene were detected by time-of-flight mass spectrometry (TOF MS). Results. (1) The rs682429 polymorphism of the LRP5 genotype distribution was statistically significant (P<0.05) in group B compared with group A. (2) The triglycerides (TG) of women with the CT/TT genotype (mutant type) were higher than those of women with the CC genotype (wild type) (2.37±1.30 vs. 1.52±0.83, P<0.05) at the rs3781590 site of the LRP5 gene in group D. (3) Multiple linear regression analysis showed that TG (β=0.034, P<0.05) and body mass index (BMI) (β=0.013, P<0.05) were the influencing factors of BMD (L1-4) in T2DM patients. TG (β=0.022, P<0.05), BMI (β=0.009, P<0.05), and duration of menopause (β=0.005, P<0.05) were the influencing factors of BMD (hip). Conclusion. (1) The rs682429 polymorphism site in the LRP5 gene may be involved in bone metabolism in postmenopausal women from Xinjiang. (2) The rs3781590 mutation in the LRP5 gene from these subjects may be involved in lipid metabolism. (3) Among postmenopausal women with type 2 diabetes mellitus and bone mass abnormality in the Xinjiang Shihezi area, high BMI and TG are protective factors against increased BMD. Duration of menopause is a risk factor for increased BMD.

Effect of water glass coating of tricalcium phosphate granules on in vivo bone formation

2018 ◽  
Vol 33 (5) ◽  
pp. 662-672
Author(s):  
Seung Min Ryu ◽  
Myun Whan Ahn ◽  
Chul Hyun Park ◽  
Gun Woo Lee ◽  
In Hwan Song ◽  
...  
Keyword(s):  
Bone Formation ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Water Glass ◽  
Cylindrical Tube ◽  
Control Group ◽  
New Bone Formation ◽  
Group A ◽  
Group B

Background Recently, some authors introduced a water glass (WG, sodium-silicate glass; Na2O·SiO2·nH2O) coating over tricalcium phosphate (TCP) bioceramic to modulate its resorption rate and enhance the bone cell behaviors. In this study, four different types of granular samples were prepared to evaluate the ability of new bone formation in vivo using micro-computed tomography and histology. Methods Four types sample groups: group A (pure HA as a negative resorption control); group B (pure TCP as a positive resorption control); group C (WG-coated TCP as an early resorption model); and group D (same as group C but heat-treated at 500°C as a delayed resorption model). Cylindrical tube-type carriers with holes were fabricated with HA by extrusion and sintering. Each carrier was filled densely with each granular sample. Four types of tubes were implanted into the medial femoral condyle and medial tibial condyle of New Zealand White rabbits. Results The HA group (A) showed the lowest amount of new bone formation. All the TCP sample groups (B, C, and D) showed more new bone formation. On the other hand, among the TCP groups, group C (early resorption model) showed slightly more bone formation. The amount of residual bioceramics was most abundant in the HA group (A). All the TCP sample groups showed less residual bioceramics than group A. Among the TCP groups, group C showed slightly more residual bioceramics. Group B showed the lowest amount of residual bioceramics. Conclusions The WG-coated TCP sample (group C) is the best bone substitute candidate because of its proper biodegradation rate and the Si ions release because the WG-coated layer reduces the material resorption and enhances the new bone formation. That is, the WG-coated TCP is believed to be the best material for the application of an artificial bone substitute material.

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