Presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

Abstract Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 119 CoViD-19 patients. The high-throughput assay enables to follow the specific immune response of ultimate 384 individuals for these four parameters in one run. The measured IgG, IgM and IgA levels correlated with ELISA (Euroimmun: Anti-SARS-CoV-2, IgG assay, r-0.95, ECLIA: Anti-SARS-CoV-2 Ig electrochemiluminescence r=0.73). During the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels went down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immune globulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 and can be applied for the assessment of the immune reaction of healthy individuals in vaccination programmes.

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High throughput Surface Plasmon Resonance imaging method for clinical detection of presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

MethodsX ◽

10.1016/j.mex.2021.101432 ◽

2021 ◽

pp. 101432

Author(s):

Richard B.M. Schasfoort ◽

Jos van Weperen ◽

Margot van Amsterdam ◽

Judicaël Parisot ◽

Jan Hendriks ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

High Throughput ◽

Plasmon Resonance ◽

Imaging Method ◽

Surface Plasmon Resonance Imaging ◽

Resonance Imaging ◽

Iga Antibodies ◽

Clinical Detection

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Benzo[a]pyrene Sensing Properties of Surface Plasmon Resonance Imaging Sensor Based on the Hue Algorithm

Acta Physico-Chimica Sinica ◽

10.3866/pku.whxb201701131 ◽

2017 ◽

Vol 33 (5) ◽

pp. 1001-1009

Author(s):

Zhi-Bo FAN ◽

◽

Xiao-Qing GONG ◽

Dan-Feng LU ◽

Ran GAO ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Surface Plasmon Resonance Imaging ◽

Resonance Imaging ◽

Sensing Properties ◽

Imaging Sensor

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Stereotypic neutralizing VH antibodies against SARS-CoV-2 spike protein receptor binding domain in COVID-19 patients and healthy individuals

Science Translational Medicine ◽

10.1126/scitranslmed.abd6990 ◽

2021 ◽

pp. eabd6990

Author(s):

Sang Il Kim ◽

Jinsung Noh ◽

Sujeong Kim ◽

Younggeun Choi ◽

Duck Kyun Yoo ◽

...

Keyword(s):

B Cells ◽

Receptor Binding ◽

Neutralizing Antibodies ◽

De Novo ◽

Binding Domain ◽

Host Cells ◽

Spike Protein ◽

Receptor Binding Domain ◽

Healthy Individuals

Stereotypic antibody clonotypes exist in healthy individuals and may provide protective immunity against viral infections by neutralization. We observed that 13 out of 17 patients with COVID-19 had stereotypic variable heavy chain (VH) antibody clonotypes directed against the receptor-binding domain (RBD) of SARS-CoV-2 spike protein. These antibody clonotypes were comprised of immunoglobulin heavy variable (IGHV)3-53 or IGHV3-66 and immunoglobulin heavy joining (IGHJ)6 genes. These clonotypes included IgM, IgG3, IgG1, IgA1, IgG2, and IgA2 subtypes and had minimal somatic mutations, which suggested swift class switching after SARS-CoV-2 infection. The different immunoglobulin heavy variable chains were paired with diverse light chains resulting in binding to the RBD of SARS-CoV-2 spike protein. Human antibodies specific for the RBD can neutralize SARS-CoV-2 by inhibiting entry into host cells. We observed that one of these stereotypic neutralizing antibodies could inhibit viral replication in vitro using a clinical isolate of SARS-CoV-2. We also found that these VH clonotypes existed in six out of 10 healthy individuals, with IgM isotypes predominating. These findings suggest that stereotypic clonotypes can develop de novo from naïve B cells and not from memory B cells established from prior exposure to similar viruses. The expeditious and stereotypic expansion of these clonotypes may have occurred in patients infected with SARS-CoV-2 because they were already present.

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Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

Scientific Reports ◽

10.1038/s41598-021-83019-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ariel Munitz ◽

L. Edry-Botzer ◽

M. Itan ◽

R. Tur-Kaspa ◽

D. Dicker ◽

...

Keyword(s):

Nucleocapsid Protein ◽

Neutralizing Antibodies ◽

Host Response ◽

Humoral Response ◽

Rapid Onset ◽

Response Analysis ◽

Receptor Binding Domain ◽

Igg Antibodies ◽

Viral Receptor ◽

Iga Antibodies

AbstractDespite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.

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