Zinc Oxide Nanoparticles Ameliorates Dimethylnitrosamine Induced Renal Toxicity in Rat

Abstract Dimethylnitrosamine (DMN) is an established carcinogen. It is toxic to several organs viz. liver, kidney, lungs and immune system. Several drugs have been used in the past to modulate its toxicity using experimental models. Present study was designed to investigate the effect of zinc oxide nanoparticles (ZnONPs) on renal toxicity caused by DMN in laboratory rat. Since oxidative mechanisms form etiological causing its toxicity, the proposed study focuses on amelioration of oxidative stress expressed by ZnONPs, if any. Present results show that administration of ZnONPs (50 mg/kg) to DMN (2μl/100g body weight) treated rats diminuted the concentration of malonaldehyde, H2O2 and NO in the kidney. However, GSH concentration increased after ZnONPs treatment. Results on glutathione S-transferase, glutathione peroxidase favored an antioxidative effect of ZnONPs. These results find support from diminished oxidative DNA damage manifested by ZnONPs in DMN treated rats. Histopathological results on kidney also indicate a protective effect of ZnONPs on renal toxicity of DMN. A comparison of results between control and ZnONPs treated rats suggests that ZnONPs might be toxic to renal tissue. Nevertheless, the study suggests that ZnONPs possess strong therapeutic/antioxidative potential against DMN induced renal toxicity.

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Zinc Oxide Nanoparticles Ameliorate Dimethylnitrosamine-Induced Renal Toxicity in Rat

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-021-03689-4 ◽

2021 ◽

Author(s):

Varsha Rani ◽

Yeshvandra Verma ◽

S. V. S. Rana

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Renal Toxicity ◽

Oxide Nanoparticles

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The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro

Materials ◽

10.3390/ma12244062 ◽

2019 ◽

Vol 12 (24) ◽

pp. 4062

Author(s):

Till Jasper Meyer ◽

Agmal Scherzad ◽

Helena Moratin ◽

Thomas Eckert Gehrke ◽

Julian Killisperger ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Oxidative Dna Damage ◽

Clonogenic Survival ◽

Oxide Nanoparticles ◽

Radiosensitizing Effect ◽

Primary Fibroblasts

Radioresistance is an important cause of head and neck cancer therapy failure. Zinc oxide nanoparticles (ZnO-NP) mediate tumor-selective toxic effects. The aim of this study was to evaluate the potential for radiosensitization of ZnO-NP. The dose-dependent cytotoxicity of ZnO-NP20 nm and ZnO-NP100 nm was investigated in FaDu and primary fibroblasts (FB) by an MTT assay. The clonogenic survival assay was used to evaluate the effects of ZnO-NP alone and in combination with irradiation on FB and FaDu. A formamidopyrimidine-DNA glycosylase (FPG)-modified single-cell microgel electrophoresis (comet) assay was applied to detect oxidative DNA damage in FB as a function of ZnO-NP and irradiation exposure. A significantly increased cytotoxicity after FaDu exposure to ZnO-NP20 nm or ZnO-NP100 nm was observed in a concentration of 10 µg/mL or 1 µg/mL respectively in 30 µg/mL of ZnO-NP20 nm or 20 µg/mL of ZnO-NP100 nm in FB. The addition of 1, 5, or 10 µg/mL ZnO-NP20 nm or ZnO-NP100 nm significantly reduced the clonogenic survival of FaDu after irradiation. The sub-cytotoxic dosage of ZnO-NP100 nm increased the oxidative DNA damage compared to the irradiated control. This effect was not significant for ZnO-NP20 nm. ZnO-NP showed radiosensitizing properties in the sub-cytotoxic dosage. At least for the ZnO-NP100 nm, an increased level of oxidative stress is a possible mechanism of the radiosensitizing effect.

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Zinc oxide nanoparticles induce oxidative DNA damage and ROS-triggered mitochondria-mediated apoptosis in zebrafish embryos

Aquatic Toxicology ◽

10.1016/j.aquatox.2016.09.013 ◽

2016 ◽

Vol 180 ◽

pp. 56-70 ◽

Cited By ~ 88

Author(s):

Xuesong Zhao ◽

Xin Ren ◽

Rong Zhu ◽

Zhouying Luo ◽

Baixiang Ren

Keyword(s):

Dna Damage ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Oxidative Dna Damage ◽

Oxide Nanoparticles ◽

Zebrafish Embryos

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Zinc oxide nanoparticles induce renal toxicity through reactive oxygen species

Food and Chemical Toxicology ◽

10.1016/j.fct.2016.02.002 ◽

2016 ◽

Vol 90 ◽

pp. 76-83 ◽

Cited By ~ 28

Author(s):

Lu Xiao ◽

Chunhua Liu ◽

Xiaoniao Chen ◽

Zhuo Yang

Keyword(s):

Reactive Oxygen Species ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Renal Toxicity ◽

Reactive Oxygen ◽

Oxide Nanoparticles ◽

Oxygen Species

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Toxicological perturbations of zinc oxide nanoparticles in the Coelatura aegyptiaca mussel

Toxicology and Industrial Health ◽

10.1177/0748233716687927 ◽

2017 ◽

Vol 33 (7) ◽

pp. 564-575 ◽

Cited By ~ 9

Author(s):

Sohair R Fahmy ◽

Dawlat A Sayed

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Reduced Glutathione ◽

Aquatic Organisms ◽

Consumer Products ◽

Oxide Nanoparticles ◽

Glutathione S Transferase ◽

Stress Induction ◽

Glandular Cells ◽

Gill Histopathology

More research is needed to understand the interactions of nanoparticles (NPs) with aquatic organisms and their mechanism of toxic action. Zinc oxide nanoparticles (ZnONPs) are the most used engineered metal oxide NPs in consumer products. The present study was designed to evaluate the cytotoxicity, genotoxicity and digestive gland (DG) as well as gill histopathology of the freshwater molluscan bivalve Coelatura aegyptiaca following exposure to ZnONPs (2, 10 and 50 mg/L) for 6 consecutive days. Exposure to ZnONPs (10 and 50 mg/L) induced a significant increase in malondialdehyde, superoxide dismutase and nitric oxide with a concomitant decrease in reduced glutathione, glutathione-S-transferase and catalase levels in the haemolymph, DG and gills of the treated mussels. Following exposure to ZnONPs (50 mg/L), the DG exhibited gradual changes in glandular activity showing hypertrophy and hyperplasia in the glandular cells and irregularity of lamellae and swelling of filaments in the gills. The present investigation revealed that oxidative stress induction, genotoxicity in the haemocytes and histological alterations in the DG and gills of C. aegyptiaca could be the main mechanisms involved in ZnONPs toxicity in aquatic organisms. Thereby, it is suggested that ZnONPs should be applied with more precautions in relevant industries, and occupational health surveillance should be necessarily considered.

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