scholarly journals Association of significantly elevated serum levels of NGAL and IGFBP4 in patients with diabetic nephropathy

2021 ◽  
Author(s):  
Hamad Ali ◽  
Mohamed Abu-Farha ◽  
Eman Alshawaf ◽  
Sriraman Devarajan ◽  
Yousif Bahbahani ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Significant Positive Correlation ◽  
Elevated Serum ◽  
Serum Levels ◽  
Related Complication ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Patients With Diabetes ◽  
Neutrophil Gelatinase ◽  
And Control ◽  
Igfbp 3

Abstract Background: Diabetic nephropathy (DN) is a kidney-related complication affecting approximately 40% of patients with diabetes. Current DN diagnostic criteria predominantly rely on albuminuria and serum creatinine levels; however, the specificity and reliability of both markers are limited. Hence, reliable biomarkers are required to diagnose and effectively manage DN progression. Methods: Here we investigated the expression level and the association between neutrophil gelatinase-associated lipocalin (NGAL), IGFBP-1, IGFBP-3, and IGFBP-4 in patients with DN and compared it to patients with T2D and control participants. A cohort comprise of 159 individuals (DN = 67) was clinically evaluated and circulatory levels of NGAL, IGFBP1, IGFBP3, and IGFBP4 were determined using ELISA. Results: Levels of circulating NGAL were significantly higher in people with DN compared to people with T2D and non-diabetic groups (92.76 ± 7.5, 57.22 ± 8.7, and 52.47 ± 2.9 mg/L, respectively; p < 0.0001). IGFBP4 showed a similar pattern, where it was highest in people with DN (795.61 ng/ml ±130.7) compared to people with T2D and non-diabetic respectively (374.56 ng/ml ±86.8, 273.06 ng/ml ±27.8, ANOVA p<0.01). Our analysis presents a significant positive correlation between NGAL and IGFBP4 in people with DN (ρ =.620, p <0.005). IGFBP4 also correlated positively with creatinine level and negatively with eGFR, in people with DN supporting its involvement in DN. Conclusion: Our results report the association between the rise in NGAL and IGFBP4 levels in DN and suggest them as potential markers to aid DN diagnosis.

scholarly journals Serum Levels of Neutrophil Gelatinase Associated Lipocalin (NGAL) Predicts Hemodialysis After Coronary Angiography in High Risk Patients with Acute Coronary Syndrome.

2019 ◽  
Author(s):  
Luis Felipe Reyes ◽  
Carlos A. Bustamante ◽  
Diego Fernando Severiche-Bueno ◽  
Sixta Murillo ◽  
Nilam J. Soni ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
High Risk ◽  
Elevated Serum ◽  
Area Under The Curve ◽  
Kidney Injury ◽  
Serum Levels ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Coronary Syndrome ◽  
Contrast Exposure ◽  
Neutrophil Gelatinase

Abstract Aim To determine the association of serum the Neutrophil Gelatinase Associated Lipocalin (NGAL) levels and the need for hemodialysis after percutaneous coronary intervention (PCI).Design This is a prospective, observational study.Setting A cardiovascular and University referral hospital.Patients Patients with Acute Coronary Syndrome (ACS) that underwent PCI, during two consecutive years were enrolled in our study.Main variables of interest NGAL levels were measured using ELISA. Blood samples were obtained within the first 6 hours of hospital admission, and 12 and 24 hours after contrast exposure from angiography. The primary outcome was the requirement of hemodialysis. The non-parametric Mann-Whitney U Test was used to test for differences in median serum levels of NGAL. A receiver operating characteristic (ROC) curve was developed to assess the accuracy of NGAL to predict need for hemodialysis after PCI.Results A total of 2,875 were screened; however, 45 patients with ACS that underwent PCI were included. All patients were at high risk of developing Contrast-induced nephropathy (CIN) defined by Mehran score >11 points. The median (IQR) serum concentration of NGAL was significantly higher in patients that required versus did not require hemodialysis (340 [83-384] vs. 169 [100-210], p=0.01). Elevated serum levels of NGAL predicted the need for hemodialysis with an area under the curve of 0.86 (95% CI: 0.66-1.00).Conclusions In patients with ACS undergoing PCI and high risk of developing CIN, an elevated serum level of NGAL 6 hours after contrast exposure predicts development of acute kidney injury requiring hemodialysis.

scholarly journals Serum Levels of Neutrophil Gelatinase Associated Lipocalin (NGAL) Predicts Hemodialysis After Coronary Angiography in High Risk Patients with Acute Coronary Syndrome.

2020 ◽  
Author(s):  
Luis Felipe Reyes ◽  
Diego Fernando Severiche-Bueno ◽  
Carlos A. Bustamante ◽  
Sixta Murillo ◽  
Nilam J. Soni ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
High Risk ◽  
Elevated Serum ◽  
Kidney Injury ◽  
Serum Levels ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Coronary Syndrome ◽  
Risk Patients ◽  
Contrast Exposure ◽  
Neutrophil Gelatinase

Abstract Background: Contrast-induced nephropathy (CIN) following a percutaneous coronary intervention (PCI) is the third most common cause of acute kidney injury (AKI) worldwide. Patients who require hemodialysis secondary to CIN have an elevated mortality rate as high as 55%. The current definition of CIN is based on an elevation of creatinine and decrease in urinary output. Creatinine typically increases 48 hours after the contrast exposure, which delays the diagnosis and treatment of CIN. The neutrophil gelatinase associated lipocalin (NGAL) has emerged as a sensitive and specific biomarker of renal injury. Limited data exists about the effectiveness of NGAL to predict CIN in high-risk patients with acute coronary syndrome (ACS) that underwent PCI. The primary aim of this study was to determine the association of serum NGAL levels and the need for hemodialysis after PCI. Methods: This is a prospective, observational study. NGAL levels were measured using ELISA. Blood samples were obtained within the first 6 hours of hospital admission, and 12 and 24 hours after contrast exposure from angiography. The primary outcome was the requirement of hemodialysis. The non-parametric Mann-Whitney U test was used to test for differences in median serum levels of NGAL. A receiver operating characteristic (ROC) curve was developed to assess the accuracy of NGAL to predict the need for hemodialysis after PCI. Results: A total of 2,875 were screened; however, 45 patients with ACS that underwent PCI were included. All patients were at high risk of developing CIN defined by Mehran score >11 points. The median (IQR) serum concentration of NGAL was significantly higher in patients that required versus did not require hemodialysis (340 [83-384] vs. 169 [100-210], p=0.01). Elevated serum levels of NGAL with a cut-off at 6 hours post PCI of 281 mg/dL predicted the need for hemodialysis with an area under the curve of 0.86 (95% CI: 0.66-1.00). Conclusions: In patients with ACS undergoing PCI; and high risk of developing CIN, an elevated serum level of NGAL 6 hours after contrast exposure predicts the development of acute kidney injury requiring hemodialysis.

scholarly journals Changes in Urinary and Serum Levels of Novel Biomarkers after Administration of Gadolinium-based Contrast Agents

2016 ◽  
Vol 11 ◽  
pp. BMI.S39199 ◽  
Author(s):  
Habib Mawad ◽  
Louis-Philippe Laurin ◽  
Jean-François Naud ◽  
François A. Leblond ◽  
Nathalie Henley ◽  
...  
Keyword(s):  
Renal Function ◽  
Contrast Agents ◽  
Cystatin C ◽  
Normal Renal Function ◽  
Serum Levels ◽  
Serum Cystatin C ◽  
Baseline Serum ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Novel Biomarkers ◽  
Neutrophil Gelatinase

Objective The aim of our study is to describe the changes in urinary and serum levels of novel biomarkers after gadolinium contrast administration in patients with normal renal function. Methods We measured four biomarkers in 28 volunteers: interleukin-18 (IL-18), N-acetyl-glucosaminidase (NAG), neutrophil gelatinase-associated lipocalin, and cystatin C. Urinary and serum samples were collected at 0, 3, and 24 hours following gadolinium administration. Results Baseline serum creatinine was 57.8 ± 34.5 μmol/L and remained stable. Urinary IL-18 levels increased significantly at three hours (10.7 vs. 7.3 ng/mg creatinine; P < 0.05). Similarly, urinary NAG levels increased significantly at three hours (3.9 vs. 2.2 IU/mg creatinine; P < 0.001). For both these markers, the difference was no longer significant at 24 hours. No statistically significant differences were observed for urinary and serum neutrophil gelatinase-associated lipocalin levels and for serum cystatin C levels. Conclusions Urinary IL-18 and NAG levels increased transiently after administration of gadolinium-based contrast agents in patients with normal renal function.

scholarly journals Association of serum neutrophil gelatinase associated lipocalin (NGAL) levels with preeclampsia

2019 ◽  
Vol 8 (2) ◽  
pp. 513
Author(s):  
M. Dhivya ◽  
Chippy Tess Mathew ◽  
G. Jeyachandran
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Gestational Age ◽  
Correlation Coefficients ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Multiple Organs ◽  
Systemic Disorder ◽  
Neutrophil Gelatinase ◽  
And Control ◽  
Control Study

Background: Preeclampsia is a systemic disorder that affects multiple organs and is characterized by the new onset of hypertension and proteinuria or end-organ dysfunction or both in the second half of pregnancy. NGAL is a 25-KDa protein of the lipocalin family and is considered to be a novel biomarker for ischemic injury. The objective of this study is to compare the levels of serum NGAL in preeclamptic patients and gestational age matched normotensive controls.Methods: The study design is case control study in which pregnant women with preeclampsia (n=40) are selected as cases. Cases were selected from pregnant women attending OG-OPD and IP satisfying the inclusion criteria and not coming under exclusion criteria. 0.5ml of blood was collected in vacutainers and was centrifuged at 3500rpm for 10 minutes. The serum thus separated was aliquoted into smaller plain containers and stored at -20 degree Celsius for analysis. The urine sample was also collected. Controls were also selected from the OP patients.Results: In present study, the serum NGAL ranged from 40-900ng/ml in cases and from 110-795ng/ml in controls. There is no difference in NGAL between cases and control. The correlation coefficients between the NGAL levels and other parameters like maternal age, gestational age, systolic Blood pressure, diastolic Blood pressure, uric acid levels, urine PCR are also not statistically significant.Conclusions: Serum NGAL levels are not significantly elevated in patients with preeclampsia when compared with the normotensive controls and also there is no significant correlation between serum NGAL levels and other assessed parameters.

scholarly journals Serum Levels of Neutrophil Gelatinase-Associated Lipocalin (NGAL) as Predictor of Acute Kidney Injury in Sickle Cell Subjects

2018 ◽  
Vol 7 (4) ◽  
Author(s):  
Adedeji David Atere ◽  
Olumide Faith Ajani ◽  
David Bolaji Akinbo ◽  
Omobolaji Adewumi Adeosun ◽  
Odiaka Mark Anombem
Keyword(s):  
Acute Kidney Injury ◽  
Sickle Cell ◽  
Kidney Injury ◽  
Serum Levels ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Elevated serum levels of interleukin-18 in patients with overt diabetic nephropathy: effects of miglitol

2010 ◽  
Vol 15 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Takashi Uzu ◽  
Hiroki Yokoyama ◽  
Hirofumi Itoh ◽  
Daisuke Koya ◽  
Atsushi Nakagawa ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Elevated Serum ◽  
Serum Levels ◽  
Interleukin 18

scholarly journals Proteases and Protease Inhibitors of Urinary Extracellular Vesicles in Diabetic Nephropathy

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Luca Musante ◽  
Dorota Tataruch ◽  
Dongfeng Gu ◽  
Xinyu Liu ◽  
Carol Forsblom ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Kidney Disease ◽  
Protease Inhibitors ◽  
Extracellular Vesicles ◽  
Diagnostic Features ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Kidney Involvement ◽  
Natural Inhibitor ◽  
End Stage ◽  
Neutrophil Gelatinase

Diabetic nephropathy (DN) is one of the major complications of diabetes mellitus (DM), leads to chronic kidney disease (CKD), and, ultimately, is the main cause for end-stage kidney disease (ESKD). Beyond urinary albumin, no reliable biomarkers are available for accurate early diagnostics. Urinary extracellular vesicles (UEVs) have recently emerged as an interesting source of diagnostic and prognostic disease biomarkers. Here we used a protease and respective protease inhibitor array to profile urines of type 1 diabetes patients at different stages of kidney involvement. Urine samples were divided into groups based on the level of albuminuria and UEVs isolated by hydrostatic dialysis and screened for relative changes of 34 different proteases and 32 protease inhibitors, respectively. Interestingly, myeloblastin and its natural inhibitor elafin showed an increase in the normo- and microalbuminuric groups. Similarly, a characteristic pattern was observed in the array of protease inhibitors, with a marked increase of cystatin B, natural inhibitor of cathepsins L, H, and B as well as of neutrophil gelatinase-associated Lipocalin (NGAL) in the normoalbuminuric group. This study shows for the first time the distinctive alterations in comprehensive protease profiles of UEVs in diabetic nephropathy and uncovers intriguing mechanistic, prognostic, and diagnostic features of kidney damage in diabetes.

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