scholarly journals Lipoma HMGIC fusion partner expression differs in primary tumors and lymph node metastases in malignancy.

2021 ◽  
Author(s):  
Vijay S
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Node Metastases ◽  
Cancer Metastasis ◽  
Breast Tumors ◽  
Breast Cancer Metastasis ◽  
Axillary Lymph ◽  
Node Metastasis ◽  
Node Metastases

Abstract Breast cancer is the most common cancer found in women. 1. Metastasis is the leading cause of mortality among cancer patients. 2. As the number of axillary lymph nodes with metastases grows the prognosis for patients with breast cancer worsens3. We used a published microarray dataset4 to find genes linked to lymph node metastasis, which is an early stage of breast cancer metastasis. When comparing original breast tumors to lymph node metastases from patients diagnosed with breast cancer, we discovered substantial differences in LHFP gene expression. When comparing primary breast tumors to neighboring normal breast tissue, LHFP was shown to be one of the most differentially expressed genes in a separate microarray dataset5. In individuals with breast cancer, LHFP expression was shown to be substantially linked with median overall survival. LHFP may be involved in the mechanisms that lead to the transformation or progression of the original tumor in human breast cancer, as well as lymph node metastasis. Breast cancer, breast cancer metastasis, lymph node metastasis, LHFP, breast cancer systems biology, and breast cancer targeted treatments.

scholarly journals Lipoma HMGIC fusion partner expression differs in primary tumors and lymph node metastases in malignancy.

2021 ◽  
Author(s):  
Willard A. Levy
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Node Metastases ◽  
Cancer Metastasis ◽  
Breast Tumors ◽  
Breast Cancer Metastasis ◽  
Axillary Lymph ◽  
Node Metastasis ◽  
Node Metastases

Abstract Breast cancer is the most common cancer found in women. 1. Metastasis is the leading cause of mortality among cancer patients. 2. As the number of axillary lymph nodes with metastases grows the prognosis for patients with breast cancer worsens3. We used a published microarray dataset4 to find genes linked to lymph node metastasis, which is an early stage of breast cancer metastasis. When comparing original breast tumors to lymph node metastases from patients diagnosed with breast cancer, we discovered substantial differences in LHFP gene expression. When comparing primary breast tumors to neighboring normal breast tissue, LHFP was shown to be one of the most differentially expressed genes in a separate microarray dataset5. In individuals with breast cancer, LHFP expression was shown to be substantially linked with median overall survival. LHFP may be involved in the mechanisms that lead to the transformation or progression of the original tumor in human breast cancer, as well as lymph node metastasis. Breast cancer, breast cancer metastasis, lymph node metastasis, LHFP, breast cancer systems biology, and breast cancer targeted treatments.

scholarly journals Differentiating the lymph node metastasis of breast cancer through dynamic contrast-enhanced magnetic resonance imaging

BJR|Open ◽  
2019 ◽  
Vol 1 (1) ◽  
pp. 20180023
Author(s):  
Xu Dong ◽  
Yu Chunrong ◽  
Hou Hongjun ◽  
Zhang Xuexi
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Node Metastases ◽  
Area Under The Curve ◽  
Histogram Analysis ◽  
Pharmacokinetic Parameters ◽  
Dce Mri ◽  
Node Metastasis ◽  
Node Metastases

Objective: Lymph node metastasis is an important trait of breast cancer, and tumors with different lymph node statuses require various clinical treatments. This study was designed to evaluate the lymph node metastasis of breast cancer through pharmacokinetic and histogram analysis via dynamic contrast-enhanced (DCE) MRI. Methods and materials: A retrospective analysis was conducted to quantitatively evaluate the lymph node statuses of patients with breast cancer. A total of 75 patients, i.e. 34 patients with lymph node metastasis and 41 patients without lymph node metastasis, were involved in this research. Of the patients with lymph node metastases, 19 had sentinel lymph node metastasis, and 15 had axillary lymph node metastasis. MRI was conducted using a 3.0 T imaging device. Segmentation was carried out on the regions of interest (ROIs) in breast tumors under DCE-MRI, and pharmacokinetic and histogram parameters were calculated from the same ROIs. Mann–Whitney U test was performed, and receiver operating characteristic curves for the parameters of the two groups were constructed to determine their diagnostic values. Results: Pharmacokinetic parameters, including Ktrans, Kep, area under the curve of time–concentration, and time to peak, which were derived from the extended Tofts linear model for DCE-MRI, could highlight the tumor areas in the breast and reveal the increased perfusion. Conversely, the pharmacokinetic parameters showed no significant difference between the patients with and without lymph node metastases. By contrast, the parameters from the histogram analysis yielded promising results. The entropy of the ROIs exhibited the best diagnostic ability between patients with and without lymph node metastases (p < 0.01, area under the curve of receiver operating characteristic = 0.765, specificity = 0.706, sensitivity = 0.780). Conclusion: In comparison with the pharmacokinetic parameters, the histogram analysis of the MR images could reveal the differences between patients with and without lymph node metastases. The entropy from the histogram indicated that the diagnostic ability was highly sensitive and specific. Advances in knowledge: This research gave out a promising result on the differentiating lymph node metastases through histogram analysis on tumors in DCE-MR images. Histogram could reveal the tumors heterogenicity between patients with different lymph node status.

Evaluation of one-step nucleic acid (OSNA) molecular assay for intra-operative diagnosis of sentinel lymph node metastasis.

2012 ◽  
Vol 30 (27_suppl) ◽  
pp. 194-194
Author(s):  
Shramana Mitul Banerjee ◽  
Timothy Davidson ◽  
Nikolaus Michalopoulos ◽  
Nuala McDermott ◽  
Soha El Sheikh ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Lymph Node Metastasis ◽  
Predictive Value ◽  
Axillary Lymph Node ◽  
Axillary Lymph ◽  
Node Metastasis ◽  
One Step ◽  
Node Metastases

194 Background: One step nucleic acid (OSNA) is a recently developed molecular diagnostic assay to detect lymph node metastases. Aim of the present study was to assess OSNA for intra-operative detection of sentinel node metastases with use of standard histopathology as the “gold standard.” Moreover, discordant cases were further investigated to assess their impact on patients’ management. Methods: 157 breast cancer patients with clinically and ultrasonographically negative axilla underwent axillary staging with sentinel node (SLN) biopsy. 244 SLNs were evaluated by OSNA and standard histopathology (alternate slices were submitted to OSNA and standard test). The “turn-around time” taken for an intra-operative OSNA result was recorded. Results: Sensitivity and specificity of OSNA were 92.3% and 95.6% respectively with positive predictive value of 80% and negative predictive value of 98.4%. The average time taken for an intra-operative result in this group was 34 minutes. Concordance rate was 95% while 12/244 nodes showed discordant results between OSNA and Histology. The discordant cases were re-evaluated by histopathologists and characteristics of this subgroup analysed. Patient’s management was altered by OSNA alone in 5 patients as they underwent axillary lymph node dissection on the basis of a positive OSNA when histopathology did not show evidence of metastasis. Two patients with nodes negative on OSNA had histopathology results showing metastases requiring delayed axillary clearance. The rest of the discordant nodes belonged to patients who had other nodes biopsied concurrently that were concordant in OSNA and histology. Conclusions: OSNA is a fast, valuable and highly accurate standardised method for the intra-operative evaluation of axillary lymph node metastasis and is part of our routine intra-operative assessment tool for sentinel nodes in breast cancer.

Tyrosine kinase receptor AXL in human breast cancer is expressed differentially in metastasis of the lymph node

2021 ◽  
Author(s):  
Mairead Paul ◽  
Brandon Weston ◽  
Oliwier Morin
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Nodes ◽  
Cancer Metastasis ◽  
Disease Free Survival ◽  
Tyrosine Kinase Receptor ◽  
Axillary Lymph ◽  
Primary Tumors ◽  
Node Metastasis

The most diagnosed malignancy in women is breast cancer1. Metastases in people diagnosed with cancer are the main cause of mortality. 2. Patients with breast cancer are predicted to do worse as the number of metastasized axillary lymph nodes increases3. In order to uncover the genes related with metastases in lymph nodes, the early events of the breast cancer metastasis, we mined the published and multiplexed mRNA quantitation datasets4, 5. When lymph node metastasis was compared to original breast tumors by patients diagnosed with breast cancer, we identified substantial differential expression of AXL encoding. The lower expression of AXL in primary tumors is associated with reduced disease-free survival in patients with breast cancer. AXL may be important in mechanisms that underlie lymph node metastasis.

scholarly journals Fibrillin-1, encoded by FBN1, is differentially expressed in lymph node metastases in human breast cancer.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Metastases ◽  
Cancer Metastasis ◽  
Axillary Lymph ◽  
Significant Differential Expression ◽  
Primary Tumors ◽  
Fibrillin 1 ◽  
Node Metastases

Breast cancer is the most frequently diagnosed cancer in women (1). Metastasis is the major cause of death in patients diagnosed with cancer (2). Prognosis for patients with breast cancer worsens as the number of axillary lymph nodes with metastasis increases (3). We mined published microarray and multiplexed mRNA quantitation datasets (4, 5) to discover genes associated with metastasis to the lymph nodes, an early event in breast cancer metastasis. We found significant differential expression of the gene encoding fibrillin-1, FBN1, when comparing lymph node metastases to primary breast tumors from women diagnosed with breast cancer. In primary tumors, lower expression of FBN1 correlated with worse overall survival in breast cancer patients in the lowest but not highest survival quartile. FBN1 may be of relevance to processes underlying metastasis to the lymph nodes.

scholarly journals AXL receptor tyrosine kinase is differentially expressed in lymph node metastases in human breast cancer.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Metastases ◽  
Cancer Metastasis ◽  
Disease Free Survival ◽  
Axillary Lymph ◽  
Significant Differential Expression ◽  
Primary Tumors ◽  
Node Metastases

Breast cancer is the most frequently diagnosed cancer in women (1). Metastasis is the major cause of death in patients diagnosed with cancer (2). Prognosis for patients with breast cancer worsens as the number of axillary lymph nodes with metastasis increases (3). We mined published microarray and multiplexed mRNA quantitation datasets (4, 5) to discover genes associated with metastasis to the lymph nodes, an early event in breast cancer metastasis. We found significant differential expression of the gene encoding AXL when comparing lymph node metastases to primary breast tumors from women diagnosed with breast cancer. In primary tumors, lower expression of AXL correlated with decreased disease-free survival in breast cancer patients. AXL may be of relevance to processes underlying metastasis to the lymph nodes.

scholarly journals Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis

Oncogenesis ◽  
2021 ◽  
Vol 10 (10) ◽  
Author(s):  
Kun Xu ◽  
Runtian Wang ◽  
Hui Xie ◽  
Longfei Hu ◽  
Cong Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Single Cell ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Transcriptome Profile ◽  
Primary Tumors ◽  
Node Metastasis ◽  
Single Cell Rna Sequencing

AbstractMolecular mechanisms underlying breast cancer lymph node metastasis remain unclear. Using single-cell sequencing, we investigated the transcriptome profile of 96,796 single cells from 15 paired samples of primary tumors and axillary lymph nodes. We identified nine cancer cell subclusters including CD44 + / ALDH2 + /ALDH6A1 + breast cancer stem cells (BCSCs), which had a copy-number variants profile similar to that of normal breast tissue. Importantly, BCSCs existed only in primary tumors and evolved into metastatic clusters infiltrating into lymph nodes. Furthermore, transcriptome data suggested that NECTIN2-TIGIT-mediated interactions between metastatic breast cancer cells and tumor microenvironment (TME) cells, which promoted immune escape and lymph node metastasis. This study is the first to delineate the transcriptome profile of breast cancer lymph node metastasis using single-cell RNA sequencing. Our findings offer novel insights into the mechanisms underlying breast cancer metastasis and have implications in developing novel therapies to inhibit the initiation of breast cancer metastasis.

scholarly journals Case Report: Intercostal Lymph Node Metastasis: A Case Report and Review of the Literature

2021 ◽  
Vol 11 ◽  
Author(s):  
Yurong Zhou ◽  
Jinxuan Hou ◽  
Ning Meng ◽  
Staiculescu Daniel ◽  
Jiang Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Case Report ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Nodes ◽  
Cancer Metastasis ◽  
3D Visualization ◽  
Radical Mastectomy ◽  
Axillary Lymph ◽  
Node Metastasis

The axillary lymph nodes are the primary group responsible for lymphatic drainage in the breast and, consequently, are the most common location for breast cancer metastasis. However, lymphatic pathways running from the breast, via intercostal spaces, to parasternal lymph vessels have also been identified. According to the American Joint Committee on Cancer eighth edition manual, regional lymph node metastasis normally travels to the ipsilateral axillary, supraclavicular, subclavicular, and internal mammary lymph nodes. The presence of intercostal metastasis is out the range of these regional lymph nodes. It is very rare for intercostal lymph nodes to be the extra-axillary site of metastasis in breast cancer, and it has been little reported on in the literature. Despite its rarity, it has the capacity to adversely affect the prognosis of breast cancer and drastically influence treatment choice. Here, we analyze such a case, with a patient receiving a radical mastectomy and metastatic intercostal lymph node dissection due to the presence of intercostal lymph node metastasis indicated via MRI. Furthermore, the potential application of preoperative 3-dimensional (3D) visualization and surgical planning is also discussed.

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