scholarly journals Molecular characterization of Ribosomal DNA (ITS2) of hard ticks of Iran, in understanding the conspecificity of Dermacentor marginatus and D. niveus

2020 ◽  
Author(s):  
Parisa Soltan-Alinejad ◽  
Zahra Ramezani ◽  
Hamideh Edalat ◽  
Zakkyeh Telmadarraiy ◽  
Farrokh Dabiri ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Morphological Characteristics ◽  
Its2 Sequence ◽  
Dermacentor Marginatus ◽  
Hard Ticks ◽  
Wide Range ◽  
Different Populations ◽  
Additional Support

Abstract Objectives: Hard ticks (Acari: Ixodidae) are ectoparasites of human and animals and transmit a wide range of pathogens. The species identification of ticks is normally based on morphological keys. However, considering the importance of proper identification of the species and their bio-ecological characteristics, relying on morphological characteristics alone can be questionable. In this study, ITS2 and COI were selected for phylogenetic evaluation of Iranian species belonging to three genera: Dermacentor, Hyalomma and Rhipicephalus. Results: The ticks were from 10 populations from three different climatic and zoogeographical zones of Iran. The study of important morphological characteristics revealed notable differences in these characteristics between different populations of D. marginatus species.Contrary to the results of ITS2 sequence analysis which giving additional support for the view that D. niveus and D. marginatus should be viewed as one species only, the sequence analysis of COI and phylogeny favored the separation of the two species of D. niveus and D. marginatus. Given the greater importance of COI in identifying and distinguishing species, the Being two species should be considered.

scholarly journals Molecular characterization of Ribosomal DNA (ITS2) of hard ticks in Iran: understanding the conspecificity of Dermacentor marginatus and D. niveus

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Parisa Soltan-Alinejad ◽  
Zahra Ramezani ◽  
Hamideh Edalat ◽  
Zakkyeh Telmadarraiy ◽  
Farrokh Dabiri ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Tick Species ◽  
Morphological Characteristics ◽  
Blood Feeding ◽  
Its2 Sequence ◽  
Hard Tick ◽  
Dermacentor Marginatus ◽  
Hard Ticks ◽  
Morphological Studies ◽  
Veterinary Importance

Abstract Objectives Hard ticks (Acari: Ixodidae) are ectoparasites of medical and veterinary importance. They are obligate blood-feeding vectors with the ability to transmit a wide variety of pathogens. Standard morphological keys are normally used for the identification of tick species. However, considering the importance of accurate species identification and the determination of bio-ecological characteristics of species, relying on morphological keys alone can be questionable. In this study, two DNA fragments (ITS2 and COI) were selected for phylogenetic evaluation of Iranian hard tick species belonging to the genera Dermacentor, Hyalomma, and Rhipicephalus. Results 1229 specimens of Dermacentor marginatus, D. niveus, Hyalomma anatolicum, Rhipicephalus bursa, and R. sanguineuss.l constituting 11 populations were collected from three different climatic and zoogeographical zones in Iran. Morphological studies revealed notable differences in important morphological characteristics between different populations of D. marginatus. The results of ITS2 sequence analysis provided additional evidence which supports the conspecificity of D. niveus and D. marginatus. Contrary to this finding, the sequence analysis of COI and phylogeny favored the separation of the two species. Given the greater importance of COI in identifying and discriminating species, a possibility heterospecificity between the two species should be considered.

scholarly journals Molecular characterization of Ribosomal DNA (ITS2) of hard ticks in Iran: understanding the conspecificity of Dermacentor marginatus and D. niveus

2020 ◽  
Author(s):  
Parisa Soltan-Alinejad ◽  
Zahra Ramezani ◽  
Hamideh Edalat ◽  
Zakkyeh Telmadarraiy ◽  
Farrokh Dabiri ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Tick Species ◽  
Morphological Characteristics ◽  
Blood Feeding ◽  
Its2 Sequence ◽  
Hard Tick ◽  
Dermacentor Marginatus ◽  
Hard Ticks ◽  
Morphological Studies ◽  
Veterinary Importance

Abstract Objectives: Hard ticks (Acari: Ixodidae) are ectoparasites of medical and veterinary importance. They are obligate blood-feeding vectors with the ability to transmit a wide variety of pathogens. Standard morphological keys are normally used for the identification of tick species. However, considering the importance of accurate species identification and the determination of bio-ecological characteristics of species, relying on morphological keys alone can be questionable. In this study, two DNA fragments (ITS2 and COI) were selected for phylogenetic evaluation of Iranian hard tick species belonging to the genera Dermacentor, Hyalomma, and Rhipicephalus. Results: 1229 specimens of Dermacentor marginatus, D. niveus, Hyalomma anatolicum, Rhipicephalus bursa, and R. sanguineus constituting 11 populations were collected from three different climatic and zoogeographical zones in Iran. Morphological studies revealed notable differences in important morphological characteristics between different populations of D. marginatus.The results of ITS2 sequence analysis provided additional evidence which supports the conspecificity of D. niveus and D. marginatus. Contrary to this finding, the sequence analysis of COI and phylogeny favored the separation of the two species. Given the greater importance of COI in identifying and discriminating species, a possibility heterospecificity between the two species should be considered.

scholarly journals The genome of the endangered Macadamia jansenii displays little diversity but represents an important genetic resource for plant breeding

2021 ◽  
Author(s):  
Priyanka Sharma ◽  
Valentine Murigneux ◽  
Jasmine Haimovitz ◽  
Catherine J. Nock ◽  
Wei Tian ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Morphological Characteristics ◽  
Single Copy ◽  
Protein Coding ◽  
Core Eudicots ◽  
Wide Range ◽  
Genes Encoding ◽  
Long Read ◽  
In The Wild

SummaryMacadamia, a recently domesticated expanding nut crop in the tropical and subtropical regions of the world, is one of the most economically important genera in the diverse and widely adapted Proteaceae family. All four species of Macadamia are rare in the wild with the most recently discovered, M. jansenii, being endangered. The M. jansenii genome has been used as a model for testing sequencing methods using a wide range of long read sequencing techniques. Here we report a chromosome level genome assembly, generated using a combination of Pacific Biosciences sequencing and Hi-C, comprising 14 pseudo-molecules, with a N50 of 58 Mb and a total 758 Mb genome assembly size of which 56% is repetitive. Completeness assessment revealed that the assembly covered 96.9% of the conserved single copy genes. Annotation predicted 31,591 protein coding genes and allowed the characterization of genes encoding biosynthesis of cyanogenic glycosides, fatty acid metabolism and anti-microbial proteins. Re-sequencing of seven other genotypes confirmed low diversity and low heterozygosity within this endangered species. Important morphological characteristics of this species such as small tree size and high kernel recovery suggest that M. jansenii is an important source of these commercial traits for breeding. As a member of a small group of families that are sister to the core eudicots, this high-quality genome also provides a key resource for evolutionary and comparative genomics studies.

scholarly journals Isolation, Characterization, and Avenacin Sensitivity of a Diverse Collection of Cereal-Root-Colonizing Fungi

1999 ◽  
Vol 65 (8) ◽  
pp. 3364-3372 ◽  
Author(s):  
Jon P. Carter ◽  
John Spink ◽  
Paul F. Cannon ◽  
Michael J. Daniels ◽  
Anne E. Osbourn
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Ribosomal Dna ◽  
Dna Sequence Analysis ◽  
Fungal Communities ◽  
Fungal Isolates ◽  
Wide Range ◽  
Ribosomal Dna Sequence ◽  
Cereal Root ◽  
Basidiomycete Fungi

ABSTRACT A total of 161 fungal isolates were obtained from the surface-sterilized roots of field-grown oat and wheat plants in order to investigate the nature of the root-colonizing fungi supported by these two cereals. Fungi were initially grouped according to their colony morphologies and then were further characterized by ribosomal DNA sequence analysis. The collection contained a wide range of ascomycetes and also some basidiomycete fungi. The fungi were subsequently assessed for their abilities to tolerate and degrade the antifungal oat root saponin, avenacin A-1. Nearly all the fungi obtained from oat roots were avenacin A-1 resistant, while both avenacin-sensitive and avenacin-resistant fungi were isolated from the roots of the non-saponin-producing cereal, wheat. The majority of the avenacin-resistant fungi were able to degrade avenacin A-1. These experiments suggest that avenacin A-1 is likely to influence the development of fungal communities within (and possibly also around) oat roots.

scholarly journals Characterization of Two Undescribed Mucoralean Species with Specific Habitats in Korea

2018 ◽  
Author(s):  
Seo Hee Lee ◽  
Thuong T. T. Nguyen ◽  
Hyang Burm Lee
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Fungal Diversity ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Sequence Analyses ◽  
Saprotrophic Fungi ◽  
Gilbertella Persicaria ◽  
28S Ribosomal Dna

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. The strains were analyzed both for morphology and phylogeny based on the internal transcribed spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions. On the basis of their morphological characteristics and sequence analyses, isolates CNUFC-GWD3-9 and CNUFC-EGF1-4 were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively.To the best of our knowledge, there are no published literature records of these two genera in Korea.

Genetic diversity in perennial ryegrass (Lolium perenne) evaluated by hybridization with ribosomal DNA: implications for cultivar identification and breeding

1998 ◽  
Vol 131 (1) ◽  
pp. 23-30 ◽  
Author(s):  
K. M. F. WARPEHA ◽  
I. CAPESIUS ◽  
T. J. GILLILAND
Keyword(s):  
Genetic Diversity ◽  
Ribosomal Dna ◽  
Perennial Ryegrass ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Morphological Characteristics ◽  
Intergenic Spacer ◽  
Wide Range ◽  
Low Incidence ◽  
Potential Use

To date, little molecular genetic research has been conducted in Lolium and the current study is the first investigation of diversity in ribosomal DNA (rDNA) among perennial ryegrass cultivars. Ribosomal DNA probes derived from the intergenic spacer (IGS–M) and transcribed (COD–M) regions of mustard (Sinapis alba) were used to examine 35 perennial ryegrass (L. perenne L.) cultivars for genetic diversity using RFLP methodology. Analysis of digested genomic DNA on Southern blots revealed only 10 cultivars with unique RFLP patterns. Genetic diversity was exclusively associated with the IGS–M probe, as the cultivars were all indistinguishable when examined with the COD–M probe. This low incidence of distinctions was not expected, given that the 35 cultivars were known genetically distinct entities, representing the very wide range of the major phenotypic and physiological characteristics present in commercial stocks. Furthermore, the forage cultivars were proportionately less polymorphic in comparison to the amenity cultivars. This was also unexpected as amenity cultivars are recognized as a more closely clustered group than the forage cultivars, based on morphological characteristics. The potential use of rDNA for assessing cultivar diversity and breeding histories in perennial ryegrass is considered.

scholarly journals Synthesis and Morphological Characterization of Nanoporous Aluminum Oxide Films by Using a Single Anodization Step

Coatings ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 115 ◽  
Author(s):  
Florencia Bruera ◽  
Gustavo Kramer ◽  
María Vera ◽  
Alicia Ares
Keyword(s):  
Aluminum Oxide ◽  
Pore Diameter ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Morphological Properties ◽  
Pore Density ◽  
Synthesis Conditions ◽  
Interpore Distance ◽  
Wide Range

Nanoporous anodic aluminum oxide (AAO) films play an important role in nanotechnology due to their easily adjustable morphological properties and wide range of applications. Thus, a deep and systematic characterization of the morphological properties of these coatings is essential. The most important variables in the synthesis of nanoporous AAO films include the anodization voltage, nature, concentration and temperature of the electrolyte, which, combined, result in pores of different sizes and geometries. In the present work, AA 1050 alloy was used to synthesize AAO films, using 0.3 and 0.9 M oxalic acid as the electrolyte and combining different electrolyte temperatures (20, 30 and 40 °C) and anodizing voltages (30, 40 and 60 V), with the aim to correlate the morphological properties of the coatings with the synthesis parameters of a single anodization step. The coatings obtained were characterized by optical microscopy and scanning electron microscopy, determining pore diameter, interpore distance, pore density and coating thickness. The results showed that, by varying the anodic synthesis conditions, it is possible to obtain coatings with a pore diameter between 21 and 97 nm, an interpore distance between 59 and 138 nm, pore density between 2.8 × 1010 and 5.4 × 109 pores/cm2 and thicknesses between 15 and 145 µm. In this way, the right combination of synthesis variables allows synthesizing AAO coatings with morphological characteristics best suited to each particular application.

scholarly journals Molecular characterization of hard ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA

2009 ◽  
Vol 105 (5) ◽  
pp. 1479-1482 ◽  
Author(s):  
L. Chitimia ◽  
R. Q. Lin ◽  
I. Cosoroaba ◽  
P. Braila ◽  
H. Q. Song ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacers ◽  
Hard Ticks
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