A Research on the Determination of Phenological and Molecular Characterization in Open-pollinated Genotypes in Walnut

Abstract This research was carried out to reveal the phenological and genetic differences between the S-1/1 walnut genotype and 94 F1 genotypes obtained from this genotype with each other and with the maternal parent. In the phenological observations made, it was observed that bud burst in genotypes took 52 days, leafing 50 days, leaf yellowing 31 days, and defoliation date 27 days. When the maternal parent (S-1/1) and the genotypes were compared, it was found that there was a phenological variation of 75.54 % in budburst, 73.41 % in the leafing, 34.05 % in leaf yellowing, and 93.62 % in defoliation date, while the average variation was 69.15 %. In molecular genetic analyzes, 7 ISSR primers were used to determine genetic variations, as a result, 7 monomorphic and 45 polymorphic bands were obtained, and the rate of polymorphism was found to be 86.53 %. The average number of alleles was calculated to be 7.42. In genotypes, the polymorphism information content (PIC) value varied between 0.48 and 0.95, while the average PIC value was calculated to be 0.73. As a result of cluster analysis, it was seen that genotypes were divided into 2 main clusters and 2 subsets. At the end of the study, it was determined that the S-1/1 walnut genotype and F1 genotypes obtained from this genotype have a significant variation both phenologically and genetically.

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Molecular genetic analyses of oilseed Brassica germplasm: determination of life forms and germplasm management strategies by using microsatellite markers and FLOWERING LOCUS-C (FLC1 and FLC3) gene sequences

10.31274/rtd-180813-12484 ◽

2006 ◽

Author(s):

Von Mark V. Cruz

Keyword(s):

Microsatellite Markers ◽

Molecular Genetic ◽

Management Strategies ◽

Life Forms ◽

Germplasm Management ◽

Gene Sequences ◽

Genetic Analyses ◽

Flowering Locus C ◽

Flowering Locus

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Flash evaporation-gas chromatographic fingerprints with hierarchical cluster analysis for the determination of cigarette flavors

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2011.00549 ◽

2011 ◽

Vol 29 (6) ◽

pp. 549-553

Author(s):

Jian JIANG ◽

Jun YANG ◽

Fangfang HUANG ◽

Shiqiang XU ◽

Xiaoqing WANG ◽

...

Keyword(s):

Cluster Analysis ◽

Hierarchical Cluster Analysis ◽

Hierarchical Cluster ◽

Flash Evaporation ◽

Chromatographic Fingerprints

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Molecular Fingerprinting of Indian Medicinal Tree Sara Asoca using RAPD Markers

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2874 ◽

2021 ◽

Vol 17 (4) ◽

pp. 701-705

Author(s):

Shailendra Singh Yadav ◽

Ashwini A. Waoo

Keyword(s):

Cluster Analysis ◽

Genomic Dna ◽

Rapd Markers ◽

Conservation Strategies ◽

Molecular Fingerprinting ◽

Initial Experiment ◽

Medicinal Tree ◽

Average Percentage ◽

Saraca Asoca ◽

Polymorphic Bands

Saraca asoca is an important medicinal tree facing a serious problem of reduction from its instinctivetenancy in India.Before formulation of conservation strategies for geographical protection of S. asoca genotypes available in India, it is necessary to characterize them. In the current study, the RAPD markers have been utilized effectively for categorization of S. asoca collected from 15 diverse sites of India. An initial experiment on the amplification suitability of genomic DNA samples of four S. asoca was done with 35RAPD primers. Among them only twenty sixproved their efficiency in two times repeat amplification.Total 146 bands were amplified and out of these 97 bands were found to be polymorphic. The average numbers of total band was 5.61 while average numbers of polymorphic bands was 3.73. The numbers of bands produced per primer ranged from 3 (OPE-15) to 8 (RUF205). Among all studied markers the highest percentage (100%) of polymorphism was demonstrated by only one marker (OPE-06). The lowest percent of polymorphicm (20%) was demonstrated by marker RUF211. The average percentage of polymorphism was 66.44%. Cluster analysis grouped all the S. asoca genotypes under study into two groups. Grouping of genotypes according to their sites of collection demonstrates higher similarity among or between them. The results obtained in the current study may help to formulate conservation strategies for the conservation of S. asoca genotypes.

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Phenotypes of professional bronchial asthma from the standpoint of spirometry

Terapevt (General Physician) ◽

10.33920/med-12-2103-07 ◽

2021 ◽

pp. 43-54

Author(s):

Antonina G. Baykova ◽

Marina Yuryevna Vostroknutova ◽

Natalia A. Ostryakova ◽

Tatyana Mikhailovna Kiryushina

Keyword(s):

Comparative Analysis ◽

Bronchial Asthma ◽

Clinical Stage ◽

Molecular Genetic ◽

Control Group ◽

Forced Exhalation ◽

Choice Of Treatment ◽

Genetic Examination

The aim of the study was to conduct a comparative analysis of spirometric indicators of respiration in various phenotypes of occupational bronchial asthma. Materials and methods. At the clinical stage of the work, a comprehensive clinical, radiological, spirographic, echocardiographic, immunological and molecular genetic examination of 170 patients of the main groups and 50 individuals of the control group was carried out. The results of the study. Dynamic determination of the speed indicators of forced exhalation in various phenotypes of occupational bronchial asthma can improve the diagnosis of obstructive disorders in this pathology, optimize the choice of treatment tactics, and predict the course of this pathology.

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Topical Review: Cortical Malformation and Pediatric Epilepsy: A Molecular Genetic Approach

Journal of Child Neurology ◽

10.1177/08830738040190030501 ◽

2004 ◽

Vol 19 (3) ◽

pp. 300-303

Author(s):

Ganeshwaran H. Mochida

Keyword(s):

Human Brain ◽

Cell Fate ◽

Molecular Genetic ◽

Pediatric Epilepsy ◽

Causative Gene ◽

Cortical Malformation ◽

Neuronal Progenitor ◽

Cortical Patterning ◽

Low Incidence

Genetic malformations of the cerebral cortex are important causes of neurologic morbidity in children because they are often associated with developmental delay, motor disturbances (cerebral palsy), and epilepsy. Primary autosomal recessive microcephaly is a cortical malformation with a low incidence of epilepsy. One of its causative genes, ASPM, might play an important role in regulating proliferation of neuronal progenitor cells. Mutations in ASPM do not seem to affect later stages of cortical development, such as neuronal migration, and this might be responsible for the low epileptogenicity of this malformation. ASPM might also have played an important role in the evolutionary expansion of the human brain. Bilateral frontoparietal polymicrogyria, on the other hand, is a highly epileptogenic malformation. Its causative gene, GPR56 , is also expressed in the neurogenic regions of the cortex, but its primary function might be in the determination of cell fate and/or cortical patterning. Further studies of these genes will likely lead to a better understanding of human brain development and epilepsy. ( J Child Neurol 2005;20:300—303).

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Effect of anchor and core sequence in microsatellite primers on flax fingerprinting patterns

The Journal of Agricultural Science ◽

10.1017/s0021859601001162 ◽

2001 ◽

Vol 137 (1) ◽

pp. 37-44 ◽

Cited By ~ 19

Author(s):

I. WIESNER ◽

D. WIESNEROVÁ ◽

E. TEJKLOVÁ

Keyword(s):

Cluster Analysis ◽

Large Scale ◽

Annealing Temperature ◽

Microsatellite Primers ◽

Microsatellite Data ◽

Core Group ◽

The Core ◽

Polymorphic Bands ◽

Flax Cultivar ◽

Pcr Conditions

The aim of this study was to select the best arrangements of MP–PCR (microsatellite-primed PCR) for routine large-scale fingerprinting of flax cultivars. We found optimum PCR conditions for the application of five previously published primers (PCT1–PCT5) to flax cultivar fingerprinting. We modified to optimum MP–PCR which was targeted to flax tetrameric [GATA] microsatellite loci specified by primer PCT6. We found that after a reamplification PCR step was involved we were able to generate highly discriminating fingerprinting patterns, which distinguished all eight flax cultivars individually. In particular primers 3PCT1 and 3PCT2 were promising for future large-scale fingerprinting due to the production of most polymorphic bands. Increasing annealing temperature within a temperature profile helped to generate new polymorphisms within flax microsatellite patterns especially with primer 3PCT2. Using this primer we succeeded in generating new polymorphic bands after increasing annealing temperature from 55 °C to 60 °C, and to 65 °C. A cluster analysis of flax cultivars was performed based on microsatellite data. The core group of eight flax cultivars was clustered into two homogeneous subclusters. A lower level of cultivar clustering within subclusters was not detected.

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Intercomparison Study on the Determination of Single Administration Toxicity in Rats

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/62.4.864 ◽

1979 ◽

Vol 62 (4) ◽

pp. 864-873

Author(s):

William J Hunter ◽

W Lingk ◽

P Recht

Keyword(s):

Pilot Study ◽

Significant Variation ◽

Single Administration ◽

Ld50 Value ◽

Intercomparison Study

Abstract In 1977, the Commission of the European Communities initiated an intercomparison study to determine the single administration oral LD50 value in rats of each of 5 chemicals with the aims of comparing experimentation technologies, determining the degree of variation in the results and the various parameters used to establish the LD50 value, and establishing a common protocol for the determination of the LD50 value. Sixty-five laboratories in 8 countries took part in the first study. The significant variation in protocol may have led to the large interlaboratory variation observed in the results. Therefore, participating laboratories carried out a second study using a common protocol, preceded by a pilot study. A total of 100 laboratories in 13 countries participated. A majority of results of the second study are currently being analyzed, and the indications are that the interlaboratory variation has been significantly reduced.

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Determination of Carotene in Fresh Forages and Silages Following Freeze-Drying and Grinding

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.4.828 ◽

1967 ◽

Vol 50 (4) ◽

pp. 828-830

Author(s):

J T Gillingham

Keyword(s):

Freeze Drying ◽

Carotene Content ◽

Average Variation ◽

Aoac Method

Abstract The carotene content of fresh forages and silages has been analyzed by two methods: AOAC method 39.015(a) and the Bickoff et al. method modified to include freeze-drying and grinding of the sample and chromatographing only an aliquot after extraction. Data on orchardgrass, fescue, clover-grass mixtures, and silage samples indicated that the method described is accurate and reproducible. The average variation is ± 8μg/g and identical values in duplicate determinations are recorded in 16 out of 34 values.

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