scholarly journals Serine–Arginine Protein Kinase-like Protein, SrpkF, Stimulates Both Cellobiose-responsive and D-Xylose-Responsive Signaling Pathways in Aspergillus Aculeatus

2021 ◽  
Author(s):  
Ryohei Katayama ◽  
Natsumi Kobayashi ◽  
Takashi Kawaguchi ◽  
Shuji Tani
Keyword(s):  
Protein Kinase ◽  
Signaling Pathways ◽  
Carbon Sources ◽  
Fold Increase ◽  
Cellulolytic Enzyme ◽  
Induction Phase ◽  
Aspergillus Aculeatus ◽  
Kinase Gene ◽  
Protein Kinase Gene ◽  
Overexpressing Strain

Abstract To elucidate the regulatory mechanisms of various cellulolytic enzyme genes in Aspergillus aculeatus , we identified one mutant that reduced the expression of FIII-avicelase ( chbI ) in response to cellulose from 12,000 A . aculeatus T-DNA-inserted mutants. The T-DNA inserted into a putative protein kinase gene similar to AN10082 in A . nidulans , the serine–arginine protein kinase F, SrpkF. The fold increase in srpkF gene expression in response to various carbon sources was 2.3 (D-xylose), 44 (Avicel®), 59 (Bacto™ Tryptone), and 98 (no carbon) compared with D-glucose. The deletion of srpkF in A . aculeatus resulted in a significant reduction in the cellulose-responsive expression of chbI , hydrocellulase ( cel7b ), and FIb-xylanase ( xynIb ) genes at an early induction phase. However, the srpkF deletion did not affect the expression of xynIb in response to D-xylose. Furthermore, the srpkF -overexpressing strain that expresses the srpkF gene at levels from four- to nine-fold higher than the control strain stimulated the expression of cbhI and cel7b in response to cellobiose and the FI-carboxymethyl cellulase gene ( cmc1 ) and xynIb in response to xylose. The expression of cbhI and cel7b is regulated by a transcriptional activator, ManR, and the expression of cmc1 and xynIb is regulated by XlnR. Our data demonstrate that SrpkF can stimulate both the ManR- and XlnR-dependent signaling pathways in response to cellobiose and D-xylose in A . aculeatus .

Characterization of a protein kinase gene from two Chlorella viruses

1995 ◽  
Vol 35 (3) ◽  
pp. 291-305 ◽  
Author(s):  
Quideng Que ◽  
James L. Van Etten
Keyword(s):  
Protein Kinase ◽  
Kinase Gene ◽  
Protein Kinase Gene

scholarly journals The Evolutionary History and Diverse Physiological Roles of the Grapevine Calcium-Dependent Protein Kinase Gene Family

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e80818 ◽  
Author(s):  
Fei Chen ◽  
Marianna Fasoli ◽  
Giovanni Battista Tornielli ◽  
Silvia Dal Santo ◽  
Mario Pezzotti ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Gene Family ◽  
Evolutionary History ◽  
Dependent Protein Kinase ◽  
Kinase Gene ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Protein Kinase Gene ◽  
Calcium Dependent Protein Kinase

scholarly journals Distribution and evolution of CTG repeats at the myotonin protein kinase gene in human populations.

1996 ◽  
Vol 6 (2) ◽  
pp. 142-154 ◽  
Author(s):  
R Deka ◽  
P P Majumder ◽  
M D Shriver ◽  
D N Stivers ◽  
Y Zhong ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Populations ◽  
Kinase Gene ◽  
Ctg Repeats ◽  
Protein Kinase Gene

Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 in Rice

Rice Science ◽  
2010 ◽  
Vol 17 (4) ◽  
pp. 269-275 ◽  
Author(s):  
Wei-hong LIANG ◽  
Jia-jia BI ◽  
Wei-feng PENG ◽  
Fan ZHANG ◽  
Hong-hao SHI ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Expression Analysis ◽  
Mitogen Activated Protein Kinase ◽  
Cloning And Expression ◽  
Kinase Gene ◽  
Mitogen Activated Protein ◽  
Protein Kinase Gene

tkl is the avian homolog of the mammalian lck tyrosine protein kinase gene

1992 ◽  
Vol 12 (3) ◽  
pp. 1226-1233
Author(s):  
L M Chow ◽  
M J Ratcliffe ◽  
A Veillette
Keyword(s):  
Protein Kinase ◽  
T Lymphocytes ◽  
Gene Product ◽  
Lymphoid Tissues ◽  
Protein Kinase Activity ◽  
Kinase Gene ◽  
Rnase Protection ◽  
Tyrosine Protein Kinase ◽  
Protein Kinase Gene ◽  
Unique Domain

We have tested the possibility that tkl, a partially characterized avian tyrosine protein kinase gene, is the chicken homolog of lck, a lymphocyte-specific mammalian gene. Using polymerase chain reactions, we have cloned sequences encoding the previously unidentified amino terminus of the tkl gene product. The newly defined unique domain of Tkl displayed significant identity (68%) to the equivalent region of the mammalian lck gene product, p56lck. This identity included a glycine residue at position 2 (present in all Scr-related tyrosine protein kinases) and a cysteine motif at positions 20 and 23, which allows binding of p56lck to CD4 and CD8 in mammalian T lymphocytes. A specific RNase protection assay revealed that, in contrast to a previous report (K. Strebhardt, J. I. Mullins, C. Bruck, and H. Rübsamen-Waigmann, Proc. Natl. Acad. Sci. USA 84:8778-8782, 1987), tkl expression is restricted to the lymphoid tissues thymus and spleen. Moreover, the absence of tkl transcripts in the bursa of Fabricius suggested that this gene is expressed in avian T lymphocytes but not in B lymphocytes. A polyclonal rabbit antiserum raised against the unique domain of Tkl recognized a 56-kDa polypeptide with associated protein kinase activity from avian thymus-derived cells. Additional studies showed that p56tkl is structurally similar to mammalian p56lck and that it is physically associated with the avian CD4 and CD8 T-cell surface antigens. It was also determined that tkl transcripts have one major type of 5' untranslated region (UTR), which differs greatly from the two known 5' UTRs of mammalian lck mRNAs.(ABSTRACT TRUNCATED AT 250 WORDS)

The human protein kinase gene PKX1 on Xp22.3 displays Xp/Yp homology and is a site of chromosomal instability

1995 ◽  
Vol 4 (5) ◽  
pp. 869-878 ◽  
Author(s):  
A. Klink ◽  
K. Schiebel ◽  
M. Winkelmann ◽  
E. Rao ◽  
B. Horsthemke ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Chromosomal Instability ◽  
Human Protein ◽  
Kinase Gene ◽  
Human Protein Kinase ◽  
Protein Kinase Gene ◽  
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