Co-Expression of Candidate Genes Regulating Growth Performance and Carcass Traits of Barki Lambs in Egypt

Abstract Sheep are considered one of the main sources of animal protein in Egypt while surviving harsh desert conditions. Therefore, the aim of present study was to link the gene expression profile of selected candidate genes with growth performance and carcass traits of Barki lambs. Thirty-eight Barki lambs were kept and fed individually after weaning till 12 months of age. Lambs were divided into 3 groups (fast, intermediate and slow growing) according to growth performance. Carcass traits were recorded directly after slaughtering. Three samples were taken from different body tissues (eye muscle, liver and fat tail) of each group, directly during slaughtering and stored at -80°C until RNA isolation. Real-time PCR was used to profile selected candidate genes (RPL7, CTP1, FABP4, ADIPOQ and CAPN3) and GAPDH was used as a housekeeping gene. The results indicated that, the final body weight was significantly (P ≤ 0.05) greater in fast (49.9 Kg) and intermediate (40.7 Kg) compared to slow growing animals (30.8 Kg). The hot carcass weight was heavier (P ≤ 0.05) in fast, intermediate (24.57 and 19.07 Kg) than low growing lambs (15.10 Kg). The blood profiles of T3 and T4 hormones showed no significant different among fast (1.1 ng/ml and 8.5 µg/dl), intermediate (0.6 ng/ml and 12.4 µg/dl) and slow growing lambs (1.0 ng/ml and 10.5 µg/dl). In addition, the total protein level was not differ in fast (5.94 g/dl), intermediate (6.52 g/dl) and slow growing lambs (6.9 g/dl). Genes involved in protein biosynthesis (RPL7), fatty acid oxidation (CPT1) and lipolysis (FABP4) were up regulated in fast and intermediate growing lambs in all studied tissues. While, gene-regulating lipogenesis (ADIPOQ) was expressed similarly in fat and liver tissues, but increased its expression in muscle of fast and intermediate growing lambs. Expression of CAPN3 was increased in fast and intermediate growing compared to slow growing lambs. In conclusion, the current study indicated a link between variation in gene expression profile in main body tissues with growth performance and carcass weight of Barki lambs, which induced by the genetic makeup however; this hypothesis required a large population for validation. In addition, the transcriptional profile of CPT1, FABP4 RPL7 and CAPN3 are linked with growth performance and carcass traits of Barki lambs, providing an evidence for the importance of co-expression of these genes in body main tissues involved in this process.

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Co-expression of candidate genes regulating growth performance and carcass traits of Barki lambs in Egypt

10.20944/preprints202106.0199.v1 ◽

2021 ◽

Author(s):

Nasser Ghanem ◽

Mohamed Zayed ◽

Ismail Mohamed ◽

Mona Mohammady ◽

M.F. Shehata

Keyword(s):

Growth Performance ◽

Candidate Genes ◽

Protein Biosynthesis ◽

Carcass Traits ◽

Acid Oxidation ◽

Main Body ◽

Final Body Weight ◽

Blood Profile ◽

Body Tissues ◽

Slow Growing

The aim of present study was to link the gene expression profile of selected candidate genes with blood profile, growth performance and carcass traits of Barki lambs. Thirty-eight Barki lambs were divided into 3 groups (fast, intermediate and slow growing) according to growth perfor-mance. Body tissues (muscle, liver and fat) were taken from for RNA isolation and Real-time PCR. The results indicated that, the final body weight hot carcass weight were heavier (P ≤ 0.05) in fast (49.9 Kg and 24.57) than intermediate (40.7 and 19.07 Kg) and slow (30.8 and 15.10 Kg) growing animals. The blood profile of total protein, total lipids, calcium, T3 and T4 hormones did not differ among sheep groups. Genes involved in protein biosynthesis (RPL7), fatty acid oxidation (CPT1) and lipolysis (FABP4) were up regulated in fast and intermediate growing lambs in all studied tissues. While, gene-regulating lipogenesis (ADIPOQ) was expressed simi-larly in fat and liver tissues, but increased its expression in muscle of fast and intermediate growing lambs. Expression of CAPN3 was increased in fast and intermediate growing compared to slow growing lambs. In conclusion, the current study providing an evidence for the im-portance of co-expression of these genes in main body tissues linked with growth performance of Barki lambs.

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Analysis of the retinal gene expression profile after hypoxic preconditioning identifies candidate genes for neuroprotection

BMC Genomics ◽

10.1186/1471-2164-9-73 ◽

2008 ◽

Vol 9 (1) ◽

pp. 73 ◽

Cited By ~ 39

Author(s):

Markus Thiersch ◽

Wolfgang Raffelsberger ◽

Rico Frigg ◽

Marijana Samardzija ◽

Andreas Wenzel ◽

...

Keyword(s):

Gene Expression ◽

Candidate Genes ◽

Gene Expression Profile ◽

Expression Profile ◽

Hypoxic Preconditioning

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Identification of potential candidate genes for hypertensive nephropathy based on gene expression profile

BMC Nephrology ◽

10.1186/s12882-016-0366-8 ◽

2016 ◽

Vol 17 (1) ◽

Cited By ~ 5

Author(s):

Zhi Chen ◽

Hao Wu ◽

Guohua Wang ◽

Ye Feng

Keyword(s):

Gene Expression ◽

Candidate Genes ◽

Gene Expression Profile ◽

Expression Profile ◽

Potential Candidate ◽

Hypertensive Nephropathy

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Integrating the analysis of transcriptome-wide association study and gene expression profile to identify genes associated with spondyloarthritis

10.21203/rs.3.rs-936677/v1 ◽

2021 ◽

Author(s):

Jiawen Xu ◽

Haibo Si ◽

Yi Zeng ◽

Yuangang Wu ◽

Shaoyun Zhang ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Association Study ◽

Candidate Genes ◽

Gene Expression Profile ◽

Expression Profile ◽

Whole Blood ◽

Genome Wide Association Study ◽

Bone Diseases ◽

Functional Enrichment

Abstract Background Spondyloarthritis(SpA) is a group of multi-factorial bone diseases influenced by genetic factors, environment and lifestyles. However, the genetic and pathogenic mechanism of SpA is still elusive. Methods Firstly, the tissue-specific transcriptome-wide association study (TWAS) of SpA was performed by utilizing the genome-wide association study (GWAS, including 3966 SpA patients and 452264 controls) summary data and gene expression weights of the whole blood and skeletal muscle. Secondly, the SpA-associated genes identified by TWAS were further compared with the differentially expressed genes(DEGs) detected by gene expression profile of SpA acquired from the Gene Expression Omnibus database (GEO, accession number:GSE58667). Finally, FUMA and Metascape tools were used to conduct gene functional enrichment and annotation analysis. Results TWAS detected 28 significant genes associated with SpA both in the whole blood and skeletal muscle, such as CTNNAL1 (PSM=0.0304, PWB=0.0096). Further comparing with gene expression profile of SpA, we identified 20 candidate genes which overlapped in TWAS, such as MCM4 (PTWAS=0.0132, PDEG=0.0275), KIAA1109 (PTWAS=0.0371,PDEG=0.0467). The enrichment analysis of the genes identified by TWAS detected 93 significant GO terms 33 and KEGG pathways, such as mitochondrion organization (GO:0007005, log10(P)= -4.29) and axon guidance(hsa04360, log10(P)= -4.26). Conclusion We identified multiple candidate genes genetically related to SpA. Our study may provide some novel clues for the further study of the genetic mechanism, diagnosis and treatment of SpA.

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Influence of Crossbreeding of Ghezel Ram With Grey Shirazi Ewe on Growth Performance, Feed Efficiency and Carcass Traits of Fattening Lambs

10.21203/rs.3.rs-642724/v1 ◽

2021 ◽

Author(s):

Abdolhamid Karimi ◽

Mohammad Javad Abarghuei ◽

Alidad Boostani

Keyword(s):

Body Weight ◽

Growth Performance ◽

Feed Efficiency ◽

Average Daily Gain ◽

Carcass Traits ◽

Live Weight ◽

Carcass Weight ◽

Feed Conversion ◽

Final Body Weight ◽

Group 12

Abstract In this investigation, performance, feed efficiency and carcass traits of 48 weaned crossbred Grey Shirazi × Ghezel and purbred Grey Shirazi fattening lambs (mean live weight, 28.5 ± 0.5 kg and age, 115 ± 5 days) were evaluated. Lambs were divided into 4 groups contain the GM group (12 purebred male lambs of Grey Shirazi ewe × Grey Shirazi ram), the GF group (12 purebred female lambs of Grey Shirazi ewe × Grey Shirazi ram), the CM group (12 crossbred male lambs of Grey Shirazi ewe × Ghezel ram) and the CF group (12 crossbred female lambs of Grey Shirazi ewe × Ghezel ram). The dry matter intakes (DMI), average daily gain (ADG) and carcass traits in the lambs were determined. The DMI was greater in CF lambs compared to GF lambs (P < 0.001). Feed conversion ratio (FCR) was higher in male lambs comparing to female lambs (P = 0.001). The final body weight, ADG, live weight at the slaughtering and hot carcass weight (HCW) were lower in GF lambs compared to another lambs (P < 0.001, P = 0.003). Cold carcass weight (CCW) in CM lambs was higher than in other groups. The Longissimus dorsi (LD) area was higher in crossbreed male lambs (P = 0.001). Crossbreeding improved grow performance (final body weight and ADG) in male and female lambs. Carcass trait was improved by crossbreeding especially in male lambs. Sex effect on growth performance and carcass traits was noticeable and male lambs had superior growth.

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