scholarly journals Co-expression of candidate genes regulating growth performance and carcass traits of Barki lambs in Egypt

Author(s):  
Nasser Ghanem ◽  
Mohamed Zayed ◽  
Ismail Mohamed ◽  
Mona Mohammady ◽  
M.F. Shehata

The aim of present study was to link the gene expression profile of selected candidate genes with blood profile, growth performance and carcass traits of Barki lambs. Thirty-eight Barki lambs were divided into 3 groups (fast, intermediate and slow growing) according to growth perfor-mance. Body tissues (muscle, liver and fat) were taken from for RNA isolation and Real-time PCR. The results indicated that, the final body weight hot carcass weight were heavier (P ≤ 0.05) in fast (49.9 Kg and 24.57) than intermediate (40.7 and 19.07 Kg) and slow (30.8 and 15.10 Kg) growing animals. The blood profile of total protein, total lipids, calcium, T3 and T4 hormones did not differ among sheep groups. Genes involved in protein biosynthesis (RPL7), fatty acid oxidation (CPT1) and lipolysis (FABP4) were up regulated in fast and intermediate growing lambs in all studied tissues. While, gene-regulating lipogenesis (ADIPOQ) was expressed simi-larly in fat and liver tissues, but increased its expression in muscle of fast and intermediate growing lambs. Expression of CAPN3 was increased in fast and intermediate growing compared to slow growing lambs. In conclusion, the current study providing an evidence for the im-portance of co-expression of these genes in main body tissues linked with growth performance of Barki lambs.

2021 ◽  
Author(s):  
Nasser Ghanem ◽  
Mohamed Zayed ◽  
Ismail Mohamed ◽  
Mona Mohammady ◽  
Mohamed Farag Shehata

Abstract Sheep are considered one of the main sources of animal protein in Egypt while surviving harsh desert conditions. Therefore, the aim of present study was to link the gene expression profile of selected candidate genes with growth performance and carcass traits of Barki lambs. Thirty-eight Barki lambs were kept and fed individually after weaning till 12 months of age. Lambs were divided into 3 groups (fast, intermediate and slow growing) according to growth performance. Carcass traits were recorded directly after slaughtering. Three samples were taken from different body tissues (eye muscle, liver and fat tail) of each group, directly during slaughtering and stored at -80°C until RNA isolation. Real-time PCR was used to profile selected candidate genes (RPL7, CTP1, FABP4, ADIPOQ and CAPN3) and GAPDH was used as a housekeeping gene. The results indicated that, the final body weight was significantly (P ≤ 0.05) greater in fast (49.9 Kg) and intermediate (40.7 Kg) compared to slow growing animals (30.8 Kg). The hot carcass weight was heavier (P ≤ 0.05) in fast, intermediate (24.57 and 19.07 Kg) than low growing lambs (15.10 Kg). The blood profiles of T3 and T4 hormones showed no significant different among fast (1.1 ng/ml and 8.5 µg/dl), intermediate (0.6 ng/ml and 12.4 µg/dl) and slow growing lambs (1.0 ng/ml and 10.5 µg/dl). In addition, the total protein level was not differ in fast (5.94 g/dl), intermediate (6.52 g/dl) and slow growing lambs (6.9 g/dl). Genes involved in protein biosynthesis (RPL7), fatty acid oxidation (CPT1) and lipolysis (FABP4) were up regulated in fast and intermediate growing lambs in all studied tissues. While, gene-regulating lipogenesis (ADIPOQ) was expressed similarly in fat and liver tissues, but increased its expression in muscle of fast and intermediate growing lambs. Expression of CAPN3 was increased in fast and intermediate growing compared to slow growing lambs. In conclusion, the current study indicated a link between variation in gene expression profile in main body tissues with growth performance and carcass weight of Barki lambs, which induced by the genetic makeup however; this hypothesis required a large population for validation. In addition, the transcriptional profile of CPT1, FABP4 RPL7 and CAPN3 are linked with growth performance and carcass traits of Barki lambs, providing an evidence for the importance of co-expression of these genes in body main tissues involved in this process.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 436-437
Author(s):  
Arquimedes Lima Junior ◽  
Murillo A Meschiatti ◽  
Vinicius N Gouvêa ◽  
Victor Dantas ◽  
Daniel Silva Antonelo ◽  
...  

Abstract The objective of this study was to evaluate the growth performance and carcass traits of finishing bulls fed diets containing, on dietary dry matter basis, different levels of corn wet distillers bran plus solubles (WDBS; 0, 15, 30 or 45%) added with or without corn oil (CO; 0 and 3%). A total of 205 Nellore bulls (390 ± 25 kg initial BW) were blocked by initial body weight, distributed in 40 pens and pens within weight block were randomly assigned to treatments in a 2 × 4 factorial arrangement. Diets were isonitrogenous and contained 8.5% sugarcane bagasse and 91.5% concentrate (ground corn, soybean meal, pelleted citrus pulp, urea, mineral). The WDBS (FlexyDDG®) and the corn oil were from SJC Bioenergia (Quirinópolis, Goiás, Brazil). Data were analyzed using the PROC MIXED procedure of SAS. An interaction between CO × WDBS levels was observed for dry matter intake (DMI; P = 0.032). In diets without CO, increasing levels of WDBS linearly (P = 0.004) reduced the DMI, but in diets containing 3% CO, increasing levels of WDBS quadraticaly affected the DMI (P = 0.002). No effects of CO, WDBSF levels or CO × WDBS levels were observed for final body weight, average daily gain, hot carcass weight and dressing percentage. Feed efficiency tended (P = 0.07) to be greater for bulls fed diets containing CO (0.154 vs. 0.144). Increasing levels of WDBS tended (P = 0.09) to increase the feed efficiency. In summary, increasing levels of WDBS in finishing diets linearly (in diets without CO) or quadratically (in diets with CO) reduces the dry matter intake. Adding CO and WDBS tend to increase feed efficiency.


2016 ◽  
Vol 16 (2) ◽  
pp. 489-505 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Elwy Ali Ashour ◽  
Fayez Mohamed Reda

Abstract This study was performed to investigate the effects of dietary supplementation with garlic and turmeric powder as growth promoter agents on performance, carcass traits, serum biochemistry, and antioxidant enzyme activities of growing rabbits. A total of 112 New Zealand White rabbits (NZW) at 5 weeks of age were randomly assigned to seven treatments with four replicates. The dietary treatments consisted of 7 groups as follows; the basal diet as control, phytogenic additives groups were supplemented with 2, 4, and 6 g/kg garlic or turmeric powder added to the basal diet. There were no linear and quadratic differences (P<0.05) in growth performance after garlic or turmeric supplementation at all studied ages. Compared with the control group, supplementation of diets with garlic or turmeric linearly and quadratically elevated immunity biomarkers such as total protein (TP), albumin (AL) and immunoglobulin (IgG) levels and decreased (linearly and quadratically, P<0.05) aspartate aminotransferase (AST), alanine aminotransferase (ALT ), triglyceride (TG) and total cholesterol (TC) levels in rabbit serum. However, TP, AL, globulin (GL), IgG and IgM levels were linearly and quadratically enhanced with increasing turmeric levels versus the control diet. Hepatic superoxide dismutase (SOD), catalase (CAT ) and glutathione peroxidase GSH-Px activities as well as reduced glutathione (GSH) concentrations were linearly and quadratically (P<0.05) improved in garlic or turmeric additives fed groups. While MDA concentration was statistically (linearly, P= 0.022) reduced in comparison with the control group. It could be concluded that garlic or turmeric supplementation (2, 4 and 6 g/kg) did not linearly and quadratically affect growth performance but improved the immunity responses and lowered the lipid profile in blood and lipid peroxidation in liver and increased hepatic antioxidant activity in treated rabbits.


2020 ◽  
Vol 47 (2) ◽  
pp. 264-270
Author(s):  
A. I. Ukanwoko ◽  
N. C. Douglas ◽  
N. C. Igwe

This study was conducted to evaluate the growth performance and blood profile of grazedRed Sokoto bucks supplemented with dried ripe plantain peels. Nine bucks were used for thisexperiment. The goats were apportioned to three treatment diets and dried ripe plantainpeels incorporated at 0%, 5% and 10% inclusion levels for T , T and T respectively. The 1 2 3,bucks were taken out for grazing in the morning (9.00am – 12noon) and thereafter fed theexperimental diet. The experiment lasted for eight (8) weeks. The animals were weighed atthe beginning of the experiment and weekly afterwards. Feed intake was calculated as thedifference between feed served and refusal. Blood samples of the bucks were collected todetermine the blood profile using disposable syringes and sterile needles. Results showedthat the final body weight, body weight gain and feed conversion ratio (FCR) differedsignificantly (P<0.05). The final body weight, body weight gain and FCR were 18.02kg,2.01kg, 3.42; 20.75kg, 3.89kg, 1.26 and 21.35kg, 4.80kg, 1.10 for T , T and T respectively. 1 2 3,Platelet, Alkaline phosphatase (ALP), total protein (TP), urea and creatinine values differedsignificantly (P<0.05). The highest platelet was recorded for bucks on T (212.91x10 3/ul) 3while the lowest was for bucks on T (202.30x103/ul). ALP, TP, urea and creatinine values 1were 29.00u/l, 60.00g/l, 8.00m/mol,72.50m/mol; 30.30u/l, 52.30g/l, 10.00m/mol,141.00m/mol and 42.00u/l, 53.30g/l, 9.70m/mol, 112.30m/mol for T , T and T , respectively. 1 2 3The inclusion of supplemental dried ripe plantain peels in the diet of Red Sokoto bucksenhanced the body weight and did not have any adverse effect on their blood profile.


2021 ◽  
Vol 43 (1) ◽  
pp. 254-262
Author(s):  
A. O. Aniebo

The effect of dietary treatments on growth performance, haematological and biochemical indices of finisher broilers as well as economics of broiler production were determined in this fermented cassava flour/blood mixture code-named "cassablood". The control diet (T1) contained 0% cassablood, while diets 2, 3 and 4 contained 20%, 25% and 30% cassablood respectively. 120 four weeks old Anak broiler chicken were randomly allocated the 4 diets with 30 birds per treatment replicated 3 times with ten 10 broilers per replicate in a completely randomized design (CRD). Treatment effects on final body weight, final weight gain, feed intake and seed conversion ratio were significant (P<0.05). Birds fed cassablood based diets compared favourably with the control diet. 20% cassablood inclusion performed equally with the control diet in feed intake, body weight gain and feed conversion ratio.All dietary groups also showed no significant differences (P>0.05) in most haematological and serum biochemical indices of broilers except in cholesterol, glucose and triglyceride contentswhichdiffered (P<0.05) significantly. Similarly, chicken on 20% and 25% inclusion levels produced significantly (P < 0.05) higher economic gains in terms of feed cost per kilogram of broiler among all the treatments. Therefore, 20 - 25% cassablood dietary inclusion level was recommended in broiler diet for production and profit maximization.  


2021 ◽  
Vol 8 ◽  
Author(s):  
Xichen Zhao ◽  
Yating Liu ◽  
Hao Ding ◽  
Pan Huang ◽  
Yulong Yin ◽  
...  

The Huanjiang mini-pig is a Chinese local breed and, the optimal dietary crude protein (CP) level for this breed is still unknown. Therefore, the present study was conducted to investigate its optimum dietary CP level upon the growth performance, serum biochemical parameters, fecal nitrogen content, and carcass traits. Three independent trials with 360 pigs were included. A total of 220 pigs (5.32 ± 0.46 kg) were fed ad libitum, either a 14, 16, 18, 20, or 22% CP diet from about 5- to 10-kg (trial 1); 84 pigs (11.27 ± 1.43 kg) were fed either a 12, 14, 16, 18, or 20% CP diet from about 10- to 20-kg (trial 2); and 56 pigs (18.80 ± 2.21 kg) were fed either a 10, 12, 14, 16, or 18% CP diet from about 20- to 30-kg (trial 3). In trial 1, as dietary CP levels increased, the feed-to-gain ratio (F/G) quadratically decreased (p &lt; 0.05) and was minimal at the 18.42% CP level. The average daily feed intake (ADFI) and final body weight (BW) were not affected by dietary CP levels while the fat percentage decreased (p &lt; 0.05). Besides, a linear decrease in slaughter rate (p = 0.06) and a linear increase in bone percentage (p &lt; 0.05), serum urea nitrogen (UN) (P &lt; 0.05), and fecal nitrogen content (p = 0.07) of pigs were observed. In trial 2, as dietary CP levels increased, the average daily gain quadratically increased (p &lt; 0.05) and was maximum at the 16.70% CP level. The slaughter rate linearly decreased (p &lt; 0.05) whereas the skin rate, serum UN, and NH3-N increased (p &lt; 0.05) linearly, as well as fecal nitrogen content (p = 0.06). In trial 3, as dietary CP levels increased, the F/G increased (p &lt; 0.05), while the ADFI and ADG quadratically decreased (p &lt; 0.05) and was maximum at nearly 14.00% CP level. The bone percentage and serum UN increased (p &lt; 0.05) linearly but the slaughter rate decreased (P &lt; 0.05) linearly, and the fecal nitrogen content quadratically decreased (p = 0.07) whereas the albumin increased (p &lt; 0.05) quadratically. Taken together, the optimal dietary CP levels for growth performance of Huanjiang mini-pigs from 5- to 10-kg, 10- to 20-kg, and 20- to 30-kg were 18.42, 16.70, and 14.00%, respectively.


2021 ◽  
Author(s):  
Abdolhamid Karimi ◽  
Mohammad Javad Abarghuei ◽  
Alidad Boostani

Abstract In this investigation, performance, feed efficiency and carcass traits of 48 weaned crossbred Grey Shirazi × Ghezel and purbred Grey Shirazi fattening lambs (mean live weight, 28.5 ± 0.5 kg and age, 115 ± 5 days) were evaluated. Lambs were divided into 4 groups contain the GM group (12 purebred male lambs of Grey Shirazi ewe × Grey Shirazi ram), the GF group (12 purebred female lambs of Grey Shirazi ewe × Grey Shirazi ram), the CM group (12 crossbred male lambs of Grey Shirazi ewe × Ghezel ram) and the CF group (12 crossbred female lambs of Grey Shirazi ewe × Ghezel ram). The dry matter intakes (DMI), average daily gain (ADG) and carcass traits in the lambs were determined. The DMI was greater in CF lambs compared to GF lambs (P < 0.001). Feed conversion ratio (FCR) was higher in male lambs comparing to female lambs (P = 0.001). The final body weight, ADG, live weight at the slaughtering and hot carcass weight (HCW) were lower in GF lambs compared to another lambs (P < 0.001, P = 0.003). Cold carcass weight (CCW) in CM lambs was higher than in other groups. The Longissimus dorsi (LD) area was higher in crossbreed male lambs (P = 0.001). Crossbreeding improved grow performance (final body weight and ADG) in male and female lambs. Carcass trait was improved by crossbreeding especially in male lambs. Sex effect on growth performance and carcass traits was noticeable and male lambs had superior growth.


2020 ◽  
Vol 63 (2) ◽  
pp. 367-376
Author(s):  
Kathrin Elbert ◽  
Neal Matthews ◽  
Ralf Wassmuth ◽  
Jens Tetens

Abstract. A variety of available terminal sire lines makes the choice of terminal sire line complex for the pig producer. Higher birth weights are important for subsequent growth performance and selection for this trait is also necessary in sire lines. The aim was to investigate the effect of sire line, birth weight and gender on growth performance, carcass traits and meat quality. In total 3844 crossbred pigs from Camborough Pig Improvement Company (PIC) dams matched with either a Synthetic (A) or Piétrain (B) sire line were used. Pigs from line A grew faster (p<0.01), showed higher feed intake (p<0.01) and reached a higher final body weight (p≤0.01), but they had a similar efficiency (p=0.179). Leaner carcasses and heavier primal cuts (p<0.001) were observed in pigs from line B. Carcasses from pigs sired by line A had higher meat quality (p<0.001). Males had a higher growth rate (p≤0.05) but had a poorer feed efficiency (p<0.01). Heavier birth weight pigs and females had leaner, higher value carcasses with heavier primal cuts (p<0.001) compared to middle and low birth weight females or males. Sire line by sex interactions was significant for growth (p≤0.05) and carcass traits (p<0.001). Interaction between sire line and birth weight classes were only detected for loin depth (p<0.01). Line A is preferable if the numbers of fatting pigs per fattening place and year should be improved, and line B is an option to increase leanness and carcass primal cuts.


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