scholarly journals Similarity Comparisons of SARS-CoV-2 Samples between Wuhan and G20

2020 ◽  
Author(s):  
Zhenhui Wang ◽  
Mu Qiao ◽  
Jeffrey Zheng

Abstract To analyze SARS CoV-2 genomes, this paper uses a viral genome collected in Wuhan as a basic sequence to select a set of genomes from G20 countries/regions for comparison. Two methods are applied in visualization followed in the A1 and C1 modules of the MAS to provide 1D similarity projection and information entropy approaches. For a pair of two genomes segmented as M segments to calculate base differences corresponding to each segment, a measuring vector can be generated to be projected as a visual map. Refined information will be provided to calculate the information entropy corresponding to base differences. This provides quantitative measures to organize these genomes arranged into a linear order. The first method uses a line chart representation to illustrate relevant differences among genomes 1 by 1 between Wuhan and other regions. The second method uses a histogram representation to observe the information entropy projection results. Significant similarities are observed among the differences with approximately equal entropies with higher similarity. Based on the comparison of gene sequence diagrams between Wuhan and various regions, a difference analysis was carried out.

2021 ◽  
Author(s):  
Zhenhui Wang ◽  
Mu Qiao ◽  
Jeffrey Zheng

Abstract To analyze SARS CoV-2 genomes, this paper uses a viral genome collected in Wuhan as a basic sequence to select a set of genomes from G20 countries/regions for comparison. Two methods are applied in visualization followed in the A1 and C1 modules of the MAS to provide 1D similarity projection and information entropy approaches. For a pair of two genomes segmented as M segments to calculate base differences corresponding to each segment, a measuring vector can be generated to be projected as a visual map. Refined information will be provided to calculate the information entropy corresponding to base differences. This provides quantitative measures to organize these genomes arranged into a linear order. The first method uses a line chart representation to illustrate relevant differences among genomes 1 by 1 between Wuhan and other regions. The second method uses a histogram representation to observe the information entropy projection results. Significant similarities are observed among the differences with approximately equal entropies with higher similarity. Based on the comparison of gene sequence diagrams between Wuhan and various regions, a difference analysis was carried out.


2008 ◽  
Vol 4 (3) ◽  
pp. 275-284 ◽  
Author(s):  
Christophe Sibertin-Blanc ◽  
Nabil Hameurlain ◽  
Omar Tahir

Author(s):  
E, R. Walker ◽  
N. O. Olson ◽  
M. H. Friedman

An unidentified virus, responsible for an arthritic-like condition in chickens was studied by electron microscopy and other methods of viral investigation. It was characterized in chorio-allantoic membrane (CAM) lesions of embryonating chicken eggs and in tissue culture as to: 1) particle size; 2) structure; 3) mode of replication in the cell; and 4) nucleic acid type.The inoculated virus, coated and uncoated, is first seen in lysosomal-like inclusions near the nucleus; the virions appear to be uncoated in these electron dense inclusions (Figure 1), Although transfer of the viral genome from these inclusions is not observable, replicating virus and mature virus crystals are seen in the cytoplasm subsequent to the uncoating of the virions.The crystals are formed in association with a mass of fibrils 50 to 80 angstroms in diameter and a ribosome-studded structure that appears to be granular endoplasmic reticulum adapted to virus replication (Figure 2). The mature virion (Figure 3) is an icosahedral particle approximately 75 millimicrons in diameter. The inner core is 45 millimicrons, the outer coat 15 millimicrons, and the virion has no envelope.


Author(s):  
Shaima’a Dakhel AbdulHassa

Gairdia lamblia is one of parasites that cause intestinal problems within the human body, particularly private travelers and children. In this study a total of (100) diarrheal patients, 20 patients with Giardiasis were identified by fecal antigen. 9 out of 20(20%) of them were infected by fecal antigen, while 9(9%) of them were infected by using the screening general stool examination (GSE). The stool samples were collected from patient how vested the Medical City/ Baghdad and Tikrit teaching Hospital during the period from 1 st may 2018 to 1 February 2019. The results revealing a significant difference (p andlt; 0.05) between the two methods of detection for G. lamblia (Fecal antigen method and GSE). IT has been shown that out of 20 infected individuals 12(12%) were males and 8(8%) were females, indicating regarding no significant deference in the distribution of Giardiasis among genders. In regard the age, our results showed that highest infection rate 8(3.2%) was recorded in the age group (10-19) years, followed by the age group (20-2) years which was 692.4%). In this study five mutations were recorded at position (926, 1094, 1202and 1304), by using tpiA gene sequence method, and tpiB gene was on point mutation change (G254A), in the position (85) of triose phosphate isomease.


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