scholarly journals Effects of Dietary Carbohydrate To Lipid Ratios On Growth Performance, Body Composition, Serum Biochemical Indexes, Lipid Metabolism And Gene Expression of Central Appetite Regulating Factors In Chinese Perch (Siniperca Chuatsi)

2021 ◽  
Author(s):  
Di Peng ◽  
Xu-Fang Liang ◽  
Farui Chai ◽  
Hexiong Feng ◽  
Jiao Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Composition ◽  
Lipid Metabolism ◽  
Growth Performance ◽  
Mrna Expression ◽  
Dietary Carbohydrate ◽  
Crude Lipid ◽  
Siniperca Chuatsi ◽  
Chinese Perch ◽  
Serum Biochemical

Abstract An 8-week feeding trial was conducted to evaluate the effects of dietary carbohydrate to lipid (CHO: L) ratios on growth performance, body composition, serum biochemical indexes, lipid metabolism and gene expression of central appetite regulating factors in Chinese perch (Siniperca chuatsi) (mean initial weight: 12.86 ± 0.10 g). Five isonitrogenous and isoenergetic diets (fish meal, casein as main protein sources) were formulated to contain different graded CHO:L ratio diets ranging from 0.12, 0.86, 1.71, 3.29 and 7.19. Each diet was assigned to triplicate groups of 18 experimental fish for 8 weeks. Our results revealed that final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER) increased with dietary CHO:L ratio from 0.12 to 1.71, and then decreased with further increases in dietary CHO:L ratio. A two-slope broken-line regression analysis based on WGR showed that the optimal dietary CHO: L level for maximum growth performance of fish was 1.60. Crude lipid and crude protein content in the liver and glycogen concentration in the muscle and liver were significantly influenced by the dietary CHO:L ratios (P < 0.05). The lowest crude lipid content in the liver was observed in fish fed the diet with a CHO:L ratio of 1.71(P < 0.05). Dietary CHO:L ratios significantly induced the Glu contents of serum (P < 0.05). The relative expression levels of genes involved in lipid metabolism, such as srebp1 and fas in the liver showed a trend of first decreased and then increased with the increase of dietary CHO:L ratios levels. Appropriate CHO:L ratio in the diet can effectively reduce the accumulation of liver fat. We observed in fish fed the 1.71 CHO:L ratio diet showed higher feed intake, up‐regulated mRNA expression of neuropeptide Y (NPY) and agouti gene-related protein (AGRP), down‐regulated mRNA expression of cocaine-and amphetamine-regulated transcript (CART) and pro‐opiomelanocorticoid (POMC) significantly as compared to control group. Thus, these results provide the theoretical basis for feed formulation to determine the appropriate CHO:L ratio requirement of Chinese perch.

scholarly journals Adaptation of AMPK-mTOR-signal pathways and lipid metabolism in response to low- and high-rapeseed meal diet in Chinese perch (Siniperca chuatsi)

2020 ◽  
Author(s):  
Jiao Li ◽  
Xu-Fang Liang ◽  
Muhammad Shoaib Alam ◽  
Haocan Luo ◽  
Yanpeng Zhang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Growth Performance ◽  
Mrna Expression ◽  
Visceral Adipose Tissue ◽  
Rapeseed Meal ◽  
Whole Body ◽  
Signal Pathways ◽  
Chinese Perch ◽  
Visceral Adipose

Abstract Background: Chinese perch, a carnivorous fish, can accept artificial diet after domestication nowadays, and this farm way will gain high economic interest and sustainability. However, the high content and high quality requirement of dietary protein make it need the high cost in Chinese perch. Therefore, the aim of this study was to explore the effect of fish meal replacement by low- or high-rapeseed meal on growth performance, feeding, lipid and glucose metabolism. Methods: Three experimental diets were formulated with 0, 10% and 30% rapeseed meal, named as control, RSL and RSH, groups respectively. After the 8-week of feeding trial, growth performance, lipid metabolism and AMPK-mTOR-signal pathways were measured. Results: Chinese perch fed with RSH and RSL diets showed significantly decreased WG, SGR, BFR, VSI, MSI and the whole-body crude lipid compared to those fed with the control diet (P < 0.05). Fish in RSL group decreased feed intake, serum LDL-C, hepatic mRNA expression of LPL, PEPCK and phosphorylated Grb10 (P < 0.05). In visceral adipose tissue, mRNA expression of FAS, SREBP1, ACC1, HL, CPT1 and PEPCK were all significantly down-regulated (P < 0.05). Fish in RSH group showed phosphorylated AMPK, hepatic mRNA expression of SREBP1, ACC1, FAS, PPARα and CPT1 were down-regulated, while HSL, G6PD and PC were up-regulated (P < 0.05). In visceral adipose tissue, mRNA expressions of SREBP1, LPL, CPT1 and PEPCK were down-regulated, while mRNA expression of HSL was up-regulated (P < 0.05). Conclusions: Chinese perch fed with RSL and RSH diets showed decreased fat deposition in viscera. Fish fed with low level of rapeseed meal diet ate less diet, which caused inhibited lipid metabolism in the liver and visceral adipose tissues.Fish fed with high level of rapeseed meal diet inhibited hepatic FA synthesis, activated lipolysis, hence reducing Acetyl-CoA pool. In turn, β-oxidation were inhibited, glycolysis was activated, thus lipid accumulation was decreased. In visceral adipose tissue, lipid uptake was inhibited, caused inhibited FA synthesis, β-oxidation, glycerol synthesis, and improved lipolysis.

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