Femtomolar And Locus-Specific Detection of N6-Methyladenine In DNA By Integrating Double-Hindered Replication And Nucleic Acid-Functionalized MB@Zr-MOF

Abstract In this study, a novel electrochemical biosensor was constructed for ultrasensitive and locus-specific detection of N6-Methyladenine (m6A) in DNA using double-hindered replication and nucleic acid-coated methylene blue (MB)@Zr-MOF. Based on the combination of m6A-impeded replication and AgI-mediated mismatch replication, this mode could effectively stop the extension of the strand once DNA polymerase encountered m6A site, which specifically distinguish the m6A site from natural A site in DNA. Also, Zr-MOF with high porosity and negative surface potential features was carefully chose to load cationic MB, resulting a stable and robust MB@Zr-MOF electrochemical tag. As a result, the developed biosensor exhibited a wide linear range from 1 fM to 1 nM with detection limit down to 0.89 fM. Profiting from the high sensitivity and selectivity, the biosensing strategy revealed good applicability, which had been demonstrated by quantitating m6A DNA at specific site in biological matrix. Thus, the biosensor provides a promising platform for locus-specific m6A DNA analysis.

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Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF

Journal of Nanobiotechnology ◽

10.1186/s12951-021-01156-0 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Qingyuan Zheng ◽

Tong Wang ◽

Xinmin Li ◽

Husun Qian ◽

Xintong Bian ◽

...

Keyword(s):

Nucleic Acid ◽

Dna Polymerase ◽

Dna Analysis ◽

Electrochemical Biosensor ◽

High Sensitivity ◽

High Porosity ◽

Specific Detection ◽

Sensitivity And Selectivity ◽

Negative Surface ◽

A Site

AbstractIn this study, a novel electrochemical biosensor was constructed for ultrasensitive and locus-specific detection of N6-Methyladenine (m6A) in DNA using double-hindered replication and nucleic acid-coated methylene blue (MB)@Zr-MOF. Based on the combination of m6A-impeded replication and AgI-mediated mismatch replication, this mode could effectively stop the extension of the strand once DNA polymerase encountered m6A site, which specifically distinguish the m6A site from natural A site in DNA. Also, Zr-MOF with high porosity and negative surface potential features was carefully chose to load cationic MB, resulting a stable and robust MB@Zr-MOF electrochemical tag. As a result, the developed biosensor exhibited a wide linear range from 1 fM to 1 nM with detection limit down to 0.89 fM. Profiting from the high sensitivity and selectivity, the biosensing strategy revealed good applicability, which had been demonstrated by quantitating m6A DNA at specific site in biological matrix. Thus, the biosensor provides a promising platform for locus-specific m6A DNA analysis. Graphical Abstract

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Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions

Journal of Nucleic Acids ◽

10.1155/2012/247280 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Kwabena Sarpong ◽

Bhaskar Datta

Keyword(s):

Nucleic Acid ◽

High Sensitivity ◽

Covalent Modification ◽

Fluorescence Signal ◽

Nucleic Acid Binding ◽

Sensitivity And Selectivity ◽

Target Molecules ◽

Diagnostic Applications ◽

The Stability ◽

Simple Format

The binding affinity and specificity of nucleic acid aptamers have made them valuable candidates for use as sensors in diagnostic applications. In particular, chromophore-functionalized aptamers offer a relatively simple format for detection and quantification of target molecules. We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. Aptamers varying in size and structure and targeting a range of molecules have been used in conjunction with commercially available chromophores to indicate and quantify the presence of cognate targets with high sensitivity and selectivity. Our assay precludes the covalent modification of nucleic acids and relies on the differential fluorescence signal of chromophores when complexed with aptamers with or without their cognate target. We also evaluate factors that are critical for the stability of the complex between the aptamer and chromophore in presence or absence of target molecules. Our results indicate the possibility of controlling those factors to enhance the sensitivity of target detection by the aptamers used in such assays.

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Easy-to-Fabricate K2Pd(SO3)2-Dyed Polyester Fabric with Highly Selective and Fast Response to Carbon Monoxide

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.19418 ◽

2021 ◽

Vol 21 (8) ◽

pp. 4400-4405

Author(s):

Junyeop Lee ◽

Nam Gon Do ◽

Dong Hyuk Jeong ◽

Sae-Wan Kim ◽

Maeum Han ◽

...

Keyword(s):

Carbon Monoxide ◽

Color Change ◽

High Sensitivity ◽

Cost Effective ◽

Fast Response ◽

Polyester Fabric ◽

High Porosity ◽

Sensing Material ◽

Sensitivity And Selectivity ◽

And Performance

Carbon monoxide (CO) is an odorless, colorless, tasteless, extremely flammable, and highly toxic gas. It is produced when there is insufficient oxygen supply during the combustion of carbon to produce carbon dioxide (CO2). CO is produced from operating engines, stoves, or furnaces. CO poisoning occurs when CO accumulates in the bloodstream and can result in severe tissue damage or even death. Many types of CO sensors have been reported, including electrochemical, semiconductor metal-oxide, catalytic combustion, thermal conductivity, and infrared absorption-type for the detection of CO. However, despite their excellent selectivity and sensitivity, issues such as complexity, power consumption, and calibration limit their applications. In this study, a fabricbased colorimetric CO sensor is proposed to address these issues. Potassium disulfitopalladate (II) (K2Pd(SO3)2) is dyed on a polyester fabric as a sensing material for selective CO detection. The sensing characteristics and performance are investigated using optical instruments such as RGB sensor and spectrometer. The sensor shows immediate color change when exposed to CO at a concentration that is even lower than 20 ppm before 2 min. The fast response time of the sensor is attributed to its high porosity to react with CO. This easy-to-fabricate and cost-effective sensor can detect and prevent the leakage of CO simultaneously with high sensitivity and selectivity toward CO.

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Early stage of nucleic acid electrochemistry. Detection of DNA damage in X-ray-irradiated rats

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc2011151 ◽

2011 ◽

Vol 76 (12) ◽

pp. 1799-1810 ◽

Cited By ~ 3

Author(s):

Emil Paleček

Keyword(s):

Dna Damage ◽

Nucleic Acid ◽

Dna Analysis ◽

Early Stage ◽

High Sensitivity ◽

Strand Breaks ◽

X Ray ◽

Dna And Rna ◽

Oscillographic Polarography ◽

Radiation Induced

First papers on electroactivity of DNA and RNA were published more then 50 years ago. For about 8 years oscillographic polarography at controlled a.c. (OP, proposed by J. Heyrovský already in 1941) was the method of choice for DNA analysis. Since approximately 1954 Robert Kalvoda developed OP for wide application in various fields. It is shown that already before 1960 it was possible to detect damage to DNA in X-ray-irradiated rats by means of OP. DNA samples from irradiated animals produced significantly larger OP anodic guanine signal indicating changes in the DNA structure. At present, radiation-induced strand breaks and damage to bases in DNA can be electrochemically detected at high sensitivity.

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Aptamer and graphene‐based electrochemical biosensor for dopamine determination with high sensitivity and selectivity

Micro & Nano Letters ◽

10.1049/mnl.2013.0626 ◽

2013 ◽

Vol 8 (12) ◽

pp. 903-905 ◽

Cited By ~ 10

Author(s):

Luyang Jiao ◽

Guangjian Lu ◽

Chunxiao Zhang ◽

Yan Yuan ◽

Junxiang Wang ◽

...

Keyword(s):

Electrochemical Biosensor ◽

High Sensitivity ◽

Sensitivity And Selectivity ◽

Dopamine Determination

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Recent Development of Aptamer Sensors for the Quantification of Aflatoxin B1

Applied Sciences ◽

10.3390/app9112364 ◽

2019 ◽

Vol 9 (11) ◽

pp. 2364 ◽

Cited By ~ 10

Author(s):

Yongmei Jia ◽

Guohua Zhou ◽

Peilian Liu ◽

Zhiguo Li ◽

Biao Yu

Keyword(s):

Nucleic Acid ◽

Food Quality ◽

Aflatoxin B1 ◽

High Selectivity ◽

High Sensitivity ◽

Nucleic Acid Sequence ◽

Recent Developments ◽

Contaminated Food ◽

Sensitivity And Selectivity ◽

Labor Consuming

Aflatoxin B1 (AFB1) is one of the most frequently-found mycotoxins in contaminated food. As the content of mycotoxins is particularly low in food, the development of probes to detect AFB1 in foods with high sensitivity and selectivity is an urgent social need for the evaluation of food quality. Numerous techniques have been developed to monitor AFB1. Nevertheless, most of them require cumbersome, labor-consuming, and sophisticated instruments, which have limited their application. An aptamer is a single, short nucleic acid sequence that is capable of recognizing different targets. Owing to their unique properties, aptamers have been considered as alternatives to antibodies. Aptasensors are considered to be an emerging strategy for the quantification of aflatoxin B1 with high selectivity and sensitivity. In this review, we summarize recent developments in colormetric, electrochemical, SERS, and fluorescent aptasensors for the quantification of AFB1. Finally, the perspectives and current challenges of aptasensors for AFB1 are outlined.

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Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity

Chemical Communications ◽

10.1039/c0cc02782k ◽

2010 ◽

Vol 46 (47) ◽

pp. 9043 ◽

Cited By ~ 84

Author(s):

Zhiyuan Fang ◽

Jing Huang ◽

Puchang Lie ◽

Zhuo Xiao ◽

Chuanyan Ouyang ◽

...

Keyword(s):

Aqueous Solution ◽

Nucleic Acid ◽

High Sensitivity ◽

Lateral Flow ◽

Sensitivity And Selectivity

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Schottky diodes based on 2D materials for environmental gas monitoring: a review on emerging trends, recent developments and future perspectives

Journal of Materials Chemistry C ◽

10.1039/d0tc04840b ◽

2021 ◽

Author(s):

Minu Mathew ◽

Chandra Sekhar Rout

Keyword(s):

Gas Sensing ◽

2D Materials ◽

Schottky Diodes ◽

High Sensitivity ◽

Future Perspectives ◽

Working Temperature ◽

Emerging Trends ◽

Gas Sensing Properties ◽

Recent Developments ◽

Sensitivity And Selectivity

This review details the fundamentals, working principles and recent developments of Schottky junctions based on 2D materials to emphasize their improved gas sensing properties including low working temperature, high sensitivity, and selectivity.

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