Systemic Evaluation of Mechanism of Cytotoxicity in Human Colon Cancer HCT-116 Cells of Silver Nanoparticles Synthesized Using Marine Algae Ulva Lactuca Extract.

Abstract In the current study, biogenic silver nanoparticles (U-AgNPs) were synthesized using marine green macro-algal Ulva Lactuca extract, and evaluated mechanism behind the anticancer activity against the Human colon cancer (HCT-116). The novel biogenic U-AgNPs were characterized using various physiochemical techniques. The TEM micrographs confirmed the spherical morphology of synthesized U-AgNPs, with a mean size of 8–14 nm. FTIR Spectra analysis of U-AgNPs confirmed Ulva Lactuca extract bioactive molecules presence over U-AgNPs surface as a stabilizing agent, thereby improving biocompatibility. The cytotoxicity study revealed the dose dependent cell death in colon cancer cells with no loss of viability in normal human colon epithelial cells. Furthermore, the fluorescence micrographs of nucleus staining assay revealed the DNA fragmentation and nucleus condensation of cancer cells treated with U-AgNPs, indicating an apoptosis-mediated cell death. The western bolt and RT-PCR analysis of U-AgNPs treated cancer cells showed the rise in proapoptotic markers (P53, Bax, and P21) and decline in anti-apoptotic markers (Bcl-2), thus confirming the p53-dependent apoptosis mediated cell death in HCT-116. Overall, our study concluded that novel biogenic U-AgNPs nanoparticles, synthesized using marine green macro-algal Ulva Lactuca extract showed efficient anticancer activity against HCT-116 cell line and hence could work as potential therapeutic agent for targeted anti-cancer therapy.

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Fucoidan and Alginate from the Brown Algae Colpomenia sinuosa and Their Combination with Vitamin C Trigger Apoptosis in Colon Cancer

Molecules ◽

10.3390/molecules27020358 ◽

2022 ◽

Vol 27 (2) ◽

pp. 358

Author(s):

Reem Al Monla ◽

Zeina Dassouki ◽

Nouha Sari-Chmayssem ◽

Hiba Mawlawi ◽

Hala Gali-Muhtasib

Keyword(s):

Colon Cancer ◽

Cell Death ◽

Vitamin C ◽

Cancer Cell ◽

Brown Algae ◽

Human Colon ◽

Bioactive Molecules ◽

Dapi Staining ◽

Human Colon Cancer ◽

Hct 116

Brown seaweeds are producers of bioactive molecules which are known to inhibit oncogenic growth. Here, we investigated the antioxidant, cytotoxic, and apoptotic effects of two polysaccharides from the brown algae Colpomenia sinuosa, namely fucoidan and alginate, in a panel of cancer cell lines and evaluated their effects when combined with vitamin C. Fucoidan and alginate were isolated from brown algae and characterized by HPLC, FTIR, and NMR spectroscopy. The results indicated that highly sulfated fucoidans had higher antioxidant and cytotoxic effects than alginate. Human colon cancer cells were the most sensitive to the algal treatments, with fucoidan having an IC50 value (618.9 µg/mL−1) lower than that of alginate (690 µg/mL−1). The production of reactive oxygen species was increased upon treatment of HCT-116 cells with fucoidan and alginate, which suggest that these compounds may trigger cell death via oxidative damage. The combination of fucoidan with vitamin C showed enhanced effects compared to treatment with fucoidan alone, as evidenced by the significant inhibitory effects on HCT-116 colon cancer cell viability. The combination of the algal polysaccharides with vitamin C caused enhanced degeneration in the nuclei of cells, as evidenced by DAPI staining and increased the subG1 population, suggesting the induction of cell death. Together, these results suggest that fucoidan and alginate from the brown algae C. sinuosa are promising anticancer compounds, particularly when used in combination with vitamin C.

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Systemic Evaluation of Mechanism of Cytotoxicity in Human Colon Cancer HCT-116 Cells of Silver Nanoparticles Synthesized Using Marine Algae Ulva lactuca Extract

Journal of Inorganic and Organometallic Polymers and Materials ◽

10.1007/s10904-021-02133-8 ◽

2021 ◽

Author(s):

Diptikanta Acharya ◽

Sagarika Satapathy ◽

Kanti Kusum Yadav ◽

Prathap Somu ◽

Gitanjali Mishra

Keyword(s):

Colon Cancer ◽

Silver Nanoparticles ◽

Marine Algae ◽

Human Colon ◽

Ulva Lactuca ◽

Human Colon Cancer ◽

Hct 116 ◽

Hct 116 Cells

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Characterisation of carboxylate nanocrystal cellulose /silver nanoparticles from eucalyptus pulp and its antitumour activity

10.21203/rs.3.rs-331735/v1 ◽

2021 ◽

Author(s):

Pasakorn Jutakridsada ◽

Somnuk Theerakulpisut ◽

Mika Sillanpää ◽

Khanita Kamwilaisak

Keyword(s):

Colon Cancer ◽

Silver Nanoparticles ◽

Anticancer Activity ◽

Human Skin ◽

Silver Ions ◽

Human Colon ◽

Skin Melanoma ◽

Human Colon Cancer ◽

Controllable Release ◽

Hct 116

Abstract Cellulose nanocrystal (CNC) has a high prospect in the biomedical field due to its favourable biocompatibility, stability, and modifiable properties, which render it potentially useful as a template for silver deposition nanoparticles (Ag NPs). In this research work, the aim was to prepare AgNPs on carboxylate nanocrystal cellulose (cCNC) in a minimised process and decrease the number of chemical reagents and with a stable anchor and controllable release Ag + by hydrothermal reaction. The controlled release of Ag+ was also determined. The anticancer activity of Ag-cCNC on HCT116 (Human colon cancer cells) and SK-MEL-2 (Human Skin Melanoma cells) were investigated. Silver nanoparticles were uniformly deposited onto cCNC with a 28.0% yield and a particle size of ∼15 nm. The release rate of silver ions was monitored for 32 days by ICOES analysis. It was found that Ag-cCNC induced silver ions to release slowly, which displayed a controllable release of silver ions with a rate of 0.1-0.2% per 2 days and a longterm release of 245 days. Also, Ag-cCNC showed anticancer activity against both HCT 116 (Human colon cancer cell) and SK-MEL-2 (Human Skin Melanoma cell) at 35±4.04% and 20±7.68% cell viability, respectively. Ag-cCNC showed promising higher 15% anticancer HCT 116 efficacy than cisplatin, but it was lower 18% in anticancer SK-MEL-2. This study provides a novel, sustainable, simple and straightforward way with environmentally friendly to produce and use natural composite material from Eucalyptus as an antitumour drug.

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Ultrasound-enhanced biosynthesis of uniform ZnO nanorice using Swietenia macrophylla seed extract and its in vitro anticancer activity

Nanotechnology Reviews ◽

10.1515/ntrev-2021-0044 ◽

2021 ◽

Vol 10 (1) ◽

pp. 572-585

Author(s):

Darren Yi Sern Low ◽

Camille Keisha Mahendra ◽

Janarthanan Supramaniam ◽

Loh Teng Hern Tan ◽

Learn Han Lee ◽

...

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Hexagonal Wurtzite Structure ◽

Colon Cancer Cells ◽

Swietenia Macrophylla ◽

Zno Nps ◽

Human Colon Cancer ◽

Hct 116

Abstract In this study, ultrasonically driven biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Swietenia macrophylla seed ethyl acetate fraction (SMEAF) has been reported. X-ray powder diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analyses confirmed the presence of a pure hexagonal wurtzite structure of ZnO. Field emission scanning electron microscope images revealed the formation of uniquely identifiable uniform rice-shaped biologically synthesized ZnOSMEAF particles. The particle sizes of the biosynthesized NPs ranged from 262 to 311 nm. The underlying mechanisms for the biosynthesis of ZnOSMEAF under ultrasound have been proposed based on FTIR and XRD results. The anticancer activity of the as-prepared ZnOSMEAF was investigated against HCT-116 human colon cancer cell lines via methyl thiazolyl tetrazolium assay. ZnOSMEAF exhibited significant anticancer activity against colon cancer cells with higher potency than ZnO particles prepared using the chemical method and SMEAF alone. Exposure of HCT-116 colon cancer cells to ZnOSMEAF promoted a remarkable reduction in cell viability in all the tested concentrations. This study suggests that green sonochemically induced ZnO NPs using medicinal plant extract could be a potential anticancer agent for biomedical applications.

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